• Journal of the Korean Geographical Society
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• v.33 no.2
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• pp.191-208
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• 1998

제주도의 기온온난화가 한라산 고산식물 분포에 미치는 영향에 대한 연구의 하나로 한라산 고산식물 8종의 수평 및 수직 분포역과 생육환경이 조사되었다. 그 결과 희귀하거나 멸종 위기 상태에 있는 고산식물들에 관한 생물지리학적 기초 자료와 정보가 축적되었다. 한라산 고산식물은 분포특성에 따라 8가지 유형으로 분류되며, 그 중 70퍼센트 정도가 한반도의 고산지나 일본열도의 것들과 공통적인 것으로 밝혀졌다. 이들은 빙하기 동안 동북아시아에서 한반도를 거쳐 제주도로 유입되었고, 일부는 일본열도에 전파된 것으로 생각된다. 현재 이들 고산식물은 열악한 기후, 척박한 토질과 지질 그리고 동결과 융해가 반복되는 주빙하성 환경에 적응하여 산정부 일대에 주로 분포한다. 본 연구는 한라산 고산식물의 분포와 과거 및 현재 환경과의 관계에 대한 기초 자료를 구축하여, 한반도의 자연환경 변천사를 파악하고, 기온온난화가 지속될 때 한라산 고산경관과 생태계가 당면할 문제를 파악하고 대안을 제시하는데 필요한 정보를 제공할 것으로 기대된다.

• The Plant Pathology Journal
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• v.35 no.4
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• pp.389-392
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• 2019

The studies on detection of the Strawberry mottle virus (SMoV) have been conducted in Poland for breeding programme purpose and for producers of strawberry plant material. Leaf samples collected from infected strawberry plants were grafted on Fragaria sp. Indicators which were maintained in greenhouse for further study. Seven Fragaria vesca var. semperflorens 'Alpine' indicators infected by SMoV were used for the study aimed on molecular characterization of virus isolates. Partial RNA2 was amplified from total nucleic acids using the RT-PCR method. The obtained amplicons separately digested with BfaI, FauI, HaeIII, HincI, and TaqI enzymes showed different restriction profiles. The nucleotide sequences analysis of RNA2 fragment confirmed the genetic diversity of the SMoV isolates as their similarity ranged from 94.7 to 100%. Polish isolates shared 75.7-99.2% identity with sequence of the virus strains from the Czech Republic, the Netherlands, and Canada. Phylogenetic analysis resulted in grouping of the isolates found in Poland together with one of the Czech strain whereas two other from the Czech and the strains from the Netherlands and Canada created the separate cluster.

• Korean Journal of Plant Resources
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• v.15 no.1
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• pp.50-56
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• 2002

This study was carried out to optimize the fertigation method using fermented swine liquid manure for the growth of two western vegetables, broccoli and celery. Plants were grown in a rain-shelter house and fertilized with a range of dilutions(efflux 5 dilution=Ef. 5, efflux 10 dilution=Ef. 10, efflux 25 dilution=Ef. 25, and efflux 50 dilution=Ef. 50) of the liquid manure or with conventional application of N : P$_2$O$\_$5/ : K$_2$O = 200 : 70 : 500kg/ha for broccoli, 250 : 210 : 240 kg/ha for celery as controls. After harvest, soil pH and K content decreased after using a high concentration of the liquid manure, Ef. 5, than after treatment with weaker concentrations at Ef. 25 and Ef. 50. On the other hand, soil electrical conductivity, content of P$_2$O$\_$5/, organic matter, total nitrogen, and NO$_3$-N at Ef. 5 increased as concentration of swine liquid manure increased. After harvest, available P$_2$O$\_$5/ in plant tissue did not differ significantly between any of the treatments. In broccoli, the lower concentration (Ef. 50) of swine liquid manure increased flowering over the other treatments, perhaps because the level of absorption into the plants is higher with lower concentration. The amounts of K and Ca in plant tissue were greatest after Ef. 25 and Ef. 50 treatments. Plant growth was best at Ef. 50 in broccoli, head height, head width, and head weight were the best with Ef. 25 and Ef. 50 treatments after harvest. In celery, leaf length was greater after Ef. 25 and Ef. 50 treatments than any other treatments. Total yield of celery of Ef. 25 and Ef. 50 treatments was twice that of conventional cultivation. On the other hand, yield severely decreased after application of high-concentration treatment at Ef. 5. In conclusion, fertigation of swine liquid manure, diluted in the range of Ef. 25 to Ef. 50, could improve yield and quality in broccoli and celery.

• Horticultural Science & Technology
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• v.19 no.3
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• pp.329-333
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• 2001

The study was performed to investigate the effect of a plug culture method in the early stage of a potato breeding program. The 105, 200 and 288-cell plug trays were used for raising potato seedlings. The % germination in plug trays ranged between 94 and 96%. Percent tuberization measured at 50 and 60, and 70 days after sowing was the greatest in 105-cell and 200-cell trays, respectively. However, tuberization was most delayed in 288-cell trays. Thus, it is considered that 105-cell tray is the most effective for the selection of early maturing plants. Plant maturity was determined by the morphological characteristics at the seedling stage of stolons cultured in plug trays. Most of seedlings with shorter stolons at seedling stage (1st generation) were the early-maturing, while most of seedlings with longer stolons were the late-maturing at the clonal stage (2nd generation). These results mean that the plant maturity in the early potato breeding program can be effectively grouped by observing the morphological characteristics of stolons after plug culture.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.11-11
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• 2020

Background : The genus Cypripediums are typically adapted to the low temperature and cold climates of the high land in Korea. The species which were reported in Korean peninsula are C.japonicum, C.macranthos, C.calceolus and C.guttatum. We had already reported the successful germination of the C.macranthos and C.guttatum. The small spotted lady's slipper orchid(C.guttatum) is most endangered plants to extinction of which population was reported less than 100 in Korea. The objective of the present research is restoration of the C.guttatum in ex situ and adaptation in high land and nursery. Methods and Results : The 350 seedlings of C.guttatum were transplanted in soil at alpine area(altitude 750m) and the nursery of Korea National Arboretum(KNA) in YangPyeong city on May 2019 and 2020 in order to verify the possibility of ex situ restoration. The sprouted ratio of the seedlings were less than 10% in the alpine area and 83% in the nursery of KNA. The reasons of the diverse survival ratio are presumed as the low moisture content in the soil and heavy shade of the alpine area. The 15 cypripedium species and 29 hybrids were also effectively adapted in the KNA rare plant garden. Conclusion : These results demonstrated the difficulties of the restoration for small spotted lady's slipper orchid in the natural habitation. From the above results, we may conclude that the critical factors of the genus Cypripedium are intensive care for the fragile seedlings during the 1^st year acclimatization.

• Korean Journal of Pharmacognosy
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• v.38 no.2 s.149
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• pp.187-191
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• 2007

The extraction yield of the methanolic extracts and 19${\alpha}$-hydroxyursane-type triterpenoid (19${\alpha}$-HUT) fraction were investigated in the unripe and ripe fruits and the leaves of the four Rubus plants (Rubus coreanus, R. crataegifolius, R. phoenicolasius, R. pungens var. oldhami) to develop the biomaterial 19${\alpha}$-HUT mixture as functional foods. Thin layer chromatogaphy-Densitometer (TLC-DM) was used to analyze the individual quantity of 19${\alpha}$-HUTs using standard compounds (euscaphic acid, tormentic acid, 23-hydroxytormentic acid, kaji-ichigoside F$_1$, rosamultin, niga-ichigoside F$_1$). The content of methanolic extract of the fruits were higher in the ripe stage than in the unripe stage whereas the content of 19${\alpha}$-HUT mixture varied with each Rubus species. The Rubus plants containing the highest amount of 19${\alpha}$-HUTs in the leaves were R. coreanus, R. phoenicolasius and R. pungens var. oldhami while only R. cratagefolius showed the highest content in the ripe fruits. The mean of total genin content of 19${\alpha}$-HUTs was 0.94 mg/g; that of the glycosides was 0.60 mg/g. The genin quantity was found in the order of 23-hydroxytormentic acid> euscaphic acid> tormentic acid; the glycoside was observed in the order of niga-ichig-oside F$_1$> kaji-ichigoside F$_1$> rosmaultin, by which the biosynthetic pathway of 23-hydroxytormentic acid and its glucoside niga-ichigoside F$_1$ via the intermediates tormentic acid and/or rosamultin was presumed. It is also suggested that the ripe fruits of R. crataegifolius will be desirable to use as functional foods rather than unripe fruits.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.9 no.5
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• pp.32-40
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• 2006

This study's objects are to suggest effective forest community-level management measures by identifying the vulnerable forest vegetation communities types to climate change through a comparative analysis with present forest communities identified and delineated in the Actual Vegetation Map. The methods of this study are to classify the climatic life zones based on the correlative climate-vegetation relationship for each forest vegetation community, the Holdridge Bio-Climate Model was employed. This study confirms relationship between forest vegetation and environmental factors using Pearson's correlation coefficient analysis. Then, the future distribution of forest vegetation are predicted derived factors and present distribution of vegetation by utilizing the multinomial logit model. The vulnerability of forest to climate change was evaluated by identifying the forest community shifts slower than the average velocity of forest moving (VFM) for woody plants, which is assumed to be 0.25 kilometers per year. The major findings in this study are as follows : First, the result of correlative analysis shows that summer precipitation, mean temperature of the coldest month, elevation, soil organic matter contents, and soil acidity (pH) are highly influencing factors to the distribution of forest vegetation. Secondly, the result of the vulnerability assessment employing the assumed velocity of forest moving for woody plants (0.25kmjyear) shows that 54.82% of the forest turned out to be vulnerable to climate change. The sub-alpine vegetations in regions around Mount Jiri and Mount Seorak are predicted to shift the dominance toward Quercus mongolica and Pinus densiflora communities. In the identified vulnerable areas centering the southern and eastern coastal regions, about 8.27% of the Pinus densiflora communities is likely to shift to sub-tropical forest communities, and 3.38% of the Quercus mongolica communities is likely to shift toward Quercus acutissima communities. In the vulnerable areas scattered throughout the country, about 8.84% of the Quercus mongolica communities is likely to shift toward Pinus densiflora communities due to the effects of climate change. The study findings concluded that challenges associated with predicting the future climate using RCM and the assessment of the future vulnerabilities of forest vegetations to climate change are significant.

• The Korean Journal of Pesticide Science
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• v.4 no.2
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• pp.37-43
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• 2000

Effects of silicon application on development of colonies of Sphaerotheca fuliginea were examined. Cucumber plants were applied with nutrient solutions amended with different concentrations of soluble silicon and selected leaves were inoculated with known concentrations of conidia of the pathogen. Colony number per leaf, colony area per leaf, and germination rate of conidia of S. fuliginea collected from the inoculated leaves were reduced as silicate concentrations in the nutrient solutions increased from 0.05 to 4.10 mM. The increase in resistance of plants to mildew infection was apparently due to silicate accumulation in leaves, and there was no correlation between cation or ionic strength effects and the silicate treatments. Silicate treatment in growth medium remarkably suppressed powdery mildew development on cucumber. Colonies of mildew fungus were visible with over approx. 38.3% of the mature leaf surface, while that of the leaves in high Si plants was 2.3% observed at 51 days after transplanting. No significant differences were observed between 1.7 mM and 3.4mM silicate treatments. Conidial germination rates were significantly reduced by increasing Si amendments. Conidial germination ranged from 14.7 to 20.3% for plants grown in low Si solution(<1.40 mM), and from 9.0 to 12.4% for plants grown in high Si solution(>1.8 mM). Foliar applications of Si with ${\geq}$ 17.0 mM decreased the number of powdery mildew colonies. Persistence of Si foliar sprays effects on cucumber demonstrated that the 17 mM Si spray applied 4 days before inoculation with S. fuliginea reduced mildew colony formation. The relationship was positive and linear.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.1
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• pp.61-67
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• 2010

The chemical components in plant cuticular wax can be used as markers to estimate the species composition of the diet of grazing animals. In this experiment, composition of the diet of yak on the Qinghai-Tibetan plateau was estimated using n-alkane analysis. During the grazing period, samples of whole plants of the species present, plus fecal samples voided by the yak, were collected, air-dried and ground prior to the extraction of cuticular wax n-alkanes. The species composition of the yak diets was estimated by relating fecal alkane contents to those of the plant species, using the 'ATWHAT'software package. The results showed that the n-alkane technique can detect the main dietary components selected by yak. The diet consumed by yak contained 33% Kobresia humilis, 67% Stipa aliena in summer pasture; 26% Potentilla anserine, 74% Carex qinghaiensis in autumn pasture; 52% Carex qinghaiensis, 32% Heteropappus bowerii and 16% Saussurea semifasciata in winter pasture and 5% Carex qinghaiensis, 95% Achnatherum splendens in spring pasture. The apparent selection for forbs is likely to be a reason for nutritional constraint of yak inhabiting alpine environments.

• Korean Journal Plant Pathology
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• v.14 no.5
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• pp.425-432
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• 1998

Gentian plants (Gentiana spp.) showing yellow ringspot, mosaic, necrotic fleck and malformation were collected from their growing areas in Taegu, Kyungpook province and Alpine Agricultural Experiment Station, Korea. Three viruses isolated from the naturally infected gentian were identified as broad bean wilt virus (BBWV), cucumber mosaic virus (CMV) and clover yellow vein virus identified as broad bean wilt virus (CIYVV) by their host range, immunosorbent electron microscopy (ISEM) and electron microscopy. Electron microscopic examination of negatively stained preparations showed that BBWV and CMV are spherical particles of 28 nm and 30 nm in diameter, and CIYVV is filamentous particles of ca. 780 nm in length. By ISEM, BBWV was detected mainly in gentian showing yellow ringspot and mottle, CIYVV in necrotic fleck leaf of gentian and CMV in narrow and distorted mosaic leaf of gentian. BBWV and CMV are the most prevalent in the cultivated gentian. In ultrathin sections of BBWV infected tissues, large aggregates of crystalline array of virus particles and vesicular body were found in the cytoplasm and vacuole of mesophyll cells. In case of CIYVV, pinwheel- and laminated aggregate-type inclusions as well as filamentous virus particles were observed in the cytoplasm of mesophyll cells.