• KSBB Journal
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• v.26 no.5
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• pp.407-411
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• 2011

Microbial fuel cell (MFC) wes enriched using sludge in wastewater treatment. The microbial community of activated sludge and enriched MFC were analyzed by FISH (fluorescent in situ hybridization) and 16S rDNA sequencing. Bacteroidetes group were pre-dominant in activated sludge by FISH. ${\alpha}$ group, ${\gamma}$ group and Acintobacter group were dominant and they were similar to distribution. The average value of 10 peak of MFC is 0.44C. When MFC wase enriched by sludge, ${\gamma}$-Proteobacteria, Plantomycetes group increased 70% and 60%, respectively. In results of 16S rDNA sequencing, Sphiringomonas sp. was comprised in ${\alpha}$ proteobacteria and Enterobacter sp., Klebsiella sp., Acinetobacter sp., Bacillus sp. were comprised in ${\gamma}$ proteobacteria and Chryseobacterium sp. was comprised in Flavobacteria were isolated from sludge.

• Journal of Environmental Science International
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• v.14 no.9
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• pp.811-815
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• 2005

The activated sludge from the aeration basin of the Su-yeong municipal wastewater treatment plant which has operated by a standard activated sludge process in Busan, Korea was investigated during April 2004 and January 2005 with several bio-indicators. The number of bacteria and fungi per gram of dry weight of MLSS were estimated to be $3.1\times10^6\sim1.5\times10^8\;and\;l.1\times10^3\sim1.1\times10^5$ colony forming units, respectively, by the plate agar method. By cultivation-independent methods, such as 4',6-diamidino-2-phenylindole stain and fluorescence in situ hybridization, the ratio of eubacteria to the entire biomass was evaluated by more than $80\%$ (v/v). The ratio of ammonia-oxidizing bacteria and nitrite-oxidizing bacteria to the total eubacteria was detennined to be $7.0\sim9.8\%\;and\;3.3\sim6.2\%$ without heavy variation in spite of a period of relatively low temperature in the basin. It would be expected that the nitrification would occur or at least co-exist throughout the year in the sludge of many municipal WWTP with influents that contain the sufficient nitrogen sources although the WWTP does not have any specialized processes for the removal of nitrogen.

• Journal of Environmental Science International
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• v.6 no.3
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• pp.259-265
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• 1997

This study was conducted to Investigate the treatment of wastewater from acetaldehyde manufacturing plant by activated sludge process with Micrococcus roseus AW-6, Micrococcus roseus AW-22, Microbacterium lacticum AW-38 and Mlcrobacterium nae- vaniformans AW-41. The $COD_{Mn}$ and $BOD_5$ of the wastewater were 5, 260mg/L and 6, 452mg/L, respectively. pH was 1.85. The main organic component in the wastewater was acetic acrid which was contained 67, 600mg/L. Optimum dilution time for activated sludge process was shown 10 times. The specific substrate removal rate(BL) was 1.95day-1 and the nonbiodegradable matters(Sn) were 23.2mg/L. Saturation constant (Ks) and mainmum specific growth rate(qmax) were 1, 640mg/L and 2.33day-1, respectively. Sludge yield coefficient(Y) and endogenous respiration coefficient(kd) were 0.28mg MLVSS/mgCOD and 0.02day-1, respectively. $COD_{cr}$ removal efficiency was 91% for 1.95day of hydraulic retention time.

• Korean Journal of Agricultural Science
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• v.19 no.2
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• pp.153-160
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• 1992

To investigate the potential of paper mill sludge and briquet ash as cultural media in turfgrass, zoysiagrass was grown in the mixtures prepared with paper mill sludge and briquet ash. The mixtures were consisted of sludge and briquet ash in the ratio by volume 3:1, 2:1, 1:1, 1:2, and 1:3 in the order. To compare the growth responses, some plants were grown in the medium containing sand(3), field soil(1) and peatmoss (1) by volume anti regarded as control. 1. Activated sludge mixed with sand increased plant height, fresh weight, and dry weight more than 1.5-2.0 times compared to those of the control. The highest plant growth was shown in mixtures containing 67% activated sludge. 2. Plant density per $100cm^2$ and chlorophyll content were higher in all mixtures containing activated sludge than control. 3. The growth of zoysiagrass was reduced along with the increase of non-activated sludge ratio, but no difference was found in chlorophyll content. 4. Plant height, fresh weight and dry weight were greater in activated sludge combined with sand compared to the briquet ash mixtures, but the difference of shoot density between two mixtures was not shown. 5. Even though non-activated sludge appeared not to be appropriate to the growth of seedlings, the number of tillers of plant propagated with rhizome was more increased compared to control when briquet ash content was less than 75%.

• KSBB Journal
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• v.16 no.2
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• pp.133-139
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• 2001

To treat piggery wastewaters containing refractory compounds including nitrogen, biological treatments were investigated. In biological treatment, the removal efficiencies of organics and nitrogen by the activated sludge process and bioreactor using a BACC (Biological Activated Carbon Cartridge) media filled with granular activated carbon were examined. The results were as follows; in the biological process, when the approximate influent BOD concentration of 620 mg/L, through dilution, was treated by the activated sludge process, the process should be operated at a HRT of over 8 days to maintain an effluent BOD concentration of lower than 100 mg/L. In the treatment of piggery wastewater using a BACC bioreactor, when the HRT was 200 hours, the BOD, COD(sub)cr, and TKN removal efficiency of the effluent were 94, 75 and 64.3%, respectively. Comparing the BACC bioreactor with the activated sludge process, when the volumetric loading rate was 0.3 g BOD/L.day, the specific substrate removal rate of BOD was 0.14 g BOD removed/L.day in the activated sludge process which compared with 0.27 g BOD removed/L$.$day in the BACC bioreactor. The BACC bioreactor showed on average a 2-fold higher removal rate and was superior to the activated sludge process in wastewater treatment in terms of variations of loading time and high loading time. Therefore, the BACC process can effectively treat piggery wastewater containing high concentrations of nitrogen and organic compounds.

• Journal of Korean Society of Water and Wastewater
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• v.20 no.5
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• pp.677-684
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• 2006

Electro-flotation(EF), a novel sludge thickening method, could improve the dewaterability of activated sludge. The gas(microbubbles) generated during EF decreased the solid-liquid separation time below 1/5 of the time required for gravity sedimentation. In addition, over 90% of the sludge volume reduction could be achieved by EF although the settling characteristics of the sludge was very poor. The SRF(specific resistance to filtration) of the thickened sludge by EF was much lower than that of the sludge thickened by gravity sedimentation. The SRF of the thickened sludge decreased exponentially with increase of gas generation rate of the EF system. Gas generation rate could be controlled by varying the current density of the electrode. Degasing of the microbubbles by vigorous mixing of the thickened sludge layer deteriorated the dewaterability of the sludge. Therefore, it is obvious that the gas bubbles entrapped in the thickened sludge play a key role in the observed dewaterability improvement.

• Journal of Korean Society on Water Environment
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• v.26 no.2
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• pp.349-355
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• 2010

Electroflotation (EF) was conducted for activated sludge thickening to investigate the effects of sludge SVI (sludge volume index) and chemical conditioning. Return sludge samples were used for the experiment, which were collected from municipal wastewater treatment plants. The performance of sludge thickening was significantly dependent on sludge SVI. For the sludges with SVI values in a range from 50 to about 150 mL/g, the maximum float content decreased rapidly from 8.4 to 3.5% and flotation compressibility followed the same pattern. In cases of sludges with SVI higher than 150 mL/g, those results showed low content levels without large changes. Gas/solids ratio tended to increase with an increase in SVI. When polyelectrolyte was added into sludges for the conditioning, compressibility increased up to 75% and gas/solids ratio was reduced up to about 35% under the condition of microbubble production rate of 530 mL/h, however, there was no consistent effect of chemical conditioning on the maximum float solids content; some cases were positive but the others negative. It was expected that the optimum dose of electrolyte depends on sludge SVI and an excessive chemical dose causes a performance deterioration of flotation thickening.

• Journal of Korean Society on Water Environment
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• v.21 no.2
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• pp.147-152
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• 2005

Fermentation efficiencies of organic wastes from the variety of sources were evaluated based on the production of total volatile acids(TVA) in batch reactor. Mixing and pH were not significant factors in producing TVA from the organic wastes. After a 10-day fermentation, final TVA concentrations in piggery, cattle, poultry, and primary settled sludge of domestic wastewater were 8,900, 2,900, 7,370 and 1,630 mg/L, respectively. The pH of organic wastes was decreased from neutral to 5.7. The ratio of TVA to $NH_4{^+}-N$ produced from the animal waste ranged from 11.5 to 30.1, whereas, that in the primary settled sludge of domestic wastewater, was 5.4. Possibility of fermented organic wastes as the electron donors for denitrification in the activated sludge was investigated. In both acclimated and nonacclimated activated sludge, higher denitrification rates were obtained with fermented piggery sludge added than with either methanol or acetate added. The fermented organic acids derived from the primary settled sludge gave the higher denitrification rate ($4.2mg\;NO_3-N/g\;vss{\cdot}hr$) in the acclimated activated sludge. Denitrification rate was $1.5mg\;NO_3-N/g\;vss{\cdot}hr$ in the nonacclimated sludge with the fermented acids from the primary settled sludge of domestic wastewater added.

• Journal of Korean Society of Water and Wastewater
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• v.23 no.5
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• pp.669-678
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• 2009

The reduction of excess activated sludge from petrochemical plant was investigated by the electro fenton (E-Fenton) process using electrogenerated hydroxyl radicals which lead to mineralization of activated sludge to $CO_2$, water and inorganic ions. Factors affecting the disintegration efficiency of excess activated sludge in E-Fenton process were examined in terms of five criteria: pH, $H_2O_2/Fe^{2+}$ molar ratio, current density, initial MLSS (mixed liquid suspended solids) concentration, $H_2O_2$ feeding mode. TSS total suspended solid and $TCOD_{cr}$ reduction rate increased with the increasing $H_2O_2/Fe^{2+}$ molar ratio and current density until 42 and $6.7 mA/cm^2$, respectively but further increase of $H_2O_2/Fe^{2+}$ molar ratio and current density would reduce the reduction rate. On the other hand, as expected, increasing pH and initial MLSS concentration of activated sludge decreas TSS and $TCOD_{cr}$ reduction rate. The E-Fenton process was gradually increased during first 30 minutes and then linearly proceed till 120 minutes. The optimal E-Fenton condition showed TSS reduction rate of 62~63% and $TCOD_{cr}$ (total chemical oxygen demand) reduction rate of 55~56%. Molar ratio $H_2O_2/Fe^{2+} = 42$ was determined as optimal E-Fenton condition with initial $Fe^{2+}$ dose of 5.4 mM and current density of $6.7{\sim}13.3 mA/cm^2$, initial MLSS of 7,600 mg/L and pH 2 were chosen as the most efficient E-Fenton condition.

• Journal of Animal Environmental Science
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• v.12 no.1
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• pp.29-34
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• 2006

The effects of turning frequency were examined on the efficiency of composting lime treated excess sludge amended with sawdust from the activated sludge process after a liquid/solids separation process. The raw and excess sludge from the activated sludge process associated with the hog wastewater treatment system is a significant problem and composting is an effective method far reducing the pollution potential of hog wastewater sludge. The coagulant used sludge composting and ammonia emissions from composting are not well established. The effect of compost properties such as high total carbon, C/N ratio and pH value on performance of composting sludge and biofiltration of ammonia from composting process were investigated. The ammonia emission was not significantly increased during composting. The ammonia concentrations of the exhaust air of composter were ranged from 0.5 and 7 ppm about 12 days after composting. The performance of the hog wastewater sludge composting was the most sensitive to chemical treated sludge properties such as high total carbon and high C/N ratio of the initial compost mixes. Temperature in compost and ammonia emission were not greatly affected by the turning frequency.