• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.6
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• pp.1368-1378
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• 2009

Unfolded protein response (UPR) is an important genomic response to endoplasmic reticulum (ER) stress. The ER response is characterized by changes in specific proteins, induction of ER chaperones and degradation of misfolded proteins. Also, the pathogenesis of several diseases like Alzheimer's disease, neuronal degenerative diseases, and diabetes reveal the role of ER stress as one of the causative mechanisms. Borneolum has been used for neuronal disease in oriental medicine. In the present study, the protective effect of borneolum on thapsigargin-induced apoptosis in rat C6 glial cells. Treatment with C6 glial cells with 5 uM thapsigargin caused the loss of cell viability, and morphological change, which was associated with the elevation of intracellular $Ca^{++}$ level, the increase in Grp78 and CHOP and cleavage of pro-caspase 12 Furthermore, thapsigargin induced Grp98, XBP1, and ATF4 protein expression in C6 glial cells. Borneolum reduced thapsigargin-induced apoptosis through ER pathways. In the ER pathway, borneolum attenuated thapsigargin-induced elevations in Grp78, CHOP, ATF4, and XBP1 as well as reductions in pro-caspase 12 levels. Also, our data showed that borneolum protected thapsigargin-induced cytotoxicity in astrocytes from rat (P3) brain. Taken together, our data suggest that borneolum is neuroprotective against thapsigargin-induced ER stress in C6 glial cells and astrocytes. Accordingly, borneolum may be therapeutically useful for the treatment of thapsigargin-induced apoptosis in central nervous system.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.7 no.8
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• pp.1591-1597
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• 2003

In this paper, Active Resonator Oscillator using active inductor and active capacitor with HEMTs(agilent ATF­34143) is designed and fabricated. Active inductor with ­25$\Omega$ and 2.4nH in 5.5GHz frequency band and Active capacitor with ­14$\Omega$ and 0.35pF is designed. Active Resonator Oscillator for LO in ISM band(5.8GHz) is designed with active inductor and active capacitor. Active Resonator Oscillator has been simulated by Agilent ADS 2002C. Active Resonator oscillator implemented on the substrate which has the relative dielectric constant of 3.38, the height of 0.508mm, and metal thickness of 0.018mm. This Active Resonator Oscillator shows the oscillation frequency of 5.68GHz with the output power of ­3.6㏈m and phase noise of ­81㏈c/Hz at the offset frequency of 100KHz.

• Journal of Nutrition and Health
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• v.45 no.5
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• pp.420-428
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• 2012

The purpose of this study is to investigate the effects of eight weeks high fat intake and regular exercise in skeletal muscle and adipose tissue for Endoplasmic Reticulum (ER) stress in rats. This experiment involved 32 subjects (sprague-dawley rats) divided into four groups as follows: chow group (Chow, n = 8), chow and exercise group (Chow + EX, n = 8), high fat diet-induced hyperlipidemia group (HF, n = 8), and HF and exercise group (HF + EX, n = 8). As a result, there were significant decrease in body weight and abdominal fat, and blood lipid level was significantly improved by exercise for eight weeks (p < .05). There were variables changed about the skeletal muscle and ER stress in GRP78, XBP-1, ATF4, CHOP and JNK mRNA. There increased in mRNA factor by exercise, especially GRP78, and ATF4 mRNA were significantly increased in exercise (p < .05). However, there were increased in adipose tissue by exercise and there were significantly decreased in mRNA factor by high fat diet (p < .05). Consequently, this study suggests that the consistent exercise was more improved of obesity factor, such as dyslipidemia, hyperlipidemia, hyperglycemia, as well as body weight or abdominal fat. The response of ER stress in adipose tissue and skeletal muscle were more sensitive in exercise than high fat diet feed.

• Tribology and Lubricants
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• v.35 no.4
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• pp.229-236
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• 2019

This study investigates the stress singularity that occurs at the contact edge of three bodies in a frictional complete contact. We use the asymptotic analysis method, wherein we constitute an eigenvalue problem and observe the eigenvalue behavior, which we use to obtain the order of the stress singularity. For the present geometry of three bodies in contact, a contact between a cracked indenter and half plane is considered. This is a typical geometry of the PCMI problem of a nuclear fuel rod. Thus, this paper, specifically presents the characteristics of the PCMI problem from the perspective of stress singularity. Consequently, it is noted that the behavior of the stress singularity varies with the difference in the crack angle, coefficient of friction, and material dissimilarity, as is observed in a frictional complete contact of two bodies. In addition, we find that the stress singularity changes essentially linearly with respect to the coefficient of friction, regardless of the variation in the crack angle and material dissimilarity. Concurrently, we find the order of singularity to be 0.5 at a certain coefficient of friction, irrespective of the crack angle, which we also observe in the crack problem of a homogeneous and isotropic body. The order of singularity can also exceed 0.5 in the frictional complete contact problem of three bodies. This implies that the propensity for failure when three bodies are in frictional complete contact can be even worse than that in case of a failure induced by a crack.

• Explosives and Blasting
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• v.38 no.4
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• pp.26-36
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• 2020

With the increase of expansion and use of the underground space, the possibility of an underground explosion by terrorists is increasing. In this study, after modeling a circular tunnel excavated at a depth of 50m, an explosion load was applied to the inside of the tunnel. As for the explosion load, the explosion load of the maximum explosive amount for six types of vehicle booms proposed by ATF (Bureau of Alcohol, Tobacco, and Firearms) was calculated. For the rock mass around the circular tunnel, three types of rock grades were selected according to the support pattern suggested in the domestic tunnel design. Nonlinear dynamic analysis was performed to evaluate the influence of the ground structure by examining the surface displacement using the explosion load and rock mass characteristics as parameters. As a result of the analysis, for grade 1 rock, the influence on the uplift of the surface should be considered, and for grade 2 and 3 rocks, the influence on a differential settlement should be considered. In particular, for grade 3 rocks, detailed analysis is required for ground-structure interaction within 40m. Also, it is considered that the influence of Young's modulus is the main factor for the surface displacement.

• BMB Reports
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• v.32 no.4
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• pp.379-384
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• 1999

Taxol, a natural product with significant anti-tumor activity, stabilizes microtubules and arrests cells in the G2/M phase of the cell cycle. It has been reported that taxol has additional effects on the cell such as an increase in tyrosine phosphorylation of proteins and activation of mitogen-activated protein (MAP) kinase. This phosphorylated kinase translocates into the nucleus and phosphorylates its substrate c-jun, c-fos, ATF2, and ATF3. The MAP kinase family is comprised of key regulatory proteins that control the cellular response to both proliferation and stress signals. First examination was cytotoxicity and apoptosis-induced concentration with paclitaxel in HeLa cell. A half-maximal inhibition of cell proliferation ($IC_{50}$) occurred at 13 nM paclitaxel. When DNA fragmentation was analyzed by agarose gel electrophoresis, a nucleosomal ladder became evident 24 h after a taxol (50 nM) addition to the cells. In addition, an apoptotic body was detected by electron microscopy. Taxol-treated cells were arrested at the S phase at 10 nM. Treatment of 50 nM taxol activated the extracellular signal-regulated protein kinase (ERK1), and a fraction of the activated MAP kinases entered the nucleus. It was also discovered that nucleus substrates c-jun was phosphorylated and activated in the cell. The activated ERK1 could subsequently translocate into the nucleus and phosphorylate its substrate c-jun as well. This study suggests that taxol-induced apoptosis might be related with signal transduction via MAP kinases.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.2 no.4
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• pp.635-641
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• 1998

This paper presents development of a small size LNA operating at 824 ∼ 849 MHz used for a receiver of a CELLULAR CDMA Base station and a transponder. Using resistive decoupling circuits, a signal at low frequency is dissipated by a resistor. This design method increases the stability of the LNA and is suitable for input stage matching. The LNA consists of low noise GaAs FET ATF-10136 and internally matched VNA-25. The LNA is fabricated with both the RF circuit and the self-bias circuits in aluminum housing. As a result, the characteristics of the LNA implemented here shows above 35dB in gain and below 0.9dB in noise figure, 18.6dBm P1dB power, a typical two tone IM3, -31.17dB with single carrier backed off 10dB from P1dB.

• Molecules and Cells
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• v.47 no.1
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• pp.100004.1-100004.11
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• 2024

Insulin is essential for maintaining normoglycemia and is predominantly secreted in response to glucose stimulation by β-cells. Incretin hormones, such as glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide, also stimulate insulin secretion. However, as obesity and type 2 diabetes worsen, glucose-dependent insulinotropic polypeptide loses its insulinotropic efficacy, whereas GLP-1 receptor (GLP-1R) agonists continue to be effective owing to its signaling switch from Gs to Gq. Herein, we demonstrated that endoplasmic reticulum (ER) stress induced a transition from Gs to Gq in GLP-1R signaling in mouse islets. Intriguingly, chemical chaperones known to alleviate ER stress, such as 4-PBA and TUDCA, enforced GLP-1R's Gq utilization rather than reversing GLP-1R's signaling switch induced by ER stress or obese and diabetic conditions. In addition, the activation of X-box binding protein 1 (XBP1) or activating transcription factor 6 (ATF6), 2 key ER stress-associated signaling (unfolded protein response) factors, promoted Gs utilization in GLP-1R signaling, whereas Gq employment by ER stress was unaffected by XBP1 or ATF6 activation. Our study revealed that ER stress and its associated signaling events alter GLP-1R's signaling, which can be used in type 2 diabetes treatment.

• Proceedings of the Korea Electromagnetic Engineering Society Conference
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• 2001.11a
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• pp.252-256
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• 2001

본 논문에서는 IMT-2000 기지국용 2단 저잡음 증폭기를 설계했다. 잡음지수 특성이 뛰어난 HP사의 PHEMT 소자인 ATF-35143을 사용하였고 능동소자의 바이어스는 $V_{ds}$ 가 3 v $I_{d}$을 30mA로 설정했다. 첫 단은 최소잡음 지수에 중점을 두고 설계했고 둘째 단은 이득에 초점을 맞추어 설계했다. 입출력 정재파 비를 줄이기 위해서 전체증폭기의 앞단과 뒷단에 삽입손실이 0.2dB인 X503 SMT 90도 하이브리드 커플러를 설치 했다. 제작을 위해 기판은 두께 0.76mm이고 비유전율 4.2의 FR-4를 사용했다. 설계된 저잡음 증폭기의 특성은 주파수대역 1.92GHz～1.98GHz에서 잡음지수 0.45dB, 입출력 정재파 비 1.2이하, 이득은 32dB이상의 특성을 보였다.

• Structural Engineering and Mechanics
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• v.33 no.4
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• pp.391-406
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• 2009

Viscoelastic materials store as well as dissipate energy to the thermal domain under deformation. Two efficient modelling techniques reported in literature use coupled (thermo-mechanical) ATF (Augmenting Thermodynamic Fields) displacements and ADF (Anelastic Displacement Fields) displacements, to represent the constitutive relationship in time domain by using certain viscoelastic parameters. Viscoelastic parameters are first extracted from the storage modulus and loss factor normally reported in hand books with the help of Genetic Algorithm and then constitutive relationships are used to obtain the equations of motion of the continuum after discretizing it with finite beam elements. The equations of motion are solved to get the frequency response function and modal damping ratio. The process may be applied to study the dynamic behaviour of composite beams and rotors comprising of several viscoelastic layers. Dynamic behaviour of a composite beam, formed by concentric layers of steel and aluminium is studied as an example.