• BMB Reports
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• 제30권5호
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• pp.326-331
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• 1997

To construct a competitive ELISA standard curve for the detection of glucose-6-phosphate debydrogenase (G6PD), we used highly purified native G6PD (nG6PD) as both immobilized and soluble antigens and anti-G6PD serum raised against nG6PD as antibody. The polystyrene cuvettes coated with nG6PD were challenged with a mixture of a limiting amount of anti-G6PD serum and various doses of nG6PD as competitors followed by incubation with alkaline phosphatase-anti-IgG conjugate. The competitive ELISA did not exhibit the typical sigmoidal dose-response curve characteristic of competition immunoassays under the optimal concentrations of antigen and antibody. The soluble nG6PD used as competitor failed to effectively inhibit the binding of antibodies to the immobilized nG6PD. The addition of NADP, a cofactor of G6PD enzyme, to coating buffer used for immobilizing nG6PD to the cuvettes and PBS-Tween-BSA buffer for diluting competitors did not improve the inhibition of antibody binding to immobilized nG6PD by soluble n/G6PD. The addition of BSA to coating buffer did not increase inhibition, either. Surprisingly, when partially active G6PD (paG6PD), obtained by repeated freeze-thawing, was used as competitor, the antibody binding to either immobilized nG6PD or immobilized paG6PD was inhibited 49-58%. We conclude that an effective competitive ELISA system with nG6PD enzyme and anti-G6PD serum for the detection of G6PD may not be established due to the poor inhibition of antibody binding to immobilized nG6PD by soluble nG6PD under the present assay conditions and that the inhibition may be improved by using an inactivated enzyme as competitor regardless of the type of immobilized antigen used. These results imply that the immobilized nG6PD may undergo denaturation upon binding to the polystyrene cuvettes and that our anti-G6PD serum may recognize denatured enzyme better than active enzyme.

• 한국진공학회지
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• 제20권6호
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• pp.449-455
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• 2011

$In_{0.4}Al_{0.6}As$ 버퍼층의 성장온도 변화에 따른 $In_{0.5}Ga_{0.5}As/In_{0.5}Al_{0.5}As$ 다중양자우물(multiple quantum wells, MQWs)의 광학적 특성을 포토루미네션스(photoluminescence, PL)와 시간분해 포토루미네션스(time-resolved PL, TRPL) 측정을 이용하여 분석하였다. $In_{0.4}Al_{0.6}As$ 버퍼층은 기판의 온도를 $320^{\circ}C$에서 $580^{\circ}C$까지 다양하게 변화시키며 $1{\mu}m$ 성장하였으며, 그 위에 $In_{0.5}Al_{0.5}As$ 층을 $480^{\circ}C$에서 $1{\mu}m$ 성장한 후 InGaAs/InAlAs MQWs을 성장하였다. MQWs는 6-nm, 4-nm, 그리고 2.5-nm 두께의 $In_{0.5}Ga_{0.5}As$ 양자우물과 10-nm 두께의 $In_{0.5}Al_{0.5}As$ 장벽으로 이루어졌다. 4-nm QW과 6-nm QW로부터 PL 피크가 나타났으나, $In_{0.4}Al_{0.6}As$ 성장온도 변화가 가장 큰($320^{\circ}C$에서 $580^{\circ}C$까지 변화) 시료는 6-nm QW에서의 PL 피크만 나타났다. 낮은 온도($320^{\circ}C$에서 $480^{\circ}C$까지 변화)에서 성장한 $In_{0.4}Al_{0.6}As$ 버퍼층 위에 성장한 MQWs의 PL 특성이 우수하게 나타났다. 발광파장에 따른 TRPL 결과로 4-nm QW과 6-nm QW에서의 캐리어 소멸시간을 얻었다.

• 공업화학
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• 제26권4호
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• pp.483-488
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• 2015

본 연구에서는 제주도에서 채집한 Hizikia fusiformis biochar를 이용하여 수용액 상에서 $Cr^{6+}$과 $As^{3+}$ 중금속의 흡착 특성을 평가하였다. $Cr^{6+}$과 $As^{3+}$ 흡착에 있어서 최적 pH는 각각 pH 2와 pH 6이었다. 동역학적 실험 결과, 대부분의 중금속이 처음 100 min 동안 흡착이 되었으며, 300 min 이후 평형에 도달하였다. 또한, 해초 biochar의 $Cr^{6+}$과 $As^{3+}$ 중금속 흡착은 유사 1차 모델과 2차 모델에서 모두 잘 부합하고 있는 것으로 나타났다. 평형 흡착 실험 결과는 Langmuir 모델에 잘 부합했고, $Cr^{6+}$ (25.91 mg/g)이 $As^{3+}$ (16.54 mg/g)보다 흡착량이 높았다. 본 연구 결과를 통해, 오염된 환경에서 해초 biochar는 $Cr^{6+}$ 및 $As^{3+}$ 중금속의 효과적인 흡착제임을 보였다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권9호
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• pp.3961-3968
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• 2015

Background: Variants of human papillomavirus (HPV) show more oncogenicity than do prototypes. The HPV16 Asian variant (HPV16As) plays a major role in cervical cancer of Asian populations. Some amino acid changes in the E6 protein of HPV16 variants affect E6 functions such as p53 interaction and host immune surveillance. This study aimed to investigate activities of HPV16As E6 protein on modulation of expression of miRNA-21 as well as interferon regulatory factors (IRFs) 1, 3, 7 and c-fos. Materials and Methods: Vectors expressing E6 protein of HPV16As (E6D25E) or HPV16 prototype (E6Pro) were constructed and transfected into C33A cells. HCK1T cells expressing E6D25E or E6Pro were established by transducing retrovirus-containing E6D25E or 16E6Pro. The E6AP-binding activity of E6 and proliferation of the transfected C33A cells were determined. MiR-21 and mRNA of interesting genes were detected in the transfected C33A cells and/or the HCK1T cells, with or without treatment by culture medium from HeLa cells (HeLa-CM). Results: E6D25E showed binding activity with E6AP similar to that of E6Pro. Interestingly, E6D25E showed a higher activity of miR-21 induction than did E6Pro in C33A cells expressing E6 protein. This result was similar to the HCK1T cells expressing E6 protein, with HeLa-CM treatment. The miR-21 up-regulation significantly corresponded to its target expression. Different levels of expression of IRFs were also observed in the HCK1T cells expressing E6 protein. Interestingly, when treated with HeLa-CM, IRFs 1, 3 and 7 as well as c-fos were significantly suppressed in the HCK1T cells expressing E6D25E, whereas those in the HCK1T cells expressing E6Pro were induced. A similar situation was seen for IFN-${\alpha}$ and IFN-${\beta}$. Conclusions: E6D25E of the HPV16As variant differed from the E6 prototype in its activities on epigenetic modulation and immune surveillance and this might be a key factor for the important role of this variant in cervical cancer progression.

• Journal of Microbiology
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• 제45권4호
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• pp.318-325
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• 2007

Glucose 6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) in Deinococcus radiophilus, an extraordinarily UV-resistant bacterium, was investigated to gain insight into its resistance as it was shown to be involved in a scavenging system of superoxide $(O_2^{-1})$ and peroxide $(O_2^{-2})$ generated by UV and oxidative stresses. D. radiophilus possesses two G6PDH isoforms: G6PDH-1 and G6PDH-2, both showing dual coenzyme specificity for NAD and NADP. Both enzymes were detected throughout the growth phase; however, the substantial increase in G6PDH-1 observed at stationary phase or as the results of external oxidative stress indicates that this enzyme is inducible under stressful environmental conditions. The G6PDH-1 and G6PDH-2 were purified 122- and 44-fold (using NADP as cofactor), respectively. The purified G6PDH-1 and G6PDH-2 had the specific activity of 2,890 and 1,033 U/mg protein (using NADP as cofactor) and 3,078 and 1,076 U/mg protein (using NAD as cofactor), respectively. The isoforms also evidenced distinct structures; G6PDH-1 was a tetramer of 35 kDa subunits, whereas G6PDH-2 was a dimer of 60kDa subunits. The pIs of G6PDH-1 and G6PDH-2 were 6.4 and 5.7, respectively. Both G6PDH-1 and G6PDH-2 were inhibited by both ATP and oleic acid, but G6PDH-1 was found to be more susceptible to oleic acid than G6PDH-2. The profound inhibition of both enzymes by ${\beta}-naphthoquinone-4-sulfonic$ acid suggests the involvement of lysine at their active sites. $Cu^{2+}$ was a potent inhibitor to G6PDH-2, but a lesser degree to G6PDH-1. Both G6PDH-1 and G6PDH-2 showed an optimum activity at pH 8.0 and $30^{\circ}C$.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 1999년도 추계학술대회 논문집
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• pp.29-32
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• 1999

$GaF_3$ films were directly grown on p' and p-type GaAs(100) substrates using a $SF_6$ plasma discharge system. GaAs MIS(Meta1-Insulator-Semiconductor) capacitor was successfully fabricated for about 1 hour at temperature $290^{\circ}C$ using the as-grown $GaF_3$ films. The as-grown films on p'-GaAs exhibited a current density of less than 6.68 $\times$ $1O^{-9}$ A/$cm^2$ at a breakdown field of 500kV/cm and a refractive index of 2.0 ~ 2.3 at a wavelength of 632.8 nm. The dielectric constant was about 5 derived from 1 MHz capacitance-voltage (C-V) measurements. Dielectric dispersion of the fluoridated films on p'-GaAs measured ranged from 100 Hz to 10 MHz was not observed.

• 한국미생물·생명공학회지
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• 제21권5호
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• pp.421-427
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• 1993

Some interleukin 6 (IL-60 transcription control factors were resported as the regulator of IL-6 expression. A nuclear protein bound to interleukin 1 (IL-1) responsive element in the IL-6 promoter region was named NF-IL6 (nuclear factor for IL-6). This NF-IL6 was known to be very imporant as a transcription factor for various immuno-protein as well as for IL-6. The human NF-IL6 genes were transfected into the mouse L cells under the metallothionein promoter (MT promoter) to establish a model system for the expression of foreign gene in the mammalian cell line.

• 대한안전경영과학회지
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• 제5권2호
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• pp.215-223
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• 2003

GE, as well as Motorola, TI, HP, ABB, and other companies, which have several suppliers from all of the world, adopted an innovation program called 6 Sigma, in order to make fundamental changes in the way they operated to fulfill customers' expectations and to evaluate and control their suppliers. Recently, as the 6 Sigma is being recognized as elementary and strategic means to improve the effectiveness of SCM(Supply Chain Management), there may be few business who does not operate 6 Sigma innovation program. Under such a circumstance, this study is aimed at expediting 6 Sigma pervasion by considering supplier evaluating scheme. It is recognized that this evaluating mechanism can be used as a great tool for the everlasting run of 6 Sigma in Korea.

• 한국정보처리학회논문지
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• 제4권10호
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• pp.2521-2532
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• 1997

월드 와이드 웹(WWW)과 MBone 응용 등의 출현으로 인터넷 이용자가 급속히 증가되면서 인터넷 주소 공간의 확장과 멀티미디어 응용을 지원할 수 있도록 기존 인터넷 프로토콜의 개선이 요구됨에 따라 IETF에서는 차세대 인터넷 망 계층 프로토콜로써 IPv6를 개발하였다. 이 논문에서는 IPv6 프로토콜을 Windows NT의 커널 내부에 프로토콜 드라이버로 구현한 내용을 기술하고 있다. 본 연구에서는 IPv6 호스트로 동작되기 위해 필수적으로 요구되는 IPv6 헤더 처리기능, IPv6 주소처리기능, 제어메세지 처리기능, 그룹관리 메세지 처리기능, 이웃탐색 기능이 구현 및 시험되었다. 구현된 IPv6 프로토콜 드라이버 모듈은 하부 통신망 접속 카드와 표준 인터페이스인 NDIS를 통해 접속되며, 디스패치 함수화 Lower-Edge 함수를 이용하여 커널 내부에서 동작하는 드라이버 모듈을 상위 사용자 응용 및 하부 NDIS와 접속시키는 형태로 구현하였다. 구현된 IPv6 프로토콜 드라이버는 커널 모드에서 구현됨으로써 향상된 성능을 제공하며, 다이나믹 링크 라이브러리 형태로 사용자 인터페이스를 제공하므로 응용 프로그램 개발자들이 손쉽게 사용할 수 있도록 하였다.

• 한국통신학회논문지
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• 제37B권9호
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• pp.806-813
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• 2012

클라우드 컴퓨팅 환경에서 Infrastructure as a Services (IaaS), Platform as a Services (PaaS), Software as a Services (SaaS), Desktop as a Services (DaaS) 등 다양한 솔루션들이 지속적으로 제공되고 있다. 현재는 사용자 단말이 클라우드 서비스를 제공받으면서 이동성을 보장하기 위한 솔루션으로 Mobile as a Services(MaaS)가 가장 많은 주목을 받고 있다. 사용자는 이동을 하면서도 클라우드에 있는 데이터 및 어플리케이션에 대한 접근과 이용이 가능해야 한다. 다시 말해 모바일 Thin-Client 환경에서 클라우드와 통신, 이동성 지원은 필수 요소이다. 모바일 단말의 이동성을 지원하기 위해 MobileIPv6 (MIPv6) 및 Proxy Mobile IPv6 (PMIPv6)가 소개되면서 많은 연구가 진행되고 있다. 또한, PMIPv6에 대한 연구는 도메인 내에 패킷 손실을 우려한 최적 경로 설정, 빠른 핸드 오버 등의 개선방안이 많이 제시된 바 있다. 본 논문은 모바일 Thin-Client 지원을 위해 PMIPv6 및 클라우드 연동 시스템을 제안한다. 제안한 시스템에서 모바일 Thin-Client가 서비스를 지원받기 위해 Replica서버를 이용하여 원활한 서비스를 제공하는 기법을 제안하며 성능평가를 통하여 기존 PMIPv6에서 핸드오버에 대한 데이터 비용을 비교 분석할 것이다.