• Reproductive and Developmental Biology
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• 제31권2호
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• pp.77-82
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• 2007

This study was conducted to investigate the effects of oocyte maturational age and activation condition on in vitro development of porcine parthenogenetic embryos (parthenotes). Porcine follicular oocytes were matured in vitro for 30 to 44 hr. Maturation rate was examined during in vitro maturation (IVM) every 2 hr interval. The cdc2 kinase activity was measured at 36 and 44 hr of IVM. Some oocytes were activated at 36 or 44 hr of IVM by three different conditions; 1) single electric stimulation (1.5 kV/cm for $30{\mu}sec$; ES), 2) double electric stimulations (1.5 kV/cm for $30{\mu}sec$, followed by 1.0 kV/cm for $50{\mu}sec$ after 1 hr; ES+ES) or 3) ES+ES followed by culture in 6-dimethlyaminopurine (6-DMAP) for 4 hr (ES+ES+D), and cultured for 6-7 days. Maturation rate was significantly increased as culture period was increased to 36 hr (66.9%, p<0.05), and then gradually increased to 87.1% at 44 hr of IVM. The cdc2 kinase activity was decreased (p<0.05) with culture period prolonged from 36 hr to 44 hr. Lower blastocyst formation rate (4.3%, p<0.05) were obtained by ES in 36 hr-matured oocytes compared to other treatments (16.5 and 20.5%) in the same age and the same treatment in 44 hr-matured oocytes (15.0%). High blastocyst formation rate (23.6%) was obtained by ES+ES+D in 44 hr-matured oocytes (p<0.05). These results demonstrate that porcine oocyte activation and in vitro development of parthenotes can be affected by interactions between oocyte maturational age and activation condition.

• 한국가축번식학회지
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• 제18권3호
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• pp.217-228
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• 1994

These experiments were carried out to establish the optimal condition of electrostimulatin inducing cell fusion and oocyte activation for nuclear transplantation in mouse embryos. Eggs selected for cell fusion or activation by electrostimulation were equilibrated for 5~10 min. in 0.3M sucrose solution and electrostimulated for 60$\mu$sec using 1 pulse of 60, 70, 80, 90 or 100 volts DC with electrodes 0.2 mm apart. Then they were cultured in 20${mu}ell$ dropsof Tyrode's solution. The results of these experiments are as follows : 1. When one pulse of 60, 70, 80, 90 or 100 volts DC for 60$\mu$sec were applied to 2-cell embryos for fusion of blastomeres, fusion rates were 50.0, 81.7, 91.7, 100 and 100%, respectively ; and developmental rates of fused embryos to blastocyst were 76.7 to 81.5%. Higher fusion rates were observed in 90V and 100V. 2. The average cell number in fused embryos developed to blastocyst was about half of the cell number in diploid controls; and the cell number decreased with increasing of voltages. 3. When pulse numbers were increased, fusion rates improved, but developmental rates were not signficiantly different from the group for which the number of pulse was not increased. And the cell number of blastocyst decreased even more. 4. Oocytes aged for 6hrs after ovulation were electrostimulated for oocyte activation by the same method used for cell fusion. Rates of oocyte activated by electrostimulation were 45.3 to 60.4%, and fragmentation rates were 7.5~15.1%. The lysis rates were 17.0~34.0%. The results of these experiments indicate that the optimal condition for achieving cell fusion and activation is 1 pulse, duration 60$\mu$sec in 90 Volt. The results also show that this condition is suitable for nuclear transplantation using mouse eggs.

• Reproductive and Developmental Biology
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• 제30권2호
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• pp.115-118
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• 2006

Cyclin B1 is known to reflect the M-phase promoting factor (MPF), a universal regulator of G2/M-phase transition, activity during the process of oocytes maturation. To investigate whether culture condition affects the maturation rate and the expression of cyclin B1 protein, bovine immature oocytes are stimulated and cultured according to the following protocols: Experiment 1: denuded oocytes (denude) only, COC only, denuded oocytes+granulosa cells (denude+GCs) and COC+GCs; Experiment 2: no-activation (control), 7% ethanol for 5 min and $10{\mu}l/ml$ ionomycin for 5 min at immediately before maturation. The maturation rates of denude and no-activation group were significantly lower in both experiments (P<0.05), respectively. Co-culture or stimulation method in bovine immature oocytes culture increases the cyclin B1 expression significantly in both experiments (P<0.05). Based on these results, culture condition affects the maturation rate and the expression of cyclin B1 protein during the first meiotic maturation in bovine immature oocytes.

• 한국신뢰성학회지:신뢰성응용연구
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• 제15권1호
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• pp.52-59
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• 2015

In this Study, we tested electroluminescent film to accelerate life by temperature, humidity, voltage, and frequency. We analyzed brightness data to estimate activation energy and verify it's suitability. All of the tests performed in operating condition. Because electroluminescent film is mostly degraded by fluorescent in operating condition. Two different sample groups were tested and compared to find common parameter.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2008년도 International Meeting on Information Display
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• pp.264-267
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• 2008

Through-doping conditions with higher energies and doses were intentionally chosen to understand reverse annealing behavior. We observed that the implantation condition plays a critical role on dopant activation. We found a certain implantation condition with which the sheet resistance is not changed at all upon activation annealing.

• PNF and Movement
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• 제20권2호
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• pp.235-241
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• 2022

Purpose: The purpose of this study was to identify the effects of low intensity blood flow restriction training (LBFR) on the central nervous system of healthy adults. Methods: Ten healthy right-handed adults (eight males and two females, mean age of 28.6 ± 2.87 years) were selected as study subjects. Functional magnetic resonance imaging (fMRI) was conducted to measure brain activation (BA) following LBFR and non-LBFR. The primary motor area, premotor area, and supplementary motor area, which are closely related to exercise, were set as the regions of interest. Results: The BA recorded during the LBFR condition was 931.7 ± 302.44 voxel, and the BA recorded during the non-LBFR condition was 1,510.9 ± 353.47 voxel. Conclusion: BA was lower during LBFR than during non-LBFR.

• 한국운동역학회지
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• 제30권4호
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• pp.311-319
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• 2020

Objective: The purpose of this study was to examine of this study is to study the effect of squat exercise on muscle activation in a sling device using various types of ropes and to propose an effective sling exercise method for strengthening the lower extremity strength. Method: 20 adult male subjects (age: 25.2±2.4 yrs, height: 176.5±3.2 cm, weight: 77.2±4.5 kg) participated in this study. In the experiment, a total of four squats were conducted: squat [SE], sling squat using inelastic rope [IR], sling squat using elastic rope [ER], and sling squat using two elastic ropes [TER]. Squats were performed 5 times for each condition, and a 60-second break was given for each condition to minimize muscle fatigue. The activation of biceps brachii, rectus femoris, gastrocnemius, and tibialis anterior muscles was measured. Results: It was found that the activation of all muscles was the lowest during the squat exercise [SE]. During the sling squat using inelastic rope [IR], the muscle activation of the biceps brachii was the highest. During the sling squat using elastic rope [ER], the activation of the rectus femoris, gastrocnemius, and tibialis anterior muscles was found to be the highest. In the sling squat using two elastic ropes [TER], most of the muscle activation was similar to that of the sling squat using inelastic rope [IR]. Conclusion: Our results of the experiment, it was found that sling squat exercise using elastic rope had a positive effect on the activation of all muscles. It is thought that performing a squat exercise under moderate weight load and unstable conditions, such as sling squat exercise using elastic rope, can increase the muscle activity of the lower limbs and perform more effective exercise effect than performing a conventional squat exercise under stable conditions. In the future, if research is conducted not only on adult men, but also on various ages and patients, it will be able to provide positive help in improving balance, stability and stamina through squat exercise.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 1999년도 춘계학술대회 논문집
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• pp.473-476
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• 1999

PMOS capacitors with Ce-policide electrode were fabricated by the SADS method to study the effects of activation condition on the C-V characteristics. For the activation temperature of $600^{\circ}C$ , the capacitor using CoSi$_2$ formed from Co/Ti bilayer as diffusion source showed excellent C-V properties and the increase in V$_{th}$ with the increasing activation time. But impurties into the oxide.e.

• 말소리와 음성과학
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• 제2권4호
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• pp.3-10
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• 2010

The present study aims to investigate neural activations according to the level of code switching in English proficient bilinguals and to find the relationship between the performance of language switching and proficiency level using ERPs (event-related potentials). First, when comparing high-proficient (HP) with low-proficient (LP) bilingual performance in a native language environment, the activation level of N2 was observed to be higher in the HP group than in the LP group, but only under two conditions: 1) the language switching (between-language) condition known as indexing attention of code switching and 2) the inhibition of current language for L1. Another effect of N400 can be shown in both groups only in the language non-switching (within-language) condition. This effect suggests that both groups completed the semantic acceptability task well in their native language environment without the burden of language switching, irrespective of high or low performance. The latencies of N400 are only about 100ms earlier in the HP group than in the LP group. This difference can be interpreted as facilitation of the given task. These results suggest that HP showed the differential activation in inhibitory system for L1 in switching condition of L1-to-L2 to be contrary to inactivation of inhibitory system for the LP group. Despite the absence of an N400 effect at the given task in both groups, differential latencies between the peaks were attributed to the differences of efficiency in semantic processing.

• The Journal of Korean Physical Therapy
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• 제25권5호
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• pp.316-321
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• 2013

Purpose: The aimed of this study was to investigate muscle activation related to postural stability according to different frequency of whole body vibration during quiet standing, to identify the most effective training conditions that cause the highest neuromuscular responses, and to evaluate the difference of EMG activation according to the anatomical position of the muscle - proximal or distal from the vibration platform. Methods: Eighteen healthy subjects voluntarily participated in this single-group, repeated-measures study in which EMG data from upper trapezius, rectus abdominalis, external oblique abdominalis, elector spinae, gluteus maximus, rectus femoris, semitendinosus, and gastrocnemius were collected over different frequencies (0-5-10-15-20-25Hz) for each subject during quiet standing. Results: We observed a statistically significant difference in the mean values of %RVC of muscular activation according to different frequencies of whole body vibration during quiet standing in all muscles (p<0.05). Conclusion: Our results indicate that lower frequencies of vibration result in low muscular activation, and higher frequencies elicit high muscular activation. However, the most effective training condition that caused the highest activation was 20 Hz. In addition, the proximally located lower extremity muscles (GCM, RF, ST, GM) showed higher activation than the distally located trunk and neck muscles (ES, EO, RA, UT) together with increasing frequency.