• 대한미생물학회지
• /
• 제7권1호
• /
• pp.21-27
• /
• 1972

생검(生檢)한 나결절(癩結節)에서 간단(簡單)하고 효과적(效果的)인 방법(方法)인 trypsin 처리(處理)와 고속도유침(高速度遠沈)에 의(依)하여 동물접종목적(動物接種目的)에 사용(使用)할수 있는 인나균(人癩菌)의 정제(精製)에 대(對)하여 기술(記述)하였다. 본(本) 방법(方法)의 한 특징(特徵)은 그 정제과정중(精製過程中)에 재래식(在來式)인 나결절조직(癩結節組織)의 마쇄(磨碎)나 유액화조작(乳液化操作)을 전혀 포함(包含)시키지 않은 점(點)이다. 이 방법(方法)에 의(依)하여 정제(精製)된 인나균(人癩菌)을 한국산(韓國産) 다람쥐(Tamias sibiricus asiaticus, Gmelin)의 족저부(足底部) 및 이타조직내(耳朶組織內)에 접종(接種)하였던 바 균접종(菌接種) 8 및 12개월(個月)에 이르러 균증식양상(菌增殖樣狀)이 야기(惹起)됨이 관찰(觀察)되었다.

• Tuberculosis and Respiratory Diseases
• /
• 제64권4호
• /
• pp.293-297
• /
• 2008

M. fortuitum은 호흡기 검체에서 동정되는 경우 대부분 집락화나 일시적인 감염으로 여겨지고 있고, 다른 NTM 폐질환처럼 장기간의 항생제 치료가 필요한 경우는 드물다. 저자들은 객담 항산균 도말과 배양검사에서 강양성을 보이면서, 임상증상과 방사선의 악화소견을 보여 장기간의 경구용 항생제 치료를 실시한 M. fortuitum 폐질환 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다.

• Tuberculosis and Respiratory Diseases
• /
• 제62권6호
• /
• pp.531-535
• /
• 2007

식도결핵은 매우 드문 질환으로 원발성으로 발생하는 경우는 거의 없고 발생한다 하더라도 대부분 속발성으로 발생한다. 가장 흔한 원인으로는 종격동 림프절의 침범에 의해 인접해 있는 식도로 전파되는 것으로 이러한 경우 식도-종격동 누공 등의 합병증이 발생할 수가 있다. 저자들은 식도결핵 및 이의 합병증으로 식도-종격동 누공이 형성된 환자를 항결핵제 투여만으로 식도결핵의 호전과 누공의 폐쇄를 경험하였기에 보고하는 바이다.

• Tuberculosis and Respiratory Diseases
• /
• 제75권4호
• /
• pp.150-156
• /
• 2013

Background: Thoracoscopic pleural biopsy is often required for rapid and confirmative diagnosis in patients with suspected pleural tuberculosis (PL-TB). However, this method is more invasive and costly than its alternatives. Therefore, we evaluated the clinical utility of the chest computed tomography (CT)-based bronchial aspirate (BA) TB-polymerase chain reaction (PCR) test in such patients. Methods: Bronchoscopic evaluation was performed in 54 patients with presumptive PL-TB through diagnostic thoracentesis but without a positive result of sputum acid-fast bacilli (AFB) smear, pleural fluid AFB smear, or pleural fluid TB-PCR test. Diagnostic yields of BA were evaluated according to the characteristics of parenchymal lesions on chest CT. Results: Chest radiograph and CT revealed parenchymal lesions in 25 (46%) and 40 (74%) of 54 patients, respectively. In cases with an absence of parenchymal lesions on chest CT, the bronchoscopic approach had no diagnostic benefit. BA TB-PCR test was positive in 21 out of 22 (95%) patients with early-positive results. Among BA results from 20 (37%) patients with patchy consolidative CT findings, eight (40%) were AFB smear-positive, 18 (90%) were TB-PCR-positive, and 19 (95%) were culture-positive. Conclusion: The BA TB-PCR test seems to be a satisfactory diagnostic modality in patients with suspected PL-TB and patchy consolidative CT findings. For rapid and confirmative diagnosis in these patients, the bronchoscopic approach with TB-PCR may be preferable to the thoracoscopy.

• Pediatric Infection and Vaccine
• /
• 제13권2호
• /
• pp.174-179
• /
• 2006

국내에서는 아직도 소아연령에서 결핵 감염이 적지 않게 존재하기 때문에 폐외 결핵 환자가 발생될 위험도 지속적으로 존재한다. 이런 측면에서 폐외 결핵의 하나인 골관절 결핵은 매우 드물기는 하나, 관절염을 주소로 내원한 환자의 원인으로 고려해 볼만하다. 나이 어린 소아에서는 증상과 징후가 비특이적이기 때문에 오진이나 진단 지연이 우려되므로, 항생제 치료에 잘 반응하지 않는 관절염 환자에서는 결핵성 골관절염에 대한 진단과 치료를 시도하는 것이 중요하다.

• Journal of Chest Surgery
• /
• 제33권8호
• /
• pp.669-675
• /
• 2000

Background: Tuberculous pleurisy is the leading cause of pleural effusion in Korea. And differential diagnosis of tuberculous pleurisy with other cause is clinically very important. Traditional diagnostic methods such as routine analysis of pleural fluid, staining for acid-fast bacilli or pleural biopsy have major inherent limitaion. This study was designed to evaluate the significance of pleural fluid polymerase chain reaction(PCR) and adenosine deaminase (ADA) activity in early diagnosis of tuberculous pleurisy. Material and Method: Between March 1996 and July 1997, 198 patients with pleural effusion reviewed retrospectively. The study group included 112 cases with tuberculous effusion and 86 cases with non-tuberculous effusions, whose diagnoses were confirmed by pleural biopsy, microbiological methods, or cytology. We compared the results of PCR and pleural fluid levels of ADA between tuberculous and non-tuberculous effusions. Result: Mean age was 47.54$\pm$19.52 years(range 2 to 85 years). The positive rate of PCR was significantly higher in tuberculous group than non-tuberculous group(p<0.05). The sensitivty, specificity, positive predictive value(PPV), and negative predictive value(NPV) for PCR were 31.7, 90.9, 83.0, and 48.8%, respectively. Mean ADA activity was significantly higher in tuberculous group than non-tuberculous group(83.2 U/L vs 49.8 U/L)(p<0.05). With diagnostic thresholds of 40 U/L, the sensitivity, specificity, PPV, and NPV of ADA for tuberculosis were 75.9, 70.9, 77.3, and 69.3% respectively. At a level of 70 U/L, the sensitivity, specificity, PPV, and NPV of ADA for tuberculosis were 70.1, 75.9, 82.9, and 60.3% respectively. Conclusion: PCR is very highly specific, but less sensitive methods in diagnosis of tuberculous pleurisy. But ADA level of pleural fluid has acceptable sensitivity and specificity in diagnosis of tuberculous pleurisy. ADA activity is more useful test in the evaluation of pleural effusions.

• 대한미생물학회지
• /
• 제18권1호
• /
• pp.99-110
• /
• 1983

저자 등은 결핵으로 인해 생긴 공동을 가진 4명의 남자 환자에 있어서 성공적인 결핵화학요법으로 결핵은 치유되어 객담검사에서 결핵균은 더이상 검출되지 않았으나, 계속되는 각혈로 진균검사를 실시한 결과 Pseudallerscheria boydii(불완전세대 Scedosporium apiospermum)에 의한 진균종(眞菌腫)임이 밝혀졌다. 여러 차례의 객담검사로부터 6개월 또는 2년 이상 계속 동일한 균종이 불리 배양되었고 그리고 그들의 혈청은 P. boydii 배양여액 항원과 immunodiffusion test를 실시한 결과 precipitin bands를 형성하였다. 4환자의 흉부 X-선 사진상에는 뚜렷한 진균종음영을 볼 수 없었다. 2명의 환자 객담에서는 P. boydii와 더불어 Candida albicans와 Aspergillus fumigatus가 계속해서 분리 배양되었고 그리고 그들의 혈청에서 그러한 진균들의 항원에 대한 침강항체를 검충할 수 있었다. 3명의 환자는 폐기능이 진균종 제거수술을 허용하지 않았고 1명의 환자는 수술을 거부하였다.

• Tuberculosis and Respiratory Diseases
• /
• 제39권4호
• /
• pp.355-360
• /
• 1992

저자들은 57세 남자환자에서 전형적인 Nocardiosis의 임상상을 보이고 객담 및 피부 농양에서 Nocardia asteroides가 분리 동정된 1예를 경험하였기에 이를 문헌고찰과 함께 보고하는 바이다.

• Journal of Microbiology and Biotechnology
• /
• 제24권7호
• /
• pp.1004-1007
• /
• 2014

Culture is the gold standard for diagnosis of tuberculosis, but it takes 6 to 8 weeks to confirm the result. This issue is complemented by the detection method using polymerase chain reaction, which is now widely used in a routine microbiology laboratory. In this study, we evaluated the performance of the Seegene Anyplex TB PCR to assess its diagnostic sensitivity and specificity, and compared its results with the Roche Cobas TaqMan MTB PCR, one of the most widely used assays in the world. Five university hospitals located in the Chungcheong area in South Korea participated in the study. A total of 1,167 respiratory specimens ordered for acid-fast bacilli staining and culture were collected for four months, analyzed via the Seegene Anyplex TB PCR, and its results were compared with the Roche Cobas TaqMan MTB PCR. For detection of Mycobacterium tuberculosis, the diagnostic sensitivity and specificity of the Anyplex TB PCR were 87.5% and 98.2% respectively, whereas those of the Cobas TaqMan were 92.0% and 98.0% respectively (p value > 0.05). For smear-positive specimens, the sensitivity of the Anyplex TB PCR was 95.2%, which was exactly the same as that of the Cobas TaqMan. For smear-negative specimens, the sensitivity of the Anyplex TB PCR was 69.2%, whereas that of the Cobas TaqMan TB PCR was 84.6%. For detection of MTB, the Seegene Anyplex TB PCR showed excellent diagnostic performance, and high sensitivity and specificity, which were comparable to the Roche Cobas TaqMan MTB PCR. In conclusion, the Anyplex TB PCR can be a useful diagnostic tool for the early detection of tuberculosis in clinical laboratories.

• 대한두개안면성형외과학회지
• /
• 제12권1호
• /
• pp.71-74
• /
• 2011

Purpose: Sarcoidosis is a systemic noncaseating granulomatous disease of an unknown origin, and can involve any organ including the skin. The infiltration of sarcoid granuloma in an old cutaneous scar is an uncommon cutaneous manifestation of sarcoidosis. This paper reports a 35-year old female who presented with cutanesous nodules in previous facial scars. Methods: A 35-year-old female presented with cutaneous nodules for 2 months in previous scars of the forehead and lower lip that she had acquired in the childhood. An excisional biopsy of the lower lip mass, serologic examinations and radiologic studies were performed. Results: The excisional biopsy revealed noncaseating granulomas consistent with sarcoidosis. Chest CT revealed both hilar and paratracheal lymphadenopathy with nodular densities in both lung fields. Routine laboratory tests, serologic tests, serum angiotensin converting enzyme level, sputum for acid-fast bacilli, ophthalmoscopic examination, TB-PCR and NTM-PCR showed normal findings. Therefore, the patient was diagnosed with sarcoidosis. The patient refused fiberoptic bronchoscopy and medication with oral steroid. Currently, the clinical manifestation and progress are being monitored closely, and treatment is expected to start with oral steroid according to the progress. Conclusion: For the proper management of cutaneous sarcoidosis, particularly scar sarcoidosis, plastic surgeons should be aware of the many clinical and histopathological features of sarcoidosis and recommend a systemic evaluation for early diagnosis and proper treatment.