• Fisheries and Aquatic Sciences
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• 제3권2호
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• pp.151-155
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• 2000

Phaeodactylum tricornutum (Bacillariophyceae) is a marine diatom which has been supplied as a food of bivalves. In this study, growth responses of P. tricornutum to some nitrogen sources and sodium were investigated by measuring cell number and contents of chlorophyll a in culture. In medium with nitrogen and sodium, brisk cell division occurred and maximum growth rate was respectively found in the medium with 150 mg/l of nitrate and 10 mg/l of ammonium and urea. At 10-500 mg/l ammonium and urea and 200-500 mg/l nitrate, specific growth rate decreased slightly. However, no cell division observed in sodium-deficient medium, regardless of presence or absence of nitrogen. This suggests that sodium is required for the nitrogen uptake of P. tricornutum, resulting nitrogen uptake leading to cell division. Also the upper limits of ammonium and nitrate for the growth of P. tricornutum seem to be 10 mg/l and 500 mg/l, respectively.

• 대한간호
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• 제30권4호
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• pp.61-68
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• 1991

This study was conducted to testing the criterion - related validity of a mental health assessment tool which developed based on a korean culture. Criteria scale for this tool were MMPI and CMI(M - R). The study subject were 100 male and female aged 20 or more with quota sampling. The data was collected from August 16. to August 26. 1989. The data obtained from 85 subjects were analysed using S.P.S.S.(Stastistical Package for the Social Science). As a result, there are no significant correlation between Mental Health Assessment Tool and MMPI and CMI except Mf(Masculinity-Feminity) Subscale of MMPI. This result means the MMPI and CMI was not related to tool which developed based on medical model from etic perspectives, although the tool which had been developed in America Modified to Korean situation. So I dare to say that only the absence of mental illness does not means mental health and the diagnosis of mental illness is not the only criteria of a mental health.

• 한국가축번식학회지
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• 제10권1호
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• pp.36-41
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• 1986

These experiments were carried out to obtain basic information necessary for sexing embryos by chromosomal analysis. To observe metaphase chromosomes, all embryos developed to blastocysts were cultured in Ho, pp. & Pitts' medium containing 0.001% Colcemid under the gas phase of 5% CO2 in air at 37$^{\circ}C$ for 2 hours. The sex chromosome of mouse embryos shown normal development after culture in medium containing H-Y antiserum (10%, v/v) and complement (20%, v/v) also was confimed by chromosomal analysis. The results obtained in these experiments were summarized as follows: 1. Among 89 mouse blastocysts, the number of embryos identified to have XX and XY chromosome was 22(25%) and 25(28%), respectively and 42(47%) embryos were not identified. 2. Of total 40 mouse balstocysts cultured in medium containing H-Y antiserum and complement, 23(58%) embryos which were able to be discriminated their sex chromosomes were identified to be XX bearing embryos. 3. Sex chromosomes of a number of embryos subjected to chromosomal analysis were not identified. This result may be due to absence or poor quality of metaphase spreads.

• 대한본초학회지
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• 제25권1호
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• pp.1-7
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• 2010

Objectives : We investigated genetic toxicity of HMCO5 in relation to chromosome aberration test on Cultured Chinese Hamster Lung (CHL) in the presence and absence of S-9 mix. Methods : Experimental groups were divided into two groups: with S-9mix (+S) or without S-9 mix (-S). -S group was also divided 2 series by treatment hours (6 hr: 6-S; or 24 hr; 24-S). Each group treated with vehicle only (complete culture medium), HMCO5 (1,250, 2,500, $5,000\;{\mu}g/ml$), and cyclophosphamide monohydrate (CPA) and ethylmethanesulfonate (EMS), respectively. Results : HMC05 did not show any aberrant metaphase. However, there were significant (p < 0.01) aberrant metaphases with CPA in S+ and with EMS in S-. Conclusions : These results indicate that HMC05 formula does not show any toxicity in chromosome aberration test.

• Archives of Pharmacal Research
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• 제17권3호
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• pp.199-203
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• 1994

Several lines evidence indicate that microtubule depolymerization initiates DNA synthesis or enhances the effects of serum or purified growth factors in many types of fibroblasts. Yet little is known about the intracellular events responsible for the mitogenic effect of microtubule disrupting agents. The effects of antitubulin agents on DNA synthesis in sparse and dense cultures in the presence or absence of serum and possible involvement of G-proteins in their mitotic action were examined. In these studies, colchicine by itself appeared to be mitogenic only for confluent quiesecent human lung fibroblasts. In sparse culture, however, colchicine inhibited serum-stimulated DNA synthesis. Colcemid, another antitubulin agent, showed similar effects of growth inhibition and stimulation in sparse and confluent cultures while lumicolhicine, inactive colchicine, did not. The mitogenic effect of two antitubulin agents, colchicine and colcemid, was partially inhibited by pertussis toxin. These data suggest that microtubular integrity is associated with the expression of either negative or positive control on DNA synthesis and mitogenic effect of antitubulin agents may be partially mediated by pertussis toxin-sensitive G protein.

• 한국식생활문화학회지
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• 제35권4호
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• pp.387-391
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• 2020

This study assessed the color, polyphenol content, and sensory characteristics of dressing containing varying concentrations (0, 3, 6 and 9%) of freeze-dried Natto. We determined that the absence of freeze-dried Natto (0%) imparted the highest additive value for both L and a, b. The polyphenol contents obtained were 0.63, 0.69, 0.74, and 0.82 mg/g for 0, 3, 6 and 9% of dressing containing freeze-dried Natto, respectively. The appearance, color, taste, texture, and overall preference of dressings with freeze-dried Natto showed significant differences between the samples. The overall preference was highest at 5.92 points, with dressing containing 9% Natto. The quality characteristics of the 9% added samples exhibited significantly higher values as compared to those of the controls, raising the possibility of development as a health-functional dressing.

• 한국콘텐츠학회논문지
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• 제14권5호
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• pp.411-424
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• 2014

본 연구의 목적은 서비스품질을 활용한 측정기준을 설정하여 지역문화축제에 대한 인식수준을 파악함과 동시에 서비스품질에 대한 중요도-성취도 분석을 통하여 향후 서비스품질 향상 및 방문객의 축제이용시 이용극대화를 위한 효율적인 개선운영방안을 모색하여 지역문화축제의 질적향상에 도움이 되고자 한다. 중요도와 성취도간 대응일치 t-test 결과 1)응답성 요인에서는 휴식공간여부, 시설배치도, 의사전달, 화장실비치/청결도 2) 확신성 요인에서는 음식가격, 종업원의 친절, 3)신뢰성 요인에서는 (행사장까지)축제팸플릿, 안내시설/표지판, 친절도, 스케줄안내, 신속한 대응 등 모든 항목, 4) 유형성 요인에서도 지역문화인지도, 유익성, 스토리텔링 등 모든 항목에서 5) 공감성 요인에서는 행사의 다양성과 축제내용 항목에서 유의한 차이가 있는 것으로 나타났다.

• 한국가축번식학회지
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• 제17권2호
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• pp.75-83
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• 1993

Purine has been identified in the preparation of follicular fluid and shown an activity in maintaining oocyte meiotic arrest. Therefore this study was performed to examine the inhibitory effect of purine on germinal vesicle break down(GVBD) in the presence and absence of human fetal cord serum(HFCS) or human mature follicular fluid(HMFF), as a protein source, in vitro culture. Immature oocytes(GV stage) were collected from ovaries of 21∼28 days old ICR mice by puncturing the antral follicles with a fine needle, at 48 hrs after PMSG injection. Some of the oocytes were denuded by drawing the cumulus-enclosed(complex) oocytes in and out of a pasteur pipet. Complex oocytes and denuded oocytes were cultured 3 hrs. in T6 media containing 0.75mM adenosine or/and 4mM hypoxanthine, with HFCS or HMFF. Their GVBD rates were observed at every 1 hr. during the culture time. Both adenosine and hypoxanthine have shown a time-dependent inhibitory effect on GVBD in complex and denuded oocytes and the inhibitory effect was maximized in culture medium containing hypoxanthine and adenosine. HFCS and HMFF increased the GVBD rates in the presence of the purines, thus HFCS and HMFF may contain a factor that could reverse the inhibitory effect of purines. Also complex oocytes were more sensitive to not only the inhibitory effect of purines but the promoting action of HMFF on GVBD than denuded oocytes. Therefore it was reconfirmed that granulosa cells play an important part in meiotic arrest and resumption.

• Strategy21
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• 통권42호
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• pp.293-317
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• 2017

A series of immoral conducts are repeatedly occurring by some commanders and high-ranking officers in the ROK navy. They are seriously compromising not only harmony and solidarity of the organization, but also pride and morale of members of the service. They seem to be results of the fragile ethical background of the service as well as of failures in military education and training on ethics. This paper sees backgrounds of weak ethical foundations of the navy as follows: the enduring legacies of cultural factors, such as the toxic military culture of the Japanese colonization, dark side of mariners' traditions, the sea-aversive Confucian culture, and unique characteristics in any small organization or society, etc... This paper also suggests the current ethnic systems of the navy are primarily based on responsive measures against misdeeds, rather than fundamental prescriptions for immoral behaviors, in the absence of ethical instrument for warriors. The paper also emphasizes the importance of developing character-based leadership, founded on, above all, respects for human integrity and strong sense of honor. Moreover, the importance of accountability in charging the command, as advocated by the US navy, is also stressed in order to establish a more robust ethic ground within the navy. Based on these analyses, the author proposes that in order to become a stronger and healthier fighting force, the navy needs to make continuous command efforts and organizational innovations to improve ethic principle and moral standard of the service, to strengthen ethics of naval leaders by a robust system of ethical education and training programs, and ultimately to solidify a navy culture in which moral passions and energies are spreading over.

• Journal of Acupuncture Research
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• 제22권2호
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• pp.55-70
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• 2005

Objective: Ulmus davidiana Planch (Ulmaceae) has long been known to have anti-inflammnatory in the traditional Korean medicine. UD has been reported as a good enhancer for bone healing. Methods : In this experiment, we investigate the Inhibitory effects of UD on bone resorption using the bone cells culture. Different concentrations of crude extract of UD were added to mouse bone cells culture. The mitochondria activity of the bone cells after exposure was determined by colorimetric MIT assay. It was demonstrated that UD has potential effects on bone cells culture without any cytotoxicity. The most effective concentration of UD on bone cells were $100\;{\mu}g/ml$. Cathepsin K (Cat K) is the major cysteine protease expressed in osteoclasts and is thought to play a key role in matrix degradation during bone resorption. Results : When mouse long bone cells including osteoclasts and osteoblast were treated with the PI3-Kinase inhibitor, wortmannin (WT), WT prevented the osteoclast-mediated intracellular processing of Cat K. Similarly, treatment of osteoclasts-containing long bone cells with UD extracts prevented the intracellular maturation of Cat K, suggesting that UD may disrupt the intracellular trafficking of pro Cat K. This is similar to that of WT. Since secreted proenzymes have the potential to reenter the cell via mannose-6-phosphate (M6P) receptor, to prevent this possibility, we tested WT and UD in the absence or presence of M6P. Inhibition of Cat K processing by WT or UD was observed in a dose-dependent manner. Furthermore, the addition of M6P resulted in enhanced potency of WT and UD. Conclusion : UD dose-dependently inhibited in vitro bone resorption with a potency similar to that observed for inhibition of Cat K processing.