• 한국화재소방학회:학술대회논문집
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• 한국화재소방학회 2012년도 춘계학술발표회 초록집
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• pp.202-205
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• 2012

• Environmental Engineering Research
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• 제23권1호
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• pp.62-69
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• 2018

In the present study, long-term heavy metals (HMs) contaminated soil samples from a well-known Pb/Zn smelting area in the southwest of China were collected, and physicochemical and biological characteristics of these samples were evaluated. Soil samples contained different concentrations of HMs, namely Pb, Zn, Cu, and Cd. Enzyme activity analyses combined with microcalorimetric analysis were used for soil microbial activity evaluation. Results showed that two soil samples, containing almost the highest concentrations of HMs, also shared the greatest microbial activities. Based on correlation coefficient analysis, high microbial activity in heavily HMs contaminated soil might be due to the high contents of soil organic matter and available phosphorus in these samples. High-throughput sequencing technique was used for microbial community structure analysis. High abundance of genera Sphingomonas and Thiobacillus were also observed in these two heavily contaminated soils, suggesting that bacteria belonging to these two genera might be further isolated from these contaminated soils and applied for future studies of HMs remediation. Results of present study would contribute to the evaluation of microbial communities and isolation of microbial resources to remediate HMs pollution.

• 지역사회간호학회지
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• 제21권1호
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• pp.101-109
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• 2010

Purpose: This study was to identify the relations among ADL, self-efficacy, physical activity and cognitive function in elders. Methods: A total of 257 subjects aged between 60 and 92 were selected through convenient sampling. Data were collected with a self-reported questionnaire from November 1 to November 30, 2008. Collected data were analyzed with SPSS/WIN 15.0. Results: Differences in ADL, self-efficacy, physical activity, and cognitive functions according to general characteristics were as follows. ADL was significantly different according to age, cohabitation, recognition on health, and successful aging. Self-efficacy was significantly different according to cohabitation, recognition on health, and successful aging. Physical activity was significantly different according to age, educational level, cohabitation, and cognition on health. Cognitive function was significantly different according to age, educational level, job, and recognition on health. The correlation coefficient (r) of the ADL variables was .565 for self-efficacy, .633 for physical activity and .460 for cognitive function. Conclusion: Findings of this study may be useful in understanding the health status of community-dwelling elders and developing more specific health promotion programs.

• Bulletin of the Korean Chemical Society
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• 제35권7호
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• pp.2065-2071
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• 2014

${\beta}$-Secretase (beta-amyloid converting enzyme 1 [BACE1]) is involved in the first and rate-limiting step of ${\beta}$-amyloid ($A{\beta}$) peptides production, which leads to the pathogenesis of Alzheimer's disease(AD). Therefore, inhibition of BACE1 activity has become an efficient approach for the treatment of AD. Ligand-based and docking-based 3D-quantitative structure-activity relationship (3D-QSAR) studies of acyl guanidine analogues were performed with comparative molecular field analysis (CoMFA) and comparative molecular similarity indices analysis (CoMSIA) to obtain insights for designing novel potent BACE1 inhibitors. We obtained highly reliable and predictive CoMSIA models with a cross-validated $q^2$ value of 0.725 and a predictive coefficient $r{^2}_{pred}$ value of 0.956. CoMSIA contour maps showed the structural requirements for potent activity. 3D-QSAR analysis suggested that an acyl guanidine and an amide group in the $R_6$ substituent would be important moieties for potent activity. Moreover, the introduction of small hydrophobic groups in the phenyl ring and hydrogen bond donor groups in 3,5-dichlorophenyl ring could increase biological activity.

• 동서간호학연구지
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• 제14권1호
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• pp.44-55
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• 2008

Purpose: This research concludes the 110 male and female elderlies who are over 60 years old in C welfare center of G city to examine the effect of self-esteem between leisure activity and life satisfaction. Method: The types of participation leisure consist of 20 questions in five different categories. To measure the value of life satisfaction, the scale of life satisfaction for the elderly which is developed by Choi (1986) is used. The self-esteem was measured by the scale developed by Rosenberg (1965) and translated by Jeon (1974). The data were analyzed by SPSS including frequency, ANOVA, Duncan, Pearson correlation coefficient and multiple regression. Result: There was significant relationship among leisure activity and life satisfaction (r= .410, p= .000), leisure activity and self-esteem (r= .432, p= .000) and life satisfaction and self-esteem (r= .765, p= .000). After testing how leisure activity has effect on the self-esteem, it appears to be 17.9% and after adding life satisfaction it increases to be 59.5%. Conclusion: Synthesizing all following results, there were needed to bepersonal and global effort in many ways to satisfy the economical need of the elderly, and more supports onincreasing participation of the elderly in leisure.

• Food Science and Biotechnology
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• 제17권6호
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• pp.1299-1304
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• 2008

The potential antioxidant activities of different fractions from a 70% methanolic (MeOH) extract of Sonchus oleraceus were assayed in vitro. All of the fractions exception of n-hexane showed a strong antioxidant activity, especially the ethyl acetate (EtOAc) fraction, which showed the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity ($IC_{50}=19.25{\mu}g/mL$). The results of hydroxyl radical scavenging activity and a reducing power assay showed concentration dependence, the EtOAc fraction demonstrating a better result than the other fractions at the same concentration in the studies. Additionally, the fractions' total phenolic (TP) contents was measured, phenolic compounds such as tannic acid, p-coumatric acid, quercetin, epicathchin, and kaempferol being detected by high performance liquid chromatography (HPLC). Meanwhile, a regression analysis revealed a moderate-to-high correlation coefficient between the antiradical activity and the TP contents, suggesting that fractions obtained from the 70% MeOH extract of S. oleraceus are of potential use as sources of antioxidant material.

• 산업식품공학
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• 제15권3호
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• pp.221-229
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• 2011

Optimization study of the hot water extraction for enhancing antioxidative activity from Auricularia auricula was performed by Taguchi approach using orthogonal matrix $L_9(3^4)$ method. The correlation between DPPH radical scavenging activity and the components of samples extracted from different extraction conditions were also analyzed. The correlation coefficient between DPPH radical scavenging activity and melanin content of A. auricula were 0.93, indicating 'good correlation'. The optimum extraction conditions was obtained at the extraction time of 1 hr. temperature of $85^{\circ}C$, solid: water ratio of 1: 40(w/v) and frequency of 2 times. Under these conditions, values of maximum DPPH free radical scavenging activity and melanin contents of A. auricula were $67.21{\pm}2.17$ and $52.94{\pm}2.10 mg/g$, respectively. Melanin content of 1.6 times and DPPH free radical scavenging effect of 130% were enhanced by optimization.

• Nutrition Research and Practice
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• 제13권3호
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• pp.196-204
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• 2019

BACKGROUND/OBJECTIVES: Non-alcoholic fatty liver disease (NAFLD) is a common metabolic disease triggered by epigenetic alterations, including lysine acetylation at histone or non-histone proteins, affecting the stability or transcription of lipogenic genes. Although various natural dietary compounds have anti-lipogenic effects, their effects on the acetylation status and lipid metabolism in the liver have not been thoroughly investigated. MATERIALS/METHODS: Following oleic-palmitic acid (OPA)-induced lipid accumulation in HepG2 cells, the acetylation status of histone and non-histone proteins, HAT activity, and mRNA expression of representative lipogenic genes, including $PPAR{\gamma}$, SREBP-1c, ACLY, and FASN, were evaluated. Furthermore, correlations between lipid accumulation and HAT activity for 22 representative natural food extracts (NExs) were evaluated. RESULTS: Non-histone protein acetylation increased following OPA treatment and the acetylation of histones H3K9, H4K8, and H4K16 was accelerated, accompanied by an increase in HAT activity. OPA-induced increases in the mRNA expression of lipogenic genes were down-regulated by C-646, a p300/CBP-specific inhibitor. Finally, we detected a positive correlation between HAT activity and lipid accumulation (Pearson's correlation coefficient = 0.604) using 22 NExs. CONCLUSIONS: Our results suggest that NExs have novel applications as nutraceutical agents with HAT inhibitor activity for the prevention and treatment of NAFLD.

• International Journal of Internet, Broadcasting and Communication
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• 제15권2호
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• pp.210-217
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• 2023

The purpose of this study is to develop a physical activity program for problem drinkers living in side rooms(Jjok-bang) based on the health belief model. In order to develop a physical activity program suitable for and applicable to the characteristics of the problem drinkers, the detailed components of the health belief model and the learning goals for each program were linked. It consists of a total of 10 activities, and in the introductory stage of each program, physical examination and gymnastics were conducted, and in the development stage, 10 physical activities were conducted to help the movement of large and small muscles and concentration. In the final stage of organizing, it consisted of time to express one's emotions through writing and painting and to reflect. The developed program was commissioned by an expert to derive the content validity coefficient (CVI=.75). Through the physical activity program developed in this study, it is believed that the problem drinking behavior of problem drinkers living in side rooms can be gradually reduced. The physical activity program developed in this study is expected to gradually reduce the problems of problem drinkers living in Jjock-bang. In the next step, we propose a study that quantitatively and qualitatively analyzes the intervention effect by applying this program to clients.

• 분석과학
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• 제36권1호
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• pp.44-52
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• 2023

This protocol clarifies a simple and precise method for measuring the activity of xanthine oxidase (XO) enzyme inhibitor. XO enzyme, which accelerates oxidative stress-related disorders through its capacity to generate hydrogen peroxide and superoxide anion radicals (O₂^•-), has been found to be inhibited by several plant extracts. Enzyme samples were incubated with a suitable buffer containing adequate amounts of xanthine as a substrate to determine XO activity. The method depends on direct measurements of uric acid and hydrogen peroxide production to test XO with and without interference. The CUPRAC reagent (Cu(Nc)₂²⁺) was used to inhibit enzyme reaction after incubation was complete. The generated urate and peroxide reduced the Cu(II)-neocuproine complex (Cu(Nc)₂²⁺) to a brightly colored Cu(I)-neocuproine complex (Cu(Nc)₂⁺), which was assessed with a spectrophotometer at 450 nm. XO activity was found to be directly related to the increased absorbance of the colored Cu(I)-neocuproine complex (Cu(Nc)₂⁺). To eliminate catalase enzyme interference, the proposed method used sodium azide and was validated against XO activity using the UV method in matched samples with t-test analysis. The proposed assay can determine XO activity with high precision, as indicated by the correlation coefficient (R² = 0.9935) from comparison with the reference protocol.