• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.3
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• pp.145-150
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• 2007

This experiment was carried out to breed the cold-tolerant variety of Italian ryegrass (Lolium multiflorum Lam.) in Grassland and Forage Crops Division, National Institute of Animal Science, RDA, Cheonan from 1999 to 2005. New variety, 'Kospeed' is a diploid variety with green in leaf color, and has semi-erect and erect growth habit in late autumn and early spring, respectively. 'Kospeed' was on 1 May in heading date as a early-maturing variety. Also 'Kospeed' was wider by 0.2mm in flag leaf width, longer by 1cm in flag leaf length and shorter by 1cm in plant length than those of 'Florida 80', respectively. Stem width of 'Kospeed' was similar to that of 'Florida 80' and 'Hwasan 101'. Cold tolerance of 'Kospeed' was better than that of 'Florida 80'. Dry matter (DM) yield (13,257kg/ha) of 'Kospeed' was similar to that of 'Florida 80'. In vitro dry matter digestibility (IVDMD) and total digestible nutrient (TDN) of 'Kospeed' were 73.2% and 65.3% which are 2.1% and 1.7% higher than those of 'Florida 80', respectively. Acid detergent fiber(ADF) and neutral detergent fiber (NDF) of 'Kospeed' were 29.1% and 53.1% which are 2.1% and 2.3% lower than those of 'Florida 80', respectively.

• Korean Journal of Microbiology
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• v.30 no.6
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• pp.564-569
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• 1992

A green sulfur bacterium, Chlorobium limicola f. thiosulfatophilum NCIB 8327, was grown in modified Pfennig's medium including glu1amate as a nitrogen source. Glutamine synthetase was isolated through a series of ultracentrifugation. DEAE-Sepharose CL-6B ion exchange chromatography. Sephacryl S-300 gel permeation chromatography, and preparative HPLC. The recovery and purification fold of the enzyme were 2% and 46.3. respectively. The isolated enzyme was homogeneous on UV-Visible spectrum and polyacrylamide gel electrophoretogram. The relative molecular mass of the native enzyme was estimated to be 280,000 by gel permeation chromatography. The enzyme consisted of ten subunits with relative similar molecular mass. 30.000. which was estimated by SDS-polyacrylamide gel electrophoresis. The optimal temperature and pH of the enzyme were $30^{\circ}C$ and 7.0. Km values were 27.9 mM for L-glutamine and 0.92 mM for hydroxylamine-HCr. The enzyme activity was inhibited by alanine. glycine. and tryptophan considerably, but was not affected by asparagine, lysine. leucine. and valine.

• Journal of The Korean Society of Grassland and Forage Science
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• v.35 no.1
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• pp.31-35
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• 2015

This experiment was carried out to breed a very early-maturing variety of Italian ryegrass (Lolium multiflorum Lam.) in the Grassland and Forage Crops Division, National Institute of Animal Science, RDA, Cheonan, Korea from 2012 to 2014. New variety "Greenfarm3ho" is a diploid variety with a green leaf color, a semi-erect growth habit in late autumn/fall, and an erect growth habit in early spring. With a heading date of April 27, Greenfarm3ho was categorized as an early-maturing variety. Compared with the "Greenfarm" control variety, Greenfarm3ho's flag-leaf width was narrower by 0.5 mm, its flag-leaf length was longer by 4.4 cm, and its plant length was longer by 3 cm; additionally, Greenfarm3ho's stem thickness was 0.1 mm thicker and it showed winter hardiness. The dry matter (DM) yield (8,976 kg/ha) of Greenfarm3ho was similar to that of Greenfarm. The in vitro dry matter digestibility (IVDMD), total digestible nutrient (TDN), and crude protein (CP) of Greenfarm3ho were 70.0%, 64.1%, and 1.0%, respectively, which are higher than those of Greenfarm by 2.9%, 2.0%, and 1.4%, respectively. The acid detergent fiber (ADF) and neutral detergent fiber (NDF) content of Greenfarm3ho were 31.4% and 53.3%, respectively, which are lower than those of Greenfarm by 2.5% and 3.0%, respectively.

• Journal of The Korean Society of Grassland and Forage Science
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• v.41 no.1
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• pp.10-15
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• 2021

'Gwangyoung', a winter forage triticale cultivar (X Triticosecale Wittmack), was developed at the Department of Rice and Winter Cereal Crop, NICS, RDA in 2018. The cultivar 'Gwangyoung' has leaves of wide width, medium length, and green color, and spikes of medium length and yellowish-brown color, and a large grain of yellowish-brown color. The heading date of the cultivar 'Gwangyoung' was April 22 which was similar to check cultivar 'Shinyoung'. Its tolerance or resistance to cold, lodging, wet injury powdery mildew, and leaf rust were also similar to those of the check cultivar. The leaf blade ratio of 'Gwangyoung' (27.5%) was higher than that of 'Shinyoung' (21.2%). The average forage fresh and dry matter yield of cultivar 'Gwangyoung' at milk-ripe stages were 50.0 and 17.6 MT ha-1, respectively, which were higher than those (47.7 and 17.1 MT ha-1) of the check cultivar. The silage quality of 'Gwangyoung' was lower than that of the check cultivar 'Shinyoung' in crude protein content (5.3%) and total digestible nutrients (61.3%), while was higher than the check cultivar in neutral detergent fiber (58.2%) and acid detergent fiber (34.9%). 'Gwangyoung' showed the silage of 1 grade and a grain yield of 6.03 MT ha-1.

• Journal of Food Hygiene and Safety
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• v.27 no.2
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• pp.169-175
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• 2012

Five kinds of selective media, such as mannitol salt agar (MSA), Baird-Parker agar (BPA), Baird-Parker supplemented with rabbit plasma fibrinogen (BPA+RPF), CHROMagar Staphylococcus aureus (CSA), and Petrifilm Staph Express count system (Petrifilm), were compared to recommend the optimum selective media for isolation of Staphylococcus aureus from agricultural products. Seventy four target and non target bacteria were inoculated on five selective media to analyze sensitivity and specificity. In the recovery test of injured S. aureus cells, S. aureus was exposed to acid (1% lactic acid for 10 min), heat ($60^{\circ}C$ for 90s), and cold ($-20^{\circ}C$ for 1h) conditions. And artificially contaminated agricultural products (iceberg lettuce, green pepper, and cherry tomato) was enumerated on five selective media. The sensitivity of BPA+RPF, CSA, Petrifilm, MSA, and BPA were 100%, 100%, 100%, 90.5%, 90.5%, respectively. In addition, the specificity of BPA+RPF, CSA, MSA, BPA and Petrifilm were 100%, 100%, 84.6%, 75.0%, 67.3%, respectively. However, no difference among five selective media was observed in recovery on injured S. aureus cell and enumeration from agricultural products. This results suggest that BPA+RPF and CSA are the optimum media for detection of S. aureus from agricultural products.

• ALGAE
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• v.33 no.1
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• pp.109-125
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• 2018

Turkish towel (Chondracanthus exasperatus), Pacific dulse (Palmaria mollis, also known as Red ribbon seaweed), and sea lettuce (Ulva spp.) were cultivated in a land-based intensive culture system at the Manchester Research Station, USA from August 2013 to September 2014. Macroalgae were grown in tumble-aerated tanks, harvested bimonthly for seasonal growth calculations, and analyzed for protein, lipid, ash, and amino acid content. Growth rate of all three species exhibited a similar pattern, with the highest specific growth rates occurring during the summer months (Turkish towel: 7.8%, Pacific dulse: 8.2%, and sea lettuce: 6.2%). Growth of all three species was lowest around winter solstice; with negative growth only observed in sea lettuce. On a dry weight basis significant differences in protein content existed between the three species with highest values for sea lettuce ($29.5{\pm}1.4%$). Lipid content varied between species (0.95-2.78%) with significantly higher lipid observed in sea lettuce (0.58-4.82%). No significant differences were detected on a seasonal basis among each species. Essential amino acids accounted for $43{\pm}0.9$ to $47{\pm}1.2%$ of total amino acids with Turkish towel having the highest value. Turkish towel had a significantly higher taurine level ($0.82{\pm}0.27$) than the other macroalgae. The levels of persistent organic pollutants and heavy metals were low. The estimated annual product of the three species ranged from 50- to $70-mt\;dry\;weight\;ha^{-1}\;y^{-1}$, significantly higher than conventional crops. Land-based culture of these species can produce year-round harvest, consistent product quality, and low contaminant levels.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.5
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• pp.699-707
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• 2002

Diets with foliage of Flemingia (Flemingia macrophylla) or Jackfruit (Artocapus heterophyllus were fed to goats with the objective to study nitrogen (N) balance and effect of a daily supplementation of polyethylene glycol (PEG) on intake and digestion. In experiment 1, three male Alpine${\times}$Jamnapary goats with initial weights varying from 26.9 to 27.7 kg were used in a $3{\times}3$ Latin square design in the dry season. Three Alpine${\times}$Bachthao crosses, 15.3-16.7 kg, were used in the same design in the wet season. The three diets were based on chopped whole sugar cane complemented with the two green foliages, Jackfruit and Flemingia, or soybean meal (SBM). The level of dry matter (DM) offered was 4% of body weight (BW), 2.7% as foliage and 1.3% as chopped whole sugar cane. The amount of SBM offered was calculated to give the same amount of crude protein (CP) as the foliages. Each experimental period lasted 32 days (14 days for adaptation, 7 days for collection and 10 days for rest). Feed intake, apparent digestibility of DM, organic matter (OM), CP, neutral detergent fiber (NDF) and acid detergent fiber (ADF) and retained nitrogen (N) were measured by total faecal and urine collection. In experiment 2, four male goats (Alpine${\times}$Jamnapary) with initial weights from 17.1 to 23.1 kg were used in a $4{\times}4$ Latin square design. The four treatments were Jackfruit or Flemingia with or without addition of PEG, which was fed at a level of 5 g/goat and day by mixing with a small amount of rice bran. Each experimental period lasted 15 days (8 days for adaptation, 7 days for collection). Measurements were done as in experiment 1. The DM digestibility was highest (65.9-74.3%) for goats fed the SBM diet in both the dry and wet season. The DM digestibility of goats fed the Jackfruit and the Flemingia diets was similar in both the dry (58.6-59.2% respectively) and the wet season (53.9-56.1% respectively). The CP digestibility was highest (73.0-73.6%) for the SBM diet followed by the Jackfruit diet (47.0-38.5%) and was lowest (36.8-30.0%) for the Flemingia diet in both dry and wet seasons, respectively. The NDF digestibility was low for both the Jackfruit (36.4%) and Flemingia (38.0%) diets in the wet season. All diets resulted in a positive N balance. The N retention was highest (0.465-0.604 g/kg $W^{0.75}$) in the SBM diets and lowest (0.012-0.250 g/kg $W^{0.75}$) in the Flemingia diet. Addition of PEG had no effect on feed intake for any of the diets. PEG added in the Flemingia diet had a positive effect only on NDF digestibility, but the digestibility of the Jackfruit diet was significantly increased. Supplementation with PEG reduced digestibility and N retention of Flemingia, possibly because of the low tannin level, but increased digestibility and N retention for Jackfruit foliage.

• Journal of Animal Science and Technology
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• v.48 no.3
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• pp.345-352
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• 2006

Korean native cattle (Hanwoo) have a good capacity to produce heavily marbled meat of high value. The intramuscular fat in Hanwoo is known to be deposit from 12 months of age by degree of slightly visible and significantly developed in 28 months of age. Lipogenesis gene expression profiling in longissimus dorsi at early and late fattening stage will be helpful to understand the mechanism of intramuscular fat deposition in skeletal muscle. Therefore, we analysed the gene expression patterns of six genes related lipid metabolism (FABP4, GLUT4, LPL, ACC, ACL and SCD) between early and late fattening stage. The mRNA expression of FABP4 at late fattening stage (27 months old) was higher about 3.0 fold than at early fattening stage (12 months old) in each three individuals of Hanwoo. However, GLUT4 mRNA expression was not different at late fattening stage compared with at early fattening stage. On the other hand, The expression patterns of LPL, ACC, ACL and SCD genes related lipid metabolism were significantly over-expressed about 3.5 fold, 2.7 fold, 3.7 fold and 7.5 fold at late fattening stage, respectively. Thus, these results suggested that lipogenesis in skeletal muscle at late fattening stage is due to increasing uptake of fatty acid by FABP4 and lipogenesis gene expression such as LPL, ACC, ACL and SCD.

• Journal of the Korea Organic Resources Recycling Association
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• v.11 no.1
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• pp.91-91
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• 2003

시험은 대조구 0%와 남은 음식물에 Protein의 요구량 수준을 각각 높인 10%, 20%, 30%, 40%, 대조구에 녹차 부산물(GTB) 첨가구 1.0%, 남은 음식물 30% + 녹차 부산물 (GTB) 1.0%로 총 7처리 4반복으로 하였으며, 반복당 6수씩 개체별로 사양 실험을 실시하고, 산란율, 난중, 난각두께, 난황색, Thiobarbituric acid (TBA) 및 관능검사 등을 측정한 결과는 다음과 같다. 1. 산란율은 남은 음식물 10%, 20% 및 40% 첨가구에서 각각 82.65%, 81.54% 및 82.27%로 높게 나타났으나 남은 음식물 30%첨가구는 75.34%로 낮게 나타났으며 통계적 유의차를 보였다 (P<0.05). 대조구가 72.87%가 가장 낮게 나타났으며 (P<0.05), 주령별로는 주령이 증가할수록 산란율이 다소 감소하는 경향을 보였다. 2. 난중은 전 처리구중 가장 높은 경향을 보인 것은 남은 음식물 30% + GTB 1%처리구가 63.78로 높은 경향을 보였다. 가장 낮은 난중은 남은 음식물 10%처리구가 60.02로 나타났다 (P<0.05). 3. 산란량은 전 처리구에서 남은 음식물 30% + GTB 1%가 52.53g으로 높게 나타났고, 가장 낮은 산란량을 보인 것은 대조구가 45.32g으로 가장 낮게 나타났으며 통계적 유의차를 보였다 (P<0.05). 4. 사료 요구율은 남은 음식물 20%첨가구가 2.86으로 높게 나타났으며, 낮은 요구율은 남은 음식물 10%첨가구가 2.47으로 낮게 나타났으며 통계적 유의차를 보였다 (P<0.05). 전 처리구간에서 사료 요구율은 주령이 증가할수록 요구율이 증가하는 경향을 보였다. 5. 콜레스테롤은 대조구 + GTB 1.0%첨가구가 13.923으로 높게 나타났다. 가장 낮은 콜레스테롤은 남은 음식물 40%처리구가 12.003으로 낮게 나타났다. 남은 음식물을 첨가한 처리구가 난황 내 콜레스테롤 함량이 낮아지는 경향을 보였으나 남은 음식물 30%첨가구가 12.835로 높은 수치를 보였으며 통계적 유의차는 나타나지 않았다 (P>0.05). 6. TBA가 가장 높은 처리구간은 1주에는 남은 음식물 40%첨가구가 3.205로 높게 나타났으며 2주에는 남은 음식물30%첨가구가 3.275로 높게 나타났다. 마지막 주에는 3.947로 높게 나타났다. 이 결과로 볼 때 남은 음식물을 첨가한 처리구가 대체적으로 산패도가 높게 나타나는 경향을 보였으며 통계적 유의차는 나타나지 않았다 (P>0.05).

• Korean Journal of Environmental Agriculture
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• v.34 no.3
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• pp.186-191
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• 2015

BACKGROUND: Carotenoids which are a major source of vitamin A are contributed to have great potential role in anti-carcinogenic effects and eyesight. Carotenoids which can not synthesize in human body are required for food supply. The objectives of this study are to investigate compositions and contents of pumpkin (Cucurbita spp.) germplasms based on their pulp color. METHODS AND RESULTS: Carotenoids were extracted with 0.2% ascorbic acid in ethanol and saponified with 80% potassium hydroxide. Insoluble compounds were extracted into hexane. A total of nine carotenoids (three xanthophylls and six carotenes) were identified from pumpkin germplasms using HPLC equipped with photodiode array detector (450 nm). Especially, lutein and ${\beta}$-carotenes were major compound in germplasms. Among isomers of ${\beta}$-carotene, all-trans-${\beta}$-carotene (16-27% of total carotenoids) was predominant compositions. The mean of total carotenoid contents was showed as brown (286.1 mg/100 g dw) > dark green (217.0) > orange (153.4) > primrose (85.8) > dark yellow (80.3). On the basis of carotenoid information, PLS-DA score plots showed different patterns by cluster in pumpkin germplasms. It was considered that these differences of phenotype were relative closely to genotype. CONCLUSION: This study indicated that dark color of pumpkin pulp was presented in high-level of biological pigments. It may contribute to develop potentially beneficial functional food ingredients.