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Growth and Nutrient Utilization in Buffalo Calves Fed Urea-ammoniated Wheat Straw and Hydrochloric Acid plus Urea Treated Wheat Straw

  • Nair, P.V.;Verma, A.K.;Dass, R.S.;Mehra, U.R.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.5
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    • pp.682-686
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    • 2002
  • Ten growing male buffalo calves (aged 6-8 months, average body weight $88.2{\pm}0.57$ kg were divided randomly into two groups. Animals in group I were fed on concentrate mixture A (CP 20.2%, TDN 77.4%) and urea- ammoniated wheat straw (UAWS) while the animals in group II were fed on concentrate mixture B (CP 17.9%, TDN 77.6%) and HCl plus urea treated wheat straw (HCl UAWS) to meet their nutrient requirement for 500 g gain/d as per Kearl (1982). This feeding practice lasted for 120 days, during which fortnightly body weight were recorded to assess their growth rate. A metabolism trial was conducted after 90 days of experimental feeding to compare the digestibility of nutrients, their balance, plane of nutrition and relative cost of feeding in two groups of animals. Results revealed a significant increase in the CP content of ammoniated wheat straw due to addition of HCl viz 12.1% from 7.5%. There was a decrease in the intake of DM (p<0.05), OM (p<0.05), EE (p<0.05), NDF (p<0.01), ADF (p<0.01), cellulose (p<0.01) and hemicellulose (p<0.01) in group II as compared to group I. The digestibility (%) of DM, OM and CP was significantly (p<0.01) more in group II, whereas the digestibility (%) was significantly more for NDF (p<0.05) and hemicellulose (p<0.01) in group I than group II. There was no significant difference in the N, Ca and P balance in two groups. Intake of total DM (g/d) or (g/kg $W^{0.75}$) was significantly (p<0.01) more in group I as compared to group II, whereas the intake of DCP and TDN (g/d or g/kg $W^{0.75}$) was alike in two groups. The total body weight gain (kg), average daily gain (g/d) and feed conversion efficiency were significantly (p<0.01) more in UAWS fed group as compared to HCl UAWS fed group. Feeding cost (Rs./kg. weight gain) was significantly (p<0.05) more in group II as compared to group I. It is concluded that HCl UAWS is not suitable for the feeding of growing buffalo calves as it reduced the growth rate in comparison to UAWS fed buffalo calves.

Effect of Feeding Bypass Protein with Urea Treated Grass on the Performance of Red Kandhari Calves

  • Wankhede, S.M.;Kalbande, V.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.7
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    • pp.970-973
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    • 2001
  • An experiment was carried out to assess the effect of feeding concentrate mixtures varying in bypass protein levels with urea-treated or untreated grass on the performance of twelve Red Kandhari calves (14 months of age and 78.15 kg body weight) for a period of 75 days. Dry grass was treated with 4 percent urea solution and ensiled for 30 days. The CP ($N{\times}6.25$) content in urea treated grass increased from 3.96 to 8.89 percent. Two iso-caloric and iso-nitrogenous concentrate mixtures (CM-I and CM-II) varying in RDP to UDP ratio viz., 65:35 and 55.45 were prepared. The calves in control group ($T_1$) were fed concentrate mixture-I with ad libitum untreated dry grass and those in experimental group ($T_2$) were fed concentrate mixture-II with ad libitum urea treated dry grass. The dry matter consumption in group $T_2$ was significantly (p<0.01) higher as compared to group $T_1$. The total DMI in $T_1$ and $T_2$ was 146.92 and 166.95 kg respectively, whereas the DMI per day and per 100 kg body weight was 1.94 and 2.22 and 1.90 and 2.35 kg, respectively. The average total gain in body weight (kg) and average daily gain (g) of calves in $T_2$ was significantly (p<0.01) higher as compared to those in $T_1$ the values being 28.66, 18.33 and 382.16, 244.44, respectively. Feed efficiency in terms of kg DM per kg gain in body weight was significantly (p<0.01) lower in group $T_1$ than in $T_2$. The cost of feed per kg gain in body weight for $T_2$ and $T_1$ group was Rs. 21.14, 28.22, respectively. The digestibility coefficients of DM, CP, EE, CF, NFE, NDF and ADF were 59.60, 57.50, 53.00, 65.04, 45.82, 48.48, 52.48 and 55.73 for $T_1$ group. The coressponding values were 68.78, 67.80, 59.83, 71.41, 49.93, 53.37 and 57.81, respectively for $T_2$ group. The digestibility coefficients for all the proximate principles in $T_2$ were significantly (p<0.01) higher as compared to $T_1$. However, NDF and ADF digestibilities were not significantly different. Nutritive value determined in terms of DCP and TDN for The experimental ration was significantly (p<0.01) higher than control ration, the values being 7.32 and 47.34 and 9.39 and 52.40% respectively. The blood urea nitrogen levels at 0, 3 and 6 h interval after feeding were significantly (p<0.01) lower in calves fed experiment ration as compared to control. The overall results indicated that in Red Kandhari calves an optimum growth can be economically achieved by feeding 4 percent urea treated dry and mature grass as basal roughage supplemented with a concentrate mixture containing 20 percent CP, 70% TDN and 45% UDP/bypass protein.

Clinical Analysis Of Ventricular Septal Defect (심실중격결손증의 외과적 고찰)

  • Seong, Suk-Hwan;Suh, Kyung-Pill
    • Journal of Chest Surgery
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    • v.15 no.1
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    • pp.90-97
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    • 1982
  • Two hundred one patients of ventricular septal defect, which were operated at Seoul National University Hospital, were analysed on clinical background during the period from January, 1975 to December, 1980. The results were as follows: 1. Of the 201 patients, 118 patients were male [58.7%] and 83 patients were female [41.3%]. Their age ranged from 15 months to 40 years, and the mean age was 8.7 years. 40% of the patients were between 4 and 8 years. 2. The most common symptoms showed frequent U RI and exertional dyspnea. 3. On Kirklin`s anatomical classification, type I constituted 26.9%, type II 58.2%, type III 12.4%, and type IV 1.0%. We showed marked increased incidence of type I VSD as compared to Caucasians* 4. 46 cases were associated with other congenital cardiac diseases. They were PDA [13 cases], AI[11 ], ASD[6], PS[10], MI[4], and Double aortic arch [1]. 5. In 128 patients, who had complete hemodynamic data and were not associated with other congenital cardiac diseases, an attempt was made to correlate the EKG findings with the hemodynamic data, and defect size with the hemodynamic data. The children had variable distribution of PA syst. pr. and Rp/Rs. But most of adults had $R_P$/$R_S$of 0.15 or less. As $P_P$/$P_S$increased, the rate of operative complication increased also. 6. When a normal EKG pattern was present, $Q_P$/$Q_S$and $R_P$/$R_S$and $P_P$/$P_S$were relatively low. When EKG findings were LVH pattern, there was diastolic volume overload to left ventricle. As RVH, there was systolic pressure overload to right ventricle. And as BVH, there was mixed pattern of diastolic volume overload to left ventricle and systolic pressure overload to right ventricle. 7. Among patients in defect was less than 1 $cm^2$ per $M^2$ of BSA, $Q_P$/$Q_S$was less than 2:1, and $R_P$/$R_S$less than 0.25, and PAsyst. pr. less than 50 mmHg, and $P_P$/$P_S$was less than 0.5. But patients with the defect greater than 1 $cm^2$ per $M^2$ of BSA had no correlationship between $Q_P$/$Q_S$, $R_P$/$R_S$, PAsyst. pr. and defect size in each other. Most of patients with the defect greater than 2 $cm^2$/$M^2$ BSA, $R_P$/$R_S$was greater than 0.5. 8. Operative mortality rate was 9.5% [19 cases] among 201 patients. And complication rate including mortality rate was 22.9% [46 cases].

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Development of Acquisition and Analysis System of Radar Information for Small Inshore and Coastal Fishing Vessels - Position Tracking and Real-Time Monitoring- (연근해 소형 어선의 레이더 정보 수록 및 해석 시스템 개발 -위치 추적 및 실시간 모니터링 -)

  • 이대재;김광식;신형일;변덕수
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.39 no.4
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    • pp.337-346
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    • 2003
  • This paper describes on the system and method for automatically tracking and real-time monitoring the position of target ships relative to the own ship using a PC based radar system that displays radar images and electronic charts together on a single PC screen. This system includes a simulator for generating the GGA and VTG information of target ships and a simulator for generating the TTM and OSD outputs from a ARPA radar and then host computer accepts NMEA0183 sentences on the maneuvering information of target ships from these simulators. The results obtained are summarized as follows;1. The system developed this study can be used as a range finder for measuring the distance between two ships and as a device for providing the maneuvering information such as distance and bearing to target ships from own ship on ECS screen. 2. From the result of position tracking for a selected target ship tracked with an update rate of 5 seconds using the $\alpha$-$\beta$ tracker, we concluded that the smoothing effect by the $\alpha$-$\beta$tracker was very effective and stable except in the time interval until about one minute after the target is detected. 3. From the fact that the real-time maneuvering information of tracked ship targets via a local area network (LAN) from a host computer installed a radar target extractor was successfully transferred to various monitoring computers of ship, we concluded that this system can be used as a sub-monitoring system of ARPA radar.

Immunological Characterization of Full and Truncated Recombinant Clones of ompH(D:4) Obtained from Pasteurella multocida (D:4) in Korea

  • Kim, Young-Hwan;Cheong, Ki-Young;Shin, Woo-Seok;Hong, Sung-Youl;Woo, Hee-Jong;Kwon, Moo-Sik
    • Journal of Microbiology and Biotechnology
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    • v.16 no.10
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    • pp.1529-1536
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    • 2006
  • We cloned a gene of ompH(D:4) from pigs infected with P. multocida D:4 in Korea [16]. The gene is composed of 1,026 nucleotides coding 342 amino acids (aa) with a signal peptide of 20 aa (GenBank accession number AY603962). In this study, we analyzed the ability of the ompH(D:4) to induce protective immunity against a wild-type challenge in mice. To determine appropriate epitope(s) of the gene, one full and three different types of truncated genes of the ompH(D:4) were constructed by PCR using pET32a or pRSET B as vectors. They were named ompH(D:4)-F (1,026 bp [1-1026] encoding 342 aa), ompH(D:4)-t1 (693 bp [55-747] encoding 231 aa), ompH(D:4)-t2 (561 bp [187-747] encoding 187 aa), and ompH(D:4)-t3 (540 bp [487-1026] encoding 180 aa), respectively. The genes were successfully expressed in Escherichia coli BL21(DE3). Their gene products, polypeptides, OmpH(D:4)-F, -t1, -t2, and -t3, were purified individually using nickel-nitrilotriacetic acid (Ni-NTA) affinity column chromatography. Their $M_rs$ were determined to be 54.6, 29, 24, and 23.2 kDa, respectively, using SDS-PAGE. Antisera against the four kinds of polypeptides were generated in mice for protective immunity analyses. Some $50{\mu}g$ of the four kinds of polypeptides were individually provided intraperitoneally with mice (n=20) as immunogens. The titer of post-immunized antiserum revealed that it grew remarkably compared with pre-antiserum. The lethal dose of the wild-type pathogen was determined at $10{\mu}l$ of live P. multocida D:4 through direct intraperitoneal (IP) injection, into post-immune mice (n=5, three times). Some thirty days later, the lethal dose ($10{\mu}l$) of live pathogen was challenged into the immunized mouse groups [OmpH(D:4)-F, -t1, -t2, and -t3; n=20 each, two times] as well as positive and negative control groups. As compared within samples, the OmpH(D:4)-F-immunized groups showed lower immune ability than the OmpH(D:4)-t1, -t2, and -t3. The results show that the truncated-OmpH(D:4)-t1, -t2, and -t3 can be used for an effective vaccine candidate against swine atrophic rhinitis caused by pathogenic P. multocida (D:4) isolated in Korea.

Strength and CO2 Reduction of Fiber-Reinforced Cementitious Composites with Recycled Materials (자원순환형 재료를 사용한 섬유보강 시멘트 복합체(FRCCs)의 강도 및 CO2 저감에 관한 연구)

  • Lee, Jong-Won;Kim, Sun-Woo;Park, Wan-Shin;Jang, Young-Il;Yun, Hyun-Do
    • Journal of the Korea Concrete Institute
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    • v.29 no.4
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    • pp.379-387
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    • 2017
  • The objective of this study is to develop sustainable PVA fiber-reinforced cementitious composites (FRCCs) that could exhibit comparable strength level to normal PVA FRCCs with no recycled materials. To evaluate mechanical properties of the FRCCs, compressive, flexural and direct tensile tests were conducted. In addition to the test, to calculate amount of carbon dioxide ($CO_2$) emission at the stage of manufacturing the FRCCs, life cycle inventory data base (LCI DB) were referenced from domestic and Japan. From the test results, the mechanical properties such as compressive, flexural and direct tensile strengths were decreased as the replacement ratio of recycled materials increased. And it was determined that the amount of $CO_2$ emission was reduced for the specimens with higher water-binder ratio (W/B) and replacement ratios. It was also found that binder intensity ($B_i$) value was higher as replacement ratio of fly ash (FA) increased. This result means that larger amount of FA is need to deliver one unit of a given performance indicator (1 MPa of strength) of FRCCs compared to that of ordinary portland cement (OPC). As a result, it could be concluded that FRCCs with W/B 45% replaced by FA 25% and recycled sand (RS) 25% is desirable for both target performance and $CO_2$ emission.

Development of PC-based and portable high speed impedance analyzer for biosensor (바이오센서를 위한 PC 기반의 휴대용 고속 임피던스 분석기 개발)

  • Kim, Gi-Ryon;Kim, Gwang-Nyeon;Heo, Seung-Deok;Lee, Seung-Hoon;Choi, Byeong-Cheol;Kim, Cheol-Han;Jeon, Gye-Rok;Jung, Dong-Keun
    • Journal of Sensor Science and Technology
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    • v.14 no.1
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    • pp.33-41
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    • 2005
  • For more convenient electrode-electrolyte interface impedance analysis in biosensor, a stand-alone impedance measurement system is required. In our study, we developed a PC-based portable system to analyze impedance of the electrochemical cell using microprocessor. The devised system consists of signal generator, programmable amplifiers, A/D converter, low pass filter, potentiostat, I/V converter, microprocessor, and PC interface. As a microprocessor, PIC16F877 which has the processing speed of 5 MIPS was used. For data acquisition, the sampling rate at 40 k samples/sec, resolution of 12 bit is used. RS-232 with 115.2 kbps speed is used for the PC communication. The square wave was used as stimuli signal for impedance analysis and voltage-controlled current measurement method of three-electrode-method were adopted. Acquired voltage and current data are calculated to multifrequency impedance signal after Fourier transform. To evaluate the implemented system, we set up the dummy cell as equivalent circuit of which was composed of resistor, parallel circuit of capacitor and resistor connected in parallel and measured the impedance of the dummy cell; the result showed that there exist accuracy within 5 % errors and reproduction within 1 % errors compared to output of Hioki LCR tester and HP impedance analyzer as a standard product. These results imply that it is possible to analyze electrode-electrolyte interface impedance quantitatively in biosensor and to implement the more portable high speed impedance analysis system compared to existing systems.

Application of Computer-Aided Diagnosis a using Texture Feature Analysis Algorithm in Breast US images (유방 초음파영상에서 질감특성분석 알고리즘을 이용한 컴퓨터보조진단의 적용)

  • Lee, Jin-Soo;Kim, Changsoo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.1
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    • pp.507-515
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    • 2015
  • This paper suggests 6 cases of TFA parameters algorithm(Mean, VA, RS, SKEW, UN, EN) to search for the detection of recognition rates regarding breast disease using CAD on ultrasound images. Of the patients who visited a university hospital in Busan city from August 2013 to January 2014, 90 cases of breast ultrasound images based on the findings in breast US and pathology were selected. $50{\times}50$ pixel size ROI was selected from the breast US images. After pre-processing histogram equalization of the acquired test images(negative, benign, malignancy), we calculated results of TFA algorithm using MATLAB. As a result, in the TFA parameters suggested, the disease recognition rates for negative and malignancy was as high as 100%, and negative and benign was approximately 83~96% for the Mean, SKEW, UN, and EN. Therefore, there is the possibility of auto diagnosis as a pre-processing step for a screening test on breast disease. A additional study of the suggested algorithm and the responsibility and reproducibility for various clinical cases will determine the practical CAD and it might be possible to apply this technique to range of ultrasound images.

Interleukin-12 and Interleukin-6 Gene Polymorphisms and Risk of Bladder Cancer in the Iranian Population

  • Ebadi, Nader;Jahed, Marzieh;Mivehchi, Mohamad;Majidizadeh, Tayebeh;Asgary, Mojgan;Hosseini, Seyed Ali
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.18
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    • pp.7869-7873
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    • 2014
  • Interleukin-12 (IL-12) as an antitumor and interleukin-6 (IL-6) as an inflammatory cytokine, are immunomodulatory products that play important roles in responses in cancers and inflammation. We tested the association between two polymorphisms of IL-12(1188A>C; rs3212227) and IL-6 (-174 C>G) and the risk of bladder cancer in 261 patients and 251 healthy individuals. We also investigated the possible association of these SNPs in patients with high-risk jobs and smoking habits with the incidence of bladder cancer. The genotype distributions of IL-6 (-174 C/G) genotype were similar between the cases and the control groups; however, among patients with smoking habits, the association between IL-6 gene polymorphism and incidence of bladder cancer was significant. After a control adjustment for age and sex, the following results were recorded: CC genotype (OR= 2.11, 95%CI=1.56-2.87, p=0.007), GC genotype (OR=2.18, 95%CI=1.16-4.12, p=0.014) and GC+CC (OR=2.6, 95%CI=1.43-4.47, p=0.011). A significant risk of bladder cancer was observed for the heterozygous genotype (AC) of IL-12 (OR=1.47, 95%CI=1.01-2.14, p=0.045) in all cases, and among smokers (AC) (OR=3.13, 95%CI=1.82-5.37, p=0.00014), combined AC+CC (OR=3.05, 95%CI=1.8-5.18, p=0.000015). Moreover among high risk job patients, there was more than a 3-fold increased risk of cancer in the carriers of IL-12 beta heterozygous (OR=3.7, 95%CI=2.04-6.57, p=0.000056) and combined AC+CC(OR=3.29, 95%CI=1.58-5.86, p=0.00002) genotypes as compared with the AA genotype with low-risk jobs. As a conclusion, this study suggests that IL-12(3'UTR A>C) and IL-6 (-174 C>G) genotypes are significantly associated with an increased risk of bladder cancer in the Iranian population with smoking habits and/or performing high-risk jobs.

Current status and prospects of plant diagnosis and phenomics research by using ICT remote sensing system (ICT 원격제어 system 이용 식물진단, Phenomics 연구현황 및 전망)

  • Jung, Yu Jin;Nou, Ill Sup;Kim, Yong Kwon;Kim, Hoy Taek;Kang, Kwon Kyoo
    • Journal of Plant Biotechnology
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    • v.43 no.1
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    • pp.21-29
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    • 2016
  • Remote Sensing (RS) is a technique to obtain necessary information in a non-contact and non-destructive method by using various sensors on the surface, water or atmospheric phenomena. These techniques combine elements such as sensors, and platform and information communication technology (ICT) for mounting the sensor. ICT has contributed significantly to the success of smart agriculture through quantification and measurement of environmental factors and information such as weather, crop and soil management to distribution and consumption stage, as well as the production stage by the cloud computer. Remote sensing techniques, including non-destructive non-contact bioimaging (remote imaging) is required to measure the plant function. In addition, bioimaging study in plant science is performed at the gene, cellular and individual plant level. Recently, bioimaging technology is considered the latest phenomics that identifies the relationship between the genotype and environment for distinguishing phenotypes. In this review, trends in remote sensing in plants, plants diagnostics and response to environment and status of plants phonemics research were presented.