• 제목/요약/키워드: Activity Determination

검색결과 773건 처리시간 0.03초

HPLC를 이용한 고추 및 고추 가공품의 비타민 A 함량 측정 (Use of HPLC for the Determination of Provitamin A Carotenoids in Red Peppers)

  • 김영남
    • Journal of Nutrition and Health
    • /
    • 제25권5호
    • /
    • pp.389-396
    • /
    • 1992
  • Provitamin A carotenoids($\alpha$- $\beta$-carotene lutein capsanthin) cintents in various red pepper and red pepper products were analyzed by HPLC and UV/VIS spectroscopy. The amounts of $\beta$-carotene and capsanthin were at its highest in powdered red pepper and lutein in red pepper leaves. There are little $\alpha$-carotene present in all samples analyzed. So vitamin A activity was calculated only from $\beta$-carotene content. Capsanthin and $\beta$-carotene contents of red pepper were higher than those of green pepper whereas lutein content of red pepper was lower than that of green pepper. whereas lutein content of red pepper was lower than that of green pepper. Vitamin A activity from colorimetric analysis was higher than that from chromatographic method and results were not generally in accordance with the vitamin A data presented in current food composition tables. Vitamin A activities in retinol equivalent(R. E. ) of green pepper red pepper powdered red pepper fermented red pepper paste and red pepper leaves were 70, 570, 1, 340, 140, 980 R. E respectively.

  • PDF

A Dipstick-Type Electrochemical Immunosensor for The Detection of The Organophosphorus Insecticide Fenthion

  • Cho, Young-Ae;Cha, Geun-Sig;Lee, Yong-Tae;Lee, Hye-Sung
    • Food Science and Biotechnology
    • /
    • 제14권6호
    • /
    • pp.743-746
    • /
    • 2005
  • A dipstick-type immunochemical biosensor for the detection of the organophosphorus insecticide fenthion was developed using a screen-printed electrode system as an amperometric transducer with polyclonal antibodies against fenthion as a bioreceptor. The assay of the biosensor involved competition between the pesticide in the sample and pesticide-glucose oxidase conjugate for binding to the antibody immobilized on the membrane. This was followed by measurement of the activity of the bound enzyme by the supply of the enzyme substrate (glucose) and amperometric determination of the enzyme reaction product ($H_2O_2$). The activity of the bound enzyme was inversely proportional to the concentration of pesticide. The optimized sensor system showed a linear response against the logarithm of the pesticide concentration ranging from $10^{-2}$ to $10^3\;{\mu}g/L$.

황금탕(黃芩湯)의 항균작용(抗菌作用) (Antimicrobial Activity of Scute Decoction)

  • 이인란;박흥순
    • 생약학회지
    • /
    • 제18권4호
    • /
    • pp.249-253
    • /
    • 1987
  • Antimicrobial activities of three different extracts from experimentally prepared scute decoction, commercial scute decoction and an extract from Scutellaria baicalensis were evaluated and their inhibitory potencies were compared. Both the extracts from scute decoction and Scutellaria showed rather strong antimicrobial activities against E. coli and St. aureus at the low concentration of 2.5 mg/ml. On the other hand, the extract of commercial scute decoction was found to show an inhibitory effect at higher concentration (75 mg/ml). All preparations did not inhibit the fungal growth of Aspergillus niger. Both Sh. dysenteriae (Subgroup A) and Sh. boydii (Subgroup C) which were used for determination of the strain differences in Shigelia spp. had almost the same susceptibility to the extract from sucute decoction.

  • PDF

공급사슬경영에서의 협업적 리스크 관리의 최적화 (Optimization of collaborative risk management in supply chain management)

  • 정장화;이영해;정정우
    • 한국경영과학회:학술대회논문집
    • /
    • 대한산업공학회/한국경영과학회 2002년도 춘계공동학술대회
    • /
    • pp.456-463
    • /
    • 2002
  • Nowadays. risk management in the enterprise is considered as the important activity. Risk management ran be defined as the activity which is the analysis of risk factors related to damages, the estimation of the magnitude of risk, and the determination of investment to protect damage in a company. Initially, risk management was originated in financial areas. But the concept of risk has been expanded in the enterprise. Most companies have extended their activities in various areas. In this tendency, most activities must be considered in supply chain So, risk management must be ronsidered as the concept in the viewpoint of supply chain. The framework of risk management in supply chain and the related mathematical model are represented in this paper. Risk management in supply chain ran provide a positive opportunity not only to protect various damages, but also to improve the relationship between partners.

  • PDF

Lactobacillus casei의 phospho-$\beta$-galactosidase 유전자의 대장균내 분자클로닝 (Molecular cloning of phospho-$\beta$-galactosidase gene of lactobacillus casei in escherichia coli)

  • 문경희;박정희;최순영;이유미;김태한;김연수;민경희
    • 미생물학회지
    • /
    • 제27권3호
    • /
    • pp.188-193
    • /
    • 1989
  • Gene for lactose catabolism in Lactobacillus casei SW-M1 was encoded by a 60Kb metabolic plasmid. A derivative of only 10kb, pPlac 15 of recombinant plasmid, was constructed by introducing into pBR322 and was cloned into E. coli using restriction endonuclease Pst I. A 10kb insery DNA in plasmid pBR322 was identified as a gene encoded phospho-$\beta$-galactosidase by the determination of enzyme activity. Phospho-$\beta$-galactosidase was apparently expressed in E. coli. The enzyme activities of cell-free extract from transformant E. coli HB101 carrying pPLac 15 DNA were not different from that of L. casei as a donor strain on the basis of enzyme properites. However, specific activity of phospho-$\beta$-galactosidase in the cloned strain with Lac $Y^{-}$ phenotype of E. coli HB101 was lower than that in L. casei strain.

  • PDF

황금과 황백 추출물의 MRSA와 Candida에 대한 항균활성 (Antimicrobial Activities of Scutellaria baicalensis and Phellodendron amurense Against MRSA and Candida)

    • 미생물학회지
    • /
    • 제40권1호
    • /
    • pp.17-22
    • /
    • 2004
  • 항생제 내성균들의 등장으로 새로운 항생제의 개발이 요구되고 있으나 항생제의 개발은 많은 시간과 인력과 개발비를 필요로 한다. 이 문제를 해결하기 위한 방법의 하나로 전통적으로 감염질환의 치료에 사용되어온 식물인 황금과 황백 추출물의 methicillin-resistant Staphylococcus aureus(MRSA)에 대한 항균활성 보유 여부를 조사하였다. 황금의 메탄올 추출물은 MRSA와 Candida에 대하여 높은 항균 활성을 나타내었으며 황백 추출물보다 활성이 높아 앞으로 새로운 항균제로의 개발 및 사용 가능성이 있을 것으로 보여진다.

Isolation and Structure Determination of Coumarin Derivatives from the Roots of Angelica dahurica

  • Kim, Ji-Yeon;Son, Jong-Keun;Song, Dong-Keun
    • Journal of Evidence-Based Herbal Medicine
    • /
    • 제3권1호
    • /
    • pp.35-41
    • /
    • 2010
  • From the roots of Angelica dahurica Bentham et Hooker (Umbelliferae), three known coumarin derivatives have been isolated and identified as 8-(2-hydroxy-3-methoxy-3-methylbutyloxy) psoralen, 5,8-di(2,3-dihydroxy-3-methylbutyloxy) psoralen, 9-[3-($\beta$-D-glucopyranosyloxy)-2-hydroxy-3-methylbutoxy]-7H-furo[3,2-g][1]benzopyran-7-one. This is the first report of the occurrence of these compounds in this plant. These three compounds were tested for activity in septic shock model. Among these compounds, 2 showed relatively strong preventive activity against septic shock.

  • PDF

Electrostatic Interaction Between Oligopeptides and Phosphate Residues by Determination of Absolute Raman Intensities

  • Kye-Taek Lim
    • Bulletin of the Korean Chemical Society
    • /
    • 제12권3호
    • /
    • pp.286-289
    • /
    • 1991
  • The changed isotropic absolute Raman intensities of the phosphate residue in the complexes of positive charge oligopeptides, lys-lys, arg-arg, lys-aromat-lys, negative charge diethyl phosphoric acid (DEP) and polyriboadenylic acid{poly(rA)} were reported and discussed. Our measurements showed that the absolute intensities of phosphate stretch vibration in complexes were different according to the reaction partners. Due to the partial electrical charge and molecular structure of oligopeptides for the complex formation lysine can interact more strongly than arginine when the reaction partners have short chain and no steric hindrance. Owing to these reasons the intensity of phosphate stretching vibration is very sensitive according to the circumstance of reaction. From our results we could suggest that we can discriminate any one of the the lysine and arginine in the complicated biological molecule during interaction between nucleotides and proteins. The activity of reaction of two basical oligopeptides is not quite similar for complex formation in aqueous solution. The activity of dipeptides depends upon the structure of molecule and environment for complex formation. Aromatic ring contributes to electrostatic interaction in complexes. The amount of the absolute intensity for pure stacking interaction is smaller than electrostatic interaction in macromolecular complexes.

Radioactivation Analysis in Clinical and Biochemical Analysis

  • Pijck, J.
    • 약학회지
    • /
    • 제7권4호
    • /
    • pp.75-85
    • /
    • 1963
  • Radioactivation analysis is based upon irradiation of nuclei with elementary particles. In many cases the interaction gives rise to radioactive nuclei, which can be detected and identified by the emitted radiation. If we irradiate under identical conditions an unknown quantity of a certain atomic species and a known quantity of the same species, the following relatio will exist between the induced radioactivities and the irradiated masses: $\frac{Activity standard}{activity unknown}$=$\frac{Mass standard}{Mass unknown}$ From this equation the mass of the unknown can be easily calculated. This relatively siple technique (abstract made from the irradiating facility) allows quantitative determination of about 60 elements with a sensibility that most of the time considerably exceeds the sensibility of conventional analysis techniques. General review articles from Gordon and Meinke should be consulted for further detail.

  • PDF

감마선 처리에 의한 방풍, 백지의 저장성 연구 (Studies on the Storage Characteristics of Angelica dahuricae Radix, Glehnia littoralis Radix Treated with Gamma-irradiation)

  • 김수민;김은주
    • 대한본초학회지
    • /
    • 제24권1호
    • /
    • pp.1-7
    • /
    • 2009
  • Objectives : The purpose of this study is to investigate on storage characteristics of Herbal materials treated with Gamma-irradiation(10 KGy). Methods : This experiments were carried out by field survey and storage characteristics were carried out by physicochemical determination. Results : Antimicrobial activity of oriental medicine materials(Angelica dahurica Radix, Glehnia littoralis Radix) were examined, together with investigation of effects on gamma-irradiation. Oriental medicine materials contaminated in microbial cell were tend to be pinhole in packaging materials(polypropylene) by bug and Larva. At the same time, PVDC (polyvinylldichloride) package also was founded in bug and Larva by microbial contamination during storage in room temperature. Conclusions : In conclusion, it is very desirable to dose gamma-irradiation(10 KGy) in these oriental medicine materials in order to prevent microbial activity.