• Environmental Analysis Health and Toxicology
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• v.30 no.sup
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• pp.7.1-7.10
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• 2015

Objectives For successful adoption of legislation controlling registration and assessment of chemical substances, it is important to obtain sufficient toxicological experimental evidence and other related information. It is also essential to obtain a sufficient number of predicted risk and toxicity results. Particularly, methods used in predicting toxicities of chemical substances during acquisition of required data, ultimately become an economic method for future dealings with new substances. Although the need for such methods is gradually increasing, the-required information about reliability and applicability range has not been systematically provided. Methods There are various representative environmental and human toxicity models based on quantitative structure-activity relationships (QSAR). Here, we secured the 10 representative QSAR-based prediction models and its information that can make predictions about substances that are expected to be regulated. We used models that predict and confirm usability of the information expected to be collected and submitted according to the legislation. After collecting and evaluating each predictive model and relevant data, we prepared methods quantifying the scientific validity and reliability, which are essential conditions for using predictive models. Results We calculated predicted values for the models. Furthermore, we deduced and compared adequacies of the models using the Alternative non-testing method assessed for Registration, Evaluation, Authorization, and Restriction of Chemicals Substances scoring system, and deduced the applicability domains for each model. Additionally, we calculated and compared inclusion rates of substances expected to be regulated, to confirm the applicability. Conclusions We evaluated and compared the data, adequacy, and applicability of our selected QSAR-based toxicity prediction models, and included them in a database. Based on this data, we aimed to construct a system that can be used with predicted toxicity results. Furthermore, by presenting the suitability of individual predicted results, we aimed to provide a foundation that could be used in actual assessments and regulations.

• Proceedings of the Korea Contents Association Conference
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• 2009.05a
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• pp.449-453
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• 2009

The primary purpose of this study was to probe the relationship between negotiating efforts on leisure restriction and participation in leisure sports. This study has sampled for 26 days from 2009 February 9th to March 6th 250 adult at the age of 19 living in capital area using the Purposive Sampling Method. The study has then divided these samples into 3groups which are : public people, members of an association club, members of a club. To exclude non-participant of leisure sports, the researcher has visited the leisure sports facilities to sampling survey. The collected data were put into the SPSSWIN 15.0 program to be Factor Analysis, Reliability Analysis, Multiple Regression Analysis, Path Analysis. The results are: 1) Negotiating efforts on leisure sports affect the frequency of leisure sports participation: thus, the more effort on leisure activity and eagerness to change the more frequent the participation rate becomes. 2) Negotiating efforts on leisure sports affect the length of leisure sports participation. The means that the recharging through leisure activity and regulatory the intensity can extend the length of participation. 3) Negotiating efforts on leisure sports affect the intensity of participation. The more effort on leisure activity result in higher intensity of participation.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.29 no.3
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• pp.15-24
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• 2006

As Gyoto Agreement, the Climate Change Agreement, has become effective, total weight restriction on waste has started in each country and environment issue becomes one of the most important global issues. Therefore, environmental logistics became important matter, and industries need to establish production system for environmental conscious products and services. In this paper, we compare and analyze environmental conscious logistics activity(ECLA) that affect Carbon Dioxide Discharge(CDD) and packaging waste caused by logistics activity in Korea and Japan. We also propose the optimal alternative to the problem by this comparison and analysis.

• Journal of Microbiology and Biotechnology
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• v.7 no.5
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• pp.293-298
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• 1997

A 4.6-kb HindIII fragment encompassing the complete 140-kDa ${\alpha}$-amylase gene of Lactobacillus amylovorus B 4540 was cloned into pBR322 by the shot gun method. Southern blotting and restriction mapping for the insert were performed. The recombinant 9.0-kb plasmid, pFML1, conferred ${\alpha}$-amylase activity to E. coli and Lactococcus lactis hosts when introduced by electroporation. SDS-PAGE and zymography confirmed the production of 140-kDa ${\alpha}$-amylase and its proteolytic degradation products with enzyme activity in transformants. Total ${\alpha}$-amylase activity of E. coli $DH5{\alpha}$ cells harboring pFML1 was 1.8 units and most activity was detected from cell pellets. Total enzyme activity of L. lactis subsp. lactis MG1363 transformant was five to ten-fold lower than that of E. coli cell but more than half of the activity was detected in the culture supernatant.

• Korean Journal of Microbiology
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• v.32 no.3
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• pp.208-214
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• 1994

We screened many species from a wide variety of bacterial genera for a new type II restriction endonuclease. The purification and characterization of SolI from a soil isolate, Streptoverticillium olivoverticillatum are described here. The enzyme turned out to be an isoschizomer of BamHI. It recognized the hexanucleotide sequence of 5'-G$\downarrow$GATCC-3' and cleaved as in dicated by the arrow, generating a 4 base 5' extension. Unlike its isoschizomer, BamHI, the activity was sensitive to dam methylation within the recognition sequence. Following ammonium sulfate fractionation of the crude extract, heparin-agarose and Affi-gel Blue column chromatography were employed to purify the enzyme. SolI required at least 0.2 mM of $MgCl_2$ for the cleavage to occur. The enzyme exhibited its maximal activity in the absence of NaCl, but was inhibited completely in the presence of 120 mM NaCl. The pH and temperature optima for activity were pH 8.6 and $40^{\circ}C$, respectively. The molecular weight of SolI was estimated to be 43,000 Da by Superose-12 gel filtraion chromatography.

• 한국노년학
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• v.30 no.1
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• pp.93-108
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• 2010

This study conducted a secondary analysis by using original data of performed by Korea Institute for Health and Social Affairs to determine factors affecting health-related quality of life for the elderly aged over 65 years living in Korea. The survey was conducted in 2005 and it evaluated totally 34,152 cases. Among them 3074 cases investigating the elderly aged over 65 years were selected for this study and finally 2036 cases were included in it by excluding cases with no answer or a wrong answer. The results were as follows. In the illness days, the average of the whole subjects was 11.05 days and it was longer when subjects were female and older, had lower educational background and lower family income, did not have spouses and jobs and were covered by medical aid as medical security. In the years of activity restriction, the average of the total subjects was 3.48 years and it was increased when they were older, had lower educational background and lower family income, did not have spouses, lived in detached houses and were covered by medical aid as medical security. Subjective health condition of the total subjects was 2.64 points out of 5 points in average. The scores were higher when subjects were male, younger, had better educational background, spouses, jobs and more family income, lived in multi-family living houses and were covered by corporate insurance as medical security. The average of total quality of life of the subjects was 2.61 points out of 3 points and it was found to be higher when subjects were male, younger, had better educational background, spouses, jobs and more family income and were covered by corporate insurance as medical security. It was decreased with higher illness days, higher years of activity restriction and lower subjective health condition. For the effects of the factors related to quality of life, subjective health condition showed the largest influence.

• Journal of Photoscience
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• v.9 no.2
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• pp.252-254
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• 2002

Djungarian hamsters show distinct seasonal rhythms in several physiological parameters. One of them is daily torpor that occurs in winter with decreased body temperature (about 1O-20$^{\circ}$C) during daytime. Daily torpor is induced by short-day photoperiod, food restriction and castration. But the mechanism to induce daily torpor has not been clarified. In the present study, we tried to clarify the process of daily torpor induction in detail. Adult male hamsters were kept in long photoperiod and high temperature (LP-HT) before the experiment and, thereafter, the animals were transferred to short photoperiod and low temperature (SP-LT), and they were kept in this condition for about six months. The daily rhythms of locomotor activity and body temperature were recorded every three-minutes by using the Minimitter telemetry system. Locomotor activity and body temperature showed very closely synchronized rhythms. All animals under LP-HT showed daily rhythms with higher locomotor activity and body temperature in nighttime than in daytime. Under SP-LT, there were two types of animals with and without showing daily torpor. Thus, they have individual differences in the response to SP -LT.

• The Journal of Korean Physical Therapy
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• v.26 no.2
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• pp.130-135
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• 2014

Purpose: The purpose of this study was to examine the impairments of body structures, activity limitation, and participation restriction. In addition, we wanted to provide basic data on correlation between impairments of body structures, activity, and participation in Disabled Persons Living at Home. Methods: After selection of 128 people with physical disabilities more than the third level and brain damage disabilities living at home, we conducted testing for impairments of body structures, activity, and participation, according to the ICF checklist. Results: Impairments of body structures was highest in the upper and lower extremity. 2) Mobility, domestic life, and self care were more limited. 3) Also, the structure related to movement showed correlation with mobility, domestic life, and self care. Conclusion: We observed differences in participation and activities of persons with disabilities Living at Home depending on the impairments of body structures. After thorough review of the status of Disabled Persons Living at Home, we decided to appropriate support and social services.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1986.12a
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• pp.525.1-525
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• 1986

To clone ${\beta}$-glucosidase gene from Cellulomonas sp. a gene library was constructed using E. coli JM83 pUC9. Among 2,500 pseudotransformants obtained, 20 clones developed yellow color on the p-nitrophenyl- -D-glucopyranoside filter paper These 20 clones were classified into three groups based on the results of activity staining using nondenaturating polyacrylamide gel electrophoresis and restriction enzyme digestions. Among the three groups, only one group containing pCEl plasmid has specificity for cellobiose.

• The Journal of the Korean dental association
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• v.12 no.10
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• pp.767-770
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• 1974

A 12 years old patient having rampant dental caries on permanent dentition has been treated under operative procedures. In addition to this operative treatment the nutritional survey and the caries activity was checked, and performed the oral hygiene instruction to the patient. As the consequences of above procedures the following results were obtained: 1. The familial history and eating habits checkings were helpful for better dental health guide and caries control. 2. The restriction of mono & disaccharide intake and topical application of 8% stannous fluoride at least two times revealed excellent control effect for recurrent dental caries.