• Korean Journal of Agricultural Science
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• v.22 no.1
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• pp.96-102
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• 1995

This study was conducted to investigate the effect of energy source on development of in vitro matured and fertilized porcine 2-cell embryos. The effects of glucose and phosphate for in vitro development of porcine 2-cell embryos were determined using modified BMOC-II medium. The results obtained are as follows. 1. In the absence of glucose and phosphate in the culture medium, 15.2% of porcine embryos developed 4-cell stage or further but morula formation was completely blocked. 2. The addition to 5mM glucose in the culture medium decreased rate of embryo reached 4-cell stage or further but increased morula stage as compared with addition to 0mM and 2.5mM glucose, regardless of the presence of phosphate. The addition to glucose in the culture medium without phosphate increased rate of embryo reached 4-cell stage or further and morula stage as compared with both addition to glucose and phosphate, regardless of added glucose concentration.

• International Journal of Advanced Culture Technology
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• v.9 no.3
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• pp.174-180
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• 2021

The necessity of this study is as follows. A decrease in the number of newborns, an increase in the youth unemployment rate, and a decrease in the employment rate are having a fatal impact on universities. To help increase the employment rate of universities, we intend to utilize Big Data of university public information. Big data refers to the process of collecting and analyzing data, and includes all business processes of finding data, reprocessing information in an easy-to-understand manner, and selling information to people and institutions. Big data technology can be divided into technologies for storing, refining, analyzing, and predicting big data. The purpose of this study is to find the vision and special department of a university with a high employment rate by using big data technology. As a result of the study, big data was collected from 227 universities on www.academyinfo.go.kr site, We selected 130 meaningful universities and selected 25 universities with high employment rates and 25 universities with low employment rates. In conclusion, the university with a high employment rate can first be said to have a student-centered vision and university specialization. The reason is that, for universities with a high employment rate, the vision was to foster talents and specialize, whereas for universities with a low employment rate, regional bases took precedence. Second, universities with a high employment rate have a high interest in specialized departments. This is because, as a result of checking the presence or absence of a characterization plan, universities with a high employment rate were twice as high (21/7). Third, universities with high employment rates promote social needs and characterization. This is because the characteristic departments of universities with high employment rates are in the order of future technology and nursing and health, while universities with low employment rates promoted school-centered specialization in future technology and culture, tourism and art. In summary, universities with high employment rates showed high interest in student-centered vision and development of special departments for social needs.

• Journal of Digital Convergence
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• v.17 no.8
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• pp.373-378
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• 2019

In various cultures and civilizations, artists are trying new ideas of reinterpreting the beauty of classic and redefining it in the ways of modern times rather than creating something new. In other words, modern cultural trend is creating a new culture by combining sensibilities of classic amid the flood of information with modern senses of artists. This study tries to combine various cultures an designs in accordance with this cultural change. Based on the features of expressing emotions of individuals and the world of impossible experiences from the short story 'When Buckwheat Flowers Bloom' of Lee Hyoseok, an incomplete area of consciousness which is at the border line of the polarized world divided by existence and absence is the area which creates a new culture by arousing readers' imagination as a potential world of thought. In addition, based on language from the short story, a researcher tries to utilize symbolic and imaginal beauties inside of that language as a material of design, and formative analysis them with the composition of proper words for visualization.

• Journal of Convergence for Information Technology
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• v.9 no.4
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• pp.8-13
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• 2019

In this paper, based on the production technology and infrastructure of local cultural contents, it is determined that reconstruction and commercialization of local culture resources are necessary due to absence of character brand products centered on storytelling. It is expected to contribute to the expansion of the supply of advanced ceramic products along with the foundation of various contents production as it becomes the foundation to establish the developed business base through the differentiation strategy of the character product development based on the storytelling. In addition, due to the manual production, only a small amount of production can be produced and the production period is lengthened. On the other hand, the production period of gypsum mold is shortened by making mock-up through 3D program and 3D printing, It was effective in developing a wide variety of pottery products.

• Journal of Microbiology and Biotechnology
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• v.31 no.12
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• pp.1624-1631
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• 2021

Prodigiosin as a high-valued compound, which is a microbial secondary metabolite, has the potential for antioxidant and anticancer effects. However, the large-scale production of functionally active Hahella chejuensis-derived prodigiosin by fermentation in a cost-effective manner has yet to be achieved. In the present study, we established carbon source-optimized medium conditions, as well as a procedure for producing prodigiosin by fermentation by culturing H. chejuensis using 10 L and 200 L bioreactors. Our results showed that prodigiosin productivity using 250 ml flasks was higher in the presence of glucose than other carbon sources, including mannose, sucrose, galactose, and fructose, and could be scaled up to 10 L and 200 L batches. Productivity in the glucose (2.5 g/l) culture while maintaining the medium at pH 6.89 during 10 days of cultivation in the 200 L bioreactor was measured and increased more than productivity in the basal culture medium in the absence of glucose. Prodigiosin production from 10 L and 200 L fermentation cultures of H. chejuensis was confirmed by high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) analyses for more accurate identification. Finally, the anticancer activity of crude extracted prodigiosin against human cancerous leukemia THP-1 cells was evaluated and confirmed at various concentrations. Conclusively, we demonstrate that culture conditions for H. chejuensis using a bioreactor with various parameters and ethanol-based extraction procedures were optimized to mass-produce the marine bacterium-derived high purity prodigiosin associated with anti-cancer activity.

• Journal of Microbiology and Biotechnology
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• v.33 no.11
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• pp.1437-1447
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• 2023

A recently bioinformatic analysis of genomic sequences of fungi indicated that fungi are able to produce more secondary metabolites than expected. Despite their potency, many biosynthetic pathways are silent in the absence of specific culture conditions or chemical cues. To access cryptic metabolism, 108 fungal strains isolated from various sites were cultured with or without Streptomyces sp. 13F051 which mainly produces trichostatin analogues, followed by comparison of metabolic profiles using LC-MS. Among the 108 fungal strains, 14 produced secondary metabolites that were not recognized or were scarcely produced in mono-cultivation. Of these two fungal strains, Myrmecridium schulzeri 15F098 and Scleroconidioma sphagnicola 15S058 produced four new compounds (1-4) along with a known compound (5), demonstrating that all four compounds were produced by physical interaction with Streptomyces sp. 13F051. Bioactivity evaluation indicated that compounds 3-5 impede migration of MDA-MB-231 breast cancer cells.

• Korean Journal of Animal Reproduction
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• v.23 no.2
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• pp.105-111
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• 1999

The effects of exogenous spleen cells on the progesterone and insulin like-growth factor-I (IGF-I) secretions in luteal cells were studied by using in vitro luteal cell culture system in the Hanwoo luteal cells. The corpora lutea(CL) were collected and pooled from the Korean native cattle(Hanwoo) ovaries from a local slaughter house. After enzymatic dissociation, combined large and small luteal cells(LLC and SLC)(1.0$\times$10$^{6}$ cells/$m\ell$) were incubated in D-MEM media containing antibiotics and 10% FCS. Spleen cells (1.0$\times$10$^{6}$ cells/$m\ell$) obtained from castrated adult male Hanwoo were added to luteal cells and co-cultured for 24 h in the absence or presence of luteinizing hormone (LH) (100 ng). Progesterone contents from luteal tissues were increased at CL-3 stage during each stage of estrous cycle. Progesterone secretion from luteal cell culture by the presence of LH (100 ng/$m\ell$) was positively stimulated compared with control. However, progesterone secretion was not changed by the addition of 5, 10 and 20% of spleen cells in the absence of LH. Co-culture of luteal cells with 10% of spleen cells in the presence of LH(l00ng/$m\ell$) significantly. enhanced after 24 h of culture. IGF-Isecretion from in vitro luteal cells co-culture by the addition of spleen cells (5%, 10% and 20%) was not significantly effected. Besides, in the presence of LH (100ng/$m\ell$), IGF-Isecretions from luteal cells by addition of spleen cells were higher than control media. However, LH alone significantly increased IGF-I secretion at 24 h of culture. These data provide the demonstrate that spleen cells can enhance LH action so as to stimulate progesterone secretion from Hanwoo luteal cells but have no effect to stimulate IGF-I secretion.

• Korean Journal of Human Ecology
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• v.12 no.3
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• pp.337-349
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• 2003

This study was dealt with consumers' attitudes and requests for refunds on the basis of data gathered in 1997 and 2000 and examined factors shaping the differences of the consumers' requesting for refunds and investigated variables influencing the presence or absence of requests for refunds. Research outcomes could be summarized as follows. First there were no differences in consumers' searches for information of refunds, degrees of satisfactions for practices of refunds, knowledges for refunds, consumers' expectation for the permission of refunds between two periods Consumers generally showed more active behaviors for the request for refunds in 2000 than in 1997. In cases of defects, the chance of getting refunds was higher while consumers feel greater needs for refund. Second, consumers perception for the necessity of refund and their expectations for successful outcomes were higher in cases of defects than in the opposite case; consumers were also more active to request for refunds and get more positive responses in the former case. Third, in cases of defects in 1997, as consumers faced the unsatisfactory response for their previous requests for refunds, they did not actively request for refunds. In 2000, requesting for refunds were more active as they were more knowledgeable, actively search for information of refunds before their purchases, and expect more positive outcomes for their requests for refund. On the other hand, in cases of no defects in 1997, consumers showed more active behaviors requesting for refunds as their own professional jobs, owned greater consumer expectation for positive outcomes and showed greater satisfactions for the previous requests of refunds. In the survey of 2000, consumers showed active behaviors requesting for refunds as they were highly educated, gather much information for refund, and showed positive expectations for the possibility of refund.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.114-114
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• 2003

Epidermal growth factor (EGF) induces well-documented mitogenic and differentiating effects on murine and bovine preimplantation embryos. However, the effects of EGF on apoptosis and implantation-related gene expression in bovine embryos developing in vitro have not been evaluated. The objective of this study was to determine the effects of exogenous EGF in the presence and absence of BSA on the preimplantation development of bovine embryos. In addition, we measured cell number, apoptosis, and expression of apoptosis and implantation-related genes of the blastocysts that developed in these culture conditions. In vitro produced bovine embryos were randomly cultured in the same medium containing 0 or 10 ng/ml EGF in the presence and absence of 0.8% BSA. More 2-cell embryos developed into blastocysts at day 7 when BSA was present than when BSA was absent. The addition of 10 ng/$m\ell$ EGF into the medium did not significantly increase the developmental rate and the cell numbers per blastocyst. However, addition of EGF in the presence of 0.8% BSA significantly reduced the degree of apoptosis in the blastocysts (P<0.01). To investigate whether EGF modulates mRNA expression of apoptosis-related genes, mRNA was prepared from single blastocysts and each preparation was subjected to RT-PCR for Bcl-2 and Bax transcripts. EGF did not alter the relative abundance of Bax gene expression in the presence of BSA, but increase Bcl-2 (P<0.01) The relative abundance of Interferon tau expression was increased by EGF treatment in the presence of BSA. These results suggest that EGF and BSA synergistically enhance Bcl-2 and interferone tau gene expression, which may result in a net increase in viability in bovine embryos.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.99-99
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• 2003

Epidermal growth factor (EGF) induces well-documented mitegenic and differentiating effects on murine and bovine preimplantation embryos. However, the effects of EGF on apoptosis and implantation-related gene expression in bovine embryos developing in vitro have not been evaluated. The objective of this study was to determine the effects of exogenous EGF in the presence and absence of BSA on the preimplantation development of bovine embryos. In addition, we measured cell number, apoptosis, and expression of apoptosis and implantation-related genes of the blastocysts that developed in these culture conditions. In vitro produced bovine embryos were randomly cultured in the same medium containing 0 or 10 ng/$m\ell$ EGF in the presence and absence of 0.8% BSA. More 2-cell embryos developed into blastocysts at day 7 when BSA was present than when BSA was absent. The addition of 10 ng/$m\ell$ EGF into the medium did not significantly increase the developmental rate and the cell numbers per blastocyst. However, addition of EGF in the presence of 0.8% BSA significantly reduced the degree of apoptosis in the blastocysts (P< 0.01). To investigate whether EGF modulates mRNA expression of apoptosis-related genes, mRNA was prepared from single blastocysts and each preparation was subjected to RT-PCR for Bcl-2 and Bax transcripts. EGF did not alter the relative abundance of Bax gene expression in the presence of BSA, but increase Bcl-2 (P < 0.01). The relative abundance of Interferon tau expression was increased by EGF treatment in the presence of BSA. These results suggest that EGF and BSA synergistically enhance Bcl-2 and interferone tau gene expression, which may result in a net increase in viability in bovine embryos.