• BMB Reports
• /
• v.32 no.5
• /
• pp.486-491
• /
• 1999

An engineered cDNA from Phanerochaete chrysosporium encoding both the mature and propeptide-sequence regions of lignin peroxidase H2 (Lip H2) was overexpressed in Escherichia coli BL21 (DE3) to evaluate its catalytic characteristics and potential application as a pollution scavenger. All expressed proteins were aggregated in an inactive inclusion body, which might be due to inherent disulfide bonds. Active enzyme was obtained by refolding with glutathione-mediated oxidation in refolding solution containing $Ca^{2+}$, heme, and urea. Propeptide-sequence region was not processed as evidenced by N-terminal sequence analysis. Recombinant Lip H2 (rLip H2) had the same physical properties of the native protein but differed in the $K_{cat}$. Catalytic efficiency ($k_{cat}/K_m$) of rLip H2 was slightly higher than that of the native enzyme. In order to express an active protein, fusion systems with thioredoxin or Dsb A, which have disulfide isomerase activity, were used. The fused proteins expressed by the Dsb A fusion vector were aggregated, whereas half of the thioredoxin fusion proteins were recovered as a soluble form but still catalytically inactive. These results suggest that Lip H2 may not be expressed as an active enzyme in Escherichia coli although the activity can be recovered by in vitro refolding.

• Journal of Electrical Engineering and Technology
• /
• v.11 no.2
• /
• pp.405-415
• /
• 2016

This paper presents a model predictive control for shunt active power filters in synchronous reference frame using space vector pulse-width modulation (SVPWM). The three phase load currents are transformed into synchronous rotating reference frame in order to reduce the order of the control system. The proposed current controller calculates reference current command for harmonic current components in synchronous frame. The fundamental load current components are transformed into dc components revealing only the harmonics. The predictive current controller will add robustness and fast compensation to generate commands to the SVPWM which minimizes switching frequency while maintaining fast harmonic compensation. By using the model predictive control, the optimal switching state to be applied to the next sampling time is selected. The filter current contains only the harmonic components, which are the reference compensating currents. In this method the supply current will be equal to the fundamental component of load current and a part of the current at fundamental frequency for losses of the inverter. Mathematical analysis and the feasibility of the suggested approach are verified through simulation results under steady state and transient conditions for non-linear load. The effectiveness of the proposed controller is confirmed through experimental validation.

• Journal of Power Electronics
• /
• v.13 no.5
• /
• pp.861-876
• /
• 2013

Instead of extracting every selected harmonic component, the current reference of selective active power filter (APF) can be also obtained by filtering out the fundamental component from distorted load current for computation efficiency. This type of mixed current reference contains kinds of harmonic components and easily involves noises. In this condition, selective harmonic compensation must be realized by the current controller. With regard that selectivity is the most significant feature of controller, this paper presents specific comparison analysis between two types of resonant controllers: proportional-resonant (PR) controller and vector-resonant (VR) controller. The comparison analysis covers the relations, performances, and stability of both controllers. Analysis results conclude that the poorer selectivity of the PR controller could be relatively improved, but limitations from system stability make the improvement hardly realized. By contrast, the VR controller exhibits excellent selectivity and is more suitable for selective APF with mixed current reference. Experimental results from laboratory prototype validate the reasonability of analysis. And the features of each resonant controller are concluded.

• Journal of Power Electronics
• /
• v.18 no.3
• /
• pp.923-930
• /
• 2018

Based on the application of an integrated dynamic brake (IDB) system that uses a PWM inverter fed-AC motor drive to operate the piston, a new active zero state PWM (AZSPWM) is proposed to improve the stability and reliability of the IDB system by suppressing the conducted electro-magnetic interference (EMI) noise under a wide range of load torque. The new AZSPWM reduces common-mode voltage (CMV) by one-third when compared to that of the conventional space vector PWM (CSVPWM). Although this method slightly increases the output current ripple by reducing the CMV, like the CSVPWM, it can be used within the full range of the load torque. Further, unlike other reduced common-mode voltage (RCMV) PWMs, it does not increase the switching power loss. A theoretical analysis is presented and experiments are performed to demonstrate the effectiveness of this method.

• KIEE International Transaction on Electrical Machinery and Energy Conversion Systems
• /
• v.2B no.3
• /
• pp.133-139
• /
• 2002

In this paper, a prototype of an active auxiliary quasi-resonant DC link (QRDCL) snubber assisted voltage source bidirectional power converter (AC to DC and DC to AC) operating at zero voltage soft-switching (BVS) PWM nlode is presented for a Battery Energy Storage System (BESS). The operating principle of this QRDCL circuit and multifunctional control-based converter system, including PWM inverter mode in which energy flows from the battery bank to the three-phase utility-grid in addition to an active PWM converter mode in which energy flows from the utility-grid to the battery banks are described respectively by the control implementation on the basis of d-q coordinate plane transformation. The multifunctional operation characteristics of this three-phase ZVS PWM bi-directional converter with QRDCL is demonstrated fer a BESS under the power conditioning and processing schemes of energy supply mode and energy storage mode, and compared with a conventional three-phase hard switching PWM bi-directional converter for a BESS. The effectiveness of the three-phase ZVS PWM hi-directional converter with QRDCL is proven via the simulation analysis.

• The Bulletin of The Korean Astronomical Society
• /
• v.41 no.1
• /
• pp.46.1-46.1
• /
• 2016

The formation process of a current sheet is important for solar flare from a viewpoint of a space weather prediction. We therefore derive the temporal development of the spatial and statistical distribution of electric current density distributed in a flare-producing active region to describe the formation of a current sheet. We derive time sequence distribution of electric current density by applying a nonlinear force-free approximation reconstruction to Active Region 12158 that produces an X1.6-class flare. The time sequence maps of photospheric vector magnetic field used for reconstruction are captured by a Helioseismic and Magnetic Imager (HMI) onboard Solar Dynamic Observatory (SDO) on 10th September, 2014. The spatial distribution of electric current density in NLFFF model well reproduce observed sigmoidal structure at the preflare phase, although a layer of high current density shrinks at the postflare phase. A double power-law profile of electric current density is found in statistical analysis. This may be expected to use an indicator of the occurrence of a solar flare.

• Earthquakes and Structures
• /
• v.17 no.2
• /
• pp.131-141
• /
• 2019

Structural control systems have uncertainties in their structural parameters and control devices which by using reliability analysis, uncertainty can be modeled. In this paper, reliability of controlled structures equipped with semi-active Magneto-Rheological (MR) dampers is investigated. For this purpose, at first, the effect of the structural parameters and damper parameters on the reliability of the seismic responses are evaluated. Then, the reliability of MR damper force is considered for expected levels of performance. For sensitivity analysis of the parameters exist in Bouc- Wen model for predicting the damper force, the importance vector is utilized. The improved first-order reliability method (FORM), is used to reliability analysis. As a case study, an 11-story shear building equipped with 3 MR dampers is selected and numerically obtained experimental data of a 1000 kN MR damper is assumed to study the reliability of the MR damper performance for expected levels. The results show that the standard deviation of random variables affects structural reliability as an uncertainty factor. Thus, the effect of uncertainty existed in the structural model parameters on the reliability of the structure is more than the uncertainty in the damper parameters. Also, the reliability analysis of the MR damper performance show that to achieve the highest levels of nominal capacity of the damper, the probability of failure is greatly increased. Furthermore, by using sensitivity analysis, the Bouc-Wen model parameters which have great importance in predicting damper force can be identified.

• Korean Journal of Microbiology
• /
• v.52 no.1
• /
• pp.110-115
• /
• 2016

A small plasmid (pDK4) from the Antarctic marine organism Pseudoalteromonas sp. PAMC 21150, was purified, sequenced and analyzed. pDK4 was determined to be 3,480 bp in length with a G+C content of 41.64% and contains three open reading frames encoding a replication initiation protein (RepA), a conjugative mobilization protein (Mob) and a hypothetical protein. PCR-amplified pDK4 was cloned in high-copy pUC19 to yield the fusion vector pDOC153. The chloramphenicol resistance gene was inserted into pDOC153 to give an ampicillin and chloramphenicol-resistant, Pseudoalteromonas - Escherichia coli shuttle vector (7,216 bp; pDOC155). The TonB-dependent receptor (chi22718_IV ) and exochitinase (chi22718_III ) genes from Arctic marine P. issachenkonii PAMC 22718 were cloned into pDOC155 to produce pDOC158 and pDOC165, respectively. Both vector derivatives were transferred into plasmid-free Pseudoalteromonas sp. PAMC 22137 by the triparental mating method. PCR experiments showed that the genes were stably maintained both in Pseudoalteromonas sp. PAMC 22137 and E. coli $DH5{\alpha}$ cells, indicating the potential use of pDOC155 as a new gene transfer system into marine Pseudoalteromonas spp.

• Journal of Korean Association for Spatial Structures
• /
• v.8 no.4
• /
• pp.73-80
• /
• 2008

The objective of current study is to develop an optimization technique for the seismic actively controlled building structures using active tendon devices by an efficient solution of LQR control gain. In order to solve the active control system, the Ricatti closed-loop algorithm has been applied, and the state vector has been formulated by the transfer matrix and solved by a numerical technique of the trapezoidal rule. The time-delay problem has been also considered by phase compensation. To optimize the performance index, the ratio of the weighted matrix is the design variable, allowable story drift limits of IBC 2000 and tendon forces have been applied as restraint conditions, and the optimum control program has been developed with the algorithm of the SUMT technique. In examples of the optimization problem of eight stories shear buildings, it is evaluated that the optimum controlled building is more suitable in the control of earthquake response than the uncontrolled system and can reduce the performance index to compare with the controlled system with a constant ratio of the weighted matrix.

• Journal of Power Electronics
• /
• v.11 no.3
• /
• pp.360-368
• /
• 2011

This paper proposes an active frequency with a positive feedback in the d-q frame anti-islanding method suitable for a robust phase-locked loop (PLL) algorithm using the FFT concept. In general, PLL algorithms for grid-connected PV PCS use d-q transformation and controllers to make zero an imaginary part of the transformed voltage vector. In a real grid system, the grid voltage is not ideal. It may be unbalanced, noisy and have many harmonics. For these reasons, the d-q transformed components do not have a pure DC component. The controller tuning of a PLL algorithm is difficult. The proposed PLL algorithm using the FFT concept can use the strong noise cancelation characteristics of a FFT algorithm without a PI controller. Therefore, the proposed PLL algorithm has no gain-tuning of a PI controller, and it is hardly influenced by voltage drops, phase step changes and harmonics. Islanding prediction is a necessary feature of inverter-based photovoltaic (PV) systems in order to meet the stringent standard requirements for interconnection with an electrical grid. Both passive and active anti-islanding methods exist. Typically, active methods modify a given parameter, which also affects the shape and quality of the grid injected current. In this paper, the active anti-islanding algorithm for a grid-connected PV PCS uses positive feedback control in the d-q frame. The proposed PLL and anti-islanding algorithm are implemented for a 250kW PV PCS. This system has four DC/DC converters each with a 25kW power rating. This is only one-third of the total system power. The experimental results show that the proposed PLL, anti-islanding method and topology demonstrate good performance in a 250kW PV PCS.