• Title/Summary/Keyword: Activity Determination

Search Result 773, Processing Time 0.023 seconds

A Study of Determination of Minimum Detectable Activity at Measuring of Pu Isotopes in Environmental Samples (환경시료 중 Pu 측정에서 최소검출방사능 농도 산출에 대한 연구)

  • Lee, Myung-Ho;Cho, Young-Hyun;Choi, Gun-Sik;Lee, Chang-Woo;Shin, Hyun-Sang
    • Analytical Science and Technology
    • /
    • v.14 no.1
    • /
    • pp.64-71
    • /
    • 2001
  • In this paper, the formula of lower limit of detection considering the measuring time of sample and background was derived using the basic concepts of lower limit of detection. Among parameters affecting the determination of MDA value, the MDA values were calculated with variation of amount of sample and measuring time of sample and background. The results of adequate division between the measuring time of sample and that of background studied in this research make it possible to evaluate confidence limits on the radio analytical results in the environmental sample.

  • PDF

Determination of the Kinetic Properties of Platy cod in D for the Inhibition of Pancreatic Lipase Using a 1, 2-Diglyceride- Based Colorimetric Assay

  • Zhao, Hai-Un;Kim, Yeong-Shik
    • Archives of Pharmacal Research
    • /
    • v.27 no.9
    • /
    • pp.968-972
    • /
    • 2004
  • A 1, 2-diglyceride-based multi-step colorimetric assay to measure the pancreatic lipase activ-ity was applied for the determination of the kinetic profiles of the lipase inhibition with a slight modification and the validity verification. With this assay method, our study revealed that platy-codin D, one of major constituents of Platycodi Radix, inhibits the pancreatic lipase activity in a competitive type, with the value of $K_1$ being 0.18${\pm}$0.02 mM. In addition, PO has affected the val-ues of $K_{m}$, app/ and $K_{cat}$/$K_{m}$ in a dose-dependent manner. The results shed a meaningful light on how PO mediates lipid metabolism in the intestinal tracts. On the other hand, since the revised assay is sensitive, rapid, and does not affect the accuracy to the kinetic properties, it is applica-ble not only to evaluation of the kinetic properties of the pancreatic lipase, but also to high-throughput screening of pancreatic lipase activity.

Determination of Cholinesterase Activities in Sera of Human and Different Species of Animals by Means of the Propionylthiocholine-Dithiobis Procedure (Propionylthiocholine-Dithiobis 방법에 의한 사람과 몇몇 동물 혈청내의 Cholinesterase 활성도 측정)

  • Kim, Soon-tae;Kim, Sung-hoon;Do, Jae-cheul;Lee, Young-ho;Mo, Ki-cheul;Huh, Rhin-sou
    • Korean Journal of Veterinary Research
    • /
    • v.27 no.1
    • /
    • pp.153-155
    • /
    • 1987
  • Determination of cholinesterase activity is a routine practice in many laboratories to detect the influence of cholinesterase inhibiting drugs such as organophosphate and carbamate insecticides. Among many different methods to determine the cholinesterase activity, the present method was the most recent, simple and accurate one for routine test in clinics. The results obtained in sera of human and the different species of animals by means of the present method were as follows: $5.76{\pm}1.12U/ml$ in human, $3.37{\pm}0.83U/ml$ in german shepherd, $0.61{\pm}0.18U/ml$ in rat, $14.91{\pm}3.10U/ml$ in mouse, $1.55{\pm}0.51U/ml$ in chicken, $0.28{\pm}0.11U/ml$ in slaughtered cattle and $0.50{\pm}0.10U/ml$ in slaughtered pig.

  • PDF

Changes in Liver and Serum Guanase Activity following Administration of Carbon Tetrachloride in Rats (四鹽化炭素에 依한 肝損傷時 Guanase 活性 變動)

  • Kang, Hoe Yang;Yoon, Chong Guk
    • Journal of Environmental Health Sciences
    • /
    • v.12 no.1
    • /
    • pp.25-38
    • /
    • 1986
  • This paper was intented to charily the cause of an increase of serum guanase activity in rats following injection of $CCI_4$. The cause of increasing serum guanase was focused on the change of guanase activities in both serum and liver, and additionally, these results were compared with the previously known alanine aminotransferase (ALT). Concomitantly the microscopic investigation on the histologic changes, and the determination of lipid peroxides of liver were combined in this experiment for a correlation to observe that the activity of guanase would be effected by the various degree of hepatic injury induced by $CCI_4$. The serum levels of guanase were increased about 2 fold in the fatty change stage (3-12 days), 5.2 fold representing the peak value in necrosis stage (21days), 4.5 fold in early cirrhosis stage (48 days), and 2 fold in severe cirrhosis stage (92 days). These changes of serum guanase activity showed similar patterns to those of ALT activity and lipid peroxides in liver cell. The changes of liver guanase activities showed an increase, whereas ALT activities in liver were markedly decreased. It is likely that the increase of serum guanase activity is based on the excess leaking of guanase into blood by the result of accelerated enzyme synthesis in liver cell of $CCI_4$ intoxicated rats. In addition, the possibility could not be ruled out, however, that the increase of serum guanase activity would be caused by the alteration of membrane permeability.

  • PDF

Study on Bromobenzene Metabolism in Rats with Liver Damage (흰쥐에 있어서 간손상 정도에 따른 Bromobenzene 대사)

  • 신중규
    • Toxicological Research
    • /
    • v.13 no.4
    • /
    • pp.371-376
    • /
    • 1997
  • To compare the severe liver damage with the slight one on the bromobeazene metabolism in rats, the animal group described as B7 group was induced the stage of slight liver damage with 7 times bromobenzene injection every other day (400 mg/Kg body wt. i.p.), whereas B40 group was induced that of more severe liver damage with bromobeazene 40 times injection as identified with determination of serum levels of alanine aminotransferase(ALT) activity and the histopathological findings. In the present experimental animal model, the decreasing rate of glutathione(GSH) and the increasing rate of glutathione S-transferase activity to the control group were higher in B7 group than B40 group. Furthermore the single dose of bromobenzene was injected to the two groups and sacrificed at 8hr and the hepatic aniline hydroxylase(AH) activity, GSH content and GST activity were determined. The increasing rate of AH activity to the control was lower in B40 group than B7 group and the decreasing rate of GSH to the control was also lower in B40 than B7 group. Moreover, B7 group showed the increased activity of hepatic GST to the control whereas B40 group showed the decrease activity of the enzyme. And Vmax value in GST was more decreased in B40 group than B7 group.

  • PDF

The study of critical indicator development for establishing patient classification system in the Intensive Care Unit (중환자실에서의 환자분류체계 확립을 위한 결정지표 개발에 관한 연구)

  • Kim, Kil-Youb;Jang, Keum-Seoung
    • Journal of Korean Academy of Nursing Administration
    • /
    • v.8 no.3
    • /
    • pp.475-488
    • /
    • 2002
  • Purpose : The purpose of this study is to establish a basis of patient classification in the ICU by selecting the determination critical indicator of special nursing activities that show high interrilation with daily total nursing care time. Method : This study is composed of the six steps. The first step is the listing direct nursing activities in the ICU. The last step is the determination indicator of each group were selected on the basis of their relationship to the daily total nursing care time of each patient classification group and each nursing activity. Result : Result shows that: 1. direct nursing activities in the ICU are 149 items of 13 territories. 2. the average time and frequency for each direct nursing activities 3. total direct nursing care time of 42 patients in ICU for 2 days. According to the results of the Cluster analysis, the first group is 10 people, the second group is 13 people, the third group is 16 people, the fourth group is 3 people. 4. Determination critical indicator is the item that is r>0.6(p<0.05) of Pearson Correlation between each patient daily total nursing care time and 149 items of nursing activities. The nursing activities selected were as follows: 2 items in the first group, 17 items in the second group. 16 items in the third group, 8 items in the fourth group. Conclusion : This study can help future studies which measure nursing activities standard time or assigns value to nursing activities time.

  • PDF

Nephrotoxicity Assessment by Determination of Urinary ${\gamma}$-Glutamyltranspeptidase ( ${\gamma}$-GTP) and N-Acetyl-$\beta$-D-Gluosa- minidase (AGS) in Rat (Rat에서 뇨중 ${\gamma}$-Glutamyltranspeptidase와 N-Acetyl-$\beta$-D-glucosaminidase 측정에 의한 신독성 평가에 관하여)

  • Kim Young-Ho;Lee Chang-Woo
    • Journal of Veterinary Clinics
    • /
    • v.7 no.2
    • /
    • pp.471-487
    • /
    • 1990
  • Present experiment was performed in order to establish the optimum conditions for quantitation of ${\gamma}$-GTP and AGS activities in rat urine and investigate the applicability of the these enzymes in experimental assessment of nephrotoxicity in rats. The results obtained were as follows. 1. The optimal pH of Tris-BCI buffer containing glycylglycine for determination of urinary ${\gamma}$-GTP activity was 7.6(37$^{\circ}C$). 2. The Michaelis constant of urinary ${\gamma}$-GTP ranged from 1.1 to 1.2 mmol/$\ell$. 3. The optimal pH of citrate buffer for determination of urinary AGS activity was 3.6(37$^{\circ}C$). 4. The Michaelis constant of urinary AGS ranged from 0.8 to 0.9mmo1/$\ell$. 5. Coefficient of variance for within-run imprecision of urinary ${\gamma}$-GTP ranged from 3.8 to 6.4% and that of urinary AGS ranged from 2.5 to 4.1%. 6. There was no significant difference between gel-filtered samples and crude samples in the mean activity of urinary ${\gamma}$-GTP and the intra-individual differences by gel-filtration were either increased or decreased. Mean values of ${\gamma}$ -GTP activities in gel-filtered samples and crude samples were 1570 and 1590 U/$\ell$, repectively. 7. The mean activity of urinary AGS increased significantly after gel-filtration and all the individual urines revealed higher activities after gel-filtration. 8. ${\gamma}$-GTP and AGS activities were linear to 135 and 7U/$\ell$, respectively. 9. Urinary ${\gamma}$-GTP and AGS excretion before administration of potassium dichromate were 22.1 ${\pm}$ 11.2 and 0.5${\pm}$0.2 U/24hrsㆍkg body weight respectively and increased significantly to 102.3${\pm}$44.5 and 5.8${\pm}$3.30/24hrsㆍkg body weight respectively within 24 hours after administration. 10. BUN increased continuously from 24 hours following exposure to potassium dichromate in all 10 rats. From these findings it is concluded that the urinary ${\gamma}$-GTP and AGS excretions are early and sensitive indicators for nephrotoxicity assessment in rat.

  • PDF

Determination of Phytochemical Contents and Biological Activities from the Fruits of Elaeagnus multiflora

  • Lee, Jin-Hwan;Seo, Weon-Taek;Cho, Kye-Man
    • Preventive Nutrition and Food Science
    • /
    • v.16 no.1
    • /
    • pp.29-36
    • /
    • 2011
  • The purpose of this study was to investigate the phytochemical contents and biological activities, including $\alpha$-glucosidase inhibitory activity and antioxidant activity, from the fruits of Elaeagnus multiflora using different solvent systems. The 50% ethanol extract of this species showed the most potent $\alpha$-glucosidase inibitory activity (88.5%) at a concentration of 1 mg/mL. Moreover, this extract also displayed the strongest antioxidant activity in each assay, showing 96.3% in DPPH scavenging activity, 98.2% in ABTS scavenging activity, and 2.5% in reducing power. The highest total phytochemical contents, including flavan-3-ols, phenolic acids, and flavonoids, were observed in the 50% ethanol extract. Interestingly, flavan-3-ols (3.150 mg/g) were detected at a significantly higher total content than those of phenolic acids (0.380 mg/g) and flavonoids (not detected). Additionally, the contents of individual phytochemicals showed remarkable differences, especially the epicatechin gallate (2.008 mg/g) and gallic acid (0.099 mg/g), which were the predominant constituents of each phytochemical type. Therefore, our results suggest that the 50% ethanol extract of E. multiflora fruits has strong biological activities, which are correlated with high phytochemical contents.

Advanced Lipid Extraction Method for the Determination of the Phospholipase D Activity

  • Yon, Chang-Suek;Lee, Min-Ho;Oh, Doo-Yi;Kim, Dal-Soo;Lee, Ki-Sung;Han, Joong-Soo
    • Archives of Pharmacal Research
    • /
    • v.26 no.6
    • /
    • pp.478-481
    • /
    • 2003
  • Phospholipase D is a ubiquitous enzyme that plays an important role in various lipid mediated cellular signaling pathways and produces rare phospholipids, phosphatidylethanol or phosphatidylbutanol, instead of phosphatidic acid with unique catalytic activity transphosphatidylation in the presence of primary alcohols. The reaction products, phosphatidylethanol or phosphatidylbutanol are used as markers of in vitro phospholipase D activity in many studies. For the sensitive detection of the phospholipase D products, we developed an advanced lipid extraction method that facilitates recovery of the compounds. With the new method, the activity change of phosaholipase D by agonists could be detected more easily and the recovery rate was also increased. The increase of detected enzyme activity change was about double fold compared to the conventional lipid extraction method. This method provides selective force for the phospholipase D products in the extraction procedure.

Purification and Characterization of Lipoxygenase from Melania Snail (다슬기에서 추출한 Lipoxygenase의 정제와 특성)

  • 이양봉;신의철;김병철;양지영;장영진
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.27 no.5
    • /
    • pp.808-812
    • /
    • 1998
  • Melania snail(Semisulcopira bensoni) is used as ingredient in Korean traditional soup and nutritional foods. Generally, lipoxygenase in several food products may produce off-flavors during their processing and storage. Therefore, the inactivation of lipoxygenase is required to make the better extracts from Melania sanil. Also, the quality on freshness of Melania snail may be evaluated by lipoxygenase activity. The lipoxygenae activity was the highest at 40~60% saturation among several concentrations in salting-ouot saturated solution of ammonium sulfate. The partial purification of lipoxygenase was successfully obtained by Sephacryl S-200 gel chromatography. The first peak among three peaks for protein determination showed the highest activity of lipoxygenase in 13~16 fractions among 100 fractions. The highest peak of lipoxygenase activity by ion exchange chromatography was shown at 0.1M NaCl. In the purification step, the specific activity was 20.8U/mg and activity yield was 19.8%. The optimum pH and temperature were pH6.0~8.0 and 3$0^{\circ}C$, respectively. Molecular weight of the lipoxygenase was estimated about 35kDa by SDS-PAGE.

  • PDF