• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.48 no.6
• /
• pp.447-451
• /
• 2003

When sowing green peas in the autumn, proper seedling stands and growth quantity should be secured before winter begins. Also, for proper acclimatization, injuries caused by low temperatures, frost or high temperatures in the P.E. film during mulching, should be avoided during the regeneration period; that being early spring. The days required for growth in each stage in Yeosu are shorter than those in Naju because Yeosu has high temperatures during the growth period. Furthermore, in Yoesu, it was observed that there were more effective branches as well as effective and attached node positions on the branches. The first pods on the main stems and effective branches were observed to be higher than those in Naju. The number of pods per plant and the number of seeds per pod in Yoesu was greater than for those in Naju and the pod length was longer as well. Considering the missing plant rate, growth, and green pod yield, the optimum sowing date for the green pea was mid-November in both location. The stable acclimatizing date for the green pea was early March when the highest yield can be acquired due to a lot of effective branches and pods per plant and with the lowest missing plant rate and rate of injury in acclimatization.

• Korean Journal of Medicinal Crop Science
• /
• v.13 no.4
• /
• pp.133-137
• /
• 2005

Experiments were conducted to find the optimal acclimatization conditions for Eleutherococcus senticosus plantlets regenerated from bioreactor cultured somatic embryos, various acclimatizing conditions were compared regarding both survival rate and growth of the plantlets. Among the various temperature and artificial soil tested, the highest survival rate (88%) was observed when plantlets were acclimatized in Klasmann bed soil at $10^{\circ}C$ When in vitro plantlets directly transplanted to field environment, shading treatment was necessary and 50% shading was more effective than 30% shading. Transplanting season were also important for successful acclimatization of in vitro cultured plantlets, transplanted on March 20 with 50% shading exhibited the best survival rate and further growth.

• Journal of Korean Society of Forest Science
• /
• v.97 no.1
• /
• pp.127-133
• /
• 2008

This study was conducted to establish the optimal condition for plant regeneration and acclimatization from somatic embryos of Panax ginseng. Cotyledon segments of Panax ginseng produced primary and secondary somatic embryos when cultured on MS and WPM media supplemented with 7% sucrose. To induce plantlet conversion, cotyledonary somatic embryos were cultured on WPM solid medium with $GA_3$ at various concentrations (1~30 mg/L) for 4 weeks. Plantlets were transferred to 1/2 WPM solid medium with $GA_3$ at various concentrations (0~5 mg/L) and 0.5% activated charcoal for shoot and root elongations. Elongated plantlets further developed into well-developed leaf and root system on 1/3 SH medium with 0.5% activated charcoal under ventilation condition for 5 months. The highest survival rate to soil was 75% when plantlets were regenerated on 1/3 SH medium without sucrose under ventilation condition.

• Journal of the Korean Society of Clothing and Textiles
• /
• v.21 no.4
• /
• pp.669-676
• /
• 1997

The purpose of this study is to examine the effect of the improved cold tolerance resulting from cool clothing in winter on heat tolerance in summer. Ten healthy women were divided into two groups, cold group(C group) (n=5) and warm group(W group) (n=5) . In the previous study, C group was proved that their cold acclimatization was achieved through wearing cool clothing from September to February of the following year, while Wgroup was not proved because of wearing warm clothing during same period. After February, no more clothing training was continued in two groups. To determine the heat tolerance, both groups were exposed from a thermoneutral environment(25$\pm$1$^{\circ}C$, 50$\pm$5% R.H.) to a hot environment (35$\pm$1$^{\circ}C$, 50$\pm$5% R.H.) before and after clothing training, respectively September in 1994 and truly in 1995. Rectal temperature, skin temperatures, thermal sensation and comfort were measured every 10 min., and Os uptake was measured at 10, 45, 85 min. after entering the chamber for 5 min. Body weight was measured before and after the experiment and amount of local sweat was measured during the 90 min long experiment. The results are as follows: Rectal temperatures in 35'c environment of C group were increased after training when compared with before clothing, while those of W group were not changed. But the changes of rectal temperature and heat production during 90 min in hot environment were almost the same in two groups after training. And mean skin temperatures, the changes of mean skin temperatures during 90 min in hot environment, total sweat amount and local sweat amount after training were also the same in two groups. From these results, it might be supposed that the heat loss of two groups were the same but the heat production, especially heat production during rest in C group was higher than in Wgroup. This fact suggests that the increase of rest heat production from cold acclimatization in winter is maintained to summer of the following year. And mild cold acclimatization coming from westing cool clothing does not have a negative effect on heat tolerance.

• The Korean Journal of Physiology and Pharmacology
• /
• v.19 no.1
• /
• pp.9-14
• /
• 2015

We investigated the sweating response during passive heating (partial submersion up to the umbilical line in $42{\pm}0.5^{\circ}C$ water, 30 min) after summer and winter seasonal acclimatization (SA). Testing was performed in July during the summer, 2011 [summer-SA; temp, $25.6{\pm}1.8^{\circ}C;$ relative humidity (RH), $82.1{\pm}8.2%$] and in January during the winter, 2012 (winter-SA; temp, $-2.7{\pm}2.9^{\circ};$ RH, $65.0{\pm}13.1%$) in Cheonan ($126^{\circ}52^{\prime}N$, 33.38'E), Republic of Korea. All experiments were carried out in an automated climatic chamber (temp, $25.0{\pm}0.5^{\circ}C$: RH, $60.0{\pm}3.0%$). Fifteen healthy men (age, $23.4{\pm}2.5$ years; height, $175.0{\pm}5.9cm;$ weight, $65.3{\pm}6.1kg$) participated in the study. Local sweat onset time was delayed during winter-SA compared to that after summer-SA (p<0.001). Local sweat volume, whole body sweat volume, and evaporative loss volume decreased significantly after winter-SA compared to those after summer-SA (p<0.001). Changes in basal metabolic rate increased significantly after winter-SA (p<0.001), and tympanic temperature and mean body temperature were significantly lower after summer-SA (p<0.05). In conclusion, central sudomotor acitivity becomes sensitive to summer-SA and blunt to winter-SA in Rebubic of Korea. These results suggest that the body adjusts its temperature by economically controlling the sweating rate but does not lower the thermal dissipation rate through a more effective evaporation scheme after summer-SA than that after winter-SA.

• Journal of Plant Biotechnology
• /
• v.49 no.2
• /
• pp.124-130
• /
• 2022

The application of traditional taro propagation methods for large-scale cultivation would be insufficient to meet the high demand for quality planting materials. Therefore, this study aimed to develop an in vitro micro-propagation technique for two local taro cultivars (cv.), Wangi and Putih. Taro cormels were collected from the Malaysian Agricultural Research and Development Institute (MARDI) germplasm (Serdang, Malaysia). Explants were taken from the shoot tip of cormels and initially cultured on Murashige and Skoog (MS) basal media for four weeks. The explants were then transferred to different multiplication media supplemented with different types and concentrations of cytokinins such as 6-benzylaminopurine (BAP ) and Thidiazuron (TDZ). Shoot production was quantified after six weeks of culture. The highest mean number of new shoots was produced by the Wangi cultivar on MS medium supplemented with 2.0 mg/l BAP (2.10 shoots), MS medium supplemented with 0.5 mg/l TDZ (2.18 shoots), and Gamborg B5 medium supplemented with 6.0 mg/l BAP (2.43 shoots). The maximum average number of the Putih cultivar shoots was obtained on MS supplemented with 2.0 mg/l BAP (3.57 shoots). MS basal media was used for root initiation, as it produced an average of 25 roots with an 11-cm length. Various types of substrate mixtures were used during acclimatization. The best acclimatization substrate for the Wangi cultivar was 100% peat soil, whereas the Putih cultivar grew optimally in a combination of peat and perlites at a 1:1 ratio. Taro plantlets require approximately 4 to 6 weeks to acclimatize before they can be transferred to the field.

• Journal of Ecology and Environment
• /
• v.46 no.3
• /
• pp.218-242
• /
• 2022

Background: Promising specific growth regulators are employed in the tissue cultures of various bamboo species. Specific natural hardening mixtures support the acclimatization and adaptation of bamboo under protected cultivation. Results: The growth regulators like 2, 4-Dichlorophenoxyacetic acid (2, 4-D), Naphthaleneacetic Acid (NAA), Thidiazuron (TDZ), 6-Benzylaminopurine (BAP), Kinetin, Gelrite, Benzyl Adenine (BA), Indole Butyric Acid (IBA), Coumarin, Putrescine, Gibberellic acid (GA₃), Indole Acetic Acid (IAA) has been widely used for callus induction, root regeneration and imposing plant regeneration in various species of bamboo such as Bambusa spp. and Dendrocalamus spp. Different combinations of growth regulators and phytohormones have been used for regenerating some of the major bamboo species. Natural hardening materials such as cocopeat, vermicompost, perlite, cow dung, farmyard manure, compost, soil, garden soil, and humus soil have been recommended for the acclimatization and adaptation of bamboo species. Standard combinations of growth regulators and hardening mixtures have imposed tissue culture, acclimatization, and adaptation in major bamboo species. Conclusions: Bamboo contributes to soil fertility improvement and stabilization of the environment. Bamboo species are also involved in managing the biogeochemical cycle and have immense potential for carbon sequestration and human use. This paper aims to review the various growth regulators, natural mixtures, and defined media involved in regenerating major bamboo species through in vitro propagation. In addition, the ecological benefits of safeguarding the environment are also briefly discussed.

• Korean Journal of Plant Resources
• /
• v.36 no.6
• /
• pp.588-596
• /
• 2023

Long-term culture of cell lines is an important issue in in vitro culture and in plant science. In this study, the regeneration ability and ex vitro acclimatization of regenerants were evaluated. The ploidy level of regenerants derived from long-term cultured cell lines was measured in Imperata cylindrica 'Rubra', Poaceae. Adventitious buds (shoots) were successfully induced from five-year-cultured calli on MS medium containing 0.1 mg/L BA or 2.0 mg/L TDZ, combined with 0.01 mg/L auxins (IAA, IBA, NAA and 2,4-D), respectively. Adventitious roots were also induced on MS medium containing 0.01 mg/L auxins (IBA, NAA and 2,4-D), respectively. Interestingly, regenerants with both red and green leaf were successfully obtained when regenerants were cultured on MS medium with 9% sucrose. Regenerants derived from long-term cultured calli were transferred to pots using an optimal acclimatization process and successfully adapted to both pot and soil conditions. Moreover, the ploidy level was measured using calli and regenerants that had been kept on MS medium containing various kinds of plant growth regulators (PGRs).

• Korean Journal of Medicinal Crop Science
• /
• v.27 no.6
• /
• pp.397-403
• /
• 2019

Background: Oplopanax elatus is widely distributed at high altitudes (about 1,100 m) in China, Russia and Korea. It is hard to propagate, breed, and difficult to grow. Hence, it has been designated as a rare and endangered medicinal plant. A study was conducted to establish a system for large scale seedling production of Oplopanax elatus in vitro and to find the ideal environment for its seedling growth. Methods and Results: In this study, the explants produced under in vitro conditions during our previous study were grouped into three categories (under 10 mm, 10 mm - 30 mm and above 30 mm) based on plant height and were transferred to the growth-chamber and greenhouse for two weeks in each setting for acclimatization. The plantlet category of above 30 mm showed good performance, and was further evaluated under three acclimatization methods as follows: three different growth media (commercial soil, commercial soil + perlite, commercial soil + sand), four shading levels (0%, 50%, 70%, 90%) and four altitude levels (157 m, 218 m, 601 m, 870 m) in Gangwon province of South Korea. As results, O. elatus seedlings showed better growth characteristics at 870 m of altitude, 70% shading level and in the commercial soil compared to other treatments. Conclusions: The regenerated seedlings of Oplopanax elatus obtained through plant tissue culture would be advantageous for use in large scale seedling production systems paired with a good acclimation method. For obtaining optimal results, it is recommended that seedling be acclimatized in a high altitude environment.

• Asian-Australasian Journal of Animal Sciences
• /
• v.18 no.10
• /
• pp.1445-1450
• /
• 2005

This experiment was conducted to evaluate the effect of feed withdrawal (F) and heat acclimatization (A) on malebroiler and -layer chickens responses to acute heat stress (AHS) at four weeks of age. Totals of ninety male chicks of broiler or layer type were randomly allocated into 30 pens of grower batteries with raised wire floors. Chicks were subjected to F and A three times a week through the first three weeks of age. At each time, feed withdrawal and heat acclimatization (T = $35^{\circ}C$) lasted for six and four hours, respectively. Feed consumption (FC), body weight (BW), and feed conversion ratio (FCR) were recorded weekly for broiler type chickens only. At four weeks of age, all groups of chickens were exposed to AHS (T = $39{\pm}1^{\circ}C$) for three hours. Before and after AHS challenge, body temperature (Tb), heterophil (H), and lymphocyte (L) counts were recorded, and H/L ratio was calculated. Antibody (Ab) response to sheep red blood cells (SRBC) was assessed from all treatments without being exposed to AHS. Group F of broiler-type chickens weighed less (p<0.05) compared to control group. Also, both A and F groups of broiler-type chickens consumed less (p<0.05) feed when compared to control group. Acute heat stress elevated Tb of all treatment groups, however the increase was more profound (p<0.001) in broiler chicks. Broiler chicks of both A and F groups showed a tendency to have higher (p = 0.08) Tb when compared to control group. Acute heat stress elevated (p<0.001) H/L ratio in both types of chickens. Broiler chicks maintained higher (p<0.001) H/L ratio. Both F and A groups reduced (p<0.01) the level of elevation in H/L ratio compared to control groups of both types of chickens. Neither A nor F group affected the Ab production in response to SRBC. However, there was a tendency towards higher Ab responses in F group when compared to other groups in both types of chickens. Results of the present study demonstrate that previous history of feed withdrawal or episodes of heat exposures improved chicks'physiological withstanding of AHS and a tendency to improved humoral immune response.