• Asian Pacific Journal of Cancer Prevention
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• 제14권7호
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• pp.4421-4426
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• 2013

Objective: The present study employed 5-aza-2'-deoxycytidine (5-Aza-CdR) to treat non-small cell lung cancer (NSCLC) cell line A549 to investigate the effects on proliferation and expression of the TFPI-2 gene. Methods: Proliferation was assessed by MTT assay after A549 cells were treated with 0, 1, 5, 10 ${\mu}mol/L$ 5-Aza-CdR, a specific demethylating agent, for 24, 48 and 72h. At the last time point cells were also analyzed by flow cytometry (FCM) to identify any change in their cell cycle profiles. Methylation-specific polymerase chain reaction (MSPCR), real time polymerase chain reaction(real-time PCR) and western blotting were carried out to determine TFPI-2 gene methylation status, mRNA expression and protein expression. Results: MTT assay showed that the growth of A549 cells which were treated with 5-Aza-CdR was significantly suppressed as compared with the control group (0 ${\mu}mol/L$ 5-Aza-CdR). After treatment with 0, 1, 5, 10 ${\mu}mol/L$ 5-Aza-CdR for 72h, FCM showed their proportion in G0/G1 was $69.7{\pm}0.99%$, $76.1{\pm}0.83%$, $83.8{\pm}0.35%$, $95.5{\pm}0.55%$ respectively (P<0.05), and the proportion in S was $29.8{\pm}0.43%$, $23.7{\pm}0.96%$, $15.7{\pm}0.75%$, $1.73{\pm}0.45%$, respectively (P<0.05), suggesting 5-Aza-CdR treatment induced G0/G1 phase arrest. MSPCR showed that hypermethylation in the promoter region of TFPI-2 gene was detected in control group (0 ${\mu}mol/L$ 5-Aza-CdR), and demethylation appeared after treatment with 1, 5, 10 ${\mu}mol/L$ 5-Aza-CdR for 72h. Real-time PCR showed that the expression levels of TFPI-2 gene mRNA were $1{\pm}0$, $1.49{\pm}0.14$, $1.86{\pm}0.09$ and $5.80{\pm}0.15$ (P<0.05) respectively. Western blotting analysis showed the relative expression levels of TFPI-2 protein were $0.12{\pm}0.01$, $0.23{\pm}0.02$, $0.31{\pm}0.02$, $0.62{\pm}0.03$ (P<0.05). TFPI-2 protein expression in A549 cells was gradually increased significantly with increase in the 5-Aza-CdR concentration. Conclusions: TFPI-2 gene promoter methylation results in the loss of TFPI-2 mRNA and protein expression in the non-small cell lung cancer cell line A549, and 5-Aza-CdR treatment could induce the demethylation of TFPI-2 gene promoter and restore TFPI-2 gene expression. These findings provide theoretic evidence for clinical treatment of advanced non-small cell lung cancer with the demethylation agent 5-Aza-CdR. TFPI-2 may be one molecular marker for effective treatment of advanced non-small cell lung cancer with 5-Aza-CdR.

• 시큐리티연구
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• 제26호
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• pp.121-144
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• 2011

현재 우리나라의 국가안전관리(위기재난관리)체계를 지탱하고 있는 기본법은 '재난 및 안전관리기본법'이다. 이러한 '재난 및 안전관리기본법' 하에서 우리나라의 국가안전관리체계는 위기와 재난을 효율적으로 관리하기 위하여 이른바 삼단체계(중앙정부, 시도, 시군구)를 채택하고 있다. 통합위기관리체계(IEM)는 일차적으로 지역 대응기관의 조정과 협력(Coordination and Cooperation)체계를 중시하는 개념이며, 특히 서로 동등한 위치에서 기관과 기관 간의 협력을 강조하고 있다. 이러한 통합위기관리체계의 흐름에 발맞추어 영국과 미국의 국가안전관리체계는, 지역사회 내의 지역대응기관들이 중앙정부나 연방정부의 직접적인 개입없이 일차적으로 시민보호와 관련된 문제(위기 및 재난)를 관리하는 시스템을 구축하고 있다. 다시 말해, 영국과 미국에서는 위기와 재난의 범위가 아주 광범위하고 복잡한 경우, 이로 인하여 중앙정부의 조정과 협력이 절실히 요구되는 때에만 비로소 중앙정부의 개입이 직접적으로 이루어지고 있는 것이다. 이와 반대로 한국의 국가안전관리체계는 지시와 통제(Command and Control) 중심의 중앙집권화 된 시스템을 채택하고 있기 때문에, 위기재난 발생시(중요도에 관계없이) 중앙 정부가 쉽게 지역단위의 지휘체계를 무시하고 이를 통제하고 간섭할 수 있다. 현대사회는 불확실성, 비친숙성 및 비예측성이 증폭되고 있는, 이른바 '위험사회(Risk Society)' 또는 '포스트모던 사회(Post-modern Society)'라고 일컬어진다. 이러한 불확실성의 시대에서 과연 우리의 하향식 국가안전관리체계가, '위험사회(Risk Society)'와 '포스트모던 사회(Post-modern Society)'의 불확실성, 비친숙성, 비예측성을 효과적으로 관리할 수 있는지 의문점이 제기된다. 이러한 맥락에서, 동 논문은 한국의 시민보호체계도 영국과 미국의 위기재난관리시스템과 마찬가지로 분권화된 조정과 협력모델(Coordination and Cooperation)로 변화해야 한다고 주장한다. 이를 위해서, 현행 '재난 및 안전관리기본법' 체계 하에서 우리나라의 시민보호체계 및 안전관리체계를 비판적으로 검토하고, 이를 토대로 국가안전관리체계의 발전 방향에 대한 정책적 시사점을 제시하고자 한다.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2011년도 정기총회 및 추계학술대회 논문집
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• pp.1049-1058
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• 2011

This paper suggests blending time adjusting method of braking command characteristics management and Set value test for optimizing of braking deceleration to enhance the precise position stopping. This method minimizes pneumatic-braking degree deviation by characteristics management, and secures braking stability at braking. By Set value test method, braking blending characteristics are analyzed accurately. And by optimal timing tuning at braking blending, It enhanced the precise position stopping with stabilization of deceleration To demonstrate the usefulness of these suggestion, I modeled for Deajeon Line #1. And through comparison with case of related companies, the proposed method which this paper suggested is proved to be superior to others.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2011년도 정기총회 및 추계학술대회 논문집
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• pp.1269-1274
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• 2011

To cut down the maintenance expenses at branch lines, the foreign developed countries have developed the low-cost signalling on low-density lines. These examples are FFB(FunkFahrBetrieb) Radio based train operation by German railways(Deutsche Bahn AG), ERTMS REGIONAL by Europe and LOCOPROL system using a GPS. Also, Korea is developing Information and Communication Technology based low-cost signalling on low-density lines. The characteristics of this system are directly controled switch and crossing by driver. For the purpose of this paper, we reviewed the business process for switch and crossing control. Specially, we compared and analysed both methods whether the driver takes control over the operation procedures of switch or the control message is carried by the command of the controller. And by utilizing the instrument of UML, I planned the switch according to the control authority and the operation procedures needed for the control of crossing.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1997년도 한국자동제어학술회의논문집; 한국전력공사 서울연수원; 17-18 Oct. 1997
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• pp.1820-1823
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• 1997

For the Cartesian space position controlled robot, it is required to have the accurate mapping from the Cartesian space to the joint space in order to command the desired joint trajectories correctly. since the actual mapping from Cartesian space to joint space is obtained at the joint coordinate not at the actuator coordinate, uncertainty in Jacobian can be present. In this paper, two feasible neural network schemes are proposed to compensate for the kinematic Jacobian uncertainties. Uncertainties in Jacobian can be compensated by identifying either actuator Jacobian off-line or the inverse of that in on-line fashion. the case study of the stenciling robot is examined.

• 한국지능시스템학회:학술대회논문집
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• 한국퍼지및지능시스템학회 2003년도 ISIS 2003
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• pp.304-306
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• 2003

In this paper, we propose the intelligent robot control technique for mobile robot using personal digital assistants (PDA). With the proposed technique, the mobile rebot can trace human at regular intervals by the remote control method with PDA. The mobile robot can recognize the distances between it and human whom the robot must follow with both multi-ultrasonic sensors and PC-camera and then, can inference the direction and velocity of itself to keep the given regular distances. In the first place, the mobile robot acquires the information about circumstances using ultrasonic sensor and PC-camera then secondly, transmits the data to PDA using wireless LAN communication. Finally, PDA recognizes the status of circumstances using the fuzzy logic and neural network and gives the command to mobile robot again.

• 한국수학교육학회지시리즈D:수학교육연구
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• 제11권2호
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• pp.143-151
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• 2007

Nowadays there are practically no mathematical courses in which Computer Algebra Systems (CAS) programs, such as MATHEMATlCA, Maple, and TI-89/92, are not used to some extent. However, generally the usage of these programs is reduced to illustration of computing processes: calculation of integrals, differentiation, solution of various equations, etc. This is obtained by usage of standard command of type: Solve [...] in MATHEMATICA. At the same time the main difficulties arise at teaching nonconventional mathematical courses such as coding theory, discrete mathematics, cryptography, Scientific computing, which are gaining the increasing popularity now. Now it is impossible to imagine a modern engineer not having basic knowledge in discrete mathematics, Cryptography, coding theory. Digital processing of signals (digital sound, digital TV) has been introduced in our lives.

• Journal of Communications and Networks
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• 제10권3호
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• pp.308-315
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• 2008

When operating in frequency-division duplex (FDD) mode, transmit beamforming in multiple-input single-output (MISO) wireless communication systems typically requires accurate knowledge of downlink channel state information (CSI) at the transmitter. In practical FDD systems, obtaining such downlink CSI at the transmitter is challenging, if not impractical. To circumvent such challenge and support user mobility, we present a new method for transmit beamforming based on simple beam-control commands (BCCs) in MISO FDD mobile systems. We then numerically evaluate the effects of BCC errors in terms of transmit power efficiency, system capacity, and outage probability.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2001년도 ICCAS
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• pp.148.4-148
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• 2001

This paper shows an autopilot design example with simulation results for a medium range surface-to-air missile used to intercept fast maneuver targets. The missile is assumed to use both aerodynamic fins and side thrusters to achieve fast time response. The steady-state maneuver capability of the missile is assumed to be enough at high altitude to engage usual maneuvering targets. Side thruster is used to get an extremely rapid acceleration response at high altitude where the missile´s aerodynamic control effectiveness is weak. The strategy of control design is firstly to employ side thrusters to achieve a rapid response and then to hand-over the control to the aerodynamic fins to maintain the desired acceleration command in the steady state ...

• Transactions on Control, Automation and Systems Engineering
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• 제3권4호
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• pp.236-241
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• 2001

Teach pendant is the most widely used means of robot teaching at present. Despite the difficulties of using the motion command buttons on the teach pendant, it is an economical, robust, and effective device for robot teaching task. This paper presents the development of a force/moment direction sensor named COSMO that can improve the teach pendant based robot teaching. Robot teaching experiment of a six axis commercial robot using the sensor is described where operator holds the sensor with a hand, and move the robot by pushing, pulling, and twisting the sensor in the direction of the desired motion. No prior knowledge of the coordinate system is required. The function of the COSMO sensor is to detect the presence f force and moment along the principal axes of the sensor coordinate system. The transducer used in the sensor is micro-switch, and this intuitive robot teaching can be implemented at a very low cost.