• Clinical and Experimental Reproductive Medicine
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• 제21권2호
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• pp.183-190
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• 1994

Purpose of the present study was to find the optimal culture conditions for the maturation and fertilization of immature oocytes by the use human body fluids and gonadotropins (Gn) in the mouse model. Cumulus-enclosed mouse immature oocytes were incubated in the medium containing various human body fluids with or without Gn in vitro, and examined to confirm nuclear maturation (NM) and fertilization. Female ICR mice were stimulated with 7.5 IU pregnant mares' serum gonadotropin (PMSG). Cumulus-enclosed immature oocytes were isolated at 48-52 hr post PMSG injection and cultured in TCM 199 supplemented with various concentrations (20, 50, and 70%) of human body fluids such as fetal cord serum (hCS), follicular fluid (hFF), peritoneal fluid (hPF) and amniotic fluid (hAF) in the presence or absence of 10 IU/ml PMSG and 10 IU/ml human chorionic gonadotropin (hCG) for 18 hr. Fetal calf serum (FCS) was used as a control for the supplements. Matured oocytes were fertilized with sperm collected from the epididymis of male mice. Fertilization was conducted in T6 medium containing 15 mgl ml bovine serum albumin, and confirmed at 6 hr post-insemination. Evaluation of nucler maturation and fertilization was carried out by rapid staining using fuchin. There was no significant difference between the effects of human body fluids and FCS supplements on nuclear maturation of cumulus enclosed mouse immature oocytes. When maturation medium was supplemented with 20% hPF or 20% hAF, fertilization rates were significantly (P<0.01) lower than that of 20% FCS, hCS and hFF groups. However, higher concentrations of body fluids during IVM were not more beneficial on fertilizability of oocytes. The addition of Gn significantly increased the fertilization rates in hPF and hAF groups (hPF without Gn; 51.5%, compared with 85.1% for addition of Gn, and hAF without Gn; 30.1% compared with 85.8% for addition of Gn) at 20% concentration. These results suggest that human body fluids at 20% concentration and gonadotropins can be used as supplements for the maturation of mouse immature oocytes in vitro. When gonadotropins supplemented with the human body fluids in the maturation medium, fertilizability of mouse immature oocytes was increased in hPF and hAF groups. These results can be applied to maturation of human immature oocytes in vitro.

• 한국조리학회지
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• 제10권3호
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• pp.97-118
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• 2004

As the economy grows rapidly and the national income level increases, the service industry has become more important and its size gets larger. Especially, the food industry undertakes a continuing growth of consumer expenditures through no nationalization of food, consumers varying patterns of eating out, and individualization. However, it includes many problems with improving service quality towards customers owing to the absence of systems and philosophy to realize customer satisfaction management Therefore, this study conducted a study with Korean traditional restaurant customers to measure their perceived service quality, to verify what factors most influence consumer satisfaction, and to suggest ways to meet the costumer needs by integrating the study results and developing high service quality. To sum up the results of this study, Korean traditional restaurant visitors evaluated three dimensions of service quality overall favorably. Interestingly, background music received a lower level of satisfaction. This implies that the Korean traditional restaurant managers do not do a fine performance in managing music as an ambient element to evoke the atmosphere of the restaurant. Customers showed a lower satisfaction with menu price among the factors measuring product quality. As a result of this study, several suggestions have been made as follows: First, it is necessary for the Korean traditional restaurants to provide systematic employee training to instigate a service culture of customer focus. Secondly, it is important to develop traditional foods using ingredients produced in the region based on a literature review of food in Gyeongju, Silla. Thirdly, it is an urgent task to develop regional menus to revitalize the restaurant business. Fourthly, it is important to provide safety food through systematic sanitary administration. Lastly, it is necessary to limit the number of menu items and attempt specialization of them. Such an attempt with specialized menu items will help their food taste and quality improve and thereby reduce inventory burdens. However, this study has a few limitations. Since this study conducted a survey of the restaurants which provides only Korean traditional food in Gyeongju, not all the restaurants in it, there is a limitation in measuring customer satisfaction with service quality. Therefore, the study results cannot be generalized to all the restaurants in Gyeongju and the nation. Finally, this study suggests that studies on the relationships between customer satisfaction and menu price and customer value system or further customer satisfaction studies with Korean traditional restaurants should be continued.

• IMMUNE NETWORK
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• 제6권1호
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• pp.33-42
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• 2006

Background: Calcineurin plays a crucial role in T cell activation, cell growth, apoptosis, and angiogenesis, and its over-expression has been implicated in the pathogenesis of cardiomyopathy and stroke. However, the expression and function of calcineurin in the pathologic lesion of chronic inflammatory diseases, like rheumatoid synovium, remain to be defined. This study was aimed to determine the role of calcineurin in inflammatory arthritis and investigate the expression and function of calcineurin in the rheumatoid synovium and synoviocytes, the actual site of chronic inflammation. Methods: Immuno-histochemical staining using specific antibody to calcineurin was perfomed in the synovium of rheumatoid arthritis (RA). Fibroblast-like synoviocytes (FLS) from RA and osteoarthritis (OA) patients were isolated from RA and OA patients, and cultured with IL-1${\beta}$ and TNF-${\alpha}$ in the presence or absence of cyclosporin A, a calcineurin inhibitor. The calcineurin expression was assessed by phosphatase assay and Western blotting analysis. IL-6, -10, -17, matrix metalloproteinase (MMP)-1, -2, -3, and -9 released into the culture supernatants were measured by ELISA. After transfection with GFP-Cabin 1 gene into synoviocytes, the levels of IL-6 and MMPs were measured by ELISA. Results: Calcineurin was highly expressed in the lining layer of synovium and cultured synoviocytes of RA patients. The elevated calcineurin activity in the rheumatoid synoviocytes was triggered by proin flammatory cytokines such as IL-1${\beta}$ and TNF-${\alpha}$. In contrast, IL-10, an anti-inflammatory cytokine, failed to increase the calcineurin activity. The targeted inhibition of calcineurin by the over-expression of Cabin 1, a natural calcineurin antagonist, inhibited the production of IL-6 and MMP-2 by rheumatoid synoviocytes in a similar manner to the calcineurin inhibitor, cyclosporin A. Conclusion: These data suggest that abnormal activation of calcineurin in the synoviocytes may contribute to the pathogenesis of chronic arthritis, and thus provide a potential target for controlling inflammatory arthritis.

• 한국조경학회지
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• 제38권4호
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• pp.45-53
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• 2010

본 연구는 유비쿼터스 환경에서 참여형 환경조형물의 이용자 만족도에 관한 연구를 통하여 앞으로 유비쿼터스 시대에 이용자들이 사용하게 될 환경조형물에 대한 만족도의 체감을 높이기 위한 사용성과 서비스, 기능성, 조형성에 대한 유비쿼터스 도시 공간 속에서의 U-환경조형물의 좀 더 발전적인 방향을 제시하고자 강남대로 U-Street에 설치되어진 U-환경조형물인 미디어 폴을 조사 대상지로 선정하였다. 연구결과를 요약하면 다음과 같다. 유비쿼터스 환경에서 시작단계의 환경조형물인 미디어 폴의 부문별 만족도 중에서 서비스 만족도, 기능성 만족도, 조형성 만족도와 미디어 폴의 종합적인 만족도로 설정한 후 조사 분석하여 만족도 연구를 수립하였다. 미디어 폴의 부문별 만족도에서 서비스 만족도는 대중교통 안내도, 기능성 만족도는 거리문화의 촉매제, 조형성 만족는 '형태성', 미디어 폴의 부문별 만족도와 종합 만족도 관계에서 기능성 만족도가 가장 큰 영향을 주는 것으로 나타났다. 이는 미디어 폴의 만족도에 가장 큰 영향을 주는 요인으로 나타난 만큼 도시공간에서 U-환경조형물의 만족도의 체감을 높이기 위한 미디어 폴의 다양한 컨텐츠 개발과 사용, 편리한 기술개발, 미디어 폴의 기능성 만족도에 대한 지속적 연구와 강남의 랜드 마크가 되기 위해서는 시민들과 소통하기 위한 즐거움과 감동을 줄 수 있는 스토리가 담긴 인간 중심의 기능성 요소 개발이 중요할 것이다. 현재 유비쿼터스의 초기단계는 가로 경관으로서 자연성이 결여, 환경조형물의 외피에 대한 개발, 인간에게 감동을 줄 수 있는 스토리텔링의 부재, 기술적 측면 부족, 수익성을 낼 수 있는 다양한 컨텐츠 개발, 투자에 비해 수익성 창출의 어려움, 사회소통 매개로서의 기능 등의 문제점을 보완해야 하며, 첨단 기술만이 아니라 경제적, 사회적, 문화적 측면을 고려한 똑똑한 기술보다는 편안하고 친인간적 기술로서 공간개발과 U-환경조형물의 설치가 요구되어야 할 것이다.

• 한국해양학회지
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• 제4권1호
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• pp.36-48
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• 1969

The larvae of Mytilus coruscus were grown at the room temperature of approximately 15.1C under several different sulture conditions, i.e., salinity, population of the larvae, density and kind of food organisms, etc. (1) The egg of Mytilus coruscus obtained in the laboratory measured about 73.0${\mu}$ in diameter. The embryos gradually developed into larvae up to 179.0${\mu}$ shell length with the shell height of 135.9${\mu}$ even in the absence of the algal food. Beyond this size, however, the growth of larvae was considerably retarded, indicating that the better growth could be expected if the food began to be fed four days after spawning. (2) The larvae began settling upon reaching 281.4${\mu}$ to 310.9${\mu}$ in shell length or 264.3${\mu}$ to 301.9${\mu}$ in shell height. When the shell length reaches 322.6${\mu}$ to 337.1${\mu}$, the shell height also reaches about the same, i.e., 321.5${\mu}$ to 346.2${\mu}$. (3) Daily rate of food consumption was determined by the size of the larvae and the species of the algal food. Regardless of the species of food given, the rage of food consumption remained almost the same until the larva reached the straight-hinge stage, and marked variations were found as the larvae grew larger. Daily rate of food consumption was shown as follow; Chaetoceros calcitrans : Y=2.99167e$\^$0.000018243x$\^$2// Cyclotella nana : Y=3.00324e$\^$0.000015481x$\^$2// Monochrysis lutheri : Y=3.000056e$\^$0.000014485$\^$2// (4) Suitable amount of the food to be given was about five times of the consumed food by Mytilus coruscus. (5) When the numbers of the larvae was higher than ten per milliliter, the growth was significantly retarded. (6) Monochrysis lutheri and Cyclotella nana were much better than Chaetoceros calcitrans as the food of Mytilus coruscus, and even the same food organism showed some difference with the age of the organism. (7) Sea water of higher salinity showed the better result in the growth of the larvae and the water with the specific gravity of 1.020 or below was dangerous for the larvae. (8) The mean growth of the larvae of Mytilus coruscus under lavorable condition was shown as follows: shell length 121.8${\mu}$ to 179.0${\mu}$ : Y=119.18+7.42X 196.7${\mu}$ to 322.6${\mu}$ : Y=203.144+7.687X 322.6${\mu}$ to 985.1${\mu}$ : Y=302.5978+11.8356X shell height 86.3${\mu}$ to 135.9${\mu}$ : Y=86.22+6.40X 158.1${\mu}$ to 321.5${\mu}$ : Y=162.998+10.027X 321.5${\mu}$ to 1,215.4${\mu}$ : Y=309.3701+16.258X Relationships between the shell length and shell height were shown as follows: shell length 121.8${\mu}$ to 179.0 : Y=0.83726X-15.79165 196.7${\mu}$ to 322.6 : Y=1.29909X-100.58610 322.6${\mu}$ to 985.1${\mu}$ : Y=1.3536X-101.6806

• 대한예방한의학회지
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• 제8권2호
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• pp.99-113
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• 2004

Problems and current situation of public health globally and domestically were analyzed in this study and based on these findings, ways to improve from western medicine and Oriental medicine can be deduced as follows: 1. Current problems of public health in Korea and the world 1) Increase of diseases resulted from daily habits and infectious diseases, many are at the brink of being ill. Quality of life from extended life span and unbalanced health care must be solved. 2) Natural and societal factors including host factors, public health service, and other external and internal factors play an important role in deciding healthy and being ill. 3) Some of the limits and problems of modem medicine include insufficient academic knowledge and incomplete theory, as well as misled approach to the treatment. Human itself isn't perfect organism and other realistic problems hinder one's well-being. 4) Regardless of western medicine or Oriental medicine, patients were approached as someone with diseases and disorders, and wholistic approach was disregarded. Lack of clinical training, absence of clear educational philosophy and goal are some of the reasons why the education isn't under concrete system 2. Important factors for the medical education and proper direction for the education of Oriental medicine 1) Important factors for medical education - Education should not be limited on the human health and illness, but also cover qualities such as well-being, social welfare, service, and happiness. Every aspects of human life must be considered and attended for more productive outcome. - Basic understanding of humanity must be included in the educational curriculum - Foundation of human diseases and pain are associated with inner life and surrounding causes including family, society, nature, race, culture, religion, politics, and etc., thus the education must be approached to recognize aforementioned criteria. 2) Proper direction for the education of Oriental medicine - Values of Oriental medicine for medical principles and importance of lifehood must be educated. - Educational goal, limits, and levels must be established for the school of Oriental medicine - Respect for life must be the top priorities of educational direction which should lead to solution based education for the human health. Latest medical theory and technology should be accommodated as well as prevention, treatment, and balancing of basic courses and clinical training for optimal education.

• 한국문헌정보학회지
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• 제11권
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• pp.147-183
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• 1984

This is an analytical study on Munheongak (文獻閣) and it's catalogue. The major objective of the study is to get a recognition of Munheongak under the culture of Sukjong (肅宗) period in Korean library history. Most of early studies made on such a category have been concentrated on Jiphyunjeon (集賢殿), Hongmungwan (弘文館), Kyujanggak(奎章閣) and their backgrounds. In this study, the author has invest gated Kungwolji (宮闕志), Munheongakseomg (文獻閣書目) and other materials related to this subject. The findings of the study can be summarized as follows: 1. Munheongak was established by king Sukjong in the 26th year of his reign. According to some records of Kungkwolji, the reason of establishment of the library was to arrange the collection in the Sango (相庫) consisted of various kinds of materials. In case of books, most of them turned out to be Korean books. 2. Munheongak was sited to the estern side of Kyunghyundang (景賢堂), which was located on the Kyungdeokgung (慶德宮). After Youngjo (英祖) the place was called Kyungheuigung (慶熙宮) so as to avoid the name of the precedent king. But these days, both the buildings are not to be found. 3. After its establishment, the library could not play the role as a library because of the then political situation and sectionalism. During the period of the revival of the learning from Youngjo till Jeongjo(正祖) the function of the library was in a stagnant state. Kyujanggak played the part in its place. 4. Referring to the collection management, the Munheongakseomog is equipped with 101 titles, 2,525 volumes, which are arranged by means of the traditional Chinese classification system. 5. The classification scheme is based on the traditional Chinese classification system which might divide all subjects into four categories such as: Confucian classics division, Historical documents division, Master's division, and Collection of literature division. Some illustrations reveal that the classification system was directly influenced by Seogoseomg (書庫書目) : the influence reflected in the classes for the translated literature and writings, poems, genealogy about kings, etc. But some subdivisions such as a class of Annals, Historical Epcerpts were omitted in the classification scheme, which did not strike the balance in the system in terms of the present theory of classification. Most of bibliographical descriptions were also influenced by Seogoseomog but some elements were partly omitted. 6. The special feature of the collection building is the absence of books in Collection of literature division except only three kinds of books in examining the Munheongakseomog. Since this is rather a comprehensive study for such aspects as historical backround, catalogue, and cultural environment of Munheongak and its related record, it is advised that further and additional research should be made.

• 공업화학
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• 제21권3호
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• pp.272-277
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• 2010

현재의 값비싼 광생물반응기를 대체하기 위하여 비닐백을 소재로 한 보급형 광생물반응기를 개발하고자 본 연구를 실시하였다. 앞선 연구에서 Spirulina platensis NIES 39의 최적배양조건을 확립하였으며, 이를 토대로 하여 이산화탄소 고정화 연구를 실시하였다. 이산화탄소 농도 및 유속에 따른 성장은 10% $CO_2$, 0.1 vvm의 조건에서 가장 높은 2.677 g/L의 건조균체량을 나타냈으며, 이산화탄소 고정화량($F_{CO_2}$)은 4.056 g ${\cdot}{CO_2}$/L, 이산화탄소 고정화속도($R_{CO_2}$)는 0.312 g $CO_2$/L/day로 나타났다. 반면, 이산화탄소 고정화 효율($FE_{CO_2}$)의 경우 5% $CO_2$, 0.1 vvm의 조건에서 나타난 데이터에 비해서 그 절반 수준인 52.372%를 나타내었다. 그리고 빛의 유무에 따른 이산화탄소 주입효과를 알아본 결과, 빛이 있는 조건에서 약 3 h 주기로 10 min간 주입하는 조건이 가장 우수한 성장 및 이산화탄소 고정화를 한 것으로 나타났으며, 빛이 없을 때는 이산화탄소 주입이 무의미하다는 결론을 얻을 수 있었다.

• Journal of Periodontal and Implant Science
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• 제32권4호
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• pp.733-744
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• 2002

The fibroblasts are the principal cells in the periodontal ligament of peridontium. As the periodontal ligament fibroblasts (PDLF) show similar phenotype with osteoblasts, the PDLF are thought to play an important role in alveolar bone remodeling. Cell-to-cell contacted signaling is crucial for osteoclast formation. Recently it has been reported that PDLJ enhance the bone resorbing activity of osteoclasts differentiated from hematopoietic preosteoclasts. The aims of this study were to $clarify\;^{1)}$ the mechanism of PDLF-induced osteoclastogenesis $and\;^{2)}$ whether we can use preosteoclast cell line instead of primary hematopoietic preosteoclast cells for studying the mechanism of PDLF-induced osteoclastogenesis. Osteoclastic differentiation of mouse macrophage cell line RAW264.7 was compared with that of mouse bone marrow-derived M-CSF dependent cell (MDBM), a well-known hematopoietic preosteoclast model, by examining, 1) osteoclast-specific gene expression such as calcitonin receptor, M-CSF receptor (c-fms), cathepsin K, receptoractivator nuclear factor kappa B (RANK) ,2) generation of TRAP(+) multinucleated cells (MNCs), and 3) generation of resorption pit on the $OAAS^{TM}$ plate. RAW264.7 cultured in the medium containing of soluble osteoclast differentiation Factor (sODF) showed similar phenotype with MDBM-derived osteoclasts, those are mRNA expression pattern of osteoclast-specific genes, TRAP(+) MNCs generation, and bone resorbing abivity. Formation of resorption pits by osteoclastic MNCs differentiated from sODF-treated RAW264.7, was completely blocked by the addition of osteoprotegerin (OPG), a soluble decoy receptor for ODF, to the sODF-containing culture me야um. The effects of PDLF on differentiation of RAW264.7 into　the TRAP(+) multinucleated osteoclast-like cells were examined using coculture system. PDLF were fxed with paraformaldehyde, followed by coculture with RAW264.7, which induced formation of TRAP(+) MNCs in the absence of additional treatment of sODF. When compared with untreated and fixed PDLF (fPDLF), IL-1 ${\beta}$-treated, or lipopolysaccha-ride-treated and then fixed PDLF showed two-folld increase in the supporting activity of osteoclastogenesis from RAW264.7 coculture system. There were no TRAP(+) MNCs formation in coculture system of RAW264.7 with PDLF of no fixation. These findigs suggested that we can replace the primary hematopoietic preosteoclasts for RAW264. 7 cell line for studying the mechanism of PDLF-induced osteoclastogenesis, and we hypothesize that PDLF control osteoclastogenesis through ODF expression which might be enhanced by inflammatory signals.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 추계학술대회 및 정기총회
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• pp.13-13
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• 2014

Species belonging to the genus Fusarium are widely distributed and cause diseases in many plants. Isolation of fungal strains from air or cereals is necessary for disease forecasting, disease diagnosis, and population genetics [1]. Previously we showed that Fusarium species are resistant to toxoflavin produced by the bacterial rice pathogen Burkholderia glumae while other fungal genera are sensitive to the toxin, resulting in the development of a selective medium for Fusarium species using toxoflavin [2]. In this study, we have tried to elucidate the resistant mechanism of F. graminearum against toxoflavin and interaction between the two pathogens in nature. To test whether B. glumae affects the development of F. graminearum, the wild-type F. graminearum strains were incubated with either the bacterial strain or supernatant of the bacterial culture. Both conditions increased the conidial production five times more than when the fungus was incubated alone. While co-incubation resulted in dramatic increase of conidial production, conidia germination delayed by either the bacterial strain or supernatant. These results suggest that certain factors produced by B. glumae induce conidial production and delay conidial germination in F. graminearum. To identify genes related to toxoflavin resistance in F. graminearum, we screened the transcriptional factor mutant library previously generated in F. graminearum [3] and identified one mutant that is sensitive to toxoflavin. We analyzed transcriptomes of the wild-type strain and the mutant strain under either absence or presence of toxoflavin through RNAseq. Expression level of total genes of 13,820 was measured by reads per kilobase per million mapped reads (RPKM). Under the criteria with more than two-fold changes, 1,440 genes were upregulated and 1,267 genes were down-regulated in wild-type strain than mutant strain in response to toxoflavin treatment. A comparison of gene expression profiling between the wild type and mutant through gene ontology analysis showed that genes related to metabolic process and oxidation-reduction process were highly enriched in the mutant strain. The data analyses will focus on elucidating the resistance mechanism of F. graminearum against toxoflavin and the interaction between the two pathogens in rice. Further evolutionary history will be traced through figuring out the gene function in populations and in other filamentous fungi.