• International Journal of Industrial Entomology and Biomaterials
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• 제31권2호
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• pp.79-84
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• 2015

A new cecropin-like antimicrobial peptide (Px-CLP) gene was isolated from the immunechallenged larvae of the swallowtail butterfly, Papilio xuthus, by employing annealing control primer (ACP)-based GeneFishing PCR. The full-length cDNA of Px-CLP is 310 nucleotides encoding a 70 amino acid precursor that contains a putative 22-residue signal peptide, a 4-residue propeptide, a presumed 37-residue mature peptide, and an uncommon 7-residue acidic pro-region at the C-terminus. The deduced amino acid sequence of Px-CLP showed significant identities with other Lepidopteran cecropin D type peptides. RT-PCR revealed that the Px-CLP transcript was detected at significant level after injection with bacterial lipopolysaccharide (LPS). The peptides with or without C-terminal acidic sequence region were synthesized on-solid phage and submitted to antibacterial activity assay. The synthetic 37-mer peptide (Px-CLPa), which removed C-terminal acidic sequence region, was showed exclusively antibacterial activity against E. coli ML35; meanwhile, a 44-mer peptide (Px-CLPb) with C-terminal acidic peptide region was not active. This result suggests that Px-CLP is produced as a larger precursor containing a C-terminal pro-region that is subsequently removed by C-terminal modification.

• Journal of Microbiology
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• 제37권3호
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• pp.175-179
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• 1999

To elucidate the role of vir-box in the expression of the virE gene, the vir-box was modified by site-directed mutagenesis and tested for ${\beta}$-galactosidase activities. A, C, T T, A, C substitutions at -62, -63, and -65 positions, destroying the 5'-region of the vir-box and A T at position -55, destroying the 3'-region of the vir-box respectively, showed only 17% promoter activity. When the vir-box was modified to contain perfect dyad symmetry structure (DSR) by the substitutions T, G A, T at -60 an d-61 positions, ${\beta}$-glactosidase activity increased 302%. These results indicate that the 5' and 3'-region of vir-box as well as the imperfect DSR of the vir-box itself may play a very important role in the regulation of virE gene expression.

• 미생물학회지
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• 제30권6호
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• pp.488-494
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• 1992

Three clones containing mouse CD4 gene were prepared using AKR genomic cosmid library. The role of 6, 500 bp 5' flanking region of the first exon of the AKR CD4 gene in tissue or developmental stage specific expression of CD4 has been studied. The deletion constructs containing various amounts of CD4 5' flanking sequences were prepared, and they were transfected into the cell lines representing different cell types or developmental stages of CD4 expression. Study of the reporter gene expression revealed that at least 1, 700 bp of 5' flanking region did retain promoter activity for CD4 expression. This area did not seem to contain enhancer activity for a full expression of CD4. However, the putative promoter interacted with other tissue specific enhancer sequence and showed the tissue specificity of the enhancer element.

• Journal of Astronomy and Space Sciences
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• 제17권2호
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• pp.189-198
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• 2000

RS Cvn형 4상성 SZ Psc의 광도곡선에 나타난 뒤틀림 현상을 규명하기 위하여 자외선 영역의 방출선과 가시광선 영역의 광도곡선을 분석하였다. 자외선 영역에서 IUE 고분산 분광선 중에서 MgII 방출선의 강도를 조사하여, 채충 활동은 주로 Kl IV 별에서 일어나고 있는 것을 확인하였다. 한편, 가시광선 영역에서 나타난 광도 곡선의 뒤틀림 현상은 Kl IV별에 혹점이 존재하는 혹점 모델을 이용하여 분석한 결과 SZ Psc의 뒤틀린 광도 곡선을 잘 설명할 수 있었다. 그 결과 SZ Psc에 대한 완전한 측광학적인 해를 최초로 제시하였다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XIII)
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• pp.794-798
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• 2003

천연추출물로서 미백 재료로 널리 쓰이고 있는 옥용산에 대해서 in vitro 미백활성을 UV 흡수능, tyrosinase 저해활성 그리고 free radical 소거활성을 측정함으로서 검정해 보았다. 옥용산 UV-B와 C 영역에 걸쳐서 높은 자외선 흡수능을 보이는 것으로 관찰이 되었으며 0.8 % 이상의 농도에서 80% 이상의 tyrosinase 저해활성을 가지는 것을 관찰하였다. 또한 조협, 승마, 감송, 정향, 찹쌀분과 옥용산의 추출물은 높은 free radical 소거능을 가지는 것으로 확인되었다.

• 수산해양교육연구
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• 제25권4호
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• pp.958-972
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• 2013

The purpose of this study is to analyze on high school teachers' stage of concern(SoC) on 'Creative-Experience Activity' in 2009 revised national curriculum and investigate the improvements of their levels. The subjects for this study were 234 high school teachers through out all part of the Korea country. The instrument for this study was developed according to the Hall & Hord(2006)'s stage of concerns questionnaire of Stage of Concern. The Data were analyzed by Profiling of teachers' concern and one-way ANOVA. The major findings of this study were as follows: First, 85.25% of the high school teachers were in stage of Awareness. 6.34% of teachers were in stage of Information. Most of high school teachers' concern about 'Creative-Experience Activity' was generally 'non-user' stage that unconcerned. Second, there were not significant differences in teachers' stage of concern according to their sex and teaching career except for region. In region, the teacher in metropolis tent to interest getting new information than small and midium size city. We need to make an effort to transfer from 'non-user' stage to 'early-user' stage of and 'impact' stage. To do so, we may sublate too much curriculum revise and start teacher training for 2009 revised 'creativity-experience activity' curriculum. The result of the study provide that there are not curriculum implementation success without teachers' concern about revised curriculum.

• Journal of Microbiology and Biotechnology
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• 제15권3호
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• pp.678-682
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• 2005

The promoter region of a carbon starvation gene isolated from Pseudomonas putida was cloned and analyzed for its potential use for in situ bioremediation and bioprocessing. We constructed a recombinant plasmid pMKD101 by cloning the 0.65 kb promoter region of the gene into the promoter proving vector, pMK301, which contains the lacZ for ${\beta}$-galactosidase activity as a reporter gene. pMKD101 was transformed into the wild-type P. putida MK1, resulting in P. putida RPD101, and analyzed for ${\beta}$-galactosidase activity under different culture conditions. When RPD101 was grown on the minimal medium plus $0.1\%$ glucose as a sole carbon source in batch cultures, ${\beta}$-galactosidase activity was found to be 3.2-fold higher during the stationary phase than during the exponential phase. In chemostat cultures, ${\beta}$-galactosidase activity was found to be 3.1-fold higher at the minimal growth rate (dilution rate=$0.05\;h^{-1}$) than at the maximal growth rate (dilution rate=$0.173;h^{-1}$). The results suggest that a carbon starvation promoter can be utilized to maximize the expression of a desired gene under nutrient limitation.

• 음성과학
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• 제10권1호
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• pp.95-105
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• 2003

This paper describes a new parameter for voice activity detection which serves as a front-end part for automatic speech recognition systems. The new parameter called run-ratio is derived from the runs test statistic which is used in the statistical test for randomness of a given sequence. The run-ratio parameter has the property that the values of the parameter for the random sequence are about 1. To apply the run-ratio parameter into the voice activity detection method, it is assumed that the samples of an inputted audio signal should be converted to binary sequences of positive and negative values. Then, the silence region in the audio signal can be regarded as random sequences so that their values of the run-ratio would be about 1. The run-ratio for the voiced region has far lower values than 1 and for fricative sounds higher values than 1. Therefore, the parameter can discriminate speech signals from the background sounds by using the newly derived run-ratio parameter. The proposed voice activity detector outperformed the conventional energy-based detector in the sense of error mean and variance, small deviation from true speech boundaries, and low chance of missing real utterances

• 지역사회간호학회지
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• 제31권spc호
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• pp.563-576
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• 2020

Purpose: The purpose of this study is to identify personal factors, social factors, and environmental factors related to physical activity in older adults in urban and non-urban areas. Methods: We used source data from the 2017 Community Health Survey. The subjects of this study included some older adults aged 65 and over, and analyzed the data of 23,043 older adults living in the urban and 34,063 older adults living in the non-urban area. Results: The common factors influencing physical activity in older adults by region include current smoking and drinking, BMI, sleep duration, and subjective health status, help with neighbors, frequency of meeting with neighbors and friends, participation in social and leisure activities, and falls experience (p<.001). However, the living environment, public transport satisfaction, and medical service use significantly associated with physical activity for only older adults living in the urban area (p<.001). Conclusion: In order to improve physical activity in older adults in the community, it is necessary to consider not only the improvement of individual factors that practice health behaviors but also health promotion strategies that take into account social and environmental factors because there are environmental differences among regions.

• Journal of Life Science
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• 제10권1호
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• pp.20-23
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• 2000

Mono-ADP-ribosylation, catalyzed by ADP-ribosyltransferases, is a post-translational modification of proteins in which the ADP-ribose moiety of NAD is transferred to an acceptor protein. Previously, we have identified and cloned a glycosylphosphatidylinositol-linked ADP-ribosyltransferase (Yac-1) from mouse lymphoma cells. Yac-1 enzyme contains three regions (region I,II,III) similar to those found in several bacterial toxins and vertebrate ADP-ribosyltransferases. Site-directed mutagenesis was performed to verify the role of Glu 233 in region III. Mutants E233Q, E233D and E233A were inactive for ADP-ribosyltransferase activity. Thus Glu 233 in Yac-1 is essential for enzyme activity, suggesting that Glu 233 in Glu-rich motif near the carboxy terminus plays a catalytic role in ADP-ribosyltransferase activity.