• Bulletin of the Korean Chemical Society
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• v.28 no.2
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• pp.271-275
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• 2007

γ -Glutamyl transpeptidase (EC 2.3.2.2) plays a key role in glutathione metabolism by catalyzing the transfer of the γ -glutamyl residue and hydrolysis of glutathione. The functional residues at the active site of the rat kidney γ -glutamyl transpeptidase were investigated by kinetic studies at various pH, the treatment of diethylpyrocarbonate (DEPC), and photooxidation in presence of methylene blue. An ionizable group affecting the enzymatic activity with an apparent pKa value of 7.1, which is in the range of pKa values for a histidine residue in protein, was obtained by examining the pH-dependence of kinetic parameters. The pH effect on the photoinduced inactivation rate of the enzyme corresponds to that expected for the photooxidation of the free histidine. The involvement of a histidine in the catalytic site of the enzyme was further supported by DEPC modification accompanied by an increase in absorbance at 240 nm, indicating the formation of Ncarbethoxyhistidine. The histidine located at the position of 382 in the precursor of the enzyme is primarily suspected based on the amino acid sequence alignment of the transpeptidases from various organisms.

• Structural Engineering and Mechanics
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• v.42 no.1
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• pp.73-93
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• 2012

With an active structural involvement in spiral case structure (SCS) that is always the design and research focus of hydroelectric power plant (HPP), the compressible membrane sandwiched between steel spiral case and surrounding reinforced concrete was often assumed to be linear elastic material in conventional design analysis of SCS. Unfortunately considerable previous studies have proved that the foam material serving as membrane exhibits essentially nonlinear mechanical behavior. In order to clarify the effect of membrane (foam) material's nonlinear stress-strain behavior on SCS, this work performed a case study on SCS with a compressible membrane using the ABAQUS code after a sound calibration of the employed constitutive model describing foam material. In view of the successful capture of fitted stress-strain curve of test by the FEM program, we recommend an application and dissemination of the simulation technique employed in this work for membrane material description to structural designers of SCS. Even more important, the case study argues that taking into account the nonlinear stress-strain response of membrane material in loading process is definitely essential. However, we hold it unnecessary to consider the membrane material's hysteresis and additionally, employment of nonlinear elastic model for membrane material description is adequate to the structural design of SCS. Understanding and accepting these concepts will help to analyze and predict the structural performance of SCS more accurately in design effort.

• Journal of the Korean Society of Food Science and Nutrition
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• v.24 no.4
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• pp.570-577
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• 1995

Aspergillus sp. SB-2704 was selected for its strong polygalacturonase activity among various strain of mold found in soil. It was found that production of polygalacturonase reached to maximum when the wheat bran medium containing 1% polypepton, 1% glucose, and 0.2% FeSO4 were cultured for 3 days at 35$^{\circ}C$. Polygalacturonase was purified 20.90 fold from Aspergillus SB-2704. The purification procedures include ammonium sulfate treatment, gel filtration on Sephdex G-150 and DEAE-cellulose ion exchange chromatography. Yield of the enzyme purification was 4.34%. Purified enzyme was confirmed as a single band by the polyacrylamide gel electrophoresis. When the purified enzyme was applied to SDS-polyacrylamide gel electrophoresis, the molecular weight was estimated to be 36,000. The optimum pH for the enzyme activity was 5.5 and optimum temperature was 5$0^{\circ}C$. The enzyme is stable in acidic condition. The activity of purified enzyme was inhibited by Pb2+, Hg2+ and Ba2+, whereas activated by Cu2+, Mn2+, Mg2+ and Fe2+. The activity of polygalacturonase was inhibited by the treament wit maleic anhydride, iodine, and EDTA. The result indicate the possible involvement of histidine and metal ion at active site.

• Journal of Microbiology and Biotechnology
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• v.11 no.5
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• pp.838-844
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• 2001

The enzyme Fuc aldolase from Methanococcus jannaschii that catalyzes the aldol condensation of DHAP and L-lactaldehyde to give fuculose-1-phosphate was inactivated by DEP. The inactivation was pseudo first-order in the enzyme and DEP, which was biphasic. A pseudo second-order rate constant of 120$M^{-1}min^{-1}$ was obtained at pH 6.0 and $25{\circ}C$. Quantifying the increase in absorbance at 240nm showed that four histidine residues per subunit were modified during the nearly complete inactivation. The statistical analysis and the time course of the modification suggested that two or three histidine residues were essential for activity. The rate of inactivation was dependent on the pH, and the pH inactivation data implied the involvement of the amino acid residue with a $pK_a$ value of 5.7. Fuc aldolase was protected against DEP inactivation by DHAP, indicating that the histidine residues were located at the active site of Fuc aldolase. DL-Glyceraldehyde, as an alternative substrate to L-lactaldehyde, showed no specific protection for the Fuc aldolase.

• Journal of Korean Society of Rural Planning
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• v.15 no.3
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• pp.23-32
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• 2009

The purpose of this research was to evaluate rural housing environment through evaluation of the two parties, public officials, the interest group and residents, the beneficiaries surveying general understanding and measuring difference in understanding between public officials and residents. Through the difference in understanding, this study was to propose effective promotion method and desirable direction of rural housing environment. For progress hereafter, by seeking active involvement of residents, this study proposed possible renewal plan of residential environment. Process of this study was first, research documents to examine several rural housing environment related promotions, and several analysis techniques like principal component analysis, multiple regression analysis used to understand different ideas Opinion of Public Officials and Inhabitants. Then take surveys of residents living in renewal zone, and surveys of public officials of borough, which already performed renewal projects to evaluate comprehensively as either residents' or administrators' view.

• Journal of Korean Society of Rural Planning
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• v.25 no.1
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• pp.1-10
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• 2019

This purpose of this study was to investigate the perception and participation satisfaction of residents who participated in the rural landscape improvement project in Geumsan. The projects were conducted in three villages of Geumsan in 2018 and total 96 residents had participated. The results of the questionnaire survey on the participants showed that they usually have interests in community activities and actively participated. As a result of analyzing factors related to the change of residents' perceptions and satisfaction. It was found that there was significant difference in perception and satisfaction according to frequency of participation, not demographic factors. It is interesting that active interaction among residents during the projects and satisfaction on process are important factor for overall project satisfaction. For successful rural landscape improvement projects, it is necessary to encourage and support interaction and communication among residents. In addition, more various programs need to be developed to foster residents' attachment and sense of community.

• The Mathematical Education
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• v.57 no.4
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• pp.413-431
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• 2018

In this study, I developed an activity oriented lesson to support the understanding of probabilistic and quantitative estimating population ratios according to the standard statistical principles and discussed its implications in didactical respects. The developed activity lesson, as an efficient physical simulation activity by sampling with replacement, simulates unknown populations and real problem situations through completely closed 'Closed Box' in which we can not see nor take out the inside balls, and provides teaching and learning devices which highlight the representativeness of sample ratios and the sampling variability. I applied this activity lesson to the gifted students who did not learn estimating population ratios and collected the research data such as the activity sheets and recording and transcribing data of students' presenting, and analyzed them by Qualitative Content Analysis. As a result of an application, this activity lesson was effective in recognizing and reflecting on the representativeness of sample ratios and recognizing the random sampling variability. On the other hand, in order to show the sampling variability clearer, I discussed appropriately increasing the total number of the inside balls put in 'Closed Box' and the active involvement of the teachers to make students pay attention to controlling possible selection bias in sampling processes.

• Bulletin of the Korean Chemical Society
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• v.8 no.4
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• pp.280-285
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• 1987

FMN-dependent glycolate oxidase from spinach is inactivated by diethyl pyrocarbonate at pH 7.0. Inactivation of both apo- and holoenzyme by diethyl pyrocarbonate follows pseudo-first-order kinetics and first order with respect to the reagent. A series of difference spectra of inactivated and native enzymes show a single peak at 240 nm, indicating the modification of histidyl residues. No decrease in absorbance at around 280 nm due to formation of O-carbethoxytyrosine is observed. The rate of inactivation is dependent on pH, and the data for pH dependent rates implicate the involvement of a group with a pKa of 6.9. The activity lost by treatment with diethyl pyrocarbonate could be almost fully restored by incubation with 0.75M hydroxylamine. The reactivation by hydroxylamine and the pH dependence of inactivation are also consistent with that the inactivation is due to modification of histidyl residues. Although coenzyme FMN is without protective effect, the substrate glycolate, the product glyoxylate, and two competitive inhibitors, oxalate and oxalacetate, provide marked protection against the inactivation of the holoenzyme. These results suggest that the inactivation of the oxidase by diethyl pyrocarbonate occurs by modification of essential histidyl residue(s) at the active site.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.19 no.1
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• pp.29-38
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• 2021

The article summarises the status and competence of UJV Rez, a. s. (up to 2012, the Nuclear Research Institute Rez, Czech Republic) in the field of radioactive waste (RAW) management as a company managing of 95% of institutional radioactive wastes in Czech Republic. UJV Rez a. s. has been one of the Czech Republic's key research and engineering institutions in the field of nuclear energy production since 1955. The company processes and conditions prior to storage 95% of so-called institutional RAW and is the principal partner of the state with respect to the research support of the Czech deep geological repository development project. UJV Rez a. s. boasts its own accredited radiochemical analytical test laboratory, unique of its kind in the Czech Republic. Of equal importance is UJV Rez's active participation in a range of international organisations and associations and its involvement in wide range of international projects, and so as European projects. One of them is EU funded project PREDIS: Pre-disposal management of radioactive wastes, that has started at September 2020, focused on the field of low level radioactive waste (LLW) and intermediate level radioactive waste (ILW) pre-disposal.

• Human Ecology Research
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• v.60 no.1
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• pp.21-38
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• 2022

This study uses text mining compares and contrasts consumers' social media discourses on dietary related zero-waste movement before and after COVID-19. The results indicate that the amount of buzz on social networks for the zero- waste movement has been increasing after COVID-19. Additionally, the results of frequency analysis and topic modeling revealed that subjects associated with zero-waste movement were more diversified after COVID-19. Although the results of a sentiment analysis and word cloud visualization confirmed that consumers' positive responses toward the zero-waste have been increasing, they also revealed a need to educate and encourage those who are still not aware of the need for zero-waste. Finally, consumers mentioned only a small number of companies participating in zero-waste movement on SNS, indicating that the level of active involvement by such companies is much lower than that of consumers. Theoretical and educational implications as well as those for government policy-making are considered.