• The Korean Journal of Mycology
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• v.44 no.2
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• pp.82-86
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• 2016

In this study, we investigated the spore diversity of arbuscular mycorrhizal fungi (AMF) in rhizospheres of a post-mining area and a natural forest area in Jecheon, Korea. The rhizospheres of the post-mining areas were dominated by Acaulospora mellea, while those of the natural forest area were dominated by Ambispora leptoticha. The number of AMF spores in rhizospheres of the post-mining area was significantly higher than that in the rhizospheres of the natural forest area. Although the diversity index of each area showed no significant difference, the community similarity of AMF within the rhizospheres of natural forest area was significantly higher than that observed within those of post-mining area. These results showed that AM fungal communities in rhizospheres could change because of the degree of disturbance.

• Journal of Microbiology and Biotechnology
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• v.25 no.7
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• pp.978-987
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• 2015

Aluminum (Al) toxicity is one of the greatest limitations to agriculture in acid soils, particularly in tropical regions. Arbuscular mycorrhizal fungi (AMF) can supply plants with nutrients and give protection against Al toxicity. The aim of this work was to evaluate the effects of soil liming (i.e., reducing Al saturation) on the AMF community composition and structure in the roots of maize lines contrasting for Al tolerance. To this end, we constructed four 18S rDNA cloning libraries from L3 (Al tolerant) and L22 (Al sensitive) maize lines grown in limed and non-limed soils. A total of 790 clones were sequenced, 69% belonging to the Glomeromycota phylum. The remaining sequences were from Ascomycota, which were more prominent in the limed soil, mainly in the L3 line. The most abundant AM fungal clones were related to the family Glomeraceae represented by the genera uncultured Glomus followed by Rhizophagus and Funneliformis. However, the most abundant operational taxonomic units with 27% of the Glomeromycota clones was affiliated to genus Racocetra. This genus was present in all the four libraries, but it was predominant in the non-limed soils, suggesting that Racocetra is tolerant to Al toxicity. Similarly, Acaulospora and Rhizophagus were also present mostly in both lines in non-limed soils. The community richness of AMF in the non-limed soils was higher than the limed soil for both lines. The results suggest that the soil Al saturation was the parameter that mostly influences the AMF species composition in the soils in this study.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.15 no.1
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• pp.45-51
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• 2005

This survey was performed to measure concentrations of airborne microbes, utilizing the six-stage cascade air sampler, according to the sampling site and time in the general hospital and to evaluate the degree of correlations between airborne microbes and environmental factors. Besides the main lobby, airborne microbes and environmental factors were not significantly different between the forenoon(9:00-10:00 AM) and afternoon(2:00-3:00 PM). Concentrations of airborne bacteria and fungi were 404 and $156CFU/m^3$ in the main lobby, 188 and $56CFU/m^3$ in the intensive care unit, 323 and $96CFU/m^3$ in the ward, 239 and $127CFU/m^3$ in the laboratory, and 139 and $33CFU/m^3$ in the newborn baby room, respectively. As a result, the level of airborne microbes was significantly highest in the main lobby and lowest in the newborn baby room(p<0.05). Outdoor airborne microbes concentrations were significantly lower than those of the sampling sites in the general hospital except for the newborn baby room(p>0.05). It was observed that temperature, relative humidity and carbon dioxide in the general hospital had generally positive correlation with airborne microbes. However, there was no correlation between the odor index level and airborne microbes.

• Journal of Korean Society of Forest Science
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• v.98 no.5
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• pp.539-547
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• 2009

This study was conducted to investigate the arbuscular mycorrhizal fungal biodiversity, spore density and root colonization in relation to site ages at expressway cut-slopes in Korea. Stabilization of exposed surface involved soil amendments and spraying seed mixture of turf grasses and/or nitrogen-fixing shrub species. Eighteen sites were selected with varying ages (2 to 16 years). Soil samples collected in October from each site were analyzed for fungal diversity and spore counts. Fine root samples from the plants were assayed for fungal colonization. Of the total 37 plants inspected in the sites, 26 species had endomycorrhizal colonization with an average root colonization rate of 18%, and with a range from 1 to 67%. The average endomycorrhizal colonization rate of initially introduced Festuca arundinacea which became the most dominant grass in later stage showed 22.8%, while that of Lespedeza bicolor which became the most dominant woody species were 21.6%. Naturally-invading Robinia pseudoacacia showed higher colonization rate in the old sites. Although site age did not show significant effects on fungal diversity, the root colonization rates of initially introduced plants decreased with the site aging, while those of invading plants increased with aging of the sites. The soil chemical properties, pH, N, and P contents, were negatively correlated with spore density, root colonization and endomycorrhizal species diversity. A total of forty arbuscular mycorrhizal fungal species in seven genera were identified. Of the 40 species, Acaulospora lacunosa, Glomus aggregatum, Glomus constrictum, Scutellospora erythropa, and Acaulospora spinosa were the five most dominant species in the decreasing order.

• Mycobiology
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• v.33 no.1
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• pp.51-60
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• 2005

Pot greenhouse experiments were carried out to attempt to increase the salinity tolerance of one of the most popular legume of the world; cowpea; by using dual inoculation of an Am fungus Glomus clarum and a nitrogen-fixer Azospirillum brasilense. The effect of these beneficial microbes, as single- or dual inoculation-treatments, was assessed in sterilized loamy sand soil at five NaCl levels ($0.0{\sim}7.\;2ds/m$) in irrigating water. The results of this study revealed that percentage of mycorrhizal infection, plant height, dry weight, nodule number, protein content, nitrogenase and phosphatase activities, as well as nutrient elements N, P, K, Ca, Mg were significantly decreased by increasing salinity level in non-mycorrhized plants in absence of NFB. Plants inoculated with NFB showed higher nodule numbers, protein content, nitrogen concentration and nitrogenase activities than those of non-inoculated at all salinity levels. Mycorrhized plants exhibited better improvement in all measurements than that of non-mycorrhized ones at all salinity levels, especially, in the presence of NFB. The concentration of $Na^+$ was significantly accumulated in cowpea plants by rising salinity except in shoots of mycorrhizal plants which had $K^+/Na^+$ ratios higher than other treatments. This study indicated that dual inoculation with Am fungi and N-fixer Azospirillum can support both needs for N and P, excess of NaCl and will be useful in terms of soil recovery in saline area.

• Mycobiology
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• v.39 no.2
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• pp.79-84
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• 2011

Nuclear distribution within the extra-radical fungal structures and during spore production in the arbuscular mycorrhizae fungus Glomus intraradices was examined using an in vitro monoxenic culture system. A di-compartmental monoxenic culture system was modified using a nitrocellulose membrane and a coverglass slip for detailed observations. Nuclear distribution was observed using the fluorescent DNA binding probes SYBR Green I and DAPI. Both septate and non-septate mycelial regions were observed, but cytoplasmic contents were only found within non-septate mycelia. Nuclear fluorescent staining revealed that the non-septate hyphal region contained nuclei only with cytoplasm, and that nuclear distribution was limited by septa. Swollen hyphal bodies were often associated with septate and empty-looking hyphae. Cytoplasmic contents filled the swollen hyphal body from the non-septate hyphal region following removal of the septa. As a consequence, the swollen body developed into a new spore. These observations provide understanding about the distribution of AM fungal nuclei within extra-radical mycelia and during spore formation. The results suggest a mechanism by which the development of a cytoplasm-containing mycelium is controlled by the formation or removal of septa to efficiently maintain and proliferate essential contents. This mechanism may provide a survival strategy to the fungus.

• Journal of Mushroom
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• v.18 no.1
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• pp.1-9
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• 2020

Mycorrhiza refers to the association between a plant and a fungus colonizing the cortical tissue of the plant's roots during periods of active plant growth. The benefits afforded by plants from mycorrhizal symbioses can be characterized either agronomically, based on increased growth and yield, or ecologically, based on improved fitness (i.e., reproductive ability). In either case, the benefit accrues primarily because mycorrhizal fungi form a critical linkage between plant roots and the soil. The soilborne or extramatrical hyphae take up nutrients from the soil solution and transport them to the root. This mycorrhizae-mediated mechanism increases the effective absorptive surface area of the plant. There are seven major types of mycorrhizae along with mycoheterotrophy: endomycorrhizae (arbuscular mycorrhizae, AM), ectomycorrhizae (EM), ectendomycorrhizae, monotropoid, arbutoid, orchid, and ericoid. Endomycorrhizal fungi form arbuscules or highly branched structures within root cortical cells, giving rise to arbuscular mycorrhiza, which may produce extensive extramatrical hyphae and significantly increase phosphorus inflow rates in the plants they colonize. Ectomycorrhizal fungi may produce large quantities of hyphae on the root and in the soil; these hyphae play a role in absorption and translocation of inorganic nutrients and water, and also release nutrients from litter layers by producing enzymes involved in mineralization of organic matters. Over 4,000 fungal species, primarily belonging to Basidiomycotina and to a lesser extent Ascomycotina, are able to form ectomycorrhizae. Many of these fungi produce various mushrooms on the forest floor that are traded at a high price. In this paper, we discuss the benefits, nutrient cycles, and artificial cultivation of mycorrhizae in Korea.

• Journal of The Korean Society of Grassland and Forage Science
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• v.18 no.3
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• pp.195-204
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• 1998

This study was designed to investigate the effects of antagonistic microorganism, Bacillus subtilis, on the growth of forage seedlings in repeated cultivation soils and unrepeated cultivation soils. The field experiment was wnducted in pots in a vinyl house using repeated and unrepeated cultivation soils. Forage types were 'Suwon 19' wrn(Zea mqs L.), 'Califbmia' white clover(Tr~oIium repens L.) and 'Fawn' tall fescue (Festuca arundianacea Schreb.). Samples of white clover and tall fescue were taken h m each pot at 36 days after seeding. Samples of wm were examined at 50 days after seeding. The most active antagonistic bacterium was isolated h m forage rhizosphere soil, and selected by reference to it's antagonistic ability on the growth of pathogenic fungi, Rhizoctonia solmi and Fusarium oxyspomm, and it was identified as Bacillus subtilis. This strain strongly suppressed the growth of fungal pathogens among isolated rhizobacteria. The dry weight of forage shoots and roots cultivated in unrepeated cultivation soils was higher than that cultivated in repeated cultivation soils. The dry weight of forage was positively affected by the inoculation of the antagonistic bacterium, Bacillus subtilis, in both repeated cultivation soils and unrepeated cultivation soils. In conclusion, the growth of forage was more affected by the inoculation of the antagonistic bacterium in unrepeated cultivation soils than that in repeated cultivation soils, and bacterization of forage with B. subtilis resulted in an inrreased yield.

• Journal of the Korean Society of Clothing and Textiles
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• v.20 no.1
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• pp.98-112
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• 1996

Tho purpose of this study was to investigate hygienic and comfortable properties of socks. Materials are nine summer socks either frequently being worn or new products recently introduced to market. Three female and three male adults participated in this study. Through wearing experiment, the numbers of microbes on foot and sock were counted and subjective sensation was measured. The microbes were isolated and identified based on growth physiological characteristics. Nine different socks had smaller number of bacteria of sock than that of foot. The number of bacteria of sock was significantly related with that of foot in cotton socks, in piled cotton socks, in mesh cotton socks, in cotton+ nylon+ linen blended socks, in nylon socks. Total number of bacteria of tv cut finished socks was most small and total number of bacteria was increased in the order of ultra fresh finished socks, untreated cotton socks, nylon socks, cotton + nylon+ linen blended socks, mesh cotton socks, polyester+ nylcn+ linen blended socks, piled cotton socks, cotton socks. Total number of bacteria of cotton socks and piled cotton socks were significantly different from that of uv cut finished socks. Finished socks and .jocks has high air permeability had significantly small number of bacteria. Comfortable sensation in nylon socks and polyester+nylon+linen socks was significantly uncomfortable. The way socks finished and air permeability of .jocks affected theirs hygienic property, while fiber type of them affected comfortablene, is. Bacteria identified were Staphylo coccus aureus, S. au rice larir, S. cahn ii, S. ep ids midis, S. haemo Iyticus, S. h am in 2's. S.fapraphyticus, S. warnery, 1 cinetobater calcoaceticus bio. anitratus, p.reudomonas mendocina, p. paucimobilis, Flavimonas Q ryzihabitans (CDC Group VE-2), and Xanthomanas maltophina. Fungi isolated were Spicaria sp., Thrichoderma sp., Fusarium sp., Aspergillus sp., Epicoccum sp., Cladosporium sp., and Penicillium sp..

• Korean Journal of Pharmacognosy
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• v.7 no.3
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• pp.199-208
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• 1976

저자(著者)등은 1976년(年) 7월(月) 17일(日)부터 동년(同年) 11월(月) 14일(日)까지의 기간(期間)동안 서울과 경기도내(內) 5개지역(서울 : 관악산, 경기도 : 수원, 광릉, 갈매리, 오산리)으로부터 100여 종(種)의 한국산(韓國産) 고등균류(高等菌類)를 채집하였으며, 이중 31종(種)을 분류(分類) 확인(確認)하였다. 분류(分類)된 31종중(種中) 9종(種) 즉 (1) Scutellinia scutellata $(St.\;A_{MANS})$ $L_{AM}$ (2) Peziza vesiculsa $F_{R}.$ (3) Helvella atra $F_{R}.$ (4) Neobulgaria pura $(P_{ERS}.)$ $P_{ETRAK}$ (5) Trametes coccinea $F_{R}.$ (6) Amanita melleiceps $H_{ONGO}$ (7) Cotylidia burtina $(P_{T}.)$ $I_{MAZ}.$ (8) Sarcodontia copelandii $(P_{AT}.)$ $I_{MAZ}.$ comb nov. (9) Sphaerobolus stellatus $P_{ERS}.$은 한국 미기록종(種)이며, 이외에 2종(種) (1) Lycoperdon D.M.C-2 및 (2) Tuber melanosporum도 잠정적으로 미기록종(種)이라 사료된다. 한편 Neobulgaria pira $P_{ERS}.$가 속해있는 과(科)인 Helotiaceae와 Sphaerobolus stellatus $P_{ERS}.$가 속해있는 과(科)인 Sphaerobolaceae는 각각(各各) 한국 미기록과(科)이다. 이에 저자등은 이들에게 신칭을 붙여 각각(各各)의 외부(外部)형태와 현미경적 특징에 대하여 기술하고, 이들의 사진과 함께 보고 하는 바이다.