• Title/Summary/Keyword: Abscisic acid

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A Micro-quantification of Abscisic Acid from Plant Tissue by Pentafluorobenzyl Esterification Using GLC (Pentafluorobenzyl ester 화(化)에 의(依)한 미량(微量) Abscisic Acid 의 GLC 분석법(分析法))

  • Jeong, Young-Ho;Hong, Moo-Ki;Song, Byung-Hun
    • Korean Journal of Environmental Agriculture
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    • v.4 no.2
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    • pp.114-117
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    • 1985
  • A new method for micro-quantification of abscisic acid from plant tissue is described. GLC-ECD method of abscisic acid pertafluorobenzyl ester synthesized by reaction of plant extract with pentafluorobenzyl bromide showed higher sensitivity than that of abscisic acid methyl ester, and the detection limit of abscisic acid by the described method was as low as 5 pg.

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The Stereospecific Synthesis of Abscisic Acid

  • Park, Oee-Sook;Lee, W.Y.;Park, J.C.
    • Korean Journal of Pharmacognosy
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    • v.17 no.1
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    • pp.67-72
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    • 1986
  • A stereospecific synthesis of 3-methyl-5-(1-hydroxy-4-oxo-2,6,6-trimethyl-2-cyclohexen-1-yl)-cis, trans-2,4-pentadienoic acid (abscisic acid) from ${\alpha}-ionone$ has been investigated. Ethyl 5-(2,6,6-trimetyl-2-cyclohexen-1-yl)-trans-4-penten-2-ynoate $({\alpha},{\beta}-acetylenic\;ester)$, which was synthesized from alpha-ionone in two steps, was stereospecifically converted in good yield into ethyl 3-methyl-5-(2,6,6-trimethyl-2-cyclohexen-1-yl)-cis, trans-2, 4-pentadienoate $({\alpha}-ionylideneacetate)$ by the conjugate addition of lithium dimethylcuprate at $-78^{\circ}C$. Basic hydrolysis of the ethyl ${\alpha}-ionylideneacetate$ gave an abscisic acid precursor, 3-methyl-5-(2,6,6-trimethyl-2-cyclohexen-1-yl)-cis, trans-2,4-pentadienoic acid, which can be oxidized to yield abscisic acid.

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Effects of $GA_3$ and ABA on Endogenous Starch Content during Shoot Differentiation in Cymbidium spp. Protocorm (Cymbidium spp.의 Protocorm 묘조 분화시 Protocorm내 전분 함량에 미치는 $GA_3$와 ABA의 영향)

  • 한태진
    • Journal of Plant Biology
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    • v.31 no.4
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    • pp.249-258
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    • 1988
  • Changes in starch content and activities of ADPG- and UDPG-starch synthase and $\alpha$- and, $\beta$-amylase were studied in order to investigate effects of gibberellic aicd and abscisic acid on endogenous starch content during shoot differentiation and protocorm propagation in Cymbidium spp. (Jungfrau) protocorm. Shoot differentiation was promoted during the degradation of endogenous starch and protocorn propagation was promoted during starch accumulation in protocorm. The activities of ADPG- and, UDPG-starch synthase and $\alpha$- and $\beta$-amylase seemed to be related with starch content. Shoot differentiation and protocorm propagation were slightly inhibited in protocorm explants treated with 100$\mu$M gibberellic acid. The explants treated with 10$\mu$M abscisic acid lost the capacity for shoot differentiation and protocorm propagation, and that could not be overcome by 100$\mu$M gibberellic acid added to culture medium. Starch content fluctuated as the control even after 10$\mu$M abscisic acid. None the less, the treatment completely inhibited shoot differentiation and protocorm propagation.

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Effects of Water Stress on Nitrate Reductase Activity and Abscisic Acid Content in Soybean Plants (수분부족이 콩의 질산환원효소 활성과 Abscisic acid 함량에 미치는 영향)

  • Ryu, Jeom-Ho;Doo, Hong-Soo;Ko, Gyong-Nam;Choo, Byong-Gil;Lee, Kang-Soo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.3
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    • pp.223-231
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    • 1994
  • To investigate effects of water stress on leaf water potential, nitrate reductase activity and abscisic acid content, Paldalkong, Saealkong and Danyeobkong were subjected to water stress by polyethyene glycol(PEG, MW=6,000) in the water culture and withholding irrigation in soil culture. Leaf water potential and nitrate reductase activity decreased with increaseing of PEG concentration in the water culture. These were higher at 3rd and 5th leaf stage than at 1st leaf stage. Leaf water potential showed no significant differance among the varieties, but nitrate redutase activity was higher in Paldalkong than in Saealkong and Danyeobkong. Leaf water potential and nitrate reductase activity decreased approximately 2.2 times (-1.67 MPa/-0.75MPa) and 47%(3.1${\mu}$ mole nitrite/g.DW/hour/15.9${\mu}$ mole nitrite/g.DW/hour) to control, respectively, after 3 days from water stress treatment in the soil culture. According to increasing PEG concentration, abscisic acid content increased in the water culture and was higher at 5th leaf stage than at 1st and 3rd leaf stage. Paldalkong showed the highest abscisic acid content. Abscisic acid content increased approximately 1.7 times (9.9nmole/g.DW/5.7nmole/g.DW) compared to the control after 3 days from water stress treatment in the soil culture.

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Growth and Abscisic Acid Changes of Creeping Thyme in the Exposure of NaCl and Drought (염 및 건조스트레스 하에서 포복형 백리향의 생육과 Abscisic Acid 농도변화)

  • Kim, Min-Jea;Eom, Seok-Hyun
    • Korean Journal of Medicinal Crop Science
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    • v.17 no.5
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    • pp.328-334
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    • 2009
  • Experimental purpose was to evaluate growth characteristic and abscisic acid (ABA) responses against salt/drought stresses. In the shoot biomass, creeping thyme was tolerated in mild NaCl stress, ranging 0 to 100 mM, while it was severely reduced in higher salinity. Under constant drought stress, the shoot biomass of creeping thyme showed a worse value compared to that of 100 mM NaCl treatment. Chlorophyll degradation was more severe in immature leaf than mature leaf under salt and drought stresses. In salt stress, immature leaf produced much amounts of ABA compared to mature leaf and also immature leaf showed faster increase of ABA than that of mature leaf. In drought stress, immature leaf responded to stress within 24 hours by the increase of ABA, while mature leaf responded to at 72 hours. Our results recommended that the optimal salinity level of creeping thyme was 50~100 mM NaCl.

Future Prospects for Industrial Application of Abscisic acid, a Stress-resistant Phytohormone (스트레스 내성 식물 호르몬인 앱시스산의 산업적 활용 전망)

  • Lee, Jeongho;Kim, Seunghee;Yoo, Hah Young
    • Korean Chemical Engineering Research
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    • v.58 no.4
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    • pp.514-523
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    • 2020
  • Plants are exposed to various types of stresses in their surroundings, and stress-resistant and regulatory proteins are produced as defense mechanisms. Abscisic acid is well known for its important role in stress signals as a phytohormone and is also involved in the physiological reactions of plants such as leaf senescence and seed dormancy. In particular, it has been found to perform a variety of functions in other biological systems, such as animals and microalgae, not plants. In this review, the biosynthesis and signaling process of abscisic acid and its function were investigated and the future prospects for the industrial application of abscisic acid in various biotechnologies, including agriculture, biomedical and industrial biotechnology, have been proposed based on study of emerging applications such as increased crop yields, disease treatment development and bioenergy production.

The Effect of Gibbrellic Acid and Abscisic Acid on Ribonucleic Acid and Ribonuclease in Barley Coleoptiles (Gibbrellic Acid와 Abscisic Acid가 보리 초엽(?葉)의 핵산(核酸) 및 핵산분해효소(核酸分解酵素)에 미치는 영향(影響))

  • Suh, Yong-Taek
    • Applied Biological Chemistry
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    • v.20 no.2
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    • pp.242-246
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    • 1977
  • In the barly coleptile sections treated with either $1{\times}10^{-5}M$ abscisic acid (ABA) or $1{\times}10^{-5}M$ gibberellic acid (GA), the time course changes of ribonuclease (RNase) activity and ribonucleic acid (RNA) profiles were studied. The results may be summarized as follows: 1. While GA suppressed RNase activity, ABA activated it. 2. High level of s-RNA and low level of r-RNA compared with normal plant sections in hormone-untreated coleoptiles seemed to be the results of increased RNase activity in the incubation period. 3. While GA retarded the decomposition of r-RNA, ABA activated it and the results seemed to be related with RNase activity. 4. GA activated the synthesis of RNA-DNA component, and ABA suppressed it. 5. Increase in the amount of s-RNA with the treatment of ABA may be due to the decomposition of r-RNA.

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