• Bulletin of the Korean Chemical Society
• /
• 제32권1호
• /
• pp.183-185
• /
• 2011

Adenylate kinase-like activity and phosphotransferase-like activity from $F_1$-ATPase of Escherichia coli was revealed by $^{31}P$ NMR spectroscopy. Incubation of F1-ATPase with ADP in the presence of $Mg^{2+}$ shows the appearance of $^{31}P$ resonances from AMP and Pi, suggesting generation of AMP and ATP by adenylate kinase-like activity and the subsequent hydrolysis to Pi. Incubation of $F_1$-ATPase with ADP in the presence of methanol shows additional peak from methyl phosphate, suggesting phosphotransferase-like activity of $F_1$-ATPase. Both adenylate kinase-like activity and phosphotransferase-like activity has not been reported from $F_1$-ATPase of Escherichia coli. $^{31}P$ NMR could be a valuable tool for the investigation of phosphorous related enzyme.

• 한국원자력학회:학술대회논문집
• /
• 한국원자력학회 2004년도 추계학술발표회 발표논문집
• /
• pp.75-76
• /
• 2004

• Mycobiology
• /
• 제49권6호
• /
• pp.559-581
• /
• 2021

The coastal marine ecosystems of Vietnam are one of the global biodiversity hotspots, but the biodiversity of marine fungi is not well known. To fill this major gap of knowledge, we assessed the genetic diversity (ITS sequence) of 75 fungal strains isolated from 11 surface coastal marine and deeper waters in Nha Trang Bay and Van Phong Bay using a culture-dependent approach and 5 OTUs (Operational Taxonomic Units) of fungi in three representative sampling sites using next-generation sequencing. The results from both approaches shared similar fungal taxonomy to the most abundant phylum (Ascomycota), genera (Candida and Aspergillus) and species (Candida blankii) but were different at less common taxa. Culturable fungal strains in this study belong to 3 phyla, 5 subdivisions, 7 classes, 12 orders, 17 families, 22 genera and at least 40 species, of which 29 species have been identified and several species are likely novel. Among identified species, 12 and 28 are new records in global and Vietnamese marine areas, respectively. The analysis of enzyme activity and the checklist of trophic mode and guild assignment provided valuable additional biological information and suggested the ecological function of planktonic fungi in the marine food web. This is the largest dataset of marine fungal biodiversity on morphology, phylogeny and enzyme activity in the tropical coastal ecosystems of Vietnam and Southeast Asia. Biogeographic aspects, ecological factors and human impact may structure mycoplankton communities in such aquatic habitats.

• 한국수소및신에너지학회논문집
• /
• 제22권1호
• /
• pp.29-34
• /
• 2011

This study examined the effect of environmental variables, such as the NaOH concentration and solution temperature, on the rate of hydrogen generation from NaOH solutions through the corrosion of used aluminum cans as a potential candidate material for the safe and economic production of hydrogen. Corrosion of the used aluminum cans was promoted by increasing the NaOH concentration and solution temperature because of the loss of aluminum passivity. The measured rate of hydrogen generation from the NaOH solutions increased with increasing NaOH concentration due to the catalytic activity of NaOH in the hydrolysis process. However, at higher solution temperatures, the rate of hydrogen generation rate was less affected by the NaOH concentration than that at lower temperature.

• 가족자원경영과 정책
• /
• 제22권4호
• /
• pp.1-15
• /
• 2018

The purposes of this study is to analyze the leisure behaviors of single households in Korea on the basis of generation, and identify leisure characteristics or factors that influence levels of happiness in these households. The sample comprised 1,144 single householders, among whom 74 belong to the teenage generation, 288 are of the eco generation, 154 belong to generation X, 104 are baby boomers, and 524 are of the older generation. Various leisure characteristics were compared across the generations, namely, conditions for leisure, degree of leisure participation, level of work-leisure balance, persistence of participation, and type of activity. Results showed differences in leisure characteristics and leisure factors that influence levels of happiness among the generations.

• 정보교육학회논문지
• /
• 제21권6호
• /
• pp.647-656
• /
• 2017

디지털 스토리텔링은 학생들이 스토리를 기획 구성하고, 다양한 디지털 기술을 사용하여 결과물을 제작하는 기회를 제공한다. 디지털 스토리텔링 활동을 통하여 학생들은 디지털 세대에 요구되는 창의성, 의사소통 능력, 비판적 사고력, 협동과 같은 역량들을 신장시킬 수 있다. 본 연구는 학생들이 스토리의 프로슈머가 되어 스토리의 기획 및 구성은 물론 기획한 스토리를 디지털 자료로 제작할 수 있도록 안내하는 디지털 스토리텔링 활동 모형을 개발하였다. 모형은 '활동 준비', '스토리 기획 및 구성', '스토리텔링 자료 제작', '자료 발표 및 평가' 등과 같은 4가지 단계로 구분하고, 각 단계에 대한 활동 지침들을 제공하였다. 또한 각 단계별 활동을 돕기 위하여 스토리 기획서, 스토리 테이블, 팀간 평가지, 개별 성찰지 등의 양식들을 제공하였다.

• 한국융합학회논문지
• /
• 제9권7호
• /
• pp.149-154
• /
• 2018

본 논문은 Lavender, Tea tree, Eucalyptus, Lemongrass 4종의 천연에센셜오일의 Streptpccous mutans 에 의한 치석형성억제 효과에 대하여 연구해 보고자 하였다. Streptpccous mutans 에 의한 치석형성 억제 활성 측정결과인 Lavender 에센셜 오일의 MAC (Minium anti-adhesive concentration, 최소부착저지 농도)가 1.0 % 이고 Tea tree 에센셜 오일, Eucaliptus 에센셜 오일, lemongrass 에센셜 오일의 MAC는 0.5 % 로 나타났다. 에센셜오일의 농도를 달리하여 Streptpccous mutans와 혼합한 배양액을 항온조에서 배양한 후 용액의 pH를 측정하여 최소부착 저지농도 이하에서 산의 생성으로 pH가 낮아진 것을 확인할 수 있었고, 이 결과는 에센셜 오일이 Streptpccous mutans 에 의한 산의 생성을 억제하는 효과가 있다는 것이다. 이러한 결과들로부터 천연에센셜오일 중 특히 Tea tree, Eucalyptus, Lemongrass는 치석생성을 억제하는 천연소재로 구강관리 제품개발에 활용할 수 있는 가능성을 확인할 수 있었다.

• Natural Product Sciences
• /
• 제17권2호
• /
• pp.90-94
• /
• 2011

An in vitro bioassay-guide revealed that the methanol (MeOH) extract of the whole plant of Patrinia saniculaefolia (Valerianaceae) showed cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX) dual inhibitory activity by assessing their effects on the production of prostaglandin $D_2$ ($PGD_2$) and leukotriene $C_4$ ($LTC_4$) in mouse bone marrow-derived mast cells (BMMCs). Phytochemical study of the MeOH extract of this plant led to the isolation of twelve compounds; ${\beta}$-farnesene (1), squalene (2), nardostachin (3), patridoid I (4), patridoid II (5), patridoid II-A (6), oleanolic acid (7), oleanonic acid (8), 23-hydroxyursolic acid (9), oleanolic acid 3-O-${\alpha}$-L-arabinopyranoside (10), oleanolic acid 3-O-${\beta}$-D-glucopyranoside (11), oleanolic acid 3-O-[${\beta}$-D-xylopyranosyl-(1${\rightarrow}$3)-${\beta}$-D-(6-O-butyl)glucuronopyranoside] (12). Among the compounds, 4 and 5 strongly inhibited both the COX-2-dependent $PGD_2$ generation with $IC_{50}$ values of 8.7 and 13.6 ${\mu}M$, respectively, and the generation of $LTC_4$ in the 5-LOX dependent phase with $IC_{50}$ values of 41.7 and 46.9 ${\mu}M$, respectively, which suggest that the anti-inflammatory activity of P. saniculaefolia might occur in part via the inhibition of both $PGD_2$ and $LTC_4$ generation by 4 and 5.

• 대한약학회:학술대회논문집
• /
• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
• /
• pp.278.1-278.1
• /
• 2002

In the previous study, we reported that NQ12, a vitamin K antagonist. showed a potent antithrombotic and antiplatelet activities. In order to elucidate the antiplatelet activity of NQ12. we investigated the effect of NQ12 on arachidonic acid cascade parameters such as cPLA2. cyclooxygenase (COX), and the downstream production such as TxA2, PGD2 and 12-HETE. N012 inhibited COX activity in a concentration-dependent manner in U937 cells. (omitted)

• 한국의류산업학회지
• /
• 제15권3호
• /
• pp.358-363
• /
• 2013

Due to recent changes in increased life expectancy, economic power and social development, the new senior generation (focusing on people in their fifties) represents a new market power with strong competitiveness. The new senior generation is the generation after the baby boomers, with characteristics that include a high-level of education, increased income, a high-level of consumption, increased assets, self-fulfillment desires and individuality. Demands in the senior fashion industry are expected to gradually increase and the underwear industry that targets the new senior generation is expected to grow rapidly. These demands will require product development for the new senior generation based on emotional and socio-cultural characteristics. The current new senior underwear market is under the process of R&D with a focus on body shape changes; however, there are limited studies on emotional and socio-cultural approaches. This study formulates a basic design plan for different age groups based on an analysis of the situation of bra wear (current inconveniences, demand and needs) by women in their fifties and over. Subsequently, a functional active bra top that considers activity and comfort was proposed for women in their fifties, an all-in-one foundation bra top that shapes the body was proposed for women in their sixties, and comfort bra tops that are comfortable and easy to put on and take off were proposed for women in their seventies and over.