• Title/Summary/Keyword: Accumulation encoding

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Accumulation Encoding Technique Based on Double Random Phase Encryption for Transmission of Multiple Images

  • Lee, In-Ho
    • Journal of the Optical Society of Korea
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    • v.18 no.4
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    • pp.401-405
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    • 2014
  • In this paper, we propose an accumulation encoding scheme based on double random phase encryption (DRPE) for multiple-image transmission. The proposed scheme can be used for a low-complexity DRPE system due to the simple structure of the accumulation encoder and decoder. For accumulation encoding of multiple images, all of the previously encrypted data are added, and hence the accumulation encoding can improve the security of the DRPE-encrypted data. We present a scheme for encryption and decryption for DRPE-based accumulation encoding, and a method for accumulation encoding and decoding. Finally, simulation results verify that the DRPE-based accumulation encoding scheme for multiple images is powerful in terms of data security.

Effect of introduction of fumarase on the production of succinic acid

  • Hong, Sun-Ho;Lee, Sang-Yeop
    • 한국생물공학회:학술대회논문집
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    • 2000.04a
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    • pp.531-534
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    • 2000
  • The fumB gene encoding anaerobic fumarase of Escherichia coli XL1-Blue was introduced to solve the malic acid accumulation problem. When NZN111 harboring pTrcMLFu was cultured, 7 g/L of succinic acid was produced and malic acid was not accumulated.

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Characterization and refinement of enzyme of the gene encoding catechol 1,2-dioxygenase from Phenol-degrading, Rhodococcus sp.

  • 이희정;박근태;박재림;이상준
    • Proceedings of the Korean Environmental Sciences Society Conference
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    • 2002.05b
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    • pp.209-212
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    • 2002
  • The heavy use of petroleum products in modern livings has brought ubiquitous environmental contaminants of aromatic compounds, which persist in aquatic and geo-environment without the substantial degradation. The persistence and accumulation of the aromatic compounds, which include xylene, phenol, toluene, phthalate, and so on are known to cause serious problems in our environments. Some of soil and aquatic microorganisms facilitate their growth by degrading aromatic compounds and utilizing degrading products as growth substrates, the biodegradation helps the reentry of carbons of aromatic compounds, preventing their accumulation in our environments. The metabolic studies on the degradation of aromatic compounds by microoganlsms were extensively carried out along with their genetic studies. A Rhodococcus sp. isolated in activated sludges has shown the excellent ability to grow on phenol as a sole carbon source. In the present study investigated a gene encoding phenol-degrading enzymes from a Rhodococcus sp.

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Isolation and Characterization of a Cdna ( Fp 1 ) Encoding the Iron Storage Protein in Red Pepper ( Capsicum annuum L. )

  • Kim, Ho-Young;Lee, Young-Ok;Noh, Ill-Sup;Kang, Hee-Wan;Kameya, Toshiaki;Saito, Takashi;Kang, Kwon-Kyoo
    • Plant Resources
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    • v.1 no.1
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    • pp.13-21
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    • 1998
  • A cDNA Fragment encoding iron storage protrin generated by polymerase chain reaction(PCR) using highly conserved regions of ferritin related genes were used to sereen a red pepper cDNA library. cDNA clone was designated as Fp1. Fp1 clone contatines a 5' nontranslated region of 51dp containing stop conds. Down stream from 5' UTP. an open reading frame of 750bp was observed. followed by a 3' UTR of 272bp. The deduces amino acid sequence of red pepper protein(Fp1) showed 84%, 48% and 36% identity with soybean(SolC). human(HuL H) and horse spleen(HoS-L) ferritin mRNA accumulation in response to iron. Ferritin mRNA accumulation was transient and particularly abundant in leaves. reaching a maxmum at 12h. The level of ferritin mRNA in roots was affected to a lesser extent than in leaves.

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Development of a Genome-Wide Random Mutagenesis System Using Proofreading-Deficient DNA Polymerase ${\delta}$ in the Methylotrophic Yeast Hansenula polymorpha

  • Kim, Oh Cheol;Kim, Sang-Yoon;Hwang, Dong Hyeon;Oh, Doo-Byoung;Kang, Hyun Ah;Kwon, Ohsuk
    • Journal of Microbiology and Biotechnology
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    • v.23 no.3
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    • pp.304-312
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    • 2013
  • The thermotolerant methylotrophic yeast Hansenula polymorpha is attracting interest as a potential strain for the production of recombinant proteins and biofuels. However, only limited numbers of genome engineering tools are currently available for H. polymorpha. In the present study, we identified the HpPOL3 gene encoding the catalytic subunit of DNA polymerase ${\delta}$ of H. polymorpha and mutated the sequence encoding conserved amino acid residues that are important for its proofreading 3'${\rightarrow}$5' exonuclease activity. The resulting $HpPOL3^*$ gene encoding the error-prone proofreading-deficient DNA polymerase ${\delta}$ was cloned under a methanol oxidase promoter to construct the mutator plasmid pHIF8, which also contains additional elements for site-specific chromosomal integration, selection, and excision. In a H. polymorpha mutator strain chromosomally integrated with pHIF8, a $URA3^-$ mutant resistant to 5-fluoroorotic acid was generated at a 50-fold higher frequency than in the wild-type strain, due to the dominant negative expression of $HpPOL3^*$. Moreover, after obtaining the desired mutant, the mutator allele was readily removed from the chromosome by homologous recombination to avoid the uncontrolled accumulation of additional mutations. Our mutator system, which depends on the accumulation of random mutations that are incorporated during DNA replication, will be useful to generate strains with mutant phenotypes, especially those related to unknown or multiple genes on the chromosome.

Real-Time PCR Analysis of Metabolic Pathway of PHB in Acidiphilium cryptum DX1-1

  • Xu, Ai-Ling;Xia, Jin-Lan;Liu, Ke-Ke;Li, Li;Yang, Yu;Nie, Zhen-Yuan;Qiu, Guan-Zhou
    • Journal of Microbiology and Biotechnology
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    • v.20 no.1
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    • pp.71-77
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    • 2010
  • The time, yield, and related genes expression of PHB accumulation of Acidiphilium cryptum DX1-1 were investigated under four different initial C/N ratios, 1.2, 2.4, 7.5, and 24. The results of time and yield of poly-$\beta$-hydroxybutyrate (PHB) accumulation show that the initial C/N ratio of 2.4 was optimum for strain DX1-1 to accumulate PHB, but both higher and lower initial C/N ratios did not favor that process. Based on the genome of Acidiphilium cryptum JF-5, 13 PHB accumulation related genes in strain JF-5 were chosen and successfully cloned from strain DX1-1. The differential expressions of the 13 functional genes, in different C/N ratios as cited above, were then studied by real-time PCR. The results show that all the 13 genes were most upregulated when the initial C/N ratio was 2.4, and among which the gene Acry_3030 encoding poly-$\beta$-hydroxybutyrate polymerase and Aery_0626 encoding acetyl-CoA synthetase were much more upregulated than the other genes, which proved that they play the most important role for PHB accumulation, and acetate is the main initial substance for PHB accumulation for strain DX1-1. Potential regulatory motifs analysis showed that the genes related to PHB accumulation are regulated by different promoters and that the motif had weak similarity to the model promoters, suggesting that PHB metabolism in Acidiphilium cryptum may be mediated by a different mechanism.

Proline accumulation and transcriptional regulation of proline biothesynthesis and degradation in Brassica napus

  • Xue, Xingning;Liu, Aihua;Hua, Xuejun
    • BMB Reports
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    • v.42 no.1
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    • pp.28-34
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    • 2009
  • To understand the molecular mechanism underlying proline accumulation in Brassica napus, cDNAs encoding ${\Delta}^1$-pyrroline-5-carboxylate synthetase (BnP5CS), ornithine $\delta$-aminotransferase (BnOAT) and proline dehydrogenase (BnPDH) were isolated and characterized. Southern blot analysis of BnP5CSs in B. napus and its diploid ancestors suggested a gene loss may have occurred during evolution. The expression of BnP5CS1 and BnP5CS2 was induced, while the expression of BnPDH was inhibited under salt stress, ABA treatment and dehydration, prior to proline accumulation. The upregulation of BnOAT expression was only detected during prolonged severe osmotic stress. Our results indicate that stress-induced proline accumulation in B. napus results from the reciprocal action of activated biosynthesis and inhibited proline degradation. Whether the ornithine pathway is activated depends on the severity of stress. During development, proline content was high in reproductive organs and was accompanied by markedly high expression of BnP5CS and BnPDH, suggesting possible roles of proline during flower development.

7.7 Gbps Encoder Design for IEEE 802.11ac QC-LDPC Codes

  • Jung, Yong-Min;Chung, Chul-Ho;Jung, Yun-Ho;Kim, Jae-Seok
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.14 no.4
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    • pp.419-426
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    • 2014
  • This paper proposes a high-throughput encoding process and encoder architecture for quasi-cyclic low-density parity-check codes in IEEE 802.11ac standard. In order to achieve the high throughput with low complexity, a partially parallel processing based encoding process and encoder architecture are proposed. Forward and backward accumulations are performed in one clock cycle to increase the encoding throughput. A low complexity cyclic shifter is also proposed to minimize the hardware overhead of combinational logic in the encoder architecture. In IEEE 802.11ac systems, the proposed encoder is rate compatible to support various code rates and codeword block lengths. The proposed encoder is implemented with 130-nm CMOS technology. For (1944, 1620) irregular code, 7.7 Gbps throughput is achieved at 100 MHz clock frequency. The gate count of the proposed encoder core is about 96 K.

A Study On the Application Methods of a Support Vector Machine for Gene Promoter Prediction. (유전자 프로모터 예측을 위한 Support Vector Machine의 응용 방법에 대한 연구)

  • Kim, Ki-Bong
    • Journal of Life Science
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    • v.17 no.5 s.85
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    • pp.714-718
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    • 2007
  • The high-throughput sequencing of a lot of genomes has resulted in the relatively rapid accumulation of an enormous amount of genomic sequence data. In this context, the problem posed by the detection of promoters in genomic DNA sequences via computational methods has attracted considerable attention in recent years since exact promoter prediction can give a clue to the elucidation of overall genetic networks. In this study, applications of support vector machine(SVM) to promoter prediction are explored to show a right approaches to discriminate between promoter and non-promoter regions by means of SVM. The results of various experiments show that encoding method, encoding region and learning data constitution can play an important role in the performance of SVM.