• 해양환경안전학회지
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• 제23권3호
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• pp.273-278
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• 2017

This paper is to study a technique to detect the real-time route aberrance on the passage route using bumper area of the ship domain theory. In order to evaluate the risk of route aberrance, a quarter line was created between the center line and the outer line, and a passage route with the image line outside the outer line was designed. It calculated the real-time route aberrance with the vessel bumper area to measure the risk level on the passage route. The route aberrance using overlap bumper area was simulated through three kinds of scenario vessel at the designed passage route. In this paper, we proposed Ratio to Aberrance Risk as one of the evaluation parameter to detect the route aberrance risk at each sector in the passage route and to give the evaluation criteria of 5 levels for seafarer's navigation safety. The purpose of this work is to provide the information of the route aberrance to seafarer automatically, to make it possible to prevent the human errors of seafarer on the high risk aberrance route. As the real-time risk of route aberrance on the passage route is automatically evaluated, it was well thought that seafarer can have only a little workload in order to know the risk of route aberrance at early-time. Following the further development of this work, the techniques for detecting the real-time route aberrance will be able to use the unmanned vessel.

• Asian Pacific Journal of Cancer Prevention
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• 제13권5호
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• pp.1943-1948
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• 2012

Introduction: Prostate cancer in Indonesia is the $3^{rd}$ ranking cancer among males and the $5^{th}$ rank for their cancer mortality. Prognostic markers that can identify aggressive prostate cancer in early stages and help select appropriate therapy to finally reduce the mortality are therefore urgently needed. It has been suggested that stem cells in the prostate gland have a role in initiation, progression, and metastasis of cancer, although controversy continues to exist. Maintenance of normal stem cell or reserve cell populations in several epithelia including prostate has been shown to be regulated by p63 and alteration of p63 expression is considered to have an oncogenic role in prostate cancer. We hypothesize that the expression of cytoplasmic aberrance of p63 is associated with high ALDH1A1 expression as a cancer stem cell marker, thus leading to progression of prostate cancer. Methods: Using a cross-sectional study during two years (2009-2010), a total of 79 paraffin embedded tissues of benign prostatic hyperplasia, PIN prostatic intraepithelial neoplasia, low and high Gleason score prostate cancer were investigated using immunohistochemistry. Associations between cytoplasmic p63 and ALDH1A1, as well as with pathological diagnosis, were analyzed by Chi-Square test using SPSS 15.0. Links of both markers with cell proliferation rate (KI-67) and apoptotic rate (cleaved caspase 3) were also analyzed by Kruskal-Wallis test. Results: The mean age of patient at the diagnosis is 70.0 years. Cytoplasmic aberrance of p63 was associated with ALDH1A1 expression (p<0.001) and both were found to have significant relationships with pathological diagnosis (including Gleason score), (p=0.006 and p<0.001 respectively). Moreover, it was also found that higher levels of cytoplasmic p63 were significantly associated with the frequency of proliferating cells and cells undergoing apoptosis in prostate cancers (p=0.001 and p=0.016 respectively). Conclusion: p63 cytoplasmic aberrance is associated with high ALDH1A1 expression. These components are suggested to have an important role in prostate cancer progression and may be used as molecular markers.

• 한국실내디자인학회논문집
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• 제23권1호
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• pp.96-104
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• 2014

The purpose of this study is to investigate Seonyudo Park and Kkummaru in terms of haptic space. This is also an attempt to explore temporality of appearing in architectural spaces with reference to cinematic expression in which time is visible and perceptible. The film of Chris Marker's La Jet$\acute{e}$e depicts temporal relationship by indeterminate continuum, reading the space on the disjunctive relationships of visual and auditory, and aberrance of time and space. Based on the three categories that is derived from those discussions, this study analyzes the experience of non-chronological time induced by architectural devices: the bifurcation of indeterminate circulation, the readability of space on the disjunction of visual and auditory, and the border-dismantling. Therefore, this study have a relationship with the contemporary discourse on time and events as transformation and becoming, and it means to escape from the deterministic thinking to emphasize invariability and space rather than variation and time.

• Animal Bioscience
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• 제35권11호
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• pp.1787-1799
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• 2022

Objective: Choline deficiency, one main trigger for nonalcoholic fatty liver disease (NAFLD), is closely related to lipid metabolism disorder. Previous study in a choline-deficient model has largely focused on gene expression rather than gene structure, especially sparse are studies regarding to alternative splicing (AS). In modern life science research, primary hepatocytes culture technology facilitates such studies, which can accurately imitate liver activity in vitro and show unique superiority. Whereas limitations to traditional hepatocytes culture technology exist in terms of efficiency and operability. This study pursued an optimization culture method for duck primary hepatocytes to explore AS in choline-deficient model. Methods: We performed an optimization culture method for duck primary hepatocytes with multi-step digestion procedure from Pekin duck embryos. Subsequently a NAFLD model was constructed with choline-free medium. RNA-seq and further analysis by rMATS were performed to identify AS events alterations in choline-deficency duck primary hepatocytes. Results: The results showed E13 (embryonic day 13) to E15 is suitable to obtain hepatocytes, and the viability reached over 95% by trypan blue exclusion assay. Primary hepatocyte retained their biological function as well identified by Periodic Acid-Schiff staining method and Glucose-6-phosphate dehydrogenase activity assay, respectively. Meanwhile, genes of alb and afp and specific protein of albumin were detected to verify cultured hepatocytes. Immunofluorescence was used to evaluate purity of hepatocytes, presenting up to 90%. On this base, choline-deficient model was constructed and displayed significantly increase of intracellular triglyceride and cholesterol as reported previously. Intriguingly, our data suggested that AS events in choline-deficient model were implicated in pivotal biological processes as an aberrant transcriptional regulator, of which 16 genes were involved in lipid metabolism and highly enriched in glycerophospholipid metabolism. Conclusion: An effective and rapid protocol for obtaining duck primary hepatocytes was established, by which our findings manifested choline deficiency could induce the accumulation of lipid and result in aberrant AS events in hepatocytes, providing a novel insight into various AS in the metabolism role of choline.