• 농업과학연구
• /
• 제8권1호
• /
• pp.108-116
• /
• 1981

밀감과피(果皮)를 이용(利用)한 주정초(酒精醋)를 생산(生産)하는데에 필요(必要)한 기초질료(基礎質料)를 얻기위(爲)하여 실험(實驗)하였으며 다음과 같은 결과(結果)를 얻었다. 1. 과피수율(果皮收率)은 29.0%였다. 2. 초산(醋酸)발효 배지중(培地中)의 밀감과피즙(果皮汁) 함유량(含有量)은 25%내외(內外)가 적당(適當)하였다. 3. 배지중(培地中)의 밀감 과피즙(果皮汁) 농도(濃度)가 70% 이상인 경우에는 초산(醋酸)발효가 저해되었으며 밀감과 피즙 농도가 90%인 경우에는 밀감 과피즙(果皮汁) 농도(濃度) 25%에 비(比)하여 초산생성(醋酸生成)의 최고산도가 약(約) 1% 저하되었다. 4. 초산(醋酸)발효 배지중(培地中)의 밀감과피즙(果皮汁) 농도(濃度)를 25%로 하여 통기(通氣) 발효를 할 경우 평균(平均) 산생성속도(酸生成速度)는 0.062g/100ml.hr. 대수기의 산생성속도(酸生成速度)는 0.15g/100ml.hr., 이론치에 대한 실제치의 산화수율(酸化收率)은 91.4%였다. 5. 초산(醋酸) 발효액에서 oxalate, malate 등(等)이 검출 되었다. 6. 밀감 과피즙(果皮汁) 함량 25% 배지(培地)를 사용(使用)할 경우 생성(生成)된 식초(食醋)의 품질(品質)은 상당히 양호(良好)하였다.

• 한국미생물·생명공학회지
• /
• 제7권3호
• /
• pp.157-164
• /
• 1979

Aspergillus 속균중 Asp. awamori U-3가 acid protease의 생산능이 우수함으로 효소생산에 미치는 배양조건 및 조효소의 특성을 검토하고 다음과 같은 결과를 얻었다. 1. 최적배양온도는 3$0^{\circ}C$, 최적배양시간은 72 시간, 최적첨수량은 wheat bran medium 에서는 100~120 %, defatted rice bran medium 에서는 100~130%이었다. 2. 명종 성분중 wheat bran medium에는 KN $O_3$, glutamic acid, glucose 가, defatted rice bran me-dium 에는 KN $O_3$, (N $H_4$)$_2$S $O_4$, glucose, lactose, K $H_2$P $O_4$ 및 MgC $l_2$의 첨가가 특히 효과적이었으며, wheat bran medium 에서 가장 효과적인 glucose, KN $O_3$와 glutamic acid의 최적 첨가농도는 명명 3.0~4.0%, 0.2~0.4%, 1.0 %이었다. 3. 본효소의 작용 최적 pH는 2.4, 최적온도는 45$^{\circ}C$내외, 안정 pH 범위는 2.0~5.0이고, 5$0^{\circ}C$ 이하에서는안정하나 그 이상에서는 급격하게 불활성화 되었다. 4. 본효소에 대한 명종 무기염류의 내열효과를 검토한바 CaC $l_2$와 CaS $O_4$ 가 약간의 내열효과를 나타내었다. 5. 내열제를 첨가하고 6$0^{\circ}C$에서 10~30 분간 처리할때 20분 이상에서는 효소의 잔존활성이 크게 감소되었고, 내열제로서 CaC $l_2$와 CaS $O_4$ 를 첨가할 때 다같이 8$0^{\circ}C$에서는 내열효과는 거의 없었다.

• Applied Biological Chemistry
• /
• 제29권1호
• /
• pp.3-9
• /
• 1986

Alkane 효모의 유성(有性) 생활환(生活環)을 이용한 유전적 해석과 대사경로(代謝經路)에 관한 기초자료를 얻고자 토양과 오염된 치이즈에서 citric acid의 생산성이 높은 균주를 분리하여 동정하고 교잡(交雜)에 의하여 2배체(倍體) 체화(體化)하였으며, 이들의 유전적, 생화학적 및 효소학적 특성을 검토하여 다음과 같은 결과를 얻었다. Citric acid와 isocitric acid를 생산하는 CJ2, CJ7과 CJ8 균주는 Saccharomycopsis lipolytica의 교배형(交配型) A, B와 B로 각각 동정되어 교잡(交雜)과 포자형성(胞子形成)에 의하여 얻은 배수체(倍數體)의 감수분열(減數分裂) 분리를 행한바 재조합형(再組合型) 자손이 얻어졌다. 모든 균주는 glucose배지보다 n-hexadecane배지에서 재친(再親) 1배체(倍體)보다 2배체(倍體)가 citric acid를 많이 생산하였고 isocitrate Iyase의 비활성(比活性)은 n-hexadecane배지에서 생육한 세포가 glucose배지에서 생육한 세포보다 $15{\sim}20$배 높았지만 citrate synthetase의 비활성(比活性)은 탄소원에 의하여는 거의 영향을 받지 않았다. 또한 이들 효소의 비활성(比活性)과 citric acid의 생산 사이에는 균주와 배수성에 관계없이 상관관계가 적었다.

• 한국응용과학기술학회지
• /
• 제32권1호
• /
• pp.93-99
• /
• 2015

본 연구는 유채 꽃가루 분말의 영양성분 분석과 파쇄한 꽃가루 분말을 이용하여 효모 배양용 액체배지와 고체배지를 제조하여 효모 배양효율을 분석하였다. 유채 꽃가루 분말(100 g)은 탄수화물 58.9 g, 단백질 20.8 g, 지방 4.1 g, 회분 2.5 g 및 수분 13.7 g으로 구성되었다. 당질 중에서는 Fructose(13.7 g), Glucose(11.1 g)와 Sucrose(6.6 g), 미네랄 중에서는 K(606.7 mg)와 P(603.3 mg) 함향이 높았다. 유리 아미노산은 Glutamic acid(2,482.4 mg), Aspartic acid(2,136.5 mg), Lysine(1,648.3 mg), 및 Leucine(1,631.1 mg) 순으로 많았다. 유채 꽃가루 분말을 단독 또는 NaCl을 첨가하여 액체배지 및 고체배지 제조하고 효모를 배양한 결과, 꽃가루 분말이 15 g/L 이상 함유된 액체배지는 효모 배양 후 건물중이 YPD 배지(대조구)에 비해 많았고, 꽃가루 분말의 농도 의존적으로 효모 건물중이 증가하였다. 또한 액체배지에 NaCl을 1~20 g/L 추가 시 배양된 효모의 건물중이 한층 더 증가하였다. 유채 꽃가루로 제조한 고체배지를 이용하여 효모를 배양(48시간)한 결과, 콜로니(colonies) YPD 배지와 유사하게 잘 자랐다.

• International Journal of Internet, Broadcasting and Communication
• /
• 제13권2호
• /
• pp.82-92
• /
• 2021

Purpose: It has been reported that the diversity and abundance of microbes in the vagina decrease due to the use of antimicrobial agents, and the high recurrence rate of female vaginitis due to this suggests that a new treatment is needed. Methods: In the experiment, we detected that 10% potassium sorbate solution, 1% eucalyptus oil solution, 1% tea tree oil solution, 400 µL/10 mL grapefruit seed extract solution, 100% lactic acid, 10% acetic acid solution, and 10% lactic acid solution were prepared and used. After adjusting the pH to 4, 5, and 6 with lactic acid and acetic acid in the mixed culture medium, each bacterium was inoculated into the medium and incubated for 72 h at 35℃. Incubate and 0 h each. 24 h. 48 h. The number of bacteria was measured after 72 h. Results: In the mixed culture test between lactic acid bacteria and pathogenic microorganisms, lactic acid bacteria showed good results at pH 5-5.5. Potassium sorbate, which has varying antibacterial activity based on the pH, killed pathogenic bacteria and allowed lactic acid bacteria to survive at pH 5.5. Conclusion: The formulation ratio obtained through this study could be used for the development of a feminine cleanser that can be used as a substitute for antibacterial agents. Further, the findings of this study may be able to solve the problem of antimicrobial resistance in the future.

• 한국축산식품학회지
• /
• 제30권1호
• /
• pp.154-162
• /
• 2010

This study was carried out to compare the efficacy and selectivity of TOS and BS media for enumeration of bifidobacteria in commercial fermented milk products. First, bifidobacteria was isolated from 20 fermented milk products, and all isolated bifidobacteria were identified by genomic technology as Bifidobacterium lactis. The two media significantly differed from each other with regard to the recovery of B. lactis, that is, the recovery of this organism was as much as 6 logs lower on BS medium than on TOS. When the concentration of BS solution (mixture of paromomycin sulfate, neomycin, sodium propionate, and lithium chloride) used in BS medium was reduced to 50% (BS50), a relatively high percentage recovery of bifidobacteria from pure cultures was achieved. Susceptibility tests to antibiotics and tests for selective agents for the isolated bifidobacteria and lactic acid bacteria were conducted. The BS solution inhibited some lactic acid bacteria and Bifidobacterium species, while mupirocin (MU) suppressed the growth of all tested lactic acid bacteria but not Bifidobacterium. As compared with BS50 medium, TOS with or without MU showed good bifidobacteria recovery and readily distinguishable colonies; in particular, TOS supplemented with MU had a high selectivity for bifidobacteria. In conclusion, all results suggested that TOS medium with or without MU was found to be suitable for selective enumeration of bifidobacteria from mixed cultures in fermented milk, and better in that capacity than BS medium.

• The Plant Pathology Journal
• /
• 제16권4호
• /
• pp.236-241
• /
• 2000

A selective agar medium was developed and tested for the isolation of Acidovorax avenae subsp. avenae, the causal bacterial pathogen of bacterial brown stripe, from rice seeds. The new selective agar medium, designated sorbitol pyroglutamic acid agar (SPA) medium, contained 0.5 g of $K_2$HPO$_4$, 3.0 g of Na$_2$HPO$_4$, 2.0 g of D-sorbitol, 0.2 g of L-pyroglutamic acid, 10.0 $m\ell$ of tween 80, 40.0 mg of victoria blue B, 15.0 g of agar, 150.0 mg of ampicillin and 25.0 mg of vancomycin per litter. Colonies of A. avenae subsp. avenae on SPA medium were smooth, round, convex, shiny, blue and 1.5-2.0 mm in diameter 4 days after incubation at 28$^{\circ}C$. Blue colored colony having dark blue zone was typical type of A. avenae subsp. avenae colonies on the medium. Mean recovery of 8 isolates of A. avenae subsp. avenae on the selective SPA medium was 95.8% in comparison to that on KB medium. The saprophytic bacteria were reduced to 97.9% on SPA medium compared to those on KB medium. Most of other rice seedborne bacteria as well as reported pathogenic bacteria were failed to grow on SPA medium. This medium was highly selective for recovering A. avenae subsp. avenae from rice seed samples, and it could be used to enhance the recovery of this bacterium from rice seed samples, which may be contaminated with large numbers of competing microorganisms.

• 한국환경보건학회지
• /
• 제24권3호
• /
• pp.54-62
• /
• 1998

20 bacterial strains capable of growing on phenol minimal medium were isolated from soil and wastewater by the enrichment culture technique, and among them, one isolate which was the best in the cell growth was selected and identified as Bacillus sp. SH3 by its characteristics. Strain SH3 could grow with phenol as the sole carbon source up to 15 mM, but did not grow in minimal medium containing above 20 mM of phenol. The optimal conditions of temperature and initial pH for growth and phenol degradation were 30$^{\circ}$C and 7.5, respectively. This strain could grow on various aromatic compounds such as catechol, protocatechuic acid, gentisic acid, o-, m-, p-cresol, benzoic acid, p-hydroxybenzoic acid, anthranilic acid, phenyl acetate and pentachlorophenol, and the growth-limiting log P value of strain SH3 on organic solvents was 3.1. In batch culture, strain SH3 degraded 97% of 10 mM phenol in 48 hours. In continuous culture under the conditions of 20 mM of influent phenol concentration and 0.050 hr$^{-1}$ of dilution rate, the treatment rate of phenol was 94%.

• Journal of Nutrition and Health
• /
• 제22권3호
• /
• pp.159-166
• /
• 1989

The effect of blanching time and power settings of microwave oven on the ascorbic acid retention in seven kinds of vegetables were investigated. The vegetable were blanched for 120 or 180 sec. at three different power setting, 650 watt(high power), 500 watt(medium power) and 160 watt(low power). The retentions of ascorbic acid in cabbage, garland chrysanthemum, mungbean sprout, amaranth and carrot were higher when they were blanched at the high power than those blanced at the lower power stettings. Blanching of spinach and yul-moo(small korean radish) showed that the vitamin was more retained by the medium power heating. Blanching at the low power revealed that the ascorbic acid retention was reduced remarkably as the blanching time increased. From the scoring difference tests the 10-panel members indicated that the texture of three-tested vegetables was more acceptable when they were blanched at the high power setting.

• Journal of Microbiology and Biotechnology
• /
• 제1권2호
• /
• pp.126-129
• /
• 1991

In order to produce ${\gamma}-linolenic$ acid by Mucor sp. KCTC 8405P. the fungus was cultivated in fed-batch culture with two phases. i.e., growth in yeast-like form and induction to hyphal growth by pH shift of the culture medium during cultivation. The synchronous growth of the fungus into the appropriate sizes was important for the high density cell culture of this dimorphic fungus. Dissolved oxygen concentration in the medium did not affect degree of unsaturation of fatty acids and ${\gamma}-linolenic$ acid content. Under the culture conditions applied in this experiment. the fungus was found to produce 100 g/l dry mycelia containing 40% of the lipids, where ${\gamma}-linolenic$ acid comprised about 9% of the total extractable fatty acids.