• Korean Journal of Medicinal Crop Science
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• v.7 no.1
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• pp.51-57
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• 1999

To search for antioxidative compounds from plant resources, methanol extracts of 45 plant species were investigated using DPPH method. The highest activity was shown in the methanol extract of Acer ginnala($RC_{50}\;:\;15{\mu}g$), followed by Stewartia koreana($RC_{50}\;:\;28{\mu}g$) and Carpinus laxiflora($RC_{50}\;:\;33{\mu}g$). Two antioxidative compounds were isolated from the methanolic extract of Acer ginnala Max and identified as acertannin(2, 6-di-O-galloyl-1, 5-anhydro-D-glucitol) and gallicin (methyl-3, 4, 5-trihydroxybenzoic acid) on the basis of mass spectroscopy, $^1H-\;and\;^{13}C-NMR$ data. The DPPH free radical scavenging activities of acertannin($RC_{50}\;:\;3.5{\mu}g$) and gallicin($RC_{50}\;:\;2.8{\mu}g$) were more effective than those of BHA($RC_{50}\;:\;14{\mu}g$) and ${\alpha}-tocopherol$ ($RC_{50}\;:\;12{\mu}g$).

• Journal of Korean Society of Forest Science
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• v.96 no.5
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• pp.551-560
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• 2007

We investigated physiological damages and biochemical alleviation of five species of genus Acer under ozone fumigation in order to assess their tolerant ability against ozone toxicity. At the end of 150 ppb $O_3$ fumigation, photosynthetic characteristics were measured, and chlorophyll contents, malondialdehyde (MDA) and antioxidative enzyme activities were analyzed in the leaves of five maple trees (Acer buergerianum, A. ginnala, A. mono, A. palmatum, and A. palmatum var. sanguineum). The reduction of chlorophyll (chl) a in ozone-exposed plants was 16.8% (A. buergerianum) to 26.7% (A. ginnala) of control plants. For the content of chi b, A. ginnala and A. palmatum var. sanguineum represented the high reduction of 26.3% and 23.6%, respectively. The highest reduction on the chi a:b ratio was observed in the leaves of A. palmatum. The reduction of net photosynthesis in five species varied from 2.4% to 37.6%. Among five species, A. ginnala showed remarkable reduction (37.6%) for net photosynthesis in comparison with control. Carboxylation efficiency differed significantly (P < 0.05) among species and between control and ozone treatment. The reduction of carboxylation efficiency was the highest in the leaves of A. ginnala (44.7%). A. palmatum var. sanguineum showed the highest increase (41.7%) for MDA content. The highest increase of superoxide dismutase (SOD) activity represented in A. palmatum (26.1%) and the increase of ascorbate peroxidase (APX) activity ranged from 16.5% (A. ginnala) to 49.1% (A. palmatum var. sanguineum). A. mono showed the highest increase (376.6%) of glutathione reductase (GR) activity under ozone fumigation and A. buergerianum also represented high increase (42.3%) of GR activity. Catalse (CAT) activity increased in the leaves of A. ginnala, A. palmatun and A. palmatum var. sanguineum under ozone exposure, whereas A. buergerianum and A. mono decreased in comparison with control plants. In conclusion, physiological markers such as chlorophyll content and photosynthesis that responded sensitively to $O_3$ in maple trees were considered as the very important indicators in order to evaluate the tolerance against $O_3$ stress, and parameters were closely related with each other. Among anti oxidative enzymes, SOD and APX might be contributed to alleviate to $O_3$ toxicity through the increase of activity in all maple trees. Therefore, these compounds can be used as a biochemical maker to assess the stress tolerance to $O_3$.

• Food Science and Preservation
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• v.11 no.4
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• pp.550-556
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• 2004

In the present study, we investigated the antimutagenic and cytotoxic effects of Acer ginnala Max. bark extract on S. typhimurium TA98, TA100 and cancer cell lines with Ames test and SRB assay, respectively. They were extracted with methanol and then fractionated using hexane, chloroform, ethyl acetate, butanol, and water to obtain the fractions. The inhibition rate of methanol ($200\;{\mu}g/plate$) of Acer ginnala Max. bark extract in the Salmonella typhimurium TA100 strain showed $83.3\%$ against the mutagenesis induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). In addition, the suppression of methanol extract with same concentration of in the Salmonella typhimurium TA98 and TA100 strains showed $80.3\%\;and\;92.7\%$ inhibition against 3-amino-1,4-dimethyl-5H-pyrido-(4,3-b)indol (Trp-P-1), respectively. The cytotoxicity effects of Acer ginnala Max. bark extract against the cell lines with human lung carcinoma (A549), human gastric carcinoma (AGS), human hepatocellular carcinoma (Hep3B) and human breast adenocarcinoma (MCF-7) were inhibited with the increase of the extract concentration. The treatment of 1.0 mg/mL Acer ginnala Max. bark methanol extract of methanol showed strong cytotoxicities of $77.3\%,\;90.4\%,\;88.9\%,\;and\;83.7\%$ against A549, AGS, Hep3B and MCF-7, respectively.

• Proceedings of the Zoological Society Korea Conference
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• 2003.08a
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• pp.117.2-117
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• 2003

• Journal of Korean Society for Atmospheric Environment
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• v.7 no.3
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• pp.208-218
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• 1991

To compare the resistance of landscape trees by acid rain, simulated acid rain was treated on the seeds of 6 coniferous species (Pinus densiflora, Pinus thunbergii, Pinus koraiensis, Taxus cuspidata, Cedrus deodara, Thuja occidentalis) and 6 broad-leafed species (Ligustrum obtusifolium, Acer Buergerianum, Acer Ginnala, Styrax obassia, Cornus Kousa, Magnolia obovata). The experimental design of randomized block arrangement with three replications was implemented in the experimental field of Yesan National Agri-Tech Junior College. The seeds of 12 tree species were planted on the nursery soil in the early spring of 1991. The regime of artificial acid rain, in terms of spray frequency per monthly and spray amount at year records. Simulated acid rain (pH 2.0, pH 4.0, pH 5.6 as control) containing sulfuric and nitric acid in the ratio of 3:2 (chemical equivalant basis) diluted ground water, were treated on the experimental plants under condition of cutting off the natural precipitation with vinyl tunnel, during the growing season (April 8 to August 31) in 1991. Seedling establishment, seedling growth and nutrient contents in needles and leaves were measured and compared among the treatment. The results were summerized as follows; 1. In general, coniferous species were more resistant than broad-leafed species in the resistant comparisons of landscape trees by simulated acid rain. But there were resistant tree species to acid rain among the broad-leafed species. 2. Among 6 coniferous species used in this experiment, Pinus densiflora, Pinus koraiensis, Taxus cuspidata, Cedrus deodara and Pinus thunbergii revealed the resistant tree species to acid rain. But Thuja occidentalis was very susceptible to acid rain, because of no germination in pH 2.0 treatment plot of acid rain. 3. Among 6 broad-leafed species used in this study, Magnolia obovata, Styrax obassia and Ligustrum obtusifolium showed relative resistant tree species to acid rain. But Acer Buergerianum, Acer Ginnala and Cornus Kousa were very susceptible to acid rain, because of no germination in pH 2.0 plot of acid rain treatment.

• Journal of Korean Society of Forest Science
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• v.67 no.1
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• pp.1-9
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• 1984

Taxonomical studies of the genus Acer in Korea (8 species, 3 varieties and 1 forma) were studied by the morphological characteristics of stipules. The results obtained were summarized as follows; 1) The stipules of the genus Acer were arranged oppositely with 4 - 10 stipules consisting of simple leaf, compound leaf and unifoliate compound leaf. The venation of stipules were cladodromous, basal reticulate, fibrous and palmately netted vein. The trichoma of stipules were glandula hair, glandular pubescent, sericeous, pilous, vinous, velutinous, tomentose, ciliate and lanate. As the morphological characteristics of stipules in the genus Acer established a strong point in the identification of species. 2) The species in the genus Acer in Korea were classified into six groups; (1) ginnala group (A. ginnala), (2) koreanum group (A. koreanum, A. japonicum, A. pseudosieboldianum var. koreanum), (3) mono group (A. mono, A, palmatum var. sanguineum, A. palamtum var. dissectum, A. palmatum var. dissectum f. aosidare), (4) negundo group (A. negundo), (5) palmatum group (A. palmatum), (6) platanoides group (A. platanoides, A. truncatum) by the morphological characteristics of stipules, 3) The stipules of platanoides group and negundo group were the least evolved types among the species in the genus Acer. 4) The stipules in the genus Acer were made the inner buds that were metamorphosed to the protected functional aspect by the evolution of petiols.

• Journal of the Korean Institute of Landscape Architecture
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• v.18 no.2
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• pp.57-65
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• 1990

To select suitable siecies for barrier trees in the middle tmperature forest zone of Korea, sprout ratio, verdi cal density ratio and impenetrability were analyzed for 41 species including 9 evergreen conifer trees which had been transplanted 18 years ago and maintained as a hedge at Pocheon - Gun, Kyonggi -Do, Korea. 1. The species with high sprout ratio of hedges were Rosa multiflora, Lonicera maackii, Chamaecyparis pisifera, Thuja occidentalis in order at the upper portion, Thuja occidentalis, Rosa multiflora, Chamaecyparis pisifera, in order oa the middle portion, and Thuja occidentals, Chamaecyparis pisigera, Rosa multiflora, Cercdiphyllum japonicum, Cornus kousa, Ulmus pumila, Robinia pseudoacacia in order at the lower portion of hedges. 2. the species with high vertical density ratio were Thuja occidentalis, Chamaecyparis pisifera, Weigela subsessilis, Acer ginnala in order. 3.The species with hight impnetrability were Thuja occidentalis, Chamaecyparis pisifera, Forsythia Koreana, Juniperus rigida, Juniperus chinensis, Pinus strobus in order. 4. the values of correlation coefficents between the sprout ratio and impenetrability are given in table 3 and positive significant correlations can be observed in conifer trees.5. The species with high suitability for tree barrier were Thuja occidontalis, Chamaecyparis pisifera, Acer ginnala, Juniperus chinensis, Rosa multiflora, Counus kousa, Cercidiphyllum japonocum, Weigela subsessilis, Cornus officinalis, Philadelphus schrenckii in order.

• Korean Journal of Environment and Ecology
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• v.21 no.5
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• pp.390-399
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• 2007

We analyzed the vegetation environment at the stream section of habitat in which Cottus koreanus lives. The plant communities were classified into Fraxinus rhynchophylla community, Acer ginnala-Salix koreensis community, Phragmites japonica-Salix gracilistyla community, Salix gracilistyla community, Phragmites japonica community, Robinia pseudo-acacia community, and Oenanthe javanica-Persicaria thunbergii community. The frequency of Phragmites japonica-Salix gracilistyla community was the most. And also the frequencies of Fraxinus rhynchophylla community and Salix gracilistyla community were high. In stream bank zone and terrace zone, the typically represented vegetation was the Fraxinus rhynchophylla community and Acer ginnala-Salix koreensis community and Phragmites japonica-Salix gracilistyla community, respectively. The rNCD of the top-priority plants applied to restoration of the vegetation in stream bank zone came out by the order of Ulmus davidiana var. japonica, Salix koreensis, Acer ginnala, Salix gracilistyla, and Fraxinus rhynchophylla. The rNCD in terrace zone was arranged by the order of Salix gracilistyla, Phragmites japonica, and Artemisia princeps. The spatial distribution of vegetation was the most greatly influenced by cross-sectional position of the surveyed stream, and this aspect is believed to be due to the habitat's physical stability. Species diversity also obviously makes a great difference depending on their surrounding physical stability.

• Journal of the Korean Wood Science and Technology
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• v.31 no.6
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• pp.40-44
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• 2003

This study was carried out to investigate the antioxidant activities of domestic trees grown in Korea. Based on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity method, the methanolic extracts of 23 species were screened in order to search for natural antioxidants. Among these species, Acer ginnala, Cotinus coggygria, Acanthopanax koreanum, Thea sinensis and Pinus densiflora showed stronger antioxidative activity comparing with reference compound, ascorbic acid.

• The Korean Journal of Ecology
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• v.18 no.1
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• pp.137-146
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• 1995

Acer pseudo-sieboldianum, A. ginnala, A. negundo and A. saccharinum were selected as materials for the studies on the seasonal fluctuation of chlorophyll content and photosynthetic rates. In all the four species during the growing season except in October, the principal component that determined the total chlorophyll content was chlorophyll a. Content of chlorophyll b increased with leaf age, but that of chlorophyll a decreased. In contrast to A. saccharinum and A. pseudo-sieboldianum which showed their maximum chlorophyll content in June, A. negundo, which showed the highest chlorophyll content of the four species, exhibited its maximum chlorophyll content in July, whereas September in the case of A. ginnala. The fluctuation of chlorophyll content was similar to that of air temperature, and it increased till July. But chloprophyll content showed a significant relationship in early stage of leaf development, and there could be any interdependence between them in accordance with the seasonal change, no longer. In all four species light compensation points decreased in accordance with the seasonal change, and the maximum photosynthetic rates were obtained in August. Respiratory rates were shown no significant difference among species, and they decreased according to the adbance of season.