• 대한본초학회지
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• 제22권4호
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• pp.155-167
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• 2007

Objectives : This study was intended to compare genetic differences and anti-oxidative effects of Achyranthis Japonicae Radix, Achyranthis Bidentatae Radix and Cyathulae Radix. Methods : The genetic differences of Achyranthis Japonicae Radix, Achyranthis Bidentatae Radix and Cyathulae Radix were observed with RAPD analysis. Anti-oxidative effects of Japonicae Radix, Achyranthis Bidentatae Radix and Cyathulae Radix were investigated. Result : 1. As the result of RAPD analysis, Achyranthis Japonicae Radix was found to be genetically similar with Achyranthis Bidentatae Radix. 2. There was a little difference in the DPPH free radical scavenging activity, lipid peroxidation inhibiting activity, SOD activity of Achyranthis Japonicae Radix, Achyranthis Bidentatae Radix and Cyathulae Radix. Generally, Achyranthis Japonicae Radix was found to have stronger effect than the others. Conclusions : Achyranthis Japonicae Radix and Achyranthis Bidentatae Radix were genetically similar. Achyranthis Japonicae Radix, Achyranthis Bidentatae Radix and Cyathulae Radix all exhibited anti-oxidative action.

• 대한본초학회지
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• 제32권1호
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• pp.83-89
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• 2017

Objectives : The present study was designed to determine the effects of mixture of Achyranthis Japonicae Radix, Scutellariae Radix, and Acanthopanacis Cortex on monosodium iodoacetate (MIA)-induced osteoarthritis in rats. The mixture was composed of Achyranthis Japonicae Radix, Scutellariae Radix, and Acanthopanacis Cortex extracts. Methods : Arthritis was induced by injection of MIA into knee joints of rats. At the end of experiment, gross examination on the articular structures of knee joints were performed. Proteoglycan (PG) contents in articular cartilages were analysed as well. Tumor necrosis $factor-{\alpha}$($TNF-{\alpha}$) and $interleukin-1{\beta}$ ($IL-1{\beta}$) contents in synovial fluids were measured by ELISA method and matrix metalloproteinase 2 (MMP2), MMP9, and tissue inhibitors of metalloproteinase 1 (TIMP1) mRNA were measured by a realtime PCR. Results : The surfaces of the articular cartilage were observed. The severity of osteoarthritis in the treated group were alleviated compared with control group. PG contents in articular cartilages of the treated group were increased compared with control group. $IL-1{\beta}$ contents in synovial fluids of the treated group were significantly decreased compared with control group. MMP2 and MMP9 mRNA contents in articular cartilages were significantly decreased compared with control group and TIMP1 mRNA contents were increased compared with control group. Conclusions : On the basis of these results, we concluded that Achyranthis Japonicae Radix-containing mixture treatment has anti-arthritic effects on the MIA-induced osteoarthritis in rats. And the effects were related with the reduction of $IL-1{\beta}$ in synovial membranes and the consequent reduction of MMP2 and MMP9 expressions.