• BMB Reports
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• v.37 no.4
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• pp.422-428
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• 2004

Raw-starch-digesting $\alpha$-amylase (Amyl III) was purified to an electrophoretically pure state from the extract of a koji culture of Aspergillus awamori KT-11 using wheat bran in the medium. The purified Amyl III digested not only soluble starch but also raw corn starch. The major products from the raw starch using Amyl III were maltotriose and maltose, although a small amount of glucose was produced. Amyl III acted on all raw starch granules that it has been tested on. However, it was considered that the action mode of the Amyl III on starch granules was different from that of glucoamylase judging from the observation of granules under a scanning electron microscope before and after enzyme reaction, and also from the reaction products. Glucoamylase (GA I) was also isolated and it was purified to an electrophoretically pure state from the extract. It was found that the electron micrographic features of the granules after treatment with the enzymes were quite different. A synergistic effect of Amyl III and GA I was observed for the digestion of raw starch granules.

• The Korean Journal of Physiology and Pharmacology
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• v.22 no.3
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• pp.269-275
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• 2018

$Na^+/H^+$ exchangers (NHEs) have been shown to be involved in regulating cell volume and maintaining fluid and electrolyte homeostasis. Pooled evidences have suggested that loss of $Na^+/H^+$ exchanger isoform 8 (NHE8) impairs intestinal mucosa. Whether NHE8 participates in the pathology of infectious colitis is still unknown. Our previous study demonstrated that somatostatin (SST) could stimulate the expression of intestinal NHE8 so as to facilitate $Na^+$ absorption under normal condition. This study further explored whether NHE8 participates in the pathological processes of infectious colitis and the effects of SST on intestinal NHE8 expression in the setting of infectious colitis. Our data showed that NHE8 expression was reduced in Citrobacter rodentium (CR) infected mice. Up-regulation of NHE8 improved diarrhea symptom and mucosal damage induced by CR. In vitro, a similar observation was also seen in Enteropathogenic E. coli (EPEC) infected Caco-2 cells. Seglitide, a SST receptor (SSTR) 2 agonist, partly reversed the inhibiting action of EPEC on NHE8 expression, but SSTR5 agonist (L-817,818) had no effect on the expression of NHE8. Moreover, SST blocked the phosphorylation of p38 in EPEC-infected Caco-2 cells. Taken together, these results suggest that enhancement of intestinal NHE8 expression by SST could ameliorate the symptoms of mice with infectious colitis.

• Journal of the Korea Convergence Society
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• v.12 no.9
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• pp.57-63
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• 2021

This study dissected and three-dimensionally (3D) scanned the facial muscles of Korean adult cadavers, created a three-dimensional model with realistic facial muscle shapes, and reproduced facial expressions to provide educational materials to allow the 3D observation of the complex movements of cadaver facial muscles. Using the cadavers' anatomical photo data, 3D modeling of facial muscles was performed. We produced models describing four different expressions, namely sad, happy, surprised, and angry. We confirmed the complex action of the 3D cadaver facial muscles when making various facial expressions. Although the results of this study cannot confirm the individual functions of facial muscles quantitatively, we were able to observe the realistic shape of the cadavers' facial muscles, and produce models that would show different expressions depending on the actions performed. The data from this study may be used as educational materials when studying the anatomy of facial muscles.

• Journal of the Korea Convergence Society
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• v.10 no.8
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• pp.121-128
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• 2019

Medical services should be provided not only to patients but also to medical staff who are the main actors of medical treatment. This paper is based on Ethnography, observing the behavior of medical staff performing medical actions and finding what is the most frequent of physical medical treatment. In particular, I observed the need for recognition and performance of the clinical workforce for invasive medical treatment. Based on the Insight from the observation and analysis of medical staff's behavior on intravenous fluid therapy and in-depth interview, I defined four design value factors. Plus, I suggest a design prototype with a proposal for designing a medical consumable kit for intravenous fluid therapy to validate the design elements. Kitting medical consumables simplify the preparation and disposal process throughout the treatment, enabling rapid medical action and increasing work efficiency.

• Animal Bioscience
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• v.34 no.1
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• pp.143-153
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• 2021

Objective: To explore the molecular mechanisms of fatty liver hemorrhagic syndrome (FLHS) in laying hens, an experiment was conducted to reveal the differences in histopathological observation and gene expression between FLHS group and normal group. Methods: We compared the histopathological difference using hematoxylin and eosin staining and proceeded with RNA sequencing of adipose tissue to search differentially expressed genes and enriched biological processes and pathways. Then we validated the mRNA expression levels by real-time polymerase chain reaction and quantified protein levels in the circulation by enzyme-linked immunosorbent assay. Results: We identified 100 differentially expressed transcripts corresponding to 66 genes (DEGs) were identified between FLHS-affected group and normal group. Seven DEGs were significantly enriched in the immune response process and lipid metabolic process, including phospholipase A2 group V, WAP kunitz and netrin domain containing 2, delta 4-desaturase sphingolipid 2, perilipin 3, interleukin-6 (IL-6), ciliary neurotrophic factor (CNTF), and suppressor of cytokine signaling 3 (SOCS3). And these genes could be the targets of immune response and be involved in metabolic homeostasis during the process of FLHS in laying hens. Based on functional categories of the DEGs, we further proposed a model to explain the etiology and pathogenesis of FLHS. IL-6 and SOCS3 mediate inflammatory responses and the satiety hormone of leptin, induce dysfunction of Jak-STAT signaling pathway, leading to insulin resistance and lipid metabolic disorders. Conversely, CNTF may reduce tissue destruction during inflammatory attacks and confer protection from inflammation-induced insulin resistance in FLHS chickens. Conclusion: These findings highlight the therapeutic implications of targeting the JAK-STAT pathway. Inhibition of IL6 and SOCS3 and facilitation of CNTF could serve as a favorable strategy to enhance insulin action and improve glucose homoeostasis, which are of importance for treating obesity-related disorders for chickens.

• Journal of Microbiology and Biotechnology
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• v.31 no.5
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• pp.659-666
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• 2021

After Candida albicans, Candida glabrata is one of the most common fungal species associated with candidemia in nosocomial infections. Rapid acquisition of nutrients from the host is important for the survival of pathogens which possess the metabolic flexibility to assimilate different carbon and nitrogen compounds. In Saccharomyces cerevisiae, nitrogen assimilation is controlled through a mechanism known as Nitrogen Catabolite Repression (NCR). NCR is coordinated by the action of four GATA factors; two positive regulators, Gat1 and Gln3, and two negative regulators, Gzf3 and Dal80. A mechanism in C. glabrata similar to NCR in S. cerevisiae has not been broadly studied. We previously showed that in C. glabrata, Gln3, and not Gat1, has a major role in nitrogen assimilation as opposed to what has been observed in S. cerevisiae in which both factors regulate NCR-sensitive genes. Here, we expand the knowledge about the role of Gln3 from C. glabrata through the transcriptional analysis of BG14 and gln3Δ strains. Approximately, 53.5% of the detected genes were differentially expressed (DEG). From these DEG, amino acid metabolism and ABC transporters were two of the most enriched KEGG categories in our analysis (Up-DEG and Down-DEG, respectively). Furthermore, a positive role of Gln3 in AAA assimilation was described, as was its role in the transcriptional regulation of ARO8. Finally, an unexpected negative role of Gln3 in the gene regulation of ABC transporters CDR1 and CDR2 and its associated transcriptional regulator PDR1 was found. This observation was confirmed by a decreased susceptibility of the gln3Δ strain to fluconazole.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.32 no.1
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• pp.267-293
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• 2021

This study is aimed at analyzing the achievement criteria of cross-curricular Learning themes that can be linked to curriculums, and developing and applying the career reading programs focusing on the school library. For these purposes, first, a class model was designed in connection with reading education programs through cross-curricular Learning themes of 2015 revised curriculum. Second, the career reading classes in the areas of self-understanding, career exploration and career design have been held through a career reading class model for 137 middle school students in 5 classes for one semester. Third, the data to evaluate the career reading for the analysis of the study was constructed, and the qualitative study was conducted through of anecdotal records, multilateral observation on checklists and the analysis of action research. Lastly but not least, the materials regarding the list of books focusing on the career reading storytelling and the career mentor in a type of interests were presented, and the career reading education activities at the school field were suggested.

• Journal of the Korean Society of Physical Medicine
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• v.18 no.3
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• pp.47-53
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• 2023

PURPOSE: A visual feedback method was proposed to induce brain stimulation in a stroke patient, and among them, there was a treatment using a mirror. On the other hand, mirror therapy focuses only on the functional changes in body movements, and analysis of neurophysiological mechanisms of brain activity is lacking. In addition, studies on evaluating the activity and response generated in specific brain regions during visual feedback training using mirrors are insufficient. METHODS: Fifteen healthy adults (male: 10, female: 5, Years: 23.33 ± 1.23), who were right-handed were recruited. By attaching the C3, Cz, and C4 channels in the sensorimotor cortex using an electroencephalogram, training was performed under the conditions without mirror-based visual feedback (No-condition) and with visual feedback (Tasks-condition). At this time, the immediate activity of the mu-rhythm in response to training was separated and evaluated. RESULTS: The tasks-condition of C3, Cz, and C4 channels activated the relative mu-rhythm rather than the no-condition, and all showed significant differences (p < .05). In addition, in all channels at the start time, the tasks-condition was more active than the no-condition (p < .05). The activity of the cortical response was higher in the tasks-condition than in the no-condition (p < .05). CONCLUSION: The mu-rhythm activity can be evaluated objectively when visual feedback using a mirror is applied to healthy subjects, and a basic analysis protocol is proposed.

• The Journal of the Convergence on Culture Technology
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• v.9 no.6
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• pp.137-144
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• 2023

The purpose of this study attempted to investigate the degree and relationship of health knowledge, health beliefs, and preventive health behavior of public health center officials and provide basic data to develop measures to promote preventive health behavior of public health center officials. Factors affecting health behavior were identified as sub-factors of health beliefs: benefit (β = . 298), severity (β = . 193), and trigger for action (β = . 183). The explanatory power of preventive health behaviors between these variables was found to be 33.9%. This study was attempted in the absence of research on COVID-19 knowledge, health beliefs, and preventive health behaviors among public health center officials, and objective observation or qualitative research that could supplement the limitations of self-reported questionnaires was conducted to 19 Research exploring the meaning of preventive health behaviors is needed.

• Hwankyungkyoyuk
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• v.3 no.1
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• pp.55-62
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• 1992

In the orient, ancestor's perception to natural environment was related on the change of climate. Man had been a essence of the warm blood animal, which had suited in mild climate for living and acting. The weather of hot or cold was not only comfort in human life but influences negatively on human behavior. The most people of oriental was particularly interrelated to seasonal change of climate in earlier time. Because, there are elements such as temperature, humidity and wind in the category of climate, these elements differentiated seasonal change. The main methods of perception of natural environment were observed and classified. Although these methods were in the primitive stage, these coincided the tendency of modern science. For example, confucian was recognized the law of vertical air current that warm air rised and cold air sinked. And they could classify all elements nature based on the principles of 'Yin and Yang(陰陽論)' such as male and female, the sun and moon. shade and light etc. Thus results of the observation and the classification concerned with physical environment can be utilize a wisdom for progressive life of inhabitants. It was a origin of the education in natural environment. Commonness in the viewpoint of environment in the orient if recognized the law of circulation. Buddhism, originated in india, realized that all of phenomena in the world was changed slowly through the principles of cycle(輪廻說) such as male and female, the sun and moon, shade and light etc. Thus results of the observation and the classification concerned with physical environment can be utilized a wisdom for progressive life of inhabitants. It was a origin of the education in natural environment. Commonness in the viewpoint of environment in the orient if recognized the law of circulation. Buddhism, originated in India, realized that all of phenomena in the world was changed slowly through the principles of cycle(輪廻說). For example, whole of land is shifted from young stage to old stage as the life cycle of humanbeing. The theory of karma effects(葉報設) is so signify with the good result that good one's action is reap a sweet fruit. The most environmental problem in today must realized as a consequence of men's act. Then Taoism emphasized the pure nature without the artificiality. Because complexual environment was composed of several elements, It was maintained through the artificiality. Because complexual environment was composed of several elements, It was maintained through the interaction of cause and effects. The solution of environmental problem is maintenanced the harmony between cause and effects by a philosophical concept.