• 현장농수산연구지
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• 제25권4호
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• pp.138-147
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• 2024

본 연구는 국내 밀 43 품종에 대한 A. tumefaciens 형질 전환 효율을 검정하기 위해 GUS staining 분석을 수행한 것으로서 대부분의 밀 형질전환 연구가 특정 품종에 국한되어 있기 때문에 국내 장려 밀 품종에 대한 형질전환 효율에 영향을 미치는 조건에 대한 검정이 필요하다. 국내 밀 품종 중 32개에서 1개 이상의 조직에 염색된 신호가 관찰되었으며 4개 품종에서는 염색된 신호가 관찰되지 않았고 6개 품종에서 과도한 A. tumefaciens 성장이 관찰되었고, 7개 품종은 미성숙배의 크기 등의 이유로 GUS staining 분석이 불가능하여 추가 분석에서 제외하였다. 국내 밀 품종별 A. tumefaciens 감염 효율 비교 결과, 백립계 8개 품종 및 적립계 28개 품종에서는 평균적으로 유의한 차이가 없었다. 특히 조농밀, 조품밀, 새올밀, 조중밀, 새금강밀 등 적립계 품종에서 90% 이상의 높은 감염 효율이 관찰되었고, 전체 품종 중 22개는 50% 이상의 효율을 나타내었다. 본 연구를 통하여 조농밀 및 새올밀은 미성숙배에서 전체 조직에서 강한 GUS 발현으로 형질전환에 적합한 품종으로 나타났으며 금강 품종은 백립계에서 상대적으로 높은 발현을 보여 A. tumefaciens을 이용한 형질전환에 적합한 것으로 확인되었다. 추가적인 연구를 통해 품종의 조직배양 효율을 검정하고, 안정적인 GUS 발현 효율을 조사하는 것이 필요하며, 이를 통해 밀 형질전환에 이용 가능한 품종을 더욱 정밀하게 선발할 수 있을 것으로 판단된다. 해당 연구 결과는 국내 밀품종의 형질전환 효율을 높이기 위한 기초 자료로 활용 가능할 것이다.

• International Journal of Automotive Technology
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• 제8권3호
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• pp.383-394
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• 2007

Due to the clutch's non-linear dynamics, time-delays, external disturbance and parameter uncertainty, the automated clutch is difficult to control precisely during the launch process or automatic mechanical transmission (AMT) vehicles. In this paper, an enhanced fuzzy sliding mode controller (EFSMC) is proposed to control the automated clutch. The sliding and global stability conditions are formulated and analyzed in terms of the Lyapunov full quadratic form. The chattering phenomenon is handled by using a saturation function to replace the pure sign function and fuzzy logic adaptation system in the control law. To meet the real-time requirement of the automated clutch, the region-wise linear technology s adopted to reduce the fuzzy rules of the EFSMC. The simulation results have shown hat the proposed controller can achieve a higher performance with minimum reaching time and smooth control actions. In addition, our data also show that the controller is effective and robust to the parametric variation and external disturbance.

• Journal of Welding and Joining
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• 제34권5호
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• pp.19-25
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• 2016

Global warming is hot issue to keep the earth everlastingly. Despite the increase of the world population and the energy demand, the world oil supply and the oil price are hold the steady state. If we are not decrease the world population and the energy consumption, unforeseeable energy crisis will come in the immediate future. AMT acronym of Advanced Materials for Transportation is a non-profitable IEA-affiliated organization to mitigate the oil consumption and the environment contamination for the transportation. In recent, Annex X Multi-materials Joining was added to enhance the car body weight reduction cause the high fuel efficiency and the low emission of exhaust gas. Multi-materials are the advanced materials application technology to optimize the weight, the performance and the cost with the combination of different materials such as Al-alloy, Mg- alloy, AHSS and CFRP. In this study, the trends of AMT strategy and Al-alloy based multi-materials joining technology were review. Also several technologies for Al-alloy dissimilar joining were investigated.

• 한국지구물리탐사학회:학술대회논문집
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• 한국지구물리탐사학회 2003년도 Proceedings of the international symposium on the fusion technology
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• pp.335-339
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• 2003

The estimation of seawater intrusion into deep aquifers has been becoming an important subject in terms of site characterization for geological disposal of radioactive waste. Conventional direct-current resistivity methods have been used for ground water explorations and recently have been applied to environmental problems. However, electromagnetic methods are more practical and useful for such a deep investigation. We consider audio-frequency magnetotelluric (AMT) and surface-to-borehole electromagnetic (EM) tomography methods as promising tools for the investigation of deep aquifer. These methods were tested in the Hasunuma area, Chiba Prefecture, Japan. Although the study area is in an urban area, high-quality AMT data were acquired, which was mainly accomplished by night-time data recording and remote-reference data processing. One-dimensional inversion results of the AMT data revealed two extremely conductive zones, which is consistent with the electrical conductivity profile of pore water in core samples. It can be interpreted as the seawater intrusions into both zones. However, the chemical analysis of the groundwater sampled in the deep zone suggests that this groundwater must be fossil seawater that had been confined during sedimentation processes. In addition, the permeability coefficient of the deep layer is very low. Thus the deep conductive zone corresponds to the fossil seawater regarded as being difficult to flow.

• 한국자동차공학회논문집
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• 제7권5호
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• pp.96-106
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• 1999

In this study, the advanced shift control algorithm for parallel type hybrid drivetrain system with automated manual transmission(AMT) is proposed. The AMT can be easily realized by mounting the pneumatic actuators and sensors on the clutch and shift levers of the conventional manual transmission. By using the electronic-controlled AMT, engine and induction machine, it is possible to achieve the integrated control of overall system for the efficiency and the performance of the vehicle. Performing the speed control of the induction machine and the engine, the synchronization at gear shifting and the smooth engagement of clutch can be guaranteed. And it enables to reduce the shift shock and shorten the shift time. Hence, it results in the improvement of shift quality and the driving comfort of the vehicle. Dynamometer-based experiments are carried out to prove the validity of the proposed shift control algorithm.

• 산업경영시스템학회지
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• 제34권4호
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• pp.66-71
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• 2011

This article proposes a novel shift automation mechanism for an automated manual transmission (AMT). The development of an automated manual transmission is currently being paid considerable attention by vehicle manufacturers, with the prospects of combining the comfort of an automatic transmission and the high efficiency of a manual transmission. In order to automate the shift mechanism of a manual transmission, the proposed shift automation mechanism consists of two electric motors, cross shaped pinion gears, rack type shift rails, and a ball splined hollow shaft. First we describe the shift mechanism and operating principles of a manual transmission to investigate important design criteria for the shift automation device. And a new shift automation mechanism is described with its structure, elements, and operating principles in detail. Using a conventional manual transmission, we develop a full three-dimensional CAD model of an AMT which includes main components of the manual transmission and the designed shift automation mechanism. Finally we investigate the operating performances and feasibility of the designed AMT by a dynamic analysis.

• Journal of Information Technology Applications and Management
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• 제11권2호
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• pp.81-99
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• 2004

Manufacturing flexibility has strategic position in improving competitive advantage as a mechanism that responses environmental uncertainty. Thus this study aims at recognizing strategic meaning of manufacturing flexibility in enhancing the competitive advantage and measuring manufacturing flexibility and ultimately analyzing the effects of IT use on the manufacturing flexibility. Consequently we proved that IT use have an effect on manufacturing flexibility and manufacturing flexibility has an effect on competitive advantage in this study The results of this study are as follows. First, information acquisition and decision support by IT use has a significant effect on enhancing the manufacturing flexibility and manufacturing flexibility has a significant effect on improving the competitive advantage. Theses results are also means that IT use to support AMT usage is more important than AMT itself in the achieving manufacturing flexibility.

• 농업생명과학연구
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• 제45권1호
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• pp.119-124
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• 2011

Trichoderma spp.는 white biotechnology에서 이용되는 대표적인 미생물로서 이들이 강력하게 분비 생산하는 효소들은 산업적으로 매우 중요하다. 본 연구에서는 amylase, pectinase, cellobiohydrolase 및 xylanase 분비활성이 높은 것으로 밝혀진 Trichoderma sp. KACC 40541균주에 대한 Agrobacterium이용 형질전환을 수행하였으며 균주개량을 위한 효율적인 유전자도입 방법을 제시하였다. 특히 형질전환을 위하여서는 균사체에 대한 적정 농도의 NaOH처리가 매우 효과적임을 보여주었다.

• 생태와환경
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• 제53권2호
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• pp.195-207
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• 2020

난온대 동백나무군강의 한국 내 분포 북한계는 연평균 기온 13℃와 14℃, 한랭지수 -10℃로 제안된 바 있다. 본 연구는 이들 기후요소의 지리적 공간분포 실체를 검토하였다. 세 기후요소의 공간분포 양상은 티센망법과 크리깅법으로 분석하였으며, 세 가지 기후도를 생산하여 난온대 주요 상록활엽수종의 현존분포와 그 대응성을 비교·분석하였다. 결과로 난온대 식생의 분포 북한계는 한랭지수 -10℃가 연평균기온 14℃보다 일치하였으나, 연평균기온 13℃가 더욱 정교한 일치를 보여주었다. 한반도에서 연평균기온 13℃를 북한계로 한 난온대 식생역의 잠재 분포 면적은 20,334 ㎢로 다른 두 온도요소의 경우보다 넓었다. 본 연구는 가공하지 않은 직접 기후요소인 연평균기온 13℃가 가공으로부터 생겨난 간접 기후요소인 한랭지수 -10℃보다 난온대 식생 분포 북한계에 대응하는 특정 온도 요소인 것으로 밝혀졌다. 더불어 식물구계학적 특정종의 평가 기준으로 사용되는 난온대 경계선에 대한 학술적 재고의 필요성이 대두되었다.

• 한국자동차공학회논문집
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• 제12권4호
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• pp.119-128
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• 2004

With the growing traffic density and increasing comfort requirements, the automation of the drive train will gain importance in vehicles. The automatic clutch actuation relieves the drivers especially in urban driving and stop-and-go traffic conditions. This paper describes the dynamic modeling of a clutch actuator and clutch spring. The dynamic model of the clutch system is developed using MATLAB/Simulink, and evaluated by experimental data using a test rig. This performance simulator is useful to develop the clutch-by-wire (CBW) system for an automated manual transmission (AMT). The electro-mechanical type CBW system is also implemented as an automatic clutch for AMT. The prototype of CBW system is designed and implemented systematically, which is composed of an electric motor, worm gear and slider-crank mechanism. The test rig is developed to perform the basic function test of the automatic clutch, and the developed prototype is validated by the experimental data on the test rig.