• Korean Journal of Environmental Biology
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• v.32 no.1
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• pp.58-67
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• 2014

The outdoor mass cultivation is not possible for microalgae in Korea all year round, due to cold winter season. It is not easy to maintain proper level of productivity of microalgae even in winter. To prevent a drastic decrease of temperature in a greenhouse, two layers were covered additionally, inside the original plastic layer of the greenhouse. The middle layer was made up of plastic and the inner layer, of non-woven fabric. Acrylic transparent bioreactors were constructed to get more sunlight, not only from the upper side but also from the lateral and bottom directions. In winter at freezing temperatures, six different culture conditions were compared in the triply covered, insulated greenhouse. Wastewater after anaerobic digestion was used for the cultivation of microalgae to minimize the production cost. Water temperature in the bioreactors remained above $10^{\circ}C$ on average, even without any external heating system, proving that the triple-layered greenhouse is effective in keeping heat. Algal biomass reached to 0.37g $L^{-1}$ with the highest temperature, in the experimental group of light-reflection board at the bottom, with nitrogen and phosphorus removal rate of 92% and 99%, respectively. When fatty acid composition was analyzed using gas-chromatography, linoleate (C18 : 3n3) occupied the highest proportion up to 61%, in the all experiment groups. Chemical oxygen demand (COD), however, did not decrease during the cultivation, but rather increased. Although the algal biomass productivity was not comparable to warm seasons, it was possible to maintain water temperature for algae cultivation even in the coldest season, at the minimum cost.

• Weed & Turfgrass Science
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• v.2 no.2
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• pp.176-183
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• 2013

To explore hydrolysis methods for the efficient manufacture of sugar solutions from the freshwater alga Water-net (Hydrodictyon reticulatum, HR), acid hydrolysis, enzymatic hydrolysis, and combined hydrolysis (acid followed by enzymatic hydrolysis) were investigated. In the one-step acid hydrolysis, the reaction of 8% solids content using 2% sulfuric acid at $120^{\circ}C$ for 1 hour was desirable. In this case, glucose 27.44 g 100 g $DM^{-1}$ could be obtained from the HR-d13 samples. In the two-step acid hydrolysis, the primary hydrolysis (HR powder : 72% sulfuric acid = 1 g : 1.5 mL) was carried out for 1 hour at $60^{\circ}C$, and then the secondary hydrolysis was done for 1 hour at $120^{\circ}C$ after addition of distilled water 23.5 mL. In this case, glucose 35.11 g/100 g DM could be obtained from the HR-d13 samples. In the combined hydrolysis, 25% solids content using 2% hydrochloric acid were reacted for 1 hour at $120^{\circ}C$, and then citrate buffer and hydrolysis enzyme complexes (E1 1.0 mL+E2 0.2 mL $g^{-1}$ dried matter) were added and reacted for 1 - 2 days at $50^{\circ}C$. In this case, glucose 33.5 g 100 g $DM^{-1}$ could be obtained from the HR-d23+26 samples. In conclusion, combined hydrolysis was likely to be more useful saccharification method of HR biomass at a practical level, considering the glucose productivity, generation of fermentation-inhibiting substances (hydroxyl methyl furfural, furfural), and limited use of strong acid.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.19 no.4
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• pp.287-301
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• 2014

In order to understand environment condition and phytoplankton community before and after coastal upwelling, the influences of upwelling events on phytoplankton community were studied at 18 stations located the Southern part of East Sea, Korea from May to August 2013. The surface water masses showed low temperature and high salinity due to upwelling events at coastal stations (A1, B1, C1). Correlation between temperature and nutrients (DIP, r=-0.218, p<0.01; DIN, r=-0.306, p<0.01; silicate, r=-0.274, p<0.01) was significantly negative. This result could be explained that nutrients were supplied to surface water by the upwelling of bottom water. Phytoplankton communities were composed of 186 species. Phytoplankton abundance were relatively high in May (C1, $726{\times}10^3cells\;L^{-1}$) and July (A1, $539{\times}10^3cells\;L^{-1}$). Total chlorophyll a and micro-size fraction ($&gt;20{\mu}m$) increased at coastal stations in July and August, while phytoplankton abundance and total chl. a was much low in June. Dominant species in June was Pseudo-nitzschia spp. of which the cell size was $309{\mu}m^3$. Cell size of Pseudo-nitzschia spp. was smaller than dominant species in other period. Therefore, the increase in total chloro-phyll a and the size of phytoplankton was resulted in the sufficient supply of nutrients. In contrast, these tendencies were not observed at outside stations. These results suggested that coastal upwelling was an important influencing factor to determine the species composition and standing stock of phytoplankton community in the coastal waters of the Southern part of East Sea, Korea.

• Korean Journal of Environment and Ecology
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• v.33 no.5
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• pp.605-619
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• 2019

The objective of this study was to analyze eutrophication characteristics, empirical model analysis, and variation of water quality according to monsoon intensity in Hapcheon Reservoir for 16 years from 2002 to 2017. Long-term annual water quality analysis showed that Hapcheon Reservoir was in a meso-nutrition to eutrophic condition, and the eutrophic state intensified after the summer monsoon. Annual rainfall volume (high vs. low rainfall) and the seasonal intensity in each year were the key factors that regulate the long-term water quality variation provided that there is no significant change of the point- and non-point source in the watershed. Dry years and wet years showed significant differences in the concentrations of TP, TN, BOD, and conductivity, indicating that precipitation had the most direct influence on nutrients and organic matter dynamics. Nutrient indicators (TP, TN), organic pollution indicators (BOD, COD), total suspended solids, and chlorophyll-a (Chl-a), which was an estimator of primary productivity, had significant positive relations (p<0.05) with precipitation. The Chl-a concentration, which is an indicator of green algae, was highly correlated with TP, TN, and BOD, which differed from other lakes that showed the lower Chl-a concentration when nutrients increased excessively. Empirical model analysis of log-transformed TN, TP, and Chl-a indicated that the Chl-a concentration was linearly regulated by phosphorus concentration, but not by nitrogen concentration. Spatial regression analysis of the riverine, transition, and lacustrine zones of $log_{10}TN$, $log_{10}TP$, and $log_{10}CHL$ showed that TN and Chl-a had significant relations (p<0.005) while TN and Chl-a had p > 0.05, indicating that phosphorus had a key role in the algal growth. Moreover, the higher correlation of both $log_{10}TP$ and $log_{10}TN$ to $log_{10}CHL$ in the riverine zone than the lacustrine zone indicated that there was little impact of inorganic suspended solids on the light limitation in the riverine zone.

• Asian Journal of Turfgrass Science
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• v.20 no.2
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• pp.147-156
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• 2006

This study was initiated to evaluate the growth characteristics of seven creeping bentgrass cultivars in summer, 'Penncross' showed the worst visual quality, whereas 'Penn A-4' and 'Crenshaw' the best quality. 'Putter', which was maintained a fair quality during the test period, was regarded as a good cultivar because of no significant variation in summer as compared to the other caltivars. 'Crenshaw',' L-93' and 'Penn A-4' were greater in chlorophyll content and 'Penncross' lowest during the summer. Also, 'SR1020' had a low content of chlorophyll. 'Putter' greatly increased in chlorophyll content after fertilization. The highest shoot density($19.3/cm^2$) was found with 'L-93' in early August, followed by 'Crenshaw', 'Penn A-4', 'Putter', 'Dominant', and 'SR1020' in that order. However, 'Penncross' was lowest($15.7/cm^2$). As for a root length, 'L-93' was longest, being over an average 5.5cm. 'Penn A-4' and 'Putter' also showed good result in root growth. However, the root length considerably decreased with 'SR1020', 'Penncross' and 'Dominant' in summer. Brown patch was a serious disease for the most cultivars, except 'Penncross'. 'Dominant' had the most serious damage. 'Putter', 'L-93', 'Crenshaw', 'SR1020', and 'Penn A-4' were also greater in damage over the others. In regards of algae occurrence in summer, 'Penn A-4' had the least damage, while 'Dominant' the greatest. In conclusion, 'Crenshaw', 'Penn A-4' and 'L-93' were the best cultivars in terms of summer growth. Conversely, 'Penncross' was the poorest one. However, this study was conducted under the conditions of one-year old green. Accordingly, in-depth experiment should be done over several years to elucidate the characteristics of growth for the wide range of creeping bentgrass cultivars during the summer.

• Korean Journal of Environment and Ecology
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• v.33 no.2
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• pp.187-201
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• 2019

This study examined the physicochemical water quality and evaluated the ecological health in 14 sites of Geum River (upstream, mid-stream, and downstream) using the fish community distribution and guilds and eight multi-variable matrices of FAI (Fish Assessment Index) during June 2008-May 2009. The analysis of the water quality variables showed no significant variation in the upstream and mid-stream but a sharp variation due to the accumulation of organic matter from the point where the treated water of Gap and Miho streams flew. The analysis of physicochemical water properties showed that BOD, COD, TN, TP, Cond, and Chl-a tended to increase while DO decreased to cause eutrophication and algae development from the downstream where Miho and Gap stream merged. The analysis of fish community showed that the species richness index and species diversity index increased in the mid-stream area but decreased in the downstream area, indicating the stable ecosystem in the upper stream and the relatively unstable ecosystem in the downstream. The analysis of the species distribution showed that the dominant species were Zacco platypus that accounted for 20.9% of all fish species and Zacco koreanus that accounted for 13.1%. The analysis of the fish tolerance and feeding guild characteristics showed that the sensitive species, the insectivore species, and the aquatic species were dominant in the mid-stream point. On the other hand, contaminants from the sewage water treatment plant of Miho stream had a profound effect in the downstream to show the dominance of tolerant species, omnivorous species, and lentic species. Therefore, it is necessary to improve water quality by reducing the load of urban pollutants and to pay attention to the conservation and restoration of aquatic ecosystems.

• Food Science and Industry
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• v.51 no.4
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• pp.278-301
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• 2018

Although more than 80% of living organisms are found in marine ecosystems, only less than 10% of marine resources have been utilized for human food consumptions and other usages. It is well known that marine resources (fish, shellfish and algae) have exceptional nutritional properties; however, their functional characteristic has not been completely discovered. It is believed that metabolites (organic compounds, proteins, peptides, lipids, minerals, etc.) play an important role to show its biological properties. Marine proteins and peptides are considered to be future drugs due to their excellent biological activities with a fewer adverse side effect. Marine peptides show several biological activities, including antimicrobial, antioxidant, anti-inflammatory, anti-cancer, anti-viral, anti-tumor, anti-diabetic, anti-hypertensive, anti-coagulant, immunomodulatory, appetite suppressing and neuroprotective effects. Therefore, the pharmaceutical, nutraceutical, and cosmeceutical companies have been paid attention to the marine peptides to commercialize into products. This current review mainly focused on the above mentioned biological activities of marine peptides and protein hydrolysates as a functional food and pharmaceutical applications. To commercialize these materials in industrial level required large quantity in high-purity level, and it is complicated to produce huge quantity from the marine resources due to insufficient raw materials, unavailability of raw materials through a year, hinder the growth with geographical variations, and availability of compounds in extreme small quantities. The best solution for these issues is to introduce new modern technologies such as artificial intelligence robots, drones, submersibles and automated raw material harvesting vessels in farming industries instead of man power, which will lead to 4th industrial revolution.

• Journal of Environmental Impact Assessment
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• v.27 no.6
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• pp.548-561
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• 2018

The objectives of this study were to construct a three-dimensional (3D) hydrodynamic and water quality model (EFDC) for the river reach between the Daecheong dam and the Sejong weir, which are directly affected by Gap and Miho streams located in the middle of the Geum River, and to evaluate the trophic status and water quality improvement effect according to the flow control and pollutant load reduction scenarios. The EFDC model was calibrated with the field data including waterlevel, temperature and water quality collected from September, 2012 to April, 2013. The model showed a good agreement with the field data and adequately replicated the spatial and temporal variations of water surface elevation, temperature and water quality. Especially, it was confirmed that spatial distributions of nutrients and algae biomass have wide variation of transverse direction. Also, from the analysis of algal growth limiting factor, it was found that phosphorous loadings from Gap and Miho streams to Sejong weir induce eutrophication and algal bloom. The scenario of pollutant load reduction from Gap and Miho streams showed a significant effect on the improvement of water quality; 4.7~18.2% for Chl-a, 5.4~21.9% for TP at Cheongwon-1 site, and 4.2~ 17.3% for Chl-a and 4.7~19.4% for TP at Yeongi site. In addition, the eutrophication index value, identifying the tropic status of the river, was improved. Meanwhile, flow control of Daecheong Dam and Sejong weir showed little effect on the improvement of water quality; 1.5~2.4% for Chl-a, 2.5~ 3.8% for TP at Cheongwon-1 site, and 1.2~2.1% for Chl-a and 0.9~1.5% for TP at Yeongi site. Therefore, improvement of the water quality in Gap and Miho streams is essential and a prerequirement to meet the target water quality level of the study area.

• Journal of Environmental Impact Assessment
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• v.30 no.5
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• pp.271-296
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• 2021

Since the thermal stratification in a reservoir inhibits the vertical mixing of the upper and lower layers and causes the formation of a hypoxia layer and the enhancement of nutrients release from the sediment, changes in the stratification structure of the reservoir according to future climate change are very important in terms of water quality and aquatic ecology management. This study was aimed to develop a data-driven inflow water temperature prediction model for Daecheong Reservoir (DR), and to predict future inflow water temperature and the stratification structure of DR considering future climate scenarios of Representative Concentration Pathways (RCP). The random forest (RF)regression model (NSE 0.97, RMSE 1.86℃, MAPE 9.45%) developed to predict the inflow temperature of DR adequately reproduced the statistics and variability of the observed water temperature. Future meteorological data for each RCP scenario predicted by the regional climate model (HadGEM3-RA) was input into RF model to predict the inflow water temperature, and a three-dimensional hydrodynamic model (AEM3D) was used to predict the change in the future (2018~2037, 2038~2057, 2058~2077, 2078~2097) stratification structure of DR due to climate change. As a result, the rates of increase in air temperature and inflow water temperature was 0.14~0.48℃/10year and 0.21~0.41℃/10year,respectively. As a result of seasonal analysis, in all scenarios except spring and winter in the RCP 2.6, the increase in inflow water temperature was statistically significant, and the increase rate was higher as the carbon reduction effort was weaker. The increase rate of the surface water temperature of the reservoir was in the range of 0.04~0.38℃/10year, and the stratification period was gradually increased in all scenarios. In particular, when the RCP 8.5 scenario is applied, the number of stratification days is expected to increase by about 24 days. These results were consistent with the results of previous studies that climate change strengthens the stratification intensity of lakes and reservoirs and prolonged the stratification period, and suggested that prolonged water temperature stratification could cause changes in the aquatic ecosystem, such as spatial expansion of the low-oxygen layer, an increase in sediment nutrient release, and changed in the dominant species of algae in the water body.

• Economic and Environmental Geology
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• v.53 no.6
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• pp.729-742
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• 2020

In this study, we analyze seabottom conditions and characteristics integrated with topographic data, seafloor mosaic, underwater images and orthophoto(drone) of soft-hard bottom area around the Sib-Ri rock in the northern shore of the East Sea(Gyeongpo Beach, Gangneung). We obtained field survey data around the Sib-Ri rock(about 600 m × 600 m). The Sib-Ri rock is formed by two exposed rocks and surrounding reef. The artificial reef zone made by about 200 ~ 300 structures is shown the western area of the Sib-Ri rock. The underwater rock region is extended from the southwestern area of the exposed the Sib-Ri rock with 9 ~ 11 m depth range. The most broad rocky seabottom area is located in the southwestren area of the Sib-Ri rock with 10 ~ 13 m depth range. The study area were classified into 4 types of seabottom environment based on the analysis of bathymetric data, seafloor mosaics, composition of sediments and images(underwater and drone). The underwater rock zones(Type I) are the most distributed area around the Sib-Ri Rock(about 600 m × 600 m). The soft seabottom area made by sediments layer showed 2 types(Type II: gS(gravelly Sand), Type III: S(Sand)) in the areas between underwater rock zones and western part of the Sib-Ri rock(toward Gyeongpo Beach). The artificial reef zone with a lot of structures is located in the western part of the Sib-Ri rock. Marine algae(about 6 species), Phylum porifera(about 2 species), Phylum echinodermata(about 3 species), Phylum mollusca(about 3 species) and Phylum chordata(about 2 species) are dominant faunal group of underwater image analysis area(about 10 m × 10 m) in the northwestern part of the Sib-Ri rock. The habitat of Phylym mollusca(Lottia dorsuosa, Septifer virgatus) and Phylum arthropoda(Pollicipes mitella, Chthamalus challengeri hoek) appears in the intertidal zone of the Sib-Ri rock. And it is possible to estimate the range and distribution of the habitat based on the integrated study of orthphoto(drone) and bathymetry data. The integrated visualization and mapping techniques using seafloor mosaic images, sediments analysis, underwater images, orthophoto(drone) and topographic data can provide and contribute to figure out the seabottom conditions and characteristics in the shore of the East Sea.