• Journal of Microbiology and Biotechnology
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• 제25권2호
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• pp.196-205
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• 2015

A Sulfolobus-E. coli shuttle vector for an efficient expression of the target gene in S. acidocaldarius strain was constructed. The plasmid-based vector pSM21 and its derivative pSM21N were generated based on the pUC18 and Sulfolobus cryptic plasmid pRN1. They carried the S. solfataricus P2 pyrEF gene for the selection marker, a multiple cloning site (MCS) with C-terminal histidine tag, and a constitutive promoter of the S. acidocaldarius gdhA gene for strong expression of the target gene, as well as the pBR322 origin and ampicillin-resistant gene for E. coli propagation. The advantage of pSM21 over other Sulfolobus shuttle vectors is that it contains a MCS and a histidine tag for the simple and easy cloning of a target gene as well as one-step purification by histidine affinity chromatography. For successful expression of the foreign genes, two genes from archaeal origins (PH0193 and Ta0298) were cloned into pSM21N and the functional expression was examined by enzyme activity assay. The recombinant PH0193 was successfully expressed under the control of the gdhA promoter and purified from the cultures by His-tag affinity chromatography. The yield was approximately 1 mg of protein per liter of cultures. The enzyme activity measurements of PH0913 and Ta0298 revealed that both proteins were expressed as an active form in S. acidocaldarius. These results indicate that the pSM21N shuttle vector can be used for the functional expression of foreign archaeal genes that form insoluble aggregates in the E. coli system.

• 한국전자파학회논문지
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• 제29권11호
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• pp.868-877
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• 2018

본 논문에서는 SVR(Support Vector Regression)을 이용한 컨포멀 배열 안테나의 빔 형성 알고리즘을 제안한다. 기존의 최소자승법 기반 알고리즘은 모든 샘플의 오차를 고려하는 반면에, SVR은 정해진 오차 한계를 벗어나는 샘플들을 통해 가중치를 결정하여 희소(sparse)한 해를 가지며 과적합(over-fitting) 문제를 최소화하는 장점을 갖고 있다. 제안된 알고리즘의 성능을 검증하기 위해 실험적으로 측정된 컨포멀 배열 안테나 능동 소자 패턴을 SVR에 적용하여 목적 빔 배턴으로 근사시키는 가중치를 구하였으며, SVR로 얻은 가중치와 최소자승법을 통해 얻은 가중치를 실측한 소자패턴에 적용하여 빔 형성 성능을 비교하였다.

• 한국정보전자통신기술학회논문지
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• 제17권4호
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• pp.229-235
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• 2024

최근 몇 년 동안 인공지능과 기계 학습 분야에서 대량의 데이터를 효율적으로 처리하고 해석하는 방법에 대한 연구가 활발히 진행되고 있다. 이러한 데이터 처리 기술 중 하나인 벡터 기호 아키텍처(Vector Symbolic Architecture, VSA)는 고차원 벡터를 이용하여 복잡한 기호와 데이터를 표현하는 혁신적인 접근법을 제시한다. VSA는 특히 자연어 처리, 이미지 인식, 로봇 공학 등 다양한 응용 분야에서 주목받고 있다. 본 연구는 VSA 방법론들의 특성과 성능을 정량적으로 평가하기 MNIST 데이터셋에 5가지 VSA 방법론을 적용하여 인코딩 속도, 디코딩 속도, 메모리 사용량, 복원 정확도와 같은 주요 성능 지표를 벡터 길이별로 측정하였다. 인코딩 속도와 디코딩 속도에서 BSC와 VT가 상대적으로 빠른 성능을 보였으며, MAP과 HRR은 상대적으로 느렸다. 메모리 사용량에서는 BSC가 가장 효율적이었고, MAP이 가장 많은 메모리를 사용하였다. 복원 정확도는 MAP이 가장 높았으며, BSC가 가장 낮았으며 연구 결과는 적용 영역에 따라 적절한 VSA 방법론을 선택할 수 있는 기준을 제시할 수 있다.

• Journal of Microbiology and Biotechnology
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• 제3권1호
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• pp.6-11
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• 1993

An autonomously replicating sequence (Kf-ARS1) of Kluyveromyces fragilis was cloned from the genomic library which was constructed using pHN134 as a cloning vector to make a new host-vector system for the production of heterologous protein from K. fragilis as a host. The cloning vector pHN134 was composed of $Km^r, Ap^r$ and multiple cloning site in LacZ . A clone carrying Kf-ARS1 was isolated and the recombinant plasmid was designated as pIKD102. The cloned fragment was 2.3 kb (EcoRI/EcoRI) in length. Subcloning experiment showed that the region for ARS activity was 1.5 kb (SalI/EcoRI) fragment. It was shown that the Kf-ARS1 was active in Saccharomyces cerevisiae and Kluyveromyces fragilis.

• Journal of Microbiology and Biotechnology
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• 제2권4호
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• pp.237-242
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• 1992

In order to clone the gene coding for alkaline phosphatase in the yeast Kluyveromyces fragilis, a genomic library was constructed using the yeast-E. coli shuttle vector pHN114 as a cloning vector. From the genomic library, a clone carrying the gene was isolated and the plasmid was designated as pSKH101. A restriction enzyme map was made using this plasmid. Subcloning experiments and complementation studies showed that alkaline phosphatase was active only in the original 3.1 kb insert. Southern hybridization analysis confirmed that the cloned DNA fragment was derived from K. fragilis genomic DNA. Using a minicell experiment, the product of the cloned gene was identified as a protein with a molecular weight of 63 KDa. A 0.6 kb HindIII fragment, which showed promoter activity, was isolated using the E. coli promoter-probe vector pKO-1.

• Journal of Microbiology and Biotechnology
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• 제8권1호
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• pp.34-41
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• 1998

A method was developed for the controlled expression of an antimicrobial peptide magainin in Escherichia coli. A series of concatemeric magainin genes was constructed with a gene amplification vector, and fused to the 3'end of malE gene encoding the affinity ligand, E. coli maltose-binding protein (MBP). The construct directed the synthesis of the fusion protein with the magainin polypeptide fused to the C-terminus of MBP. The fusion protein was expressed in a tightly regulatable expression system which was under the control of an invertible promoter. The MBP-fused magainin monomer was expressed efficiently. However, the expression level of the MBP-fused magainin in E. coli decreased with the increasing size of multimers possibly because of the transcription and translation inhibition by the multimeric peptides. After purification using an amylose affinity column, the fusion protein was digested by factor Xa at a specific cleavage site between the monomers. The recombinant magainin had an antimicrobial activity identical to that of synthetic magainin. This experiment shows that a biologically active, antimicrobial peptide magainin can be produced by fusing to MBP, along with a promoter inversion vector system.

• Transactions on Control, Automation and Systems Engineering
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• 제3권4호
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• pp.217-222
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• 2001

We propose a new two-degree of freedom parallel mechanism for a haptic device and will refer to the mechanism as the SenSation. The SenSation is designed in order to improve the kinematic performanced and to achieve static balance. We use the panto graph mechanisms in order to change the location of active joints, which leads to transform a direct kinematic singularity into a nonsingularity. The direct kinematic singular configurations of the SenSation occur near the workspace boundary. Using the property that position vector of rigid body rotating about a fixed point is normal to the velocity vector, Jacobian matrix is derived. Using the vector method, two different types of singularities of the SenSation can be identified and we discuss the physical significance of each of the three types of singularities. We will compare the kinematic performances(force manipulability ellipsoid, kinematic isotropy) of the SenSation with those of five-var parallel mechanism. By specifying that the potential energy be fixed, the conditions for the static balancing of the SenSation is derived. The static balancing is accomplished by changing the center of mass of the links.

• 대한전자공학회논문지
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• 제9권1호
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• pp.17-24
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• 1972

단순한 외부 측정만으로써 수동 및 능동 회로망에서의 한개의 결함요소(Faulty Element)를 결정하기위해서 벡터를 이용한 한 방법을 연구했다. 큰 회로망은 몇개의 작은 회로망이 연결되었다고 생각할 수 있다. 여러 주파수에서의 전달 함수의 크기와 회로요소의 변화와의 사이에서 일어나는 관계를 이용함으로써 Fault Simulation Curve를 그릴 수 있다. 이곡선으로부터 결함식별영역(Fault Identification Region)을 정의한다. 정의된 결함식별영역에 따라 결함시별 매트릭스(Fault Irlentification Matrix)가 형성된다. 어떤 회로로부터 측정된 치를 성분으로하는 벡터를 결함식별 매트릭스에 곱해줌으로써 다른 한개의 벡터를 유도하는데 이유도된 벡터의 성분이 회로의 결함요소를 식별해주는 것이다. 결함식별방법을 위한 한 시험절차가 계시되고 실험에 의하여 입증되었다.

• Earthquakes and Structures
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• 제18권4호
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• pp.493-506
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• 2020

In this paper, a new opposition based charged system search (CSS) is proposed to be used as a parameter identification of highly nonlinear semi-active magneto-rheological damper. By replacing the opposition particles with current solutions, the mentioned strategy is used to enhance the search space and to increase the exploration of CSS. To investigate the effectiveness of the proposed method, a nonlinear modified Bouc-Wen model of MR damper is considered to find its parameters, and compare it with those achieved from experimental model of MR damper. Also, by exploiting the sensitivity analysis and using the importance vector, the less importance parameters in the Bouc-Wen model are eliminated which makes the MR damper model simpler. Results demonstrate the new proposed algorithm (OBLCSS) has a high ability to tackle highly nonlinear problems. Based on the results of the α importance vector, a simplified model is proposed and its parameters are identified by using the presented OBLCSS algorithm. The simplified proposed model also has a high capability of estimating damper responses.

• Journal of Microbiology and Biotechnology
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• 제23권6호
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• pp.850-855
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• 2013

Finding new antimicrobial activities by functional metagenomics has been shown to depend on the heterologous host used to express the foreign DNA. Therefore, efforts are devoted to developing new tools for constructing metagenomic libraries in shuttle vectors replicatable in phylogenetically distinct hosts. Here we evaluated the use of the Escherichia coli-Bacillus subtilis shuttle vector pHT01 to construct a forest-soil metagenomic library. This library was screened in both hosts for antimicrobial activities against four opportunistic bacteria: Proteus vulgaris, Bacillus cereus, Staphylococcus epidermidis, and Micrococcus luteus. A new antibacterial activity against B. cereus was found upon screening in B. subtilis. The new antimicrobial agent, sensitive to proteinase K, was not active when the corresponding DNA fragment was expressed in E. coli. Our results validate the use of pHT01 as a shuttle vector and B. subtilis as a host to isolate new activities by functional metagenomics.