• 한국환경보건학회지
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• 제43권5호
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• pp.360-369
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• 2017

Objectives: The organizational culture of small-scale enterprises is influenced by a lack of awareness of industrial accidents and a business-oriented culture. Therefore, the purpose of this study is to establish a correlation between chemical awareness and worker safety, which is the reason why a company's safety environment is responsible for human factor accidents. Methods: The research model established the organizational culture, human relationships, organizational relationships, workplace culture, safety climate, and safety parameters, all of which affect the perception of chemical substances, then established safety rankings, communication, safety regulations, and safety support. Results: In order to derive the results of this study, the satisfaction level was mostly derived from the degree of engagement (+) depending on the number of years worked, in the hierarchical regression analysis. During work with chemicals, the number of years spent on the level of the service was determined by the influence of the chemical (-). Based on this company, the occurrence of chemical accidents caused by chemical materials was felt to be felt at the risk of causing the hazard to be felt, the absence of physical damage was excluded from the variables. The other variables detected in the variables were $62.8{\pm}24.4$. Conclusion: As a result, it is necessary to develop a statistical model for the verification of the hierarchical regression analysis, and the relationship between safety and awareness of the dangers of chemicals and biological agents requires periodic safety training. Job satisfaction was found to be higher if the level of awareness was sufficient.

• ALGAE
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• 제28권2호
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• pp.193-202
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• 2013

Nitrogen availability and cell density each affects growth and cellular astaxanthin content of Haematococcus pluvialis, but possible combined effects of these two factors on the content and productivity of astaxanthin, especially under outdoor culture conditions, is less understood. In this study, the effects of the initial biomass densities IBDs of 0.1, 0.5, 0.8, 1.5, 2.7, 3.5, and 5.0 g $L^{-1}$ DW and initial nitrogen concentrations of 0, 4.4, 8.8, and 17.6 mM nitrate on growth and cellular astaxanthin content of H. pluvialis Flotow K-0084 were investigated in outdoor glass column photobioreactors in a batch culture mode. A low IBD of 0.1 g $L^{-1}$ DW led to photo-bleaching of the culture within 1-2 days. When the IBD was 0.5 g $L^{-1}$ and above, the rate at which the increase in biomass density and the astaxanthin content on a per cell basis was higher at lower IBD. When the IBD was optimal (i.e., 0.8 g $L^{-1}$), the maximum astaxanthin content of 3.8% of DW was obtained in the absence of nitrogen, whereas the maximum astaxanthin productivity of 16.0 mg $L^{-1}\;d^{-1}$ was obtained in the same IBD culture containing 4.4 mM nitrogen. The strategies for achieving maximum Haematococcus biomass productivity and for maximum cellular astaxanthin content are discussed.

• Parasites, Hosts and Diseases
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• 제49권3호
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• pp.295-298
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• 2011

Lactobacillus species in the female genital tract are thought to act as a barrier to infection. Several studies have demonstrated that lactobacilli can adhere to vaginal epithelial cells. However, little is known about how the adherence of lactobacilli to vaginal epithelial cells affects the acidity, cell viability, or proliferation of the lactobacilli themselves or those of vaginal epithelial cells. Lactobacillus acidophilus was co-cultured with immortalized human vaginal epithelial cells (MS74 cell line), and the growth of L. acidophilus and the acidity of the culture medium were measured. MS74 cell density and viability were also assessed by counting cell numbers and observing the cell attachment state. L. acidophilus showed exponential growth for the first 6 hr until 9 hr, and the pH was maintained close to 4.0-5.0 at 24 hr after culture, consistent with previous studies. The growth curve of L. acidophilus or the pH values were relatively unaffected by co-culture with MS74 cells, confirming that L. acidophilus maintains a low pH in the presence of MS74 cells. This co-culture model could therefore potentially be used to mimic vaginal conditions for future in vitro studies. On the other hand, MS74 cells co-cultured with L. acidophilus more firmly attached to the culture plate, and a higher number of cells were present compared to cells cultured in the absence of L. acidophilus. These results indicate that L. acidophilus increases MS74 cell proliferation and viability, suggesting that lactobacilli may contribute to the healthy environment for vaginal epithelial cells.

• 한국가정과교육학회지
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• 제32권1호
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• pp.51-76
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• 2020

본 연구는 2015 개정 고등학교 가정과 교육과정에 적용할 수 있는 전통 생활 문화 교육 프로그램을 개발해 제안하는 것이다. 가정 교과는 전통 생활 문화를 교과 내용으로 다루고 있으며, 현대생활에 적합한 새로운 문화를 재창조할 수 있는 능력을 함양하는 것에 목적을 둔다. 기존 학교에서의 전통 생활 문화 교육은 이론 교육 위주 혹은 일회성의 체험 위주의 교육이 이루어져 왔다. 또한 주로 초등학생과 중학생 대상의 프로그램이 개발되어, 고등학생을 위한 프로그램은 부족한 실정이다. 이에 본 연구는 고등학생을 위한 가정과 프로그램을 개발해 전통 문화 교육의 발전적 방향을 제시하고자 하였다. 이를 위해 한식, 한복, 한옥에 공통적으로 다룰 수 있는 전통 문양과 오방색을 전통 생활 문화 교육 주제로 선정하고, ADDE의 단계를 따라 전통 생활 문화교육 프로그램을 개발하였다. 분석 단계에서는 2015 개정 고등학교 가정과 교육과정과 12종 가정 교과서의 만들기 활동, 교사와 학습자의 생활 문화 교육에 대한 경험과 요구를 분석하였다. 설계 단계에서는 프로그램 개발을 위한 스토리텔링 기반 메이커 교육 모형 설계와 수업 주제 도출, 만들기 활동, 메이커 도구와 재료를 선정하였다. 개발 단계에서는 전통 의·식·주 생활문화 교육을 위한 프로그램을 개발하였다. 평가 단계에서는 가정과 교육 전문가 12명을 대상으로 타당도 검사 및 피드백을 받아 수정하여 프로그램을 완성하였다. 추후 연구로 이 프로그램을 적용해 학생들의 전통 생활 문화에 관한 인식과 전통 생활문화의 발전과 계승 의지 등의 효과를 살펴보는 연구를 제언한다.

• Reproductive and Developmental Biology
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• 제32권3호
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• pp.167-173
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• 2008

Somatic cells such as oviduct epithelial cell, uterine epithelial cell, cumulus-granulosa cell and buffalo rat river cell has been used to establish an effective culture system for bovine embryos produced in in vitro. But nitric oxide (NO) metabolites secreted from somatic cells were largely arrested the development of bovine in vitro matured/ in vitro fertilized (IVM/IVF) embryos, suggesting that NO was induced the embryonic toxic substance into culture medium. The objective of this study was to investigate whether BOEC co-culture system can ameliorate the NO-mediated oxidative stress in the culture of bovine IVM/IVF embryos. Therefore, we evaluated the developmental rate of bovine IVM/IVF embryos under BOEC co-culture system in the presence or absence of sodium nitroprusside (SNP), as a NO donor, and also detected the expression of growth factor (TGF-$\beta$, EGF and IGFBP) and apoptosis (Caspase-3, Bax and Bcl-2) genes. The supplement of SNP over 5 uM was strongly inhibited blastocyst development of bovine IVM/IVF embryos than in control and 1 uM SNP group (Table 2). The developmental rates beyond morulae stages of bovine IVM/IVF embryos co-cultured with BOEC regardless of SNP supplement (40.4% in 5 uM SNP+ BOEC group and 65.1% in BOEC group) were significantly increased than those of control (35.0%) and SNP single treatment group (23.3%, p<0.05: Table 3). The transcripts of Bax and Caspase-3 genes were detected in all experiment groups (1:Isolated fresh cell (IFC), 2:Primary culture cell (PCC), 3:PCC after using the embryo culture, 4: PCC containing 5 uM SNP and 5: PCC containing 5 uM SNP after using the embryo culture), but Bcl-2 gene was not detected in IFC and PCC (Fig. 1). In the expression of growth factor genes, TGF-$\beta$ gene was found in all experimental groups, and EGF and IGFBP genes were not found in IFC and PCC (Fig. 2). These results indicate that BOEC co-culture system can increase the development beyond morula stages of bovine IVM/IVF embryos, possibly suggesting the alleviation of embryonic toxic substance like nitric oxide.

• Reproductive and Developmental Biology
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• 제30권2호
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• pp.135-141
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• 2006

The present study was conducted to investigate the effects of cumulus cells and porcine follicular fluid (pFF) on plasminogen activator (PA) activity and oocytes maturation in vitro in the pig. The cumulus-oocyte complexes (COCs) and denuded oocytes (DOs) were incubated in NCSU-23 medium with or without 10% pFF for 0, 24, or 48 hr. In the presence of cumulus cells, the proportions of oocytes matured to metaphase-II stage were significantly (P<0.05) higher in medium with pFF than without pFF (69.8 vs. 37.7%, respectively). When COCs and DOs were cultured in the presence of pFF, tissue-type PA (tPA), urokinase-type PA (uPA), and tPA-PA inhibitor (tPA-PAI) were observed in COCs, and PA activities were higher at 48 hr than 24 hr. When COCs and DOs were cultured in the absence of pFF, tPA and tPA-PAI were observed in COCs, and PA activities were increased as duration of culture increased. No PA activities were detected in DOs regardless of pFF supplementation. When porcine oocytes were cultured in the presence of pFF for 24 and 48 hrs, the activities of tPA-PAI, tPA, and uPA were observed in both COCs and DOs. In medium of absence of pFF, PA activities were observed in oocytes with cumulus cells only. On the other hand, three plasminogen-dependent lytic bands (tPA-PAI, tPA, and uPA) were observed in pFF cultures. Particularly uPA activity was higher than the other kinds of PA activity. When oocytes and cumulus cells were separated from porcine COCs at 0 hr of culture, tPA-PAI, tPA, and uPA were detected in cumulus cells at 48 hr of culture, but no PA activities were in DOs. The presence of pFF and cumulus cells in maturation medium stimulated not only nuclear and cytoplasmic maturation in porcine COCs, but also PA production by cumulus cells and COCs. It is possible that PAs produced by cumulus cells migrated through the gap junction between oocyte and cumulus cells. These results suggest that porcine oocytes have no ability to produce PA themselves.

• KSBB Journal
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• 제18권5호
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• pp.371-376
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• 2003

프로바이오틱 생균인 B. polyfermenticus SCD의 유용성을 확인하기 위해 항산화 및 콜레스테롤 저하 효과를 검증하였다. DPPH법에 의한 항산화 활성은 48% 정도였으며, TCA법에 의한 활성은 45%로 측정되었다. 그리고 TBARS 법에 의해 측정된 활성은 stimulator가 존재할 때 60%에 달하였으며, SOD 유사활성은 15% 정도였다. 이러한 활성은 기존의 항산화제인 BHT나 $\alpha$-tocopherol의 1% 용액과 비교할 때, 비슷하거나 약간 낮은 수치를 보여 준다. 향후 항산화 물질이 정제되어 이 물질의 1% 용액과 1% 농도의 기존 항산화제의 활성을 비교하여야 한다. 그러나 이 같은 뚜렷한 B. polyfermenticus SCD의 항산화력을 바탕으로 천연 항산화제 개발이 가능하다고 판단된다. 한편 B. polyfermenticus SCD의 콜레스테롤 저하 효과는 0.3% oxgall이 존재할 때, 64% 정도였다. 이 수치는 oxgall이 존재하지 않을 때 수치(67%)와 비교할 때, 큰 변화가 없었다. 그리고 콜레스테롤 저하 기작은 세포내 흡착에 의한 것으로 판단되었다.

• Safety and Health at Work
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• 제11권4호
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• pp.450-457
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• 2020

Background: The production of glass beads in Ghana is greatly impacted by the ingenuity of Ghanaian women. Preliminary investigations revealed the lack of interest on the part of women due to poor working conditions as a result of the influence of culture-specific silence. Therefore, the study investigated the poor working conditions faced by these industrious women with the ultimate goal of suggesting ways they can be empowered. Methods: A phenomenological study was conducted in two indigenous glass bead communities in Ghana. Data were solicited via direct observations, personal interviews and focus group discussions. Twenty-six purposively sampled respondents were recruited for the study. Data from the study were analyzed using Interpretative Phenomenological Analysis. Results: The results confirmed that the elderly women glass bead makers are much influenced by the Ghanaian culture of silence. This prevents the women from speaking about the challenges they are facing in their work. Also, the women are silenced because of the fear of losing their jobs as well as the reluctance of their male managers to remedy the challenges they encounter in the course of work. This has resulted in poor remuneration, lack of insurance packages for workers, certification, and absence of personal protective tools for the women. Conclusion: The study tasks the government of Ghana, the Legal Advocacy for Women in Africa (LAWA), the Fair Wages and Salaries Commission in Ghana, the Ghana Trade Union as well as the Local Government Workers' Union to empower the women to sustain the glass bead industry in Ghana.

• 한국발생생물학회지:발생과생식
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• 제10권1호
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• pp.63-73
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• 2006

간질환 환자의 대부분은 간 조직 손상으로 인해 간세포의 재생 능력이 감소한다. 간세포 이식은 이러한 간질환을 치료하는데 있어 혁신적인 방법으로 대두되고 있으나, 여전히 많은 의문과 문제점이 제기되고 있다. 사람의 양막으로부터 얻은 줄기 세포를 이용하여 간세포 분화를 위한 최적의 조건을 알아 보고자 하였다. 세포내 알부민에 대한 면역 화학적 방법, 세포내 글리코겐의 특이 염색법, 세포의 형태적 변화 연구 방법 등을 이용하여 여러가지 배양 조건을 조사한 결과, 배양 접시를 fibronectin으로 coating하고 배양액내에 insulin/transferrin/selenium(ITS)을 첨가하는 것이 양막 줄기세포의 간세포로의 분화에 효과적이었다. 또한 배양액내에 fibroblast growth factor(FGF)-1과 FGF-2를 함께 첨가하는 것이 둘 중 하나만 첨가하거나 첨가하지 않는 것보다 효과적이었다. 한편 분화 배양은 한가지 배양액을 사용한 지속적인 배양법(continuous culture method)보다 배양 조건을 달리하여 두 단계로 배양하는 2단계 배양법(two-step culture method)가 훨씬 효과적이었다. 마지막으로, 기본 배양액에 FGF-2와 FGF-4를 첨가한 조건과 FGF-4와 $TGF-{\alpha}$를 첨가한 조건이 다른 조건 보다 알부민 분비를 많이 하는 것으로 보아 FGF-4가 간세포 분화 과정에 중요한 역할을 하는 것으로 여겨지며 FGF-2 및 $TGF-{\alpha}$ 첨가는 더욱 효과적인 배양 조건으로 관찰되었다. 따라서, 양막에서 유래한 성체 줄기 세포는 적절한 배양 조건이 주어질 때, 간세포로 분화가 가능하며, 분화 과정에서 FGF-4가 주도적인 역할을 하며 FGF-2와 $TGF-{\alpha}$는 상승 효과를 갖는 것으로 사료된다.

• 한국철학논집
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• 제30호
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• pp.311-344
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• 2010

중국 대륙에서 전통문화(傳統文化)와 사상(思想)은 근 100여년의 시간동안 역사의 격변을 거치면서 타도의 대상에서 계승해야할 유산(遺産)으로 탈바꿈 되었다. 오늘날 중국 정부와 학계, 민간에서는 빈부격차, 가치관의 부재, 정신적 의지처의 상실 등 경제발전이 남긴 부작용을 유학(儒學)을 중심으로 한 전통사상과 문화의 복원을 통해 해결하고자 한다. 중국 정부는 표면적으로는 사회주의 이론체계들을 고수하면서도 유학(儒學)을 이용하여 사회적 안정과 통합을 도모하고 지속적인 경제성장을 이룰 수 있는 버팀목으로 삼고자 한다. 민간의 경우 경제발전을 통해 민족적 자신감을 회복하게 되자 전통문화 복원과 선전을 통해 민족적 자부심을 고취하고 중국문화를 세계에 선양하려는 노력이 이루어지고 있다. 특히 전통문화와 사상의 복원을 주장하는 학자들은 전통 속에서 문화적 통일성과 정신적 안식처 그리고 새로운 가치체계를 모색하고자 한다. 비판적 계승론자나 당대 문화보수주의자들은 전통문화와 사상이 현대화에 기여할 수 있는 요소를 지니고 있으며, 전통사상에 대한 새로운 이해와 해석을 통해 현대화의 부작용을 해결해 나갈 수 있다고 주장한다. 우리의 입장에서 본다면, 전통과 문화를 일종의 '역량(力量)'(power)으로 간주하는 대륙의 비판적 계승론자나 문화보수주의자들의 관점에 대한 심도 있는 이해가 필요하며, 우리에게도 전통문화와 사상의 새로운 해석을 통해 현실문제 해결의 계기를 모색하려는 적극적인 노력이 요청된다고 할 수 있다.