• 제목/요약/키워드: ANR1

검색결과 190건 처리시간 0.025초

Isolation of an Autonomously Replicating DNA Sequence from Aspergillus nidulans

  • Jang, Seung-Hwan;Jahng, Kwang-Yeon
    • Journal of Microbiology
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    • 제37권2호
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    • pp.51-58
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    • 1999
  • Using yeast, Saccharomyces cerevisiae, and the integrate vector system, we have isolated and characterized an autonomously replicating sequence (ARS) from Aspergillus nidulans. The DNA fragment, designated ANR1, is 5.0 kb in size and maintained free from the chromosome in S. cerevisiae. The YIplac211-ANR1 recombinant plasmid, which consists of sequences derived from the yeast integrative vector YIplac211 and 5.0 kb ANR1 fragment, showed a 104-fold enhancement in transformation efficiency over that found for YIplac211, and was easily recovered from the transformed yeast. Genetic analysis of transformants showed that YIplac21-ANR1 could be over 96% cured when cultured over 20 generations in complete medium and thus suggests that this sequence is mitotically unstable. In A. nidulans, recombinant plasmid PILJ16-4.5 which carries the 4.5 kb EcoRI fragment of ANR1 showed a 170-fold enhancement in transformation efficiency compared to that of the integrative vector PILJ16.

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DNA 표지를 이용한 딸기 국내 육성 품종 판별 (Identification of Korean Strawberry Cultivars using DNA markers)

  • 조강희;노일래;조용섭;박부희
    • 한국육종학회지
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    • 제40권4호
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    • pp.401-407
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    • 2008
  • 딸기 국내 육성 신품종을 정확히 판별할 수 있는 DNA표지를 개발하고자 실험을 수행하였다. 딸기 유전정보를 이용하여 품종 판별이 가능한 CAPS 표지 15종을 개발하였고, 그 중에서 6종은 품종 특이적인 표지였다. CAPS 표지 중에서 ANR-MspI, ANR-BamHI, ACO-HinfI, DFR-AseI, FGT-MspI의 최소 5종의 표지를 이용하여 '매향'과 '선홍'을 제외한 국내 육성 품종 판별이 가능하였다. 15종의 CAPS 표지를 보완하기 위해 SRAP 분석을 통해 품종 간 다형성을 나타내는 15종의 표지를 선발하였고, 그 중에서 me1/em5-460bp 표지를 이용하여 '매향'과 '선홍'의 구별이 가능하였다. 따라서 5종의 CAPS 표지와 1종의 SRAP 표지를 이용하여 19종의 국내 육종 품종과 일본 품종의 판별이 가능하였으며, 금후 이 연구결과는 딸기 국내 육성 품종 식별을 위해 효과적으로 이용될 수 있을 것으로 판단되었다.

Gottlieb groups of spherical orbit spaces and a fixed point theorem

  • Chun, Dae Shik;Choi, Kyu Hyuck;Pak, Jingyal
    • 대한수학회보
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    • 제33권2호
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    • pp.303-310
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    • 1996
  • The Gottlieb group of a compact connected ANR X, G(X), consists of all $\alpha \in \prod_{1}(X)$ such that there is an associated map $A : S^1 \times X \to X$ and a homotopy commutative diagram $$ S^1 \times X \longrightarrow^A X $$ $$incl \uparrow \nearrow \alpha \vee id $$ $$ S^1 \vee X $$.

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A new theoretical model for the dynamical analysis of Nano-Bio-Structures

  • Di Sia, Paolo
    • Advances in nano research
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    • 제1권1호
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    • pp.29-34
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    • 2013
  • The conversion of mechanical energy into electrical energy at nanoscale using piezoelectric nanowire arrays has been in detail shown by deflection of nanowires. Recently it has performed an analytical model, both at classical and at quantum level, for describing the most important quantities concerning transport phenomena; the model predicts interesting peculiarities, as high initial charge diffusion in nanodevices constituting by nanowires and permits also in particular to deduce interesting informations about the devices sensitivity, focusing on the correlation between sensitivity and high initial diffusivity of these materials at nanometric level.

Forced vibration analysis of cracked functionally graded microbeams

  • Akbas, Seref D.
    • Advances in nano research
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    • 제6권1호
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    • pp.39-55
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    • 2018
  • Forced vibration analysis of a cracked functionally graded microbeam is investigated by using modified couple stress theory with damping effect. Mechanical properties of the functionally graded beam change vary along the thickness direction. The crack is modelled with a rotational spring. The Kelvin-Voigt model is considered in the damping effect. In solution of the dynamic problem, finite element method is used within Timoshenko beam theory in the time domain. Influences of the geometry and material parameters on forced vibration responses of cracked functionally graded microbeams are presented.

Synthesis of polymer - based inverted opal and transformation of its optical properties

  • Masalov, Vladimir M.;Dolganov, Pavel V.;Sukhinina, Nadezhda S.;Dolganov, Vladimir K.;Emelchenko, Gennadi A.
    • Advances in nano research
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    • 제2권1호
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    • pp.69-76
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    • 2014
  • We have obtained opal-like photonic crystals based on opals and inverted opals exhibiting a shift of the selective reflection band toward longer and shorter wavelengths with respect to the diffraction band of the initial opal consisting of $SiO_2$ spheres. The contribution of frames forming three-dimensional periodic structures and that of fillers to the spectral arrangement of the diffraction bands has been determined.

Generation of valley polarized current in graphene using quantum adiabatic pumping

  • Wang, Jing;Chan, K.S.
    • Advances in nano research
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    • 제3권1호
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    • pp.39-47
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    • 2015
  • We study a device structure which can be used to generate pure valley current and valley polarized current using quantum adiabatic pumping. The design of the structure allows the flexibility of changing the structure from one for pure valley current generation to one for valley polarized current generation by changing the applied electric potentials through changing the symmetry of the structure. The device is useful for the development of valleytronic devices.