• 대한본초학회지
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• 제22권2호
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• pp.1-12
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• 2007

Objectives : Aconitine is one of the toxic components of aconitum species. This study was carried out to evaluate gene expressions of herbal prescriptions containg aconitum species and oriental medicinal plants of aconitum species. Methods : We have measured gene expressions in the liver of aconitine, Aconitum carmichaeli DEBX., Aconitum ciliare DC. Yong Ho Whan using Sprague-Dawley rat. Gene expression in rat liver has been analyzed using codelink 10k microarray. Results : 1. Genes up-regulated over than 4 fold were 118 and down-regulated less than 4 fold were 91 in aconitine 50 ${\mu}g/kg/day$ over control. 2. Genes up-regulated of over than 4 fold were 124 and down-regulated less than 4 fold were 98 in Aconitum ciliare DC. 4g/kg/day and 169 of over than 4 fold, and 110 of less than 4 fold for Aconitum carmichaeli DEBX. 4g/kg/day, respectively. 3. Regulated genes in treatment group of Aconitum carmichaeli DEBX, Aconitum ciliare DC. and aconitine was only 2 different genes, Sulfotransferase family 4A, member 1 and Lin-7 homolog b (C. elegans). Conclusions : Gene expression profiles in liver were different among aconitine, Aconitum carmichaeli DEBX., Aconitum ciliare DC. and herbal prescription YongHo-whan. Furthermore, we can find many new genes related with effects of aconitum species.

• 한국한의학연구원논문집
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• 제12권1호
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• pp.23-38
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• 2006

The taxonomic list of specific features in external and internal shape and the pattern analysis of Aconitum carmichalei $D_{EBX}$ as the original plant of Aconiti Lateralis Radix Preparata and Aconitum cliiare Dc as the original plant of Aconiti Ciliare Tuber are as follows. 1. Aconitum carmichalei $D_{EBX}$ has tri-palmately parted leaves, petiole in lower leaves, and its ovary has short hair. Whereas Aconitum cliare Dc has $3{\sim}4$ parted leaves, long petiole, and its ovary has not hair. 2. Aconitum carmichalei $D_{EBX}$ has cylinder shape is relatively small in length and diameter, is greyish brown blacky brown in outer surface, greyish $white{\sim}dark$ gray in section. 3. According to the collection place, there is a remarkable difference in the physical shape of herbal states. Aconiti Lateralis Radix Preparate(medicated in Korea) is more transparent blacky brown color than Aconiti Lateralis Fadix Preparata(medicated in Chian). Also Black Aconi Radix(墨附片) has exodermis and White Aconi Radix(白附片) has not. 4. The internal characteristics entirely correspond to in internal shape described in the literatures, Only it is possible to discriminate between black Aconi Radix(墨附片) and White Aconi Radix(白附片) by the existence of cork layer. The classification between Aconiti Lateralis Radix Preparata and Aconiti Ciliare Tuber makes entirely Tuber makes entirely remarkable difference in the physical shape of cambium layer Namely, in shape of cambium layer the kinds of Aconiti lateralis Radix Prepala has horn-like shape and the kinds of Aconiti Ciliare Tuber has circle-like shape. 5. In the peak of examination substance in comparison to Rt of the index material diterpene alkaloid mesaconitine, aconitine, hypaconitine chromatogram Aconiti Ciliare Tuber is higher than in Aconiti Lateralis Radix Preparata This explain that the component changes after the process of medicine. 6. In the Content of mesaconitine, aconitine and hypaconitime Aconiti Ciliare Tuber is higher than Aconiti Lateralis Radix Preparata. 7. In Aconiti Lateralis Radix Preparata, aconitine, hypaconitine and mesaconitine each appears in Rf 0.46, 0.54, 0.32. But except Aconiti Ciliare Tuber the band does not appear. For the future, such results will be used as the basic source of additional research, and a far-reaching comparative study is needed to distinguish between many kinds of same genus-degree of relatedness.

• 대한약침학회지
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• 제4권2호
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• pp.87-93
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• 2001

Objectives : Through the literature on the effect of Aconiti Cliare Tuber, Radix Aconiti, we are finding out the clinical posibility and revealing the more effective to intractable disease. Methods : We inverstigated the literatures of Oriental Medicine and experimental reports about Aconiti Cliare Tuber, Radix Aconiti. Results : 1. The taste of Aconiti Cliare Tuber, Radix Aconiti is hot, sweet, bitter, warm and hot, and the effect is dehumidification, warm up and relieve the pain, so it can be used for arthritis, hemiplegia, carpopedal spasm, sciatica, cancer, numbness. 2. A toxic constituent of Aconiti Cliare Tuber, Radix Aconitiis is induced by aconitine alkaloid, develope toxic symptoms and result in death. So it needs suitabe treatment for safety. 3. It is known that the toxicopathy due to Radix Aconiti was 3-30g(dosage for adult) and Aconiti Ciliare Tuber was 1-9g. But only using aconitine alkaloid to oral feeding, the toxicopathy due to 0.2mg/kg and lethal dose is 3-4mg. So we using this for treating, we must be careful and need more varialble study about toxicopathy, lethal dose. 4. On clinical treatment, we thought Aconiti Cliare Tuber, Radix Aconiti is so effective to intractable disease after control the toxicity, it may be need variable study on toxicity and clinical effects.