• Plant Biotechnology Reports
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• v.5 no.3
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• pp.245-254
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• 2011

This study describes an efficient protocol for Agrobacterium tumefaciens-mediated transformation of two subgroups of genotype AAA bananas (Musa acuminata cv. Pei Chiao and Musa acuminata cv. Gros Michel). Instead of using suspension cells, cauliflower-like bud clumps, also known as multiple bud clumps (MBC), were induced from sucker buds on MS medium containing $N^6$-Benzylaminopurine (BA), Thidiazuron (TDZ), and Paclobutrazol (PP333). Bud slices were co-cultivated with A. tumefaciens C58C1 or EHA105 that carry a plasmid containing Arabidopsis root-type ferredoxin gene (Atfd3) and a plant ferredoxin-like protein (pflp) gene, respectively. These two strains showed differences in transformation efficiency. The EHA105 strain was more sensitive in Pei Chiao, 51.3% bud slices were pflp-transformed, and 12.6% slices were Atfd3-transformed. Gros Michel was susceptible to C58C1 and the transformation efficiency is 4.4% for pflp and 13.1% for Atfd3. Additionally, gene integration of the putative pflp was confirmed by Southern blot. Resulting from the pathogen inoculation assay, we found that the pflp transgenic banana exhibited resistance to Fusarium oxysporum f. sp. cubense tropical race 4. This protocol is highly advantageous to banana cultivars that have difficulties in setting up suspension cultures for the purpose of quality improvement through genetic transformation. In addition, this protocol would save at least 6 months in obtaining explants for transformation and reduce labor for weekly subculture in embryogenic cell suspension culture systems.

• Proceedings of the Korean Magnestics Society Conference
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• 2007.05a
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• pp.306.1-306.1
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• 2007

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.9
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• pp.4302-4309
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• 2012

Underground structures without the expansion joint in the settled intervals, the underground structures may suffer from structural crack and the water leakage thereby resulting in the occurance of the efflorescence. In this study, the performance of new expansion joint used in underground structures were verified. The spacing of expansion joint was defined by the finite element analysis. Expansion length, resistance and waterproofing performance of developed expansion joint were confirmed by experiment.

• Korean Management Science Review
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• v.29 no.3
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• pp.13-21
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• 2012

As culture technology is recognized as the important element of national industry, the necessity of the related policy development for enhancement of culture technology is magnified. In this study, we use conjoint analysis to investigate how the researchers from industry and academia think differently on the importance of several attributes in government-supported culture technology research project such as research period, size of research fund, inter-affiliated research requirement and interdisciplinary research requirement, and preference on those attribute levels. Also, we describes how a simulation experiment is designed to simulate participation shares of four alternative hypothetical research project profiles. This study will help establish the more effective policy of the national research program by understanding the preferential differences between industry and academia on the research project attributes.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.1
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• pp.104-109
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• 2009

This paper designed 3D animation in consideration of young children's characteristics to stimulate their interest and to be more understandable to them. It was also confirmed that, using the 3D animation software, young children came to recognize the problem of environment pollution and developed desirable attitude and behavior fur protecting the environment. Furthermore, this paper suggested the derection of systematic environmental education at kindergartens and other early childhood education institutions using the developed 3D animation software as a leaching material.

• Proceedings of the Korean Society for Language and Information Conference
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• 2007.11a
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• pp.136-145
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• 2007

This paper explains how we define and represent modality in E-HowNet. Following Lyons (1977, reviewed in Hsieh 2003, among others), we hold that modals express a speaker's opinion or attitude toward a proposition and hence have a pragmatic dimension and recognize five kinds of modal categories, i.e. epistemic, deontic, ability, volition and expectation modality. We then present a representational formalism that contains the three most basic components of modal meaning: modal category, positive or negative and strength. Such a formula can define not only modal words but also words that contain modal meanings and cope with co-compositions of modals and the negation construction.

• Proceedings of the Korean Vacuum Society Conference
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• 2001.07a
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• pp.152-152
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• 2001

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.2
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• pp.689-695
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• 2010

The effect of the code conversion process of Korean script (Hangul), also known as words for numbers, was investigated using event-related potentials (ERPs) during mental arithmetic operations. Study subjects were asked to determine whether the arithmetic results of a given target stimuli were correctly matched. Visual inspection and statistics of mean ERPs showed stimulus type-dependent processing rather than task-dependent processing. Results of addition and multiplication tasks revealed that the overall temporal profiles of the Arabic numerals were similar to the Hangul words for numbers. The only exception to this observation was a delayed positive-slope peak occurring around 300 ms, which was likely related to the encoding process of Hangul words for numbers to Arabic-digits, defined as a 'transcoding-related potential.' Source analysis confirmed that the topography of different waveforms for the two conditions was attributed to a single dipole located in the left temporo-parietal area; this area is known to be involved in Hangul words for number processing. These results suggest that the initial processing for encoding words for numbers was followed by arithmetic operations without direct access of internal number representation. Korea Academia-Industrial cooperation Society. The Korea Academia-Industrial cooperation Society. The Korea Academia-Industrial cooperation Society. The Korea Academia-Industrial cooperation Society.

• Journal of Advanced Navigation Technology
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• v.27 no.6
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• pp.690-698
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• 2023

Information sharing and decision making between airport stakeholders became possible after the introduction of airport cooperative making system (A-CDM). This also resulted in optimizing aircraft handling time and increased the efficiency of aircraft operations. Technological advances have recently led to the development of urban air mobility (UAM) which is a small aircraft taking off and landing vertically. It is emerging as a new air transportation system in the future due to its advantage of saving time and solving congestion problem in the urban area. This study aims to suggest how vertiport cooperative decision making system (V-CDM) should be managed for efficient operation of UAM. By establishing procedure for decision making system based on Vertiport ecosystem of UAM. By establishing procedure for decision making system based on Vertiport ecosystem and UAM aircraft, unnecessary flight delays or cancellations can be minimized and efficiency of UAM operation will be improved as well.

• Proceedings of the Korean Magnestics Society Conference
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• 2007.05a
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• pp.319.2-319.2
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• 2007