• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.261-264
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• 2002

CHO (Chinese hamster ovary) cells were transfected with plasmids containing both cis-acting HRE (hypoxia response element) and CMV-promoter that controls tissue-type plasminogen activator (t-PA). CHO cells with HRE produced 16.2 fold higher t-PA concentration than CHO cells without HRE. It was noted that hypoxia strongly induced CHO cell apoptosis. which resulted in decrease of cell viability and protein production. In this study. by introducing Bcl-2, anti-apoptotic gene, we tried to recover cell viability and increase the protein production. When batch culture of both control cells without transfection of Bcl-2 and cells transfected with Bcl-2 were performed in the absence of CoCl ι hypoxia mimic condition. the cells with Bcl-2 were effected specific cell growth rates, maximum cell density. Immunoblotting assay showed Bcl-2 was recombinant with HRE dependent t- P A expression cassette, and their expression level was depended on hypoxia. By introducing Bcl-2, both cell viability and maximum cell density could be increased.

• Journal of the Korean Institute of Rural Architecture
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• v.5 no.2
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• pp.20-32
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• 2003

In this study, I grasped the actual conditions of folk exhibition hall in Chungbuk area. From this data I researched basically as the fundamental role of regional museum, eco-museum core center facilities, the connection with islanded and various inheritances, and central facilities whole area to the museum. And I could get following conclusion. First, on the studied result of folk exhibition hall in chungbuk area, it is not enough the activity of unearthing, collection, study etc. that is basic activity of the museum because the museum just permanently displayed historical contents of the area and simple life folk data. Second, add to existent museum concept and have to widen viewpoint to the museum which whole area is no roof, and utilize folk exhibition hall as the central facilities. Specially, there are a lot of difficulties to communicate information to visitors because of the absence of cultural heritance exponent. So a plan that local resident participate actively in museum activity may have to be considered. Third, the concept of inheritance limited in historical culture of area has to be widening by nature and industry etc. And we should keep up study that is the linked plan to scattered conservation remains, inheritance and tourist place in area.

• Korean Journal of Veterinary Research
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• v.47 no.2
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• pp.197-202
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• 2007

This experiment has been investigated in order to examine larval migration inhibition activity of ivermectin, doramectin and ethanol against Anisakis simplex (A. simplex) in vitro. A. simplex larvae were obtained from the mackerel acquired from the fish market of Cheongju. They were divided into many groups and placed in culture dishes (40 larvae each) containing RPMI-1640, in the absence or presence of different concentrations of ivermectin, doramectin and ethanol. Ivermectin had a complete inhibition of larval migration at 72 h in all groups ($10-300{\mu}g/ml$). Ethanol reduced the migration of the larvae, its maximum activity being an high doses (7.5%, 10% ethanol) when it was 100% efficacy at 4 h. Doramectin had no efficacy in vitro. Being needed that further studies with ivermectin and doramectin, it is recommended that in vivo test with laboratory animals should be carried.

• Archives of Plastic Surgery
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• v.40 no.5
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• pp.627-629
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• 2013

The ReCell system (Avita Medical) is a cell culture product that allows the immediate processing of a small split-thickness skin biopsy to produce a complete population of cells including keratinocytes, melanocytes, Langerhans cells and fibroblasts. This series is the first to highlight the reconstructive applications of ReCell following ablative skin cancer surgery. The ReCell system was utilized for three patients following skin cancer excision. In two cases, the cells were applied to forehead flap donor sites following nasal reconstruction. In one case, the cells were applied to the calvarial periosteum following wide local excision of a melanoma scar. Assessment of the treated area was performed using the patient and observer scar assessment scale after 1 year. The Patient and Observer Scar Assessment Scale (POSAS) scores for the 2 patients treated with ReCell following forehead flap surgery were 22 and 32. The score for the patient that underwent wide local excision of a melanoma scar was 45. The absence of a donor site, accelerated healing and the satisfactory aesthetic appearance of the mature scars in this series suggest that ReCell may play a useful role in reconstruction following skin cancer excision.

• Proceedings of the Korean Institute of Interior Design Conference
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• 2007.11a
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• pp.30-36
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• 2007

Recently, many performing arts facilities are under construction to accommodate people's various interest in cultural experiences. However, due to Korea's lack of know-how in such constructions and an absence of proper regulations on design planning, each designer designs and constructs the facilities differently, so several problems are occurring in the process of construction, such as high production cost for stage sets, high labor cost, low efficiency of stage work, and complicated work process. This resulted in low quality production of performance. This study is conducted to address the need for a systematic study on stage floor, and to propose an efficient way of regulating stage work in existing performing arts facilitiesand new facilities to be built. By a comparative analysis of performing arts facilities in Korea, and by analyzing stage floors of the facilities, this research suggests minimum modules as well as an appropriate unit of modules based on the minimum modules; and provides basic data on stage floors, which can be used for remodeling existing facilities orfor planning new cultural facilities. Also, this study suggests various ways of utilizing performing arts facilities in Korea.

• Environmental Engineering Research
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• v.15 no.1
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• pp.3-7
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• 2010

To better understand the ecology of tetrade forming organisms (TFOs) floating in a large amount of dairy wastewater treatment plant (WWTP) effluent (sequencing batch reactor [SBR]) during the inefficient phosphorus (P) removal process of an enhanced biological P removal system, the TFOs from the effluent of a full scale WWTP were separated and attempts made to culture the TFOs in presence/absence of oxygen. The intact TFOs only grew aerobically in the form of unicellular short-rods. Furthermore, to identify the intact TFOs and unicellular short-rods the DNAs of both were extracted, analyzed using their denaturing gradient gel electrophoresis (DGGE)-profiles and then sequenced. The TFOs and unicellular short-rods exhibited the same banding pattern in their DGGE-profiles, and those sequencing data resulted in their identification as Acinetobacter sp. The intact TFOs appeared in clumps and packages of tetrade cells, and were identified as Acinetobacter sp., which are known as strict aerobes and efficient P-removers. The thick layer of extracellular polymeric substance surrounding Acinetobacter sp. may inhibit phosphate uptake, and the cell morphology of TFOs might subsequently be connected with their survival strategy under the anaerobic regime of the SBR system.

• Journal of Information Science Theory and Practice
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• v.7 no.4
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• pp.6-19
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• 2019

The purpose of this article is to problematize the existence of a possible Ibero-American informational thinking. It was initially observed that a relative absence of Ibero-America in the international presentations and mappings of information science exists. Below, the reality of the 22 countries that compose Ibero-America is discussed, a region that can be understood from a sociocultural and geopolitical perspective. Then, a mapping of the information science research in these countries is made. The main research topics found are: epistemological studies, relationships with library science, information literacy, representation and organization, bibliometric studies, information management, user studies, technological dimensions, and relationships with archival science and museum studies. Finally, a general epistemological configuration of information science is presented at a global level, highlighting the great trends of study of information that marked the decades of the 1960s and 1970s (physical model), 1980s and 1990s (cognitive model), and the 21st century (sociocultural model), and which manifested themselves in the different subareas that make up the field. The most recent research in information science, in addition to addressing information transfer (physical dimension) and its relationship with data and knowledge (cognitive dimension), has also incorporated aspects related to the social effects of information, its role in the constitution of identities and culture, and the importance of its material conformations. Such expansion reflects attempts to address the complexity of informational phenomena. Therefore, it is concluded that it is important to place the specific contributions of Ibero-America in this context.

• Journal of Veterinary Clinics
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• v.37 no.4
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• pp.231-234
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• 2020

An 11-year-old, 4.3 kg, mixed breed, intact female dog exhibiting vomiting and a reduced appetite was presented. On physical examination, palpation of the right kidney elicited discomfort. Blood analysis revealed leukocytosis with neutrophilia, and elevation of alkaline phosphatase and blood urea nitrogen. Escherichia coli was detected in urine culture, whereas urine analysis showed absence of glucose. Radiography and ultrasonography suggested that there was gas in the right renal parenchyma, and computed tomography was performed while the dog was awake to investigate the suspected gas in the right kidney. After unilateral ureteronephrectomy, a histological diagnosis of right emphysematous pyelonephritis (EPN) was made. EPN refers to a severe necrotizing infection of the renal parenchyma with gas accumulation in the tissue. It is rare in both human and veterinary medicine, and almost all EPN patients have uncontrolled diabetes mellitus. This case report describes the imaging-based diagnosis of a rare occurrence of unilateral EPN in a non-diabetic dog.

• Cross-Cultural Studies
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• v.34
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• pp.71-92
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• 2014

This article presents a study on the concept of "Photogenie", which refers to the duality and mentality of the photographic image, with the viewpoint regarding the photographic image by Edgar Morin. First, we will look at the evolution of the concept of "Photogenie". From the field of photography, the term "Photogenie" means objects that produce light, enough to impress the photographic plate. But theorists of cinema at the beginning of the 20th century have changed the meaning of the term. For Louis Delluc, the "Photogenie" means the effect of union between the reproduction of real and artistic genius. For Jean Epstein, the "Photogenie" means mental quality, nonmaterial or inderterminable, of the photographic and cinematographic image. But Morin synthesized the arguements of Delluc and Epstein. For him, the "Photogenie" indicates both a double character of the photographic image and its mental quality. Then, based on this concept of "Photogenie," Morin said on particular aspects in the photographic image. Considering photography as a double in the anthropological sense, it puts emphasis not only the dual nature of the photographic image but also mental and spiritual quality. Combining the theory of the mage Henri Bergson and Jean-Paul Sartre, he builds his own theory of the mage that concerns both photography and cinema. In short, to Morin, the photographic image is a place where coexist absence and presence, the real and the imaginary, perception and memory, the material and mental, as well that a place of mentality which appear all our memories, hallucinations, dreams, imagination etc.

• Cross-Cultural Studies
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• v.39
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• pp.153-176
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• 2015

Poet, painter and publisher, Roland $Gigu{\grave{e}}re$ is one of Quebec's outstanding figures, inspired by both Surrealism and Quebec nationalism. He participated in contemporary artistic movement 'Phases' and influenced collective self-awareness and political ferment, 'Quiet Revolution'. In La Main au feu(1973), his poetry represent a landscape dominated by darkness in contrast with red color of fire from the volcanic crater. The world is immersed in darkness of despair which allude to the Great Darkness of Quebec society. Acts of violence assume many different forms: crows, black rain, dark flow, frenzy of knife blows. Both things and humans are in the state of absence or lack. Life falls into opacity of death. In the background of dark landscape, we discover Miror, a singular character. Similar to chain of mountains and to bare forest, he is a creature that shape the tragic inner world of poet. He is as like as seismograph that record the tremble of being. Finally, in order to fight the darkness of environment, the poet attempt to use the power of fire of volcanoes. The flow of magma become paintings of his dream and the flame of eruption, poetry of cry toward the sky. 'La main au feu' means the will to resist injustice and repression in the world. The tragic reality is replaced by a dream that become second reality out of reach of the force of hostile external circumstances.