• Title/Summary/Keyword: ART-1

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The frequency of defective genes in vif and vpr genes in 20 hemophiliacs is associated with Korean Red Ginseng and highly active antiretroviral therapy: the impact of lethal mutations in vif and vpr genes on HIV-1 evolution

  • Cho, Young Keol;Kim, Jung-Eun
    • Journal of Ginseng Research
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    • v.45 no.1
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    • pp.149-155
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    • 2021

  • Background: We have reported that internal deletions in the nef, gag, and pol genes in HIV-1-infected patients are induced in those treated with Korean Red Ginseng (KRG). KRG delays the development of resistance mutations to antiretroviral drugs. Methods: The vif-vpr genes over 26 years in 20 hemophiliacs infected with HIV-1 from a single source were sequenced to investigate whether vif-vpr genes were affected by KRG and KRG plus highly active antiretroviral therapy (ART) (hereafter called GCT) and compared the results with our previous data. Results: A significantly higher number of in-frame small deletions were found in the vif-vpr genes of KRG-treated patients than at the baseline, in control patients, and in ART-alone patients (p < 0.001). These were significantly reduced in GCT patients (p < 0.05). In contrast, sequences harboring a premature stop codon (SC) were more significant in GCT patients (10.1%) than in KRG-alone patients, control (p < 0.01), and ART-alone patients (p = 0.078 for peripheral blood mononuclear cells). The proportion of SC in Vpr was similar to that in Vif, whereas the proportion of sequences revealing SC in the env-nef genes was significantly lower than that in the pol-vif-vpr genes (p < 0.01). The genetic distance was 1.8 times higher in the sequences harboring SC than in the sequences without SC (p < 0.001). Q135P in the vif gene is significantly associated with rapid progression to AIDS (p < 0.01). Conclusion: Our data show that KRG might induce sD in the vif-vpr genes and that vif-vpr genes are similarly affected by lethal mutations.

  • https://doi.org/10.1016/j.jgr.2020.03.003 인용 PDF KSCI

Korean Red Ginseng slows coreceptor switch in HIV-1 infected patients

  • Young-Keol Cho;Jung-Eun Kim;Jinny Lee
    • Journal of Ginseng Research
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    • v.47 no.1
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    • pp.117-122
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    • 2023

  • Background: Human immunodeficiency virus-1 (HIV-1) that binds to the coreceptor CCR5 (R5 viruses) can evolve into viruses that bind to the coreceptor CXCR4 (X4 viruses), with high viral replication rates governing this coreceptor switch. Korean Red Ginseng (KRG) treatment of HIV-1 infected patients has been found to slow the depletion of CD4+ T cells. This study assessed whether the KRG-associated slow depletion of CD4+ T cells was associated with coreceptor switching. Methods: This study included 146 HIV-1-infected patients naïve to antiretroviral therapy (ART) and seven patients receiving ART. A total of 540 blood samples were obtained from these patients over 122 ± 129 months. Their env genes were amplified by nested PCR or RT-PCR and subjected to direct sequencing. Tropism was determined with a 10% false positive rate (FPR) cutoff. Results: Of the 146 patients naïve to ART, 102 were KRG-naïve, and 44 had been treated with KRG. Evaluation of initial samples showed that coreceptor switch had occurred in 19 patients, later occurring in 38 additional patients. There was a significant correlation between the amount of KRG and FPR. Based on initial samples, the R5 maintenance period was extended 2.35-fold, with the coreceptor switch being delayed 2.42-fold in KRG-treated compared with KRG-naïve patients. The coreceptor switch occurred in 85% of a homogeneous cohort. The proportion of patients who maintained R5 for ≥10 years was significantly higher in long-term slow progressors than in typical progressors. Conclusion: KRG therapy extends R5 maintenance period by increasing FPR, thereby slowing the coreceptor switch.

  • https://doi.org/10.1016/j.jgr.2022.06.003 인용 PDF

Estimation of pattern classification vigilance parameter using neural network

  • Son, Jun-Hyug;Seo, Bo-Hyeok
    • Proceedings of the KIEE Conference
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    • 2004.05a
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    • pp.95-97
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    • 2004

  • This paper estimates Adaptive Resonance Theory 1(ART1) as a vigilance parameter of pattern clustering algorithm. Inherent characteristics of the model are analyzed. In particular the vigilance parameter ${\rho}$ and its role in classification of patterns is examined. Our estimates show that the vigilance parameter as designed originally does not necessarily increase the number of categories with its value but can decrease also. This is against the claim of solving the stability-plasticity dilemma. However, we have proposed a modified vigilance parameter estimate criterion which takes into account the problem of subset and superset patterns and stably categorizes arbitrarily many input patterns in one list presentation when the vigilance parameter is closer to one.

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