In order to study the ecotypic variation of Robinia pseudo-acacza L. distributed in southern area of Korean peninsula, 15 local populations(Daejin, Sokcho, Kangneung, Mt. Surak, Hongcheon, Kwangneung, Namhansanseong, Chungju, Yesan, Andong, Jeonju, Dalseong, Changweon, Mokpo and Wando), located from $34^{\circ}18'N\;to\;38^{\circ}36'N$, were selected based on their latitudes and geographical distances. Seeds of these populations were collected and their germination and growth of seedlings were investigated. The optimum temperature of seed germination tends to decrease with ascending latitudes. The optimum temperatures of local populations were classified into three types: the north central type of $25^{\circ}C$(Daejin, Sokcho, Kangneung, Mt. Surak, Hongcheon, Kwangneung), the south central type of $30^{\circ}C$(Narnhansanseong. Chungju, Yesan, Andong, Jeonju), and the southern type of $35^{\circ}C$(Dalseong, Changweon, Mokpo, Wando). The optimum temperature for the growth of seedlings was $25^{\circ}C$ for all populations. The populations were also classified into three types : north central type, south central type and southern type based on the ranking on the growth of seedlings at 5 different temperatures of 15, 20, 25, 30 and $35^{\circ}C$, and the results of seedling growth showed similar patterns to those of the seed germination.