• Restorative Dentistry and Endodontics
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• 제21권1호
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• pp.121-135
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• 1996

Actinomyces are Gram-positive, non-acid-fast, anaerobic or microaerophilic filamentous bacteria. These organisms are frequently detected from infected root canals and periapical lesion. The purpose of this study was to use indirect immunofluorescence to determine the prescence of select Actinomyces species in a survey of teeth associated with periapical lesion, to clarify the relationship between clinical symptoms of periapical lesions and the Actinomyces species and to study on the cross reaction among Actinomyces. Actinomyces israelii serotype I (ATCC 12102), Actinomyces israelii serotype II (ATCC 29322), Actinomyces viscosus serotype II (ATCC 19246), Actinomyces naslundii serotype I (ATCC 12104) were cultured in anaerobic condition. Rabbit antisera were prepared by intravenous injection of formalized whole cells. Indirect immunofluorescence method was used to achieve the purpose. The following results were obtained. 1. There was a relationship between Actinomyces and periapical disease. 2. A. israelii serotype I, II were frequently identified with Indirect Immunofluorescence and most often assosiated with periapical disease. In culture finding, there was no significant difference between each group. 3. Indirect Immunofluoresence is both more sensitive and more rapid than culture for identification of Actinomyces species in patients with periapical lesion. 4. A. israelii serotype I, II was highly isolated in infected root canals with local swelling, A. naslundii serotype I was highly isolated in those with foul odor, and A. israelii serotype I was found in higher frequncy in those with exudate than other bacteria. 5. In the Indirect Immunofluorescence (1 : 320), A positive cross reaction was obtained between A. israelii serotype I and A. israelii serotype II, also, A. viscosus serotype II and A. naslundii serotype I. There was no cross reaction between A. israelii serotype I, II and A. viscosus serotype II, A. naslundii serotype I.

• 대한소아치과학회지
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• 제36권1호
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• pp.12-19
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• 2009

본 연구는 black stain을 가진 유치 치태에서 추출한 방선균이 S. mutans에 대해 항생능을 가지는가를 평가하고자 시도되었다. 만 $2{\sim}6$세 유치열기 어린이 중 black stain이 모든 치아에 존재하는 4명의 어린이를 대상으로 치태를 채취한 후, 증균 배양하여 16개의 방선균 균주를 분리해 냈으며, 이들이 생산해내는 물질이 항생능을 가지는지를 paper disc method를 이용하여 확인하여, 다음과 같은 결론을 얻었다. 1. No.1 균주와 No.5 균주에서 S. mutans에 항생능을 보였으며, 특히 No.5 균주는 Oxacillin과 유사한 항생능을 보였다. 2. No.1 균주와 No.5 균주는 일반적으로 항생능 평가시 사용되는 시험균인 Bacillus cereus, Bacillus subtilis에도 항생능을 보였다. 3. PCR을 통해 구강내 대표적인 상주 방선균들을 대상으로 이 균주들의 동정을 시도해본 결과 No.5 균주는 Actinomyces viscosus와 97% 일치하는 것으로 나타났다.

• 한국치위생학회지
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• 제17권1호
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• pp.111-122
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• 2017

Objectives: Coriolus versicolor is an edible mushroom with physiological activities that has been used in traditional medicine. The aim of this study was to investigate the antimicrobial activity of extracts obtained from Coriolus versicolor against oral pathogens. Methods: The antimicrobial activities of various extracts of Coriolus versicolor were examined by disc diffusion assay, and minimum inhibitory concentration (MIC) of these extracts were also was determined by broth dilution method. The growth inhibition effect of extracts was measured at 600 nm for 12 hrs against Streptococcus ratti, Streptococcus criceti, Aggregati--bacter actinomycetemcomitans, Actinomyces viscosus, and Actinomyces israelii. Results: Coriolus versicolor extracts showed antimicrobial activities against all nine oral pathogens through disc diffusion assay. The ethanol extract and ethyl acetate extract differed significantly compared with acetone extract against Streptococcus ratti, Streptococcus criceti, Actinomyces viscosus, Actinomyces israelii and Aggregatibacter actinomycetemcomitans (p<0.05). These extracts exhibited MIC ranges of 2.63 to >10.50 mg/ml against the tested bacteria. The ethanol extract from Coriolus versicolor showed lower MIC values of 2.63 to 5.25 mg/ml. According to the obtained growth curve, the extracts of Coriolus versicolor were more effective against Actinomyces viscosus. Conclusions: The acetone, ethanol, and ethyl acetate extracts from Coriolus versicolor showed antimicrobial activities against Streptococcus mutans, Staphylococcus aureus, Streptococcus sanguinis, Streptococcus sobrinus, Streptococcus ratti, Streptococcus criceti, Aggregatibacter actinomycetemcomitans, Actinomyces viscosus, and Actinomyces israeli.i Therefore, they could be considered as natural oral antimicrobial agents against oral pathogens.

• 한국식품영양학회지
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• 제13권2호
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• pp.171-175
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• 2000

Microbial proteases have certain unique characteristics, and are now widely used in food, leather, detergent, and pharmaceutical industries. Thermophilic Actinomyces producing the protease was isolated from soil in Wonju city. This strain was able to grow and produce protease at the culture temperature of 50$^{\circ}C$. The maximum protease production was obtained when 0.5% soluble starch and 0.4% yeast extract were used as carbon and nitrogen source, respectively. The other culture condition for the maximal productivity of the protease was 0.1% K2HPO4, and 0.05% CaCl2 at initial pH 8.0 for 48 hours.

• Journal of Chest Surgery
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• 제48권1호
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• pp.86-89
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• 2015

Primary sternal osteomyelitis is a rare disease. Common infectious organisms causing primary sternal osteomyelitis include Staphylococcus aureus and Pseudomonas aeruginosa. Actinomyces species are common saprophytes of the oral cavity, but there have been few reports in the literature of primary sternal osteomyelitis caused by Actinomyces species. We describe a case of primary sternal osteomyelitis caused by Actinomyces israelii without pulmonary involvement.

• 미생물학회지
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• 제55권1호
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• pp.74-76
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• 2019

그람 양성 혐기성 간균 Actinomyces는 구강 인두, 위장관 및 비뇨 생식 기관의 점막 표면에서 흔히 서식한다. 본 논문에서는 한국 노인 여성의 치태에서 분리된 Actinomyces georgiae KHUD_A1의 유전체 염기서열을 분석하여 보고한다. 이 균주의 유전체는 2,652,059 bp의 크기로 GC 함량은 68.06%이며, CPBP family intramembrane metalloprotease 유전자와 bile acid: sodium symporter family protein 유전자 등 157개의 KHUD_A1 균주 특이적인 유전자들을 포함한다. 이 유전체의 서열 정보는 A. georgiae종의 일반적인 특성과 Actinomyces속의 유전체 다양성을 이해하는데 유용한 정보를 제공할 것이다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제23권1호
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• pp.82-86
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• 2001

저자 등은 하악 우측 제3대구치 발거 후 발치와를 통해 하악골에 이환되어 발생한 경안부 방선균증에서 병소부의 적출술과 소파술을 시행하고, 항생제 요법 및 개방창 유지등의 방법을 통해 양호한 경과를 보였기에 문헌고찰과 함께 보고하는 바이다.

• Journal of Microbiology and Biotechnology
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• 제4권2호
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• pp.102-107
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• 1994

The $\beta$-1,4-endoglucanase gene from Actinomyces sp. 40 was cloned into Escherichia coli DH5$\alpha$ with pUC19. Chromosomal DNA from Actinomyces sp. 40 was cleaved with the restriction enzyme Sau3AI and ligated into pUC19 for the transformation of Escherichia coli DH5$\alpha$. Positive clones of $\beta$-1,4-endoglucanase gene were detected as the clear zones on a medium supplemented with carboxymethylcellulose (CMC). This transformant possessed a single plasmid, designated pDS1, which contained the vector DNA and a 3.5 kilobase (kb) Sau3AI insertion fragment encoding endoglucanase. The size of the cloned fragment was reduced to 2.0 kb. The endoglucanase activity produced by the E. coli DH5$\alpha$ (pDS6) was higher than that of Actinomyces sp. 40 strain. The optimum pH and temperature of the cloned enzyme were pH 4.0$\sim$5.0 and 55$^{\circ}C$, respectively. The cloned enzyme was stable at 55$^{\circ}C$ or below and in buffer ranging from pH 4.0 to 7.0. The enzyme degraded CMC but did not degrade xylan, cellobiose, and methyl-umbelliferylcellobiopyranoside (MUC).

• 한국식품영양학회지
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• 제13권2호
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• pp.176-180
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• 2000

Microbial protease has been interesting due to the biological roles in the producing microorganism. A thermophilic Actinomyces produing protease was isolated from soil. The optimal medium composition and culture conditions for maximum protease production was as follows 0.5% soluble starch, 0.5% yeast extract. 0.1% K2HPO4, 0.05% CaCl2, initial pH 8.0 at 50$^{\circ}C$ for 48hours. The protease was purified by the procedure of ammonium sulfate precipitation, anion exchange chromatography(LC), DEAE high performance liquid chromatography and GPC HPLC. The purification fold of the purified enzyme was increased about 22.6. The optimal pH and temperature for reaction of the purified enzyme were 7.5 and 60$^{\circ}C$. The purified enzyme was stable for the pH range from 6.0 to 8.5, but was unstable when treated at 80$^{\circ}C$ for 10 minutes. The activity of the enzyme was inhibited by Ag+ and Cu2+.

• 대한치과교정학회지
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• 제27권2호
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• pp.173-180
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• 1997

본 연구는 교정환자를 대상으로 chlorhexidine varnish를 치면에 도포했을 때 치태내 균주의 변화양상을 관찰하여 교정치료환자에 있어 치아우식증예방을 위한 chlorhexidine 사용의 효용성을 평가하고자 하는데 그 목적이 있다. 서울대학교 병원 치과진료부 교정과에 내원한 신환 26명을 대상으로 각 13명씩을 실험군과 대조군으로 구분하여 실험군에는 chlorhexidine varnish를 1주일 간격으로 4희 도포하고 대조군에는 같은 방법으로 placebo varnish를 도포하여 처치 전과 처치 4주후, 처치 8주후의 치태내 균주 변화양상을 간접면역형광현미경법으로 분석하였다. 결과는 다음과 같다. 1. Chlorhexidine varnish 처치에 의해 Streptococcus mutans는 처치 8주후 까지 강력히 억제되었다(p<0.01). 2. Streptococcus sanguis는 chlorhexidine varnish처치 4주후에 일시적으로 증가하였다가(p<0.05) 8주후에 원상으로 회복되었다. 3. Streptococcus mitis, Actinomyces viscosus, Actinomyces naeslundii는 chlorhexidine varnish 처치에 의해 별 다른 변화를 나타내지 않았다.