• Journal of Applied Biological Chemistry
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• 제60권3호
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• pp.265-269
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• 2017

Environment-friendly, commercially-available agricultural products were investigated for antimicrobial activity against Sclerotinia sclerotiorum, as a pathogen of sclerotium disease. Then ${\beta}$-thujaplicin from Chamaecyparis obtuse was investigated for antifungal activity against six kinds of pathogenic fungi. It showed a statistically significant (p <0.001) growth inhibition effect on Sclerotinia sclerotiorum as a pathogen of sclerotium disease, Rhizoctonia solani AG-4 as a pathogen of damping off, Phytophthora capsici as a pathogen of phytophthora blight, and Colletotrichum coccodes as a pathogen of anthracnose at a concentration of 50 ppm and on Stemphylium solani as a pathogen of spotting disease and Alternaria alternata as a pathogen of black mold at a concentration of 100 ppm. In conclusion, these results indicate that it may be possible to develop environment-friendly agricultural products using ${\beta}$-thujaplicin compounds.

• 생약학회지
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• 제26권2호
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• pp.122-129
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• 1995

The study on phytochemical analysis and the biological activity of Kalopanacis Cortex was carried out in this research. As a phytochemical result, liriodendrin as a lignan glycoside was isolated and characterized. Two subfraction separated from the acidic substance of $CHCl_3$ fraction were saturated and unsaturated fatty acid, respectively. Saturated fatty acid mixture identified from GC-MS tool was as follows: palmitic acid, stearic acid, arachidic acid, heneicosanoic acid, docosanoic acid, tricosanoic acid, tetracosanoic acid, pentacosanoic acid, hexacosanoic acid and octacosanoic acid. Unsaturated fatty acid was found to be linoleic acid on the basis of spectroscopic method. An active principle of liriodendrin exhibited significant antihepatotoxic activity but failed to show a considerable antiedemic activity. In this paper, the result of writhing test on liriodendrin was also described.

• Journal of Pharmaceutical Investigation
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• 제36권4호
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• pp.245-251
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• 2006

The novel microsphere blending and multiple emulsion method by single process was tried to prepare sustained release microspheres which release a physiologically active substance for long periods of time. A drug was separately dissolved in each of two or more oils containing biodegradable polymers to give the primary oil phases. The primary oil phases were dispersed in single aqueous phase in succession. From the drug-dispersed solution, the organic solvent was removed to produce microspheres. The accelerated drug release from the microsphere formulation prepared by single process through the multiple emulsion method was very similar to a physical blending of separately prepared microspheres using the same polymers. But long term release was not same. In this study, leuprorelin acetate loaded poly(lactide-co-glycolide) microsphere formulation for one-month delivery was developed by the multi-emulsion method followed by solvent extraction/evaporation method.

• Applied Microscopy
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• 제46권1호
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• pp.20-26
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• 2016

Our work on $Sc_2O_3-W$ matrix dispenser cathodes had been reviewed in this paper. The cathode with uniform distribution of $Sc_2O_3$ had been obtained using liquid-liquid doping method. The cathode had excellent emission property, i.e., the emission current density in pulse condition could reach over $35A/cm^2$. It was found that the cathode surface was covered by a Ba-Sc-O active substance multilayer with a thickness of about 100 nm, which was different from the monolayer and semiconducting layer in thickness. Furthermore, the observation results displayed that nanoparticles appeared at the growth steps and the surface of tungsten grains of the fully activated cathode. The calculation result indicated that the nanoparticles could cause the increase of local electric field strengths. We proposed the emission model that both the Ba-Sc-O multilayer and the nanoparticles distributing mainly on the growth steps of the W grains contributed to the emission. The future work on this cathode has been discussed.

• 한국식품과학회지
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• 제27권5호
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• pp.807-812
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• 1995

두릅나무(Aralia elata $S_{EEMANN}$) 껍질의 MeOH 추출물이 세균과 효모, 곰팡이에 대해 항미생물 활성을 보여 solvent fractionation, silica gel adsorption chromatography, Sephadex LH-20 column chromatography, silica gel partition chromatography, HPLC, TLC 등의 기법으로 주활성본체를 분리하였다. MS, GC-MS, IR, 1^H-NMR, 13^C-NMR 등의 기기분석을 통해 항미생물 활성의 본체구명을 시도한 결과 3,4-dihydroxybenzoic acid로 동정되었다. 3,4-dihydroxybenzoic acid가 항미생물 활성본체로서 분리된 것도 처음으로 생각되며 두릅에서 이 물질이 분리, 동정된 것도 처음으로 생각된다. 건조 두릅껍질 g당 3,4-dihydroxybenzoic acid의 함량은 0.869mg 정도이었다.

• 대한수의학회지
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• 제46권2호
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• pp.87-96
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• 2006

We established an in vitro experimental system using the following procedure. We first introduced tritium-labeled norepinephrine ([$^3$H]-NE) into PC12 cells, The [$^3$H]-NE incorporated into PC12 cells were then stimulated by a high concentration (60 mM) of $K^+$ buffer during 12 minutes. Then, we collected $100{\mu}l$ supernatant and counted the amount of [$^3$H]-NE release from PC12 cells with a scintillation counter. After screening fungal, Streptomyces spp. or bacterial product using this experimental sytem, we obtained FS11052 from Streptomyces spp. which inhibited [$^3$H]-NE release from PC12 cells. FS11052 also inhibits the release of ATP as a neurotransmitter of PC12 cells and rat cortical neurons, The inhibitory effect was seen even when the PC12 cells were treated with low $K^-$ buffer containing ionomycin ($1{\mu}M$) as an ionopore. This result suggests that the inhibitory action of FS11052 on neurotransmitter release appeared after the influx of $Ca^{2+}$.

• 농약과학회지
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• 제11권2호
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• pp.82-86
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• 2007

포도나무 잎의 메탄올 추출물을 n-hexane, ethylacetate, n-butanol $H_2O$으로 순차적으로 용매분획 하였다. Ethylacetate 분획으로부터 silica gel chromatography와 재결정하여 활성물질을 분리 정제하였다. 이 화합물의 화학구조를 EI-MS의 조각 데이터를 근거로 분석한 결과 betulin으로 구조결정 되었다. 이 화합물이 고추 역병균(Phytophthora capsici), 고추 탄저병균(Colletotrichum acutatum)에 대한 항균활성(in vitro)을 측정한 결과, 100 ${\mu}g\;mL^{-1}$에서 각각 52.1%, 40.8%의 균사생육 억제효과를 보였으나, 잔디 마름병(Rhizoctonia solani) 균의 균사생육 억제 효과는 나타나지 않았다.

• Archives of Pharmacal Research
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• 제29권2호
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• pp.140-144
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• 2006

Four xanthones were isolated from mycelia of Emericella variecolor, an endophytic fungus isolated from the leaves of Croton oblongifolius. Their structures were elucidated by spectroscopic analysis to be shamixanthone, 14-methoxytajixanthone-25-acetate, tajixanthone methanoate, and tajixanthone hydrate. All compounds were tested for cytotoxic activity against various human tumor cell lines including gastric carcinoma, colon carcinoma, breast carcinoma, human hepatocarcinoma, and lung carcinoma. The antitumor activities of these xanthones were compared with that of doxorubicin hydrochloride, a chemotherapeutic substance. All of them showed moderate activities and were selective against gastric carcinoma, colon carcinoma, and breast carcinoma. Only tajixanthone hydrate exhibited moderate activity against all cancer cell lines. Furthermore, under the test conditions it was found that 14-methoxytajixanthone-25-acetate and tajixanthone hydrate are almost as active as doxorubicin hydrochloride against gastric carcinoma (KATO3) and breast carcinoma (BT474).

• Current Research on Agriculture and Life Sciences
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• 제20권
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• pp.25-31
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• 2002

1. 꽃무릇으로부터 용매를 다르게 하여 잎과 인경의 MeOH와 수용추출물을 bioassay 한 결과, 농도가 5% 및 10%로 증가하였을 때는 전혀 발아되지 않았으며, 1%에서는 근장이 크게 억제되었다. 용매간의 추출효과에는 차이가 없었으나, 상추의 생장에서는 인경추출액이 잎추출액보다 억제효과가 다소 높은 것으로 보였다. 2. 꽃무릇의 인경을 추출하여 UV를 조사하였을 때 Rf 0.49-0.52에서 보인 spot는 GC-MS에 의해 lycoris alkaloid계통의 lycorenan으로 동정되었다.

• 한국양봉학회지
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• 제32권4호
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• pp.391-394
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• 2017

Propolis is made by bees collecting protective material or essence of plants and mixing with saliva and enzymes produced by the salivary glands. It is used to repair the inside of the honeycomb, keep it sterile, and adjust the temperature and humidity. Propolis is a natural antibiotic substance that it is used to make a clean room by coating the cell before the queen bee lay eggs, and preventing the bacteria from invading by using with wax when sealing the nursery room. Propolis extract is a health functional food with antioxidant and oral antimicrobial effects. In order to use propolis in food, its active ingredients are extracted with ethanol. Water-soluble propolis was prepared by mixing and stirring honey and ethanol extracted propolis (EEP) solution. When 1kg of honey and 100ml of ethanol extracted propolis solution were mixed and stirred, the total flavonoid content of water-soluble propolis was $6.6{\pm}1.1mg/10g$, and the free radical scavenging effects of water-soluble propolis were 54 to 74%.