• Journal of the Korean Magnetic Resonance Society
• /
• v.25 no.3
• /
• pp.39-44
• /
• 2021

The CRISPR-Cas system provides adaptive immunity for bacteria and archaea against invading phages and foreign plasmids. In the Class 1 CRISPR-Cas system, multi-subunit Cas proteins assemble with crRNA to bind to DNA targets. To disarm the bacterial defense system, bacteriophages evolved anti-CRISPR (Acr) proteins that actively inhibit the host CRISPR-Cas function. Here we report the backbone resonance assignments of AcrIF7 protein that inhibits the type I-F CRISPR-Cas system of Pseudomonas aeruginosa using triple-resonance nuclear magnetic resonance spectroscopy. We employed various computational methods to predict the structure and binding interface of AcrIF7, and assessed the model with experimental data. AcrIF7 binds to Cas8f protein via flexible loop regions to inhibit target DNA binding, suggesting that conformational heterogeneity is important for the Cas-Acr interaction.

• Journal of the Korean Society of Radiology
• /
• v.15 no.7
• /
• pp.1065-1072
• /
• 2021

In Computed Tomography, abdominal examination showed the highest proportion of use, and effort of reducing the radiation dose is required. Recently introduced Iterative Reconstruction(IR) is repetitive reconstruction technique of Computed Tomography. SIEMENS' IR, ADMIRE and GE's IR, ASIR-V, were used in this examination. Noise, % Contrast, and High contrast resolution were measured by using ACR phantom for image quality evaluation. In addition, CTDI_vol and DLP displayed in the CT device were used for dose evaluation. When FBP and IR were compared, stage 2 to stage 5 of ADMIRE and 10, 30, 50, 70, and 90% of ASIR-V were applied, noise could be reduced from a minimum of 0.46 to a maximum of 2.38 in ADMIRE compared to FBP, and noise from a minimum of 0.51 to a maximum of 2.5 in ASIR-V compared to FBP. Also, % Contrast and High contrast resolution of FBP and IR were no statistical difference. When IR was used for abdominal CT examination, the radiation dose of ADMIRE is reduced by 25.39% compared to the radiation dose of FBP. Also, the radiation dose of ASIR-V is reduced by 16.61% compared to the radiation dose of FBP. In conclusion, it is believed that if IR is applied during abdominal CT examination, the radiation dose can be reduced without deteriorating the image quality.

• The Korean Journal of Nuclear Medicine Technology
• /
• v.15 no.1
• /
• pp.10-16
• /
• 2011

Purpose: The partial volume effect occurs because of limit of the spatial resolution. It makes partial loss of intensity and causes SUV to be lower than it should actually be. So the purpose of this study is to calculate recovery coefficient for correcting PVE from phantom study and to compare before and after SUV correction applying to PET/CT examination. Materials and Methods: The flangeless Esser PET phantom consisting of four hot cylinders was used for this study. All of the hot cylinders were filled with FDG solution of 20.72 MBq per 1000 ml, and the phantom background was filled with FDG solution of different concentrations (33.30, 22.20, 16.65 MBq per 6440 ml) to yield H/B ratios of around 4:1, 6:1 and 8:1. Using the Biograph Truepoint 40(SIEMENS, Germany), we applied recovery coefficient method to 30 patients who were diagnosed with lung cancer after PET/CT exam. And then we analyzed and compared SUV before and after correcting partial volume effect. Results: The smaller the diameter of hot cylinder becomes, the more recovery coefficient decreased. When we applied recovery coefficient to clinical patients and compared SUV before and after correcting PVE, before the correction all lesions gave an average max SUV of 7.83. And after the correction, the average max SUV increases to 10.31. The differences in the max SUV between before and after correction were analyzed by paired t test. As a result, there were statistically significant differences (t=7.21, p=0.000). Conclusion: The SUV for quantification should be measured precisely to give consistent information of tumor uptake. But PVE is one of factors that causes SUV to be lower and to be underestimated. We can correct this PVE and calculate corrected SUV using the recovery coefficient from phantom study. And if we apply this correction method to clinical patients, we can finally assess and provide quantitative analysis more accurately.