• Biomedical Science Letters
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• v.25 no.4
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• pp.426-430
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• 2019

Currently, molecular diagnostic assays based on nucleic acid amplification tests have been shown to effectively detect mycobacterial infections in various types of specimen, however, variable sensitivity was shown in FFPE samples according to the kind of commercial kit used. The present study therefore used automated PCR-reverse blot hybridization assay (REBA) system, REBA Myco-ID HybREAD 480^®, for the rapid identification of Mycobacterium species in various types of human tissue and compared the conventional one-tube nested-PCR assay for detecting Mycobacterium tuberculosis (MTB). In conventional nested-PCR tests, 25 samples (48%) were MTB positive and 27 samples (52%) were negative. In contrast, when conducted PCR-REBA assay, 11 samples (21%) were MTB positive, 20 samples (39%) were NTM positive, 8 samples (15%) were MTB-NTM double positive, and 13 samples (25%) were negative. To determine the accuracy and reliability of the two molecular diagnostic tests, the one-tube nested-PCR and PCR-REBA assays, were compared with histopathological diagnosis in discordant samples. When conducted nested-PCR assay, 10 samples (59%) were MTB positive and seven samples (41%) were negative. In contrast, when conducted PCR-REBA test, three samples (17%) were MTB positive, 10 samples (59%) were NTM positive and four samples (24%) were negative. In conclusion, the automated PCR-REBA system proved useful to identify Mycobacterium species more rapidly and with higher sensitivity and specificity than the conventional molecular assay, one-tube nested-PCR; it might therefore be the most suitable tool for identifying Mycobacterium species in various types of human tissue for precise and accurate diagnosis of mycobacterial infection.

• Composites Research
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• v.29 no.1
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• pp.24-32
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• 2016

Ballistic impact analyses have been performed with the Kevlar fabric impregnated with STF(shear thickening fluid). Multi-layer laminates modeled with 3D isoparametric shell elements were used for the performance analysis and their results are compared with experimental results. Both experiments and numerical analyses have been done to verify the usefulness of STF to enhance the impact resistance performance. The results showed that STF increases friction within a bundle of fiber, and this phenomena is more apparent in the velocity range of under near 450 m/s. In this research, it is emphasized that FEA analyses of STF impregnated Kevlar fabric laminate were successfully conducted using shell elements. Moreover, the effectiveness of the technique and accuracy were verified through the comparison with reliable experimental data.

• Journal of the Korean Society of Marine Environment & Safety
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• v.21 no.5
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• pp.599-607
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• 2015

The purpose of this study is to reproduce long-term wave fields in coastal waters of Korea based on wave hind-casting modelling and discuss its applications. To validate wind data(NCEP, ECMWF, JMA-MSM), comparison of wind data was done with wave buoy data. JMA-MSM predicted wind data with high accuracy. But due to relatively longer period of ECMWF wind data as compared to that of JMA-MSM, wind data set of ECMWF(2001~2014) was used to perform wave hind-casting modelling. Results from numerical modelling were verified with the observed data of wave buoys installed by Korea Meteorological Administration(KMA) and Korea Hydrographic and Oceanographic Agency(KHOA) on offshore waters. The results agree well with observations at buoy stations, especially during the event periods such as a typhoon. Consequently, the wave data reproduced by wave hind-casting modelling was used to obtain missing data in wave observation buoys. The obtained missing data indicated underestimation of maximum wave height during the event period at some points of buoys. Reasons for such underestimation may be due to larger time interval and resolution of the input wind data, water depth and grid size etc. The methodology used in present study can be used to analyze coastal erosion data in conjunction with a wave characteristic of the event period in coastal areas. Additionally, the method can be used in the coastal disaster vulnerability assessment to generate wave points of interest.

• Journal of Yeungnam Medical Science
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• v.8 no.1
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• pp.42-52
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• 1991

Two types of urine dipstick assays, Multistix-SG and Combur-9-test RL, were compared for compatibility, accuracy, specificity and predictive values of a positive and negative test in 501 patients urine and artificially prepared specimen. We found that the results of semiquantitative tests of Multistix-SG and Combur-9-Test RL performed were statistically similar in patients specimen. The urinary leukocyte estrase tests of Combur-9-Test RL assays compared with urine sediment microscopy in regard to compatibility, sensitivity, specificity, and predictive values of a positive and negative test 83.7%, 48.1%, 90.3%, 47.4% and 90.1%, respectively. The urinar nitrite tests of Comber-9-Test RL assays compared with urine culture tests, in regard to compatibility, sensitivity, specificity, and predictive values of a positive and negative tests were 90.3%, 19.4%, 84.7%, 53.8% and 94.1%, respectively. For the urinary protein, the sulfosalicylic acid method was the most sensitive test for any kinds of protein, and Multistix-SG appeared more sensitive than Compur-9-Test RL for the albuminuria. For the urinary bilirubin and glusose, two dipstick assays were similar in their diganostic efficiency. Finally in the urinary occult blood tests, Combur-9-Test RL assays was more sensitive than Multistix-SG.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.29 no.1
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• pp.5-13
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• 2003

The purpose of this study is to determine the value of serum concentration of C-reactive protein(CRP) in comparison with ESR and leukocyte as the tools for diagnosis and follow-up study of infection. For this study, thirty-one patients with head and neck infection and thirty-two patients performed the orthognathic surgery were selected for experimental and control groups each other. we analyzed the blood sample daily to measure CRP, ESR and leukocytosis. The results of this study are as the following : 1. Serum C-Reactive Protein levels in the experimental group were higher than control group and the pattern of CRP changes continued to decline in both groups(P<0.001). 2. ESR changes in both groups were scattered without any special pattern. 3. Correlation between CRP and Leukocyte was higher than others(r=0.664, P<0.01). 4. In the Receiver Operating Characteristic(ROC) Curve of experimental group, rapid increase of CRP curve and Area under the curve(AUC) value, 0.774, indicate the high accuracy of estimation. 5. In experimental group, sensitivity of CRP, ESR, and Leukocytosis on recovery day were 83%, 17% and 71%. Based on the results of this study, we could conclude that determination of CRP is more useful method to diagnosis and follow-up study of infection than other commonly used variables in oral and maxillofacial region.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.12 no.4
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• pp.525-535
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• 1999

In this study, a new method of fabricated concrete deck bridge construction is proposed. This paper details the method in which concrete multi-girders and fabricated concrete decks are rested on the upper flange of the girder and the female to female type sheat-key is formed to connect girder and deck. The finite element analysis is performed to verify the accuracy of the structural behaviors of the fabricated concrete deck bridge by comparing with experimental results. The first task performed is the analysis of the equilibrium of the member force occurring between the deck and the girder. After verifying equilibrium of the member force determined by the finite element analysis, this process is applied to the analysis of maximum member force as the position of design load. This task is utilized to determine the safety of each member according to the same scale finite element model. The final process in this study is to compare the deflection of girders used in experiment with that of the same scale finite element model to verify the strength of fabricated cincrete deck bridge. By this comparison, it is shown that the behavior of the fabricated concrete deck bridge is almost same as the finite element analysis. The second task is to analyze the load distribution effect according to the number of diaphragms and the composite effect due to the cinnection of the deck and girder by the finite element analysis. From the results of second task, it is found that the load distribution effect is not related to the number of diaphragms in case of the central loading, but is related to the number of diaphragms for eccentric loading. Analysis of the load distribution indicates that the effective number of diaphragm is three. It is also shown that the maximum deflection is decreased to almost one half due to the composite action of the deck and girder.

• Journal of the Korea Society for Simulation
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• v.16 no.1
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• pp.11-19
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• 2007

Under ubiquitous environment, applications can gather and utilize various sensing information. There are many issues such as energy management, protocol standardization, independency on sensor fields, and security to be resolved for the complete ubiquitous computing. Especially, the independent information access in the sensor field is one of the most important issues to maximize the usability of sensors in various sensor fields. However, existing frameworks are not suitable for the ubiquitous computing environment because of data heterogeneity between data elements in sensor fields. Existing applications are dependent to sensor fields and sensors in the existing ubiquitous computing on environment is dependent to the application in the sensor field. In other word, an application can utilize just information from a specific sensor field. To overcome this restriction, many issues from a hardware or software view must be resolved. In this paper, we provide the design and implementation of the Metadata Registry-based framework (UbiMDR) of the Ubiquitous environment. This framework can provides the semantic interoperability among ubiquitous applications or various sensor fields. In addition, we describe comparison evaluation between conventional Ubiquitous computing framework and UbiMDR framework with data accuracy of interoperability.

• Journal of Korea Water Resources Association
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• v.43 no.4
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• pp.383-391
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• 2010

The groundwater recharge was assessed by using both SWAT and HELP models in Bocheong-cheon watershed. The SWAT model is a comprehensive surface and subsurface model, but it lacks the physical basis for simulating a soil water percolation process. The HELP model which has a drawback in simulating subsurface lateral flow and groundwater flow component can simulate soil water percolation process by considering the unsaturated flow effect of soil layers. The SWAT model has been successfully applied for estimating groundwater recharge in a number of watersheds in Korea, while the application of HELP model has been very limited. The subsurface lateral flow parameter was proposed in order to consider the subsurface lateral flow effect in HELP model and the groundwater recharge was simulated by the modified exponential decay weighting function in HELP model. The simulation results indicate that the recharge of HELP model significantly depends on the values of lateral flow parameter. The recharge errors between SWAT and HELP are the smallest when the lateral flow parameter is about 0.6 and the recharge rates between two models are shown to be reasonably comparable for daily, monthly, and yearly time scales. The HELP model is useful for estimating groundwater recharge at watershed scale because the model structure and input parameters of HELP model are simpler than that of SWAT model. The accuracy of assessing the groundwater recharge might be improved by the concurrent application of SWAT model and HELP model.

• Journal of Korea Water Resources Association
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• v.41 no.5
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• pp.491-501
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• 2008

In order to describe runoff characteristics of urban drainage area, outflow from subbasins divided by considering topography and flow path, is analyzed through stormwater system. In doing so, concentration time and time-area curve change significantly according to basin shape, and runoff characteristics are changed greatly by these attributes. Therefore, in this development study of FFC2Q model by MLTM, we aim to improve the accuracy in analyzing runoff by adding a module that considers basin shape, giving it an advantage over popular urban hydrology models, such as SWMM and ILLUDAS, that can not account for geometric shape of a basin due to their assumptions of unit subbasin as having a simple rectangular form. For subbasin shapes, symmetry types (rectangular, ellipse, lozenge), divergent types (triangle, trapezoid), and convergent types (inverted triangle, inverted trapezoid) have been analyzed in application of time-area curve for surface runoff analysis. As a result, we found that runoff characteristic can be quite different depending on basin shape. For example, when Gunja basin was represented by lozenge shape, the best results for peak flow discharge and overall shape of runoff hydrograph were achieved in comparison to observed data. Additionally, in case of considering subbasin shape, the number of division of drainage basin did not affect peak flow magnitude and gave stable results close to observed data. However, in case of representing the shape of subbasins by traditional rectangular approximation, the division number had sensitive effects on the analysis results.

• Journal of Korea Water Resources Association
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• v.47 no.11
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• pp.1039-1050
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• 2014

The goal of this study is to demonstrate the diversity of model performance for various climatic elements and indicators. We evaluated the skills of the most advanced 17 General Circulation Models (GCMs) i.e., CMIP5 (Climate Model Inter-comparison project, phase 5) climate models in reproducing retrospective climatology from 1950 to 2000 over the Southeast US for the key climatic elements important in the hydrological and agricultural perspectives (i.e., precipitation, maximum and minimum temperature, and wind speed). The biases of raw CMIP5 GCMs were estimated for 16 different climatic indicators that imply mean climatology, temporal variability, extreme frequency, etc. using a grid-based observational dataset as reference. Based on the error (RMSE) and correlation (R) of GCM outputs, the error-based GCM ranks were assigned on average over the indicators. Overall, the GCMs showed much better accuracy in representing mean climatology of temperature comparing to other elements whereas few GCM showed acceptable skills for precipitation. It was also found that the model skills and ranks would be substantially different by the climatic elements, error statistics applied for evaluation, and indicators as well. This study presents significance of GCM uncertainty and the needs of considering rational strategies for climate model evaluation and selection.