• Proceedings of the Korean Society of Crop Science Conference
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• 2004.04a
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• pp.12-27
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• 2004

Thanks to spectacular advances in the techniques for identifying proteins separated by two-dimensional electrophoresis and in methods for large-scale analysis of proteome variations, proteomics is becoming an essential methodology in various fields of plant sciences. Plant proteomics would be most useful when combined with other functional genomics tools and approaches. A combination of microarray and proteomics analysis will indicate whether gene regulation is controlled at the level of transcription or translation and protein accumulation. In this review, we described the catalogues of the rice proteome which were constructed in our program, and functional characterization of some of these proteins was discussed. Mass-spectrometry is a most prevalent technique to identify rapidly a large of proteins in proteome analysis. However, the conventional Western blotting/sequencing technique us still used in many laboratories. As a first step to efficiently construct protein data-file in proteome analysis of major cereals, we have analyzed the N-terminal sequences of 100 rice embryo proteins and 70 wheat spike proteins separated by two-dimensional electrophoresis. Edman degradation revealed the N-terminal peptide sequences of only 31 rice proteins and 47 wheat proteins, suggesting that the rest of separated protein spots are N-terminally blocked. To efficiently determine the internal sequence of blocked proteins, we have developed a modified Cleveland peptide mapping method. Using this above method, the internal sequences of all blocked rice proteins (i. e., 69 proteins) were determined. Among these 100 rice proteins, thirty were proteins for which homologous sequence in the rice genome database could be identified. However, the rest of the proteins lacked homologous proteins. This appears to be consistent with the fact that about 30% of total rice cDNA have been deposited in the database. Also, the major proteins involved in the growth and development of rice can be identified using the proteome approach. Some of these proteins, including a calcium-binding protein that fumed out to be calreticulin, gibberellin-binding protein, which is ribulose-1,5-bisphosphate carboxylase/oxygenase activate in rice, and leginsulin-binding protein in soybean have functions in the signal transduction pathway. Proteomics is well suited not only to determine interaction between pairs of proteins, but also to identify multisubunit complexes. Currently, a protein-protein interaction database for plant proteins (http://genome .c .kanazawa-u.ac.jp/Y2H)could be a very useful tool for the plant research community. Recently, we are separated proteins from grain filling and seed maturation in rice to perform ESI-Q-TOF/MS and MALDI-TOF/MS. This experiment shows a possibility to easily and rapidly identify a number of 2-DE separated proteins of rice by ESI-Q-TOF/MS and MALDI-TOF/MS. Therefore, the Information thus obtained from the plant proteome would be helpful in predicting the function of the unknown proteins and would be useful in the plant molecular breeding. Also, information from our study could provide a venue to plant breeder and molecular biologist to design their research strategies precisely.

• Korean Security Journal
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• no.16
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• pp.161-187
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• 2008

The purpose of this study is to suggest a development method of current Korean security system by analyzing the problems shown in the performance of security work in relation to the terrorism, which is enlarging in the word, from various aspects. In order to perform the study, the researcher considered the basic theory concerned to current Korean law concerned to security, principle and methodology of security, terror and new terrorism. The researcher performed the study by selecting qualitative case study focused on Park Geun-Hye case. Through the study, the methods to develop Korean security system are as follows. First, from the legal aspect, it is necessary to establish the law concerned to terrorism prevention and important person security. Moreover, it is necessary to search for the development of private security by revising Security Industry Act, which is a legal ground of private security. Second, it is necessary to improve and reinforce education & training program, which is not still divided in detail from the aspect of private security cultivation. Moreover, it is necessary to activate personal protection work and enlarge market through Security Industry Act and make an effort to change social recognition over security, which is devaluated in the society. From the viewpoint, national license about private security shall be adopted. The department of president security, which is a representative of official security, shall transfer the advanced technology to private security organization. Third, from the aspect of operation, the operation of security based on SCE principle, human shield principle, the nearest person's protection principle, body extension principle, linear protection principle and evacuation priority principle is required. Therefore, the priority shall be given to preventive security and thorough security plan shall be made for the operation.

• Journal of Digital Convergence
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• v.17 no.11
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• pp.483-492
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• 2019

The culture and education are very active with the active policy and support to form the government's Maker Movement. The purpose of this study is to grasp the current status of the education sector of the domestic maker movement, which is increasing recently, and to propose a plan for activating maker education for the development of a positive direction. To this end, first, the current status and problems of domestic maker training are derived through in-depth interviews with existing maker training operators and participants. Second, based on the contents of the interview script, keyword analysis and its characteristics through the qualitative survey analysis program (NVIVO) are identified. Third, based on the analysis results, we propose a plan and development direction for domestic maker education. Based on the educators who performed maker training and the students involved, professional maker teachers were required for the professionalism of education, and the expansion of maker channels and professional networking of participating students was required. In addition, there was a need for specialized programs and appropriate policy support that reflected the characteristics of maker training. This study aims at contributing to the activation of maker education, which is a major field of maker movement, by helping to improve concrete support methods, training related educators, and educational environment for maker education.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.3
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• pp.407-416
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• 2019

This study is about a Customized Nursery School where working women can support work - life balance and a reduction in retirement or turnover. The research method is to identify the current status of Customized Nursery services and to recognize the recognition and need of the operation of Customized Nursery School. The importance of securing skilled nurses and preventing them from changing their jobs for the health and safety of people cannot be emphasized enough. A Customized Nursery School must be opened to reduce the retirement or change of jobs of working women nurses and to provide care for continuous work in three shifts from 365days to support the balance between the working mother and family. It is considered that nurses will put their children in relief when using retired nurses who have the ability to work 24hour rotation in a Customized Nursery School and when a Customized Nursery School be ran suited for the condition and demand of working women nurses, it is expected to reduce retirement and the change of jobs, also to give positive effect on marrige and family planning which would make improvement in low birthrate. To activate the Customized Nursery School, Creating a secure learning environment and qualification of educators great effort should be put. A program curriculum based on 'basic life and habits' should be the center of education. Continuous management and effort will need to be placed in continuous development of educators.

• 재활복지
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• v.21 no.4
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• pp.177-193
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• 2017

The purpose of this study was to examine the factors that affect the awareness of disabled in undergraduate students. A sample of 1,998 respondents was surveyed. Of these, a total of 1,957 questionnaires were analysed excluding the 41 questionnaires that did not agree with the survey or had a missing data. The questionnaire consists of the general characteristics, experience related to disability and disability awareness and we used a 4-point Likert scale. Collected data was analyzed using the SPSS 24.0 program and descriptive statistics, t-test, variance analysis and multiple regression analysis were used. According to the results of the study, the disability awareness score of our respondents was average 40.52. Second, female students had more positive about disability awareness than male ones, and senior students had more positive awareness than other grade students. Third, non-rehabilitation department students' disability awareness was more positive than rehabilitation students. Fourth, the disability awareness of people who have a acquaintance member with disability or health worker were significantly positive. Finally, the awareness of people who experienced at the disability related institution were significantly positive. Therefore, to improve the awareness of undergraduate students about disabilities, it is required to activate positive contact experiences with people with disabilities rather than theoretical learning.

• Journal of the Korean Society of Radiology
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• v.13 no.4
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• pp.605-611
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• 2019

The purpose of this study is to manufacture a multi-function device (MFD) which can be applied to various types of weight-bearing view of the lower leg, and to compare the results with the images from the existing weight-bearing platforms (WBPs), thereby suggesting a clinical utilization. The MFD was manufactured, by considering the minimum adjustable heights of the platform for weight-bearing foot/ankle, platform for hindfoot alignment view, and X-ray tube of the X-ray device. A foot/ankle phantom was used to take the images of weight-bearing lateral foot in MFD and WBPs to compare the resolutions of the X-ray images using a quick modulation transfer function (MTF) program. Between both the images taken from the MFD and WBPs, there was no statistically significant difference found in the mean cycles per pixel (C/P) and the lines per image height (LPH) of the 50%-Contrast Spatial Frequency (MTF50), and 10-90% of Maximum Energy Rise Distance (10-90%), where p>0.05. The MFD is suggested for its clinical trial as a useful positioning device that can secure the patient's safety and manifoldly perform various inspections. Also, the recommendation of the positioning device as a policy can activate dedicated manufacturers, while also improving the quality of medical services.

• Journal of Korea Entertainment Industry Association
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• v.15 no.2
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• pp.213-222
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• 2021

The purpose of this study is to provide basic data for practical measures to improve life satisfaction through appropriate information utilization ability for the elderly by specifically grasping the effect of information utilization ability of the elderly on life satisfaction. The subjects of this study were 220 elderly people aged 65 and over who live in J city, Chungbuk. In order to examine the effects of the information literacy of the elderly on life satisfaction, frequency analysis, descriptive statistics analysis, correlation analysis, and multiple regression analysis were conducted. For statistical analysis, SPSS 20.0 program was used for analysis. The results of this study showed that information technology and information literacy were significant factors affecting life satisfaction among the information literacy abilities of the elderly. This means that the higher the information technology ability and information utilization ability in the information utilization ability of the elderly, the higher the life satisfaction. Especially, information literacy has a greater effect on life satisfaction than information technology ability. However, information provision ability and information criticism ability did not have a significant effect on life satisfaction. Based on the results of this study, policy suggestions are as follows: First, it is necessary to activate information education to improve the information literacy of the elderly. Second, it is necessary to build information infrastructure to improve the information technology ability of the elderly.

• 지역과문화
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• v.7 no.1
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• pp.153-182
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• 2020

Andong is the place where the most ginger is produced in Korea. The article is based on a study on the development of storytelling of a co-brand of local agricultural products, focusing on the case of 'The Geudae Ginger,' a co-brand of ginger in Andong. This study aims to develop a brand storytelling of Andong Ginger's co-branded 'Geudae Ginger' to build an image as a local specialty and help revitalize the Andong ginger's industry. The process of developing storytelling to activate 'Geudae Ginger' brand is as follows. In the first step, I collected storytelling materials through data research. Ginger, which has long been used as a medicine for mankind, has more historical and cultural stories than anything else. In the second step, story resources were extracted based on data research. By analyzing the story properties of Andong ginger, we made its list. As a result, the image of the nobility, rigidity and chastity of ginger, which is used to benefit all over, could be associated with the image of Andong, the capital of Korean spiritual culture. Storytelling was developed in the third step. The main theme was 'Andong ginger with anther level ' and the main story was 'The Story of Andong's Ginger Teacher'. The scenario developed is as follows: 1. Introducing Andong's Ginger Teacher, 2. The birth of Dosan Thirteen Tea, 3. 'Geudae Ginger' that bridges love. In the last fourth step, I proposed ways to utilize storytelling. I presented the spread methods of consumer-participated storytelling using images of 'Geudae Ginger' and a new-tro event with teachers highlighting the image of 'Ginger Teacher' and others as a local business program for storytelling expansion.

• Journal of Practical Engineering Education
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• v.14 no.1
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• pp.37-48
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• 2022

This study analyzed the effect of internal audit on the performance of the IATF 16949 automotive quality management system to understand the internal audit of companies and propose measures to activate the company's internal audit process. It was identified with the empirical analysis that 'The internal auditor competence, internal audit planning, internal audit implementation, infrastructure, culture/environment, and CEO support' to characterize IATF 16949 internal audit of automotive quality management system affects the internal performance and business performance of the company. In addition, I checked the size of the company and the period of certification period as moderating variables according to the sales based on the presented as factors that can improve the performance of the company, and how the moderating effects are seen in the relationship with the performance of the organization. I did analysis of technical statistics, exploratory factors, reliability, and multi-regression analysis with SPSS program. I summarized the results of the study, as a result of that, it was found that the internal audit planning, internal audit implementation, culture/ environment, and CEO support of independent variables affected the parameter and dependent variables (the internal performance and management performance of companies).

• Journal of the Korean Society for information Management
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• v.39 no.1
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• pp.17-44
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• 2022

The purpose of this study was to present a plan on the effect of non-face-to-face services on library anxiety facilities by analyzing the library anxiety factors of university library users. To this end, we look at the cases of university library user service response activities in response to the COVID-19 crisis and select 40 schools with the highest number of library visitors per student from among domestic four-year university libraries with 5,000 or more and less than 10,000 students. Methods of information service and program cases were analyzed, and K-LAS was reconstructed and surveyed for current students using the K university library, and frequency analysis, descriptive statistical analysis, exploratory factor analysis, and reliability analysis, correlation analysis, and multiple regression analysis were applied to analyze the library anxiety factors of users. Identify the relationship between 5 library anxiety factors and non-face-to-face service activation factors, such as physical/environmental factors of the library, data search selection factors, digital information system factors, librarian (staff) factors, and psychological/emotional factors, and activate non-face-to-face services. The influence of these factors on library anxiety factors was examined, and as a result, it was found that non-face-to-face service activation factors had the greatest influence on library digital information system anxiety factors. Based on the analysis results, it was attempted to derive a plan to relieve users' library anxiety by activating non-face-to-face services.