• Title/Summary/Keyword: ATFC

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Isolation and Characterization of a Novel Transcription Factor ATFC Activated by ER Stress from Bombyx mori Bm5 Cell Lines (누에 배양세포(Bm5)로부터 분리한 새로운 전사제어인자 ATFC의 특성분석)

  • 구태원;윤은영;김성완;최광호;황재삼;박수정;권오유;강석우
    • Journal of Life Science
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    • v.13 no.5
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    • pp.596-603
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    • 2003

  • Cells respond to an accumulation of unfolded proteins in the endoplasmic reticulum (ER) by increasing transcription of genes encoding molecular chaperones and folding enzymes. The information is transmitted from the ER lumen to the nucleus by intracellular signaling pathway, called the unfolded protein response (UPR). To obtain genes related to UPR from B. mori, the cDNA library was constructed with mRNA isolated from Bm5 cell lines in which N-glycosylation was inhibited by tunicamycin treatment. From the cDNA library, we selected 40 clones that differentially expressed when cells were treated with tunicamycin. Among these clones, we have isolated ATFC gene showing similarity with Hac1p, encoding a bZIP transcription factor of 5. cerevisiae. Basic-leucine zipper (bZIP) domain in amino acid sequences of ATFC shared homology with yeast Hac1p. Also, ATFC is up-regulated by accumulation of unfolded proteins in the ER through the treatment of ER stress drugs. Therefore we suggest that ATFC represents a major component of the putative transcription factor responsible for the UPR leading to the induction of ER-localized stress proteins.

  • https://doi.org/10.5352/JLS.2003.13.5.596 인용 PDF KSCI

A Bombyx mori Transcription Factor, ATFC Binds Directly to the UPRE of Molecular Chaperones

  • Goo, Tae-Won;Yun, Eun-Young;Kim, Sung-Wan;Park, Kwang-Ho;Hwang, Jae-Sam;Kwon, O-Yu;Kang, Seok-Woo
    • International Journal of Industrial Entomology and Biomaterials
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    • v.7 no.2
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    • pp.133-137
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    • 2003

  • Cells respond to an accumulation of unfolded proteins in the endoplasmic reticulum (ER) by increasing transcription of genes encoding molecular chaperones and folding enzymes. The information is transmitted from the ER lumen to the nucleus by intracellular signaling pathway, called the unfolded protein response (UPR). In Saccharomyces cerevisiae, such induction is mediated by the cis-acting unfolded response element (UPRE) which has been thought to be recognized by Hac1p transcription factor. We cloned the ATFC gene showing similarity with Hac1p, and then examined to determine whether ATFC gene product specifically binds to UPRE by electrophoretic mobility shift assays. ATFC gene product displayed appreciable binding ${to ^{32}}P-labelled$ UPRE. Therefore, we concluded that ATFC represents a major component of the putative transcription factor responsible for the UPR leading to the induction of ER-localized stress proteins.

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Bombyx mori Transcription Factor, ATFC Binds directly to the UPRE of Molecular Chaperones

  • Goo, Tae-Won;Yun, Eun-Young;Kim, Sung-Wan;Park, Kwang-Ho;Hwang, Jae-Sam;Kwon, O-Yu;Kang, Seok-Woo
    • Proceedings of the Korean Society of Sericultural Science Conference
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    • 2003.10a
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    • pp.76-76
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    • 2003

  • We describe here the identification of a transcription factor, ATFC that regulates the UPR by binding to the UPRE only when the signaling pathway is activated. The data in this study cover the first set of results, showing that ATFC has a major role in the insect UPR. (omitted)

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