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Feasibility of Environmental DNA Metabarcoding for Invasive Species Detection According to Taxa (분류군별 외래생물 탐지를 위한 환경 DNA 메타바코딩 활용 가능성)

  • Yujin Kang;Jeongeun Jeon;Seungwoo Han;Suyeon Won;Youngkeun Song
    • Journal of Environmental Impact Assessment
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    • v.32 no.2
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    • pp.94-111
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    • 2023
  • In order to establish an effective management strategy for invasive species early detection and regular monitoring are required to assess their introduction or dispersal. Environmental DNA (eDNA) is actively applied to evaluate the fauna including the presence of invasive species as it has high detection sensitivity and can detect multiple species simultaneously. In Korea, the applicability evaluation of metabarcoding is being conducted mainly on fish, and research on other taxa is insufficient. Therefore, this study identified the feasibility of detecting invasive species in Korea using eDNA metabarcoding. In addition, to confirm the possibility of detection by taxa, the detection of target species was evaluated using four universal primers (MiFish, MiMammal, Mibird, Amp16S) designed for fish, mammals, birds, and amphibians. As a result, target species (Trachemys scripta, 3 sites; Cervus nippon, 3 sites; Micropterus salmoides, 7 sites; Rana catesbeiana, 4 sites) were detected in 17 of the total 55 sites. Even in the selection of dense sampling sites within the study area, there was a difference in the detection result by reflecting the ecological characteristics of the target species. A comparison of community structures (species richness, abundance and diversity) based on the presence of invasive species focused on M.salmoides and T.scripta, showed higher diversity at the point where invasive species were detected. Also, 1 to 4 more species were detected and abundance was also up to 1.7 times higher. The results of invasive species detection through metabarcoding and the comparison of community structures indicate that the accumulation of large amounts of monitoring data through eDNA can be efficiently utilized for multidimensional ecosystem evaluation. In addition, it suggested that eDNA can be used as major data for evaluation and prediction, such as tracking biological changes caused by artificial and natural factors and environmental impact assessment.

Retrospective study on survival and complication rates of posterior single implant according to the implant diameters, lengths and position (구치부 단일 임플란트의 직경과 길이, 식립 위치에 따른 생존율 및 합병증의 발생률에 대한 후향적 연구)

  • Soo-Young Hong;Seon-Ki Lee;Jin-Han Lee;Jae-In Lee
    • The Journal of Korean Academy of Prosthodontics
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    • v.61 no.2
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    • pp.101-112
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    • 2023
  • Purpose. This retrospective study was to investigate the survival and complication rates of posterior single implant according to the implant diameters, lengths and position. Materials and methods. Patients who had been restored single implant prosthesis in the posterior area by the three prosthodontists in the department of prosthodontics, Daejeon dental hospital of Wonkwang university, in the period from February 2014 to May 2018 were selected for the study. A total of 505 patients with 697 implants were observed. The survival and complications of implants were investigated using electronic medical records and radiographs. Fixture diameters, lengths, position, patient's sex and age were assessed as possible factor affecting the survival and complications of implants. Results. 3-year cumulative survival rates of posterior single implants were 98.5% and 5-year cumulative survival rates were 94.4%. 5-year cumulative survival rates were higher in implants with diameter > 4.0 mm (97.0%) than implants with diameter ≤ 4.0 mm (89.5%), and in females (98.8%) than males (92.4%). There were statistically significant differences (P < .05). The mechanical complication rate was 20.1% and the biological complication rate was 4.7%. Complications occurred in order of abutment screw loosening (7.5%), decementation (6.3%), proximal contact loss (3.7%) and so on. Abutment screw loosening occurred more frequently in the lower molar region (10.5%), in males (9.5%) than females (5.1%), and in patients aged < 65 years (9.4%) than patients aged ≥ 65 years (5.1%). There were statistically significant differences (P < .05). Conclusion. The 5-year cumulative survival rates were higher in implants with diameter > 4.0 mm than implants with diameter ≤ 4.0 mm and in females than males. Abutment screw loosening which was the most commonly occurring complication occurred more frequently in the lower molar region, in males than females, and in patients aged < 65 years than patients aged ≥ 65 years. There were statistically significant differences.

The effect of Rural Bus Service Quality Factors on Service Satisfaction and Policy Satisfaction: Focusing on Rural Bus Service in Jeollabuk-do (농어촌버스 서비스품질 요인이 서비스 만족도 및 정책 만족도에 미치는 영향: 전라북도 농어촌버스 서비스를 중심으로)

  • Kim, Jin Sung;Kim, Jae Geun
    • Journal of Service Research and Studies
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    • v.13 no.2
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    • pp.62-81
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    • 2023
  • The purpose of this study is to propose policies to improve and revitalize rural bus services in Jeollabuk-do in the future by identifying the factors that affect service satisfaction and policy satisfaction and the relationship between them, targeting rural bus users in 4 cities and counties in Jeollabuk-do. In the four cities and counties in the study area, the number of rural bus users is continuously decreasing, and various transportation policies, such as unification of bus fares and demand-response transportation, are being piloted to promote bus use. In this study, three major service quality factors that affect rural bus service satisfaction and policy satisfaction were derived after a literature review. Modifying the research model presented in previous studies, we have presented a research model to verify the relationship between the three factors affecting service satisfaction and policy satisfaction, and the relationship between service satisfaction and policy satisfaction. For the verification of the research model, a total of 520 survey data collected from passengers near bus stops and bus terminals in four cities and counties, Gochang, Gimje, Buan, and Jeongeup, were used. As a result of the verification, it was found that the three factors of economic feasibility, convenience, and safety had a statistically positive (+) effect on service satisfaction, but only convenience and safety had a positive (+) effect on policy satisfaction. Contrary to the research hypothesis, it was found that there was no statistically significant relationship between service satisfaction and policy satisfaction. Although the result is different from the research hypothesis, it was a meaningful result that can make policy suggestions on the improvement of the unified fare system policy, service satisfaction, and policy satisfaction to Jeollabuk-do rural bus transportation policy makers and working-level officers. In conclusion, based on the results of research analysis, the unified fare system, and transportation policy, measures to improve service satisfaction and policy satisfaction were presented.

The effect of the decision to use innovative services on the choice of consumers with a risk-averse tendency (혁신 서비스 이용 결정이 위험회피 성향 소비자의 선택에 미치는 영향)

  • Park, Kikyoung
    • Journal of Service Research and Studies
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    • v.13 no.2
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    • pp.146-160
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    • 2023
  • The spread of non-face-to-face services due to the COVID-19 pandemic has brought many changes in consumers' purchasing behavior and attracted much attention to new services. Could trying new services caused by this sudden environmental change alter consumers's choice patterns? This study proposes the research question of whether these new service experiences can change consumers' existing choice behavior, especially for risk-averse consumers who maintain their existing choice behavior or prefer safe alternatives. In this study, we examined whether trying out an unmanned payment services, one of innovative services that emerged after the pandemic crisis, can change the existing choice behavior of risk-averse consumers, i.e., make them more likely to prefer risky alternatives to safe alternatives. To accomplish these research goals, this research conducted one pilot survey and one study. The results of pilot survey showed that the stronger the prevention-focus tendency, the lower the self-efficacy to use the innovative service, with a negative relationship between them. Based on these findings, the study used an experimental method to examine the interaction effects between the use of innovation services and consumers' regulatory focus in a choice behavior and to explore the psychological mechanisms behind them. According to the results, it is found that prevention-focused consumers were more likely to choose risky alternatives and dissimilar extended brands following a trial of an unmanned payment service compared to not using that service. In contrast, promotion-focused consumers did not show different choice patterns regardless of following a trial of an innovative service. Furthermore, these results for prevention-focused consumers confirm the role of self-efficacy as a psychological mechanism. These findings shed light on the role of self-efficacy which has discussed in positive psychology into marketing area. Moreover, practical and academic implications are suggested by the finding that behavioral change occurs in risk-averse consumers, who are known to be hesitant to try new behaviors, indicating market expansion related to potential consumers for the use of the innovation services.

Evaluation of estuary reservoir management based on robust decision making considering water use-flood control-water quality under Climate Change (이수-치수-수질을 고려한 기후변화 대응 로버스트 기반 담수호 관리 평가)

  • Kim, Seokhyeon;Hwang, Soonho;Kim, Sinae;Lee, Hyunji;Kwak, Jihye;Kim, Jihye;Kang, Moonseong
    • Journal of Korea Water Resources Association
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    • v.56 no.6
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    • pp.419-429
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    • 2023
  • The objective of this study was to determine the management water level of an estuary reservoir considering three aspects: the water use, flood control and water quality, and to use a robust decision-making to consider uncertainty due to climate change. The watershed-reservoir linkage model was used to simulate changes in inflow due to climate change, and changes in reservoir water level and water quality. Five management level alternatives ranging from -1.7 El.m to 0.2 El.m were evaluated under the SSP1, 2, 3, and 5 scenariosof the ACCESS-CM2 Global Climate Model. Performance indicators based on period-reliability were calculated for robust decision-making considering the three aspects, and regret was used as a decision indicator to identify the alternatives with the minimum maximum regret. Flood control failure increased as the management level increased, while the probability of water use failure increased as the management level decreased. The highest number of failures occurred under the SSP5 scenario. In the water quality sector, the change in water quality was relatively small with an increase in the management level due to the increase in reservoir volume. Conversely, a decrease in the management level resulted in a more significant change in water quality. In the study area, the estuary reservoir was found to be problematic when the change in water quality was small, resulting in more failures.

Behaviors of Soft Bangkok Clay behind Diaphragm Wall Under Unloading Compression Triaxial Test (삼축압축 하에서 지중연속벽 주변 방콕 연약 점토의 거동)

  • Le, Nghia Trong;Teparaksa, Wanchai;Mitachi, Toshiyuki;Kawaguchi, Takayuki
    • Journal of the Korean Geotechnical Society
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    • v.23 no.9
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    • pp.5-16
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    • 2007
  • The simple linear elastic-perfectly plastic model with soil parameters $s_u,\;E_u$ and n of undrained condition is usually applied to predict the displacement of a constructed diaphragm wall(DW) on soft soils during excavation. However, the application of this soil model for finite element analysis could not interpret the continued increment of the lateral displacement of the DW for the large and deep excavation area both during the elapsed time without activity of excavation and after finishing excavation. To study the characteristic behaviors of soil behind the DW during the periods without excavation, a series of tests on soft Bangkok clay samples are simulated in the same manner as stress condition of soil elements happening behind diaphragm wall by triaxial tests. Three kinds of triaxial tests are carried out in this research: $K_0$ consolidated undrained compression($CK_0U_C$) and $K_0$ consolidated drained/undrained unloading compression with periodic decrement of horizontal pressure($CK_0DUC$ and $CK_0UUC$). The study shows that the shear strength of series $CK_0DUC$ tests is equal to the residual strength of $CK_0UC$ tests. The Young's modulus determined at each decrement step of the horizontal pressure of soil specimen on $CK_0DUC$ tests decreases with increase in the deviator stress. In addition, the slope of Critical State Line of both $CK_0UC$ and $CK_0DUC$ tests is equal. Moreover, the axial and radial strain rates of each decrement of horizontal pressure step of $CK_0DUC$ tests are established with the function of time, a slope of critical state line and a ratio of deviator and mean effective stress. This study shows that the results of the unloading compression triaxial tests can be used to predict the diaphragm wall deflection during excavation.

Numerical Analysis of Pile Foundation Considering the Thawing and Freezing Effects (융해-동결작용을 고려한 말뚝 기초에 관한 수치해석 연구)

  • Park, Woo-Jin ;Park, Dong-Su;Shin, Mun-Beom;Seo, Young-Kyo
    • Journal of the Korean Geotechnical Society
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    • v.39 no.5
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    • pp.51-63
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    • 2023
  • Numerical analysis was conducted to determine the effect of soil behavior by thawing and freezing of seasonal frozen soil on pile foundations. The analysis was performed using the finite element method (FEM) to simulate soil-pile interaction based on the atmosphere temperature change. Thermomechanical coupled modeling using FEM was applied with the temperature-dependent nonlinear properties of the frozen soil. The analysis model cases were applied to the MCR and HDP models to simulate the elastoplastic behavior of soil. The numerical analysis results were analyzed and compared with various conditions having different length and width sizes of the pile. The results of the numerical analysis showed t hat t he HDP model was relat ively passive, and t he aspect and magnit ude of t he bearing capacit y and displacement of the pile head were similar depending on the length and width of the pile conditions. The vertical displacement of the pile head by thawing and freezing of the ground showed a large variation in displacement for shorter length conditions. In the MCR model, the vertical displacement appeared in the maximum thaw settlement and frost heaving of 0.0387 and 0.0277 m, respectively. In the HDP model, the vertical displacement appeared in the maximum thaw settlement and frost heaving of 0.0367 and 0.0264 m, respectively. The results of the pile bearing capacity for the two elastoplastic models showed a larger difference in the width condition than the length condition of the pile, with a maximum of about 14.7% for the width L condition, a maximum of about 5.4% for M condition, and a maximum of about 5.3% for S condition. The significance of the effect on the displacement of the pile head and the bearing capacity depended on the pile-soil contact area, and the difference depended on the presence or absence of an active layer in the soil and its thickness.

Analysis of Effect on Pesticide Drift Reduction of Prevention Plants Using Spray Drift Tunnel (비산 챔버를 활용한 차단 식물의 비산 저감 효과 분석)

  • Jinseon Park;Se-Yeon Lee;Lak-Yeong Choi;Se-woon Hong
    • Journal of Bio-Environment Control
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    • v.32 no.2
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    • pp.106-114
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    • 2023
  • With rising concerns about pesticide spray drift by aerial application, this study attempt to evaluate aerodynamic property and collection efficiency of spray drift according to the leaf area index (LAI) of crop for preventing undesirable pesticide contamination by the spray-drift tunnel experiment. The collection efficiency of the plant with 'Low' LAI was measured at 16.13% at a wind speed of 1 m·s-1. As the wind speed increased to 2 m·s-1, the collection efficiency of plant with the same LAI level increased 1.80 times higher to 29.06%. For the 'Medium' level LAI, the collection efficiency was 24.42% and 43.06% at wind speed of 1 m·s-1 and 2 m·s-1, respectively. For the 'High' level LAI, it also increased 1.24 times higher as the wind speed increased. The measured results indicated that the collection of spray droplets by leaves were increased with LAI and wind speed. This also implied that dense leaves would have more advantages for preventing the drift of airborne spray droplets. Aerodynamic properties also tended to increase as the LAI increased, and the regression analysis of quadric equation and power law equation showed high explanatory of 0.96-0.99.

Leaf Mineral Contents and Growth Characteristics of Strawberry Grown in Aquaponic System with Different Growing Media in a Plant Factory (식물공장형 아쿠아포닉스 시스템에서 배지 종류에 따른 딸기 잎의 무기이온 함량과 생육 특성)

  • Su-Hyun Choi;Min-Kyung Kim;Young-Ae Jeong;Seo-A Yoon;Eun-Young Choi
    • Journal of Bio-Environment Control
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    • v.32 no.2
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    • pp.122-131
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    • 2023
  • This study was aimed to determine the effects of grow media on the mineral contents of the leaves and growth characteristics of strawberry grown under aquaponics system in a plant factory. For aquaculture, 12 fish (Cyprinus carpio) (total weight, 2.0 kg) were raised in an aquaponics tank (W 0.7 m × L 1.5 m × H 0.45 m, 472.5 L) filled with 367.5 L of water at a density of 5.44 kg·m-3 and total 34 of strawberry seedlings were transplanted in the pots filed with 200 g of orchid stone, hydroball or polyurethane sponge in the growing bed (W 0.7 m × L 1.5 m × H 0.22 m) laid out with holly acrylic sheet (140×60 mm, Ø80) on the top of the system. The pH and EC of the aquaponic solution was ranged from 7.6 to 4.9 and 0.24-0.91 dS·m-1, respectively. The concentration of NO3-N was about 28% lower than that of the hydroponic standard solution, and K, Fe and B were 10, 27 and 3.8 times lower, respectively; however, the mineral contents of strawberry leaves were in the appropriate ranges with lower contents in the leaves grown with sponge media. The organic content (OM), nitrogen (N), phosphorus (P), and potassium (K) of the sludge were 61.5, 5.72, 8.92, and 0.24%, respectively. The leaf area, leaf number, and dry and fresh weights of shoot at 81 DAT were significantly higher in the hydroball, and the average number of fruits per plant was significantly higher in both the orchid stone and hydroball. There was no significant difference in the fresh and dry weights of fruits. Integrated all the results suggest that the orchid stone and hydroball media are more effective to utilize nutrients in solid particles of aquaponic solution, compared to the polyurethane sponge.

The Maritime Geography of Korea Strait: Suggested Nomenclature and Cartographic Boundaries Derived from a Review of Historical and Contemporary Maps (국제학술지, 지도, 문서에 나타난 대한해협 해양지명과 경계에 대한 인식 변화)

  • DO-SEONG BYUN;BYOUNG-JU CHOI
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.28 no.2
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    • pp.63-93
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    • 2023
  • This study aims to examine the history of naming the strait between the Yellow and East China Seas and the East Sea to suggest a consistent nomenclature and to demarcate the geographic region of the strait. Although the strait is internationally known as 'Korea Strait', it is commonly referred to as the 'South Sea' in Korean common usage. This review ultimately recommends the use of 'Korea Strait' as an appropriate geographical name for this area. To support this recommendation, the historical boundaries typically assigned to the Korea Strait were investigated. We also analyzed the evolution of geographical labels assigned to Korea Strait and to the Western and Eastern Channels (labels given to the two maritime areas surrounding Tsushima). Resources for this analysis included historic maps and charts, International Hydrographic Organization Special Publications (S-23), and maps published in the Ocean Science Journal (OSJ) and Journal of Oceanography (JO), which are two international journals representing Korean and Japanese sources, respectively, from 2005 to 2021. In these two international journals, the most frequently used names assigned to the strait of interest were Korea Strait (appearing 42.9% of OSJ maps, and 7.5% of JO maps), and Tsushima Strait (appearing 60.4% of JO maps, and 0% of OSJ maps). Other names were South Sea and Korea Strait/Tsushima Strait. On maps in the two reviewed journals, the boundaries of Korea Strait were defined explicitly or implicitly in five different ways: a broad region between the Yellow and East China Seas and Ulleung Basin (Type 1), the region between Ulleung Basin and Tsushima (Type 2), the western channel of the strait (Type 3-1), the eastern channel of the strait (Type 3-2), and both the western and eastern channels of the strait (Type 4). Overall, Type 1 was the most frequently used boundary, taking up 71.4% of OSJ and 60.4% of JO maps. Lastly, we suggest in this paper that the current flowing through Korea Strait from the East China Sea to the East Sea should be labeled the 'Korea Strait Warm Current' to indicate its full path through the strait. Currently, this current is internationally referred to as the 'Tsushima Warm Current', which does not link well to the commonly used geographic name of the strait.