• Title/Summary/Keyword: AK1

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Purification and Characterization of Very Low Density Lipoprotein in Commercial Broiler and Crossbred Village Chickens by Fast Protein Liquid Chromatography

  • Tan, B.K.;Foo, H.L.;Loh, Teck Chwen;Norhani, A.;Zulkifli, I.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.12
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    • pp.1780-1785
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    • 2005
  • Very low density lipoprotein (VLDL) of commercial broiler (CB) and crossbred village chicken (AK) was purified using Fast Protein Liquid Chromatography (FPLC). The fraction collected was then confirmed as VLDL using 4% polyacrylamide gel electrophoresis and transmission electron microscopy (TEM). The particle size of VLDL is 46.8${\pm}$8.6 nm. The VLDL fraction was then subfractionated and the apolipoprotein (apo) profile was studied by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDSPAGE). The CB and AK have almost similar types of apo in both subfractions 1 and 2. The AK showed the presence of apoAI, AIV, D and E whereas the CB had apo AIV, D, E and H. The apo AIV and apo E were present in both subfractions of AK and CB.

A Study on the Decomposition of Litter and the Leaching of Mineral Nutrients in the Stands of Pinus rigida on Mt. Gwan-ak and Pseudosasa japonica on Odong-do (관악산의 리기다소나무림과 오동도의 이대림에서 낙엽의 분해와 무기양분의 세탈에 관한 연구)

  • Chang, Nam-Kee;Heui-Baik Kim;Jun-hi Yoo
    • The Korean Journal of Ecology
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    • v.9 no.1
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    • pp.51-58
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    • 1986
  • The decay rates of organic matter were investigated and the leaching rates were determined from the concentration distribution of N,P,K, Ca and Na in the soil profile at Pinus rigida stand on Mt. Gwan-ak and at Pseudosasa japonica stand on Odong-do. In order to determine the leaching rates θo/W wazs substituted with KL in Towner's equation. Decay rates were 0.191 at Pinus stand on Mt. Gwan-ak and 0.234 at Pseudosasa stan don Odong-do. Concentration distributions of N,P, K and Na in soil profiles were corresponed with Towner's model curve. Leaching rates determined from concentration distributions were 0.086, 0.079, 0.041, 0.029, 0.096 on Mt. Gwan-ak and 0.080, 0.056, 0.051, 0.008, 0.028 on Odong-do. The soil of Pseudosasa stand showed on the whole lower leaching rates than that of Pinus stand. The soil of Pseudosasa stand showing higher decay rate and lower leaching rates contained more concentration of each nutrient than that of Pinus stand.

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Distribution of Mites on Rodents in Mt. Kwan-ak and its near Residential Area (관악산과 그 인근 주거지역에 서식하는 양치류의 진드기류 분포)

  • Lee, Byoung Un;Nam-Kee Chang;Meung-Hai Kim;Hai Poong Lee
    • The Korean Journal of Ecology
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    • v.11 no.1
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    • pp.17-28
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    • 1988
  • Five hundrad and eight rats were caught in Shiheung-dong and Mt. Kwan-ak from 1986 to 1987 in this survey. Ectoparasites were got from these rats and classified. The results were as follows: 9,191 mites (16 genera, 30 species), 408 fleas (4 genera, 4 species), and 521 lice (3 genera, 5 species) were observed in this survey. In the residential area, Rattus norvesicus(71.74%) was dominant species and in Mt. Kwan-ak Apodemus agrarius(93.62%). In these areas, the mites belonging to suborder Mesostigmata were dominant (Laelaps nuttli, 52.61%; Ornithonyssus bacoti, 36.05%). In Mt. Kwan-ak, family Frombiculidae belonging to suborder Prostigmata were dominant (Leptotromibidium orientalis, 31.04%; Leptotrombidium palpalis, 30.00%). In this way, it was discovered that the difference between the two regions in dominant species was noticeable. As for the hosts parasitized by mites, L. nuttalli in R. norvegicus (54.85%), L. nuttalli in R. rattus (32.90%), and L. orientalis in A. agrarius (31.27%) were dominant species, respectively. In the mites parasitizing on A. agrarius in each altitude, the species were more varied as the region was lower and the dominant species were more concentrated as it was higher.

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The effect of Ssukdaemeori on depression: A heartrending song of sorrow, Han Ak (Korean music, 韓樂) is wetting on YouTube sung by Bang-ul, Im (https://youtu.be/IFzFeJcVLp4)

  • Ko, Kyung-Ja;Kim, Ji-Youn
    • CELLMED
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    • v.6 no.4
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    • pp.25.1-25.2
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    • 2016
  • The purpose of this study is to examine Ssukdaemeori of Chunhyangga may have effect on depression for music therapy. Ssukdaemeori of Chunhyangga, one of the five surviving stories of pansori (a long epic song), is a touching, sad song in Han Ak (Korean music, 韓樂). In this paper, the author reports on the very sad song, Ssukdaemeori. While listening to the song, Koreans feel the same amount of despair when Ssudaemeori's heroine, Chunhyang, cries out in hopelessness and grief. The author argues that Koreans get to see the saddest parts of themselves reflected back at them in the sorrowful sight of the heroine. It is also believed that Ssukdaemeori has contributed to the healing of Koreans' indignation and pain. For example, depression is currently a social problem. However, listening to sorrowful music like Ssukdaemeori can have a good effect on people feeling sad and depressed.

Transcriptomic Insights into Abies koreana Drought Tolerance Conferred by Aureobasidium pullulans AK10

  • Jungwook Park;Mohamed Mannaa;Gil Han;Hyejung Jung;Hyo Seong Jeon;Jin-Cheol Kim;Ae Ran Park;Young-Su Seo
    • The Plant Pathology Journal
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    • v.40 no.1
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    • pp.30-39
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    • 2024
  • The conservation of the endangered Korean fir, Abies koreana, is of critical ecological importance. In our previous study, a yeast-like fungus identified as Aureobasidium pullulans AK10, was isolated and shown to enhance drought tolerance in A. koreana seedlings. In this study, the effectiveness of Au. pullulans AK10 treatment in enhancing drought tolerance in A. koreana was confirmed. Furthermore, using transcriptome analysis, we compared A. koreana seedlings treated with Au. pullulans AK10 to untreated controls under drought conditions to elucidate the molecular responses involved in increased drought tolerance. Our findings revealed a predominance of downregulated genes in the treated seedlings, suggesting a strategic reallocation of resources to enhance stress defense. Further exploration of enriched Kyoto Encyclopedia of Genes and Genomes pathways and protein-protein interaction networks revealed significant alterations in functional systems known to fortify drought tolerance, including the terpenoid backbone biosynthesis, calcium signaling pathway, pyruvate metabolism, brassinosteroid biosynthesis, and, crucially, flavonoid biosynthesis, renowned for enhancing plant drought resistance. These findings deepen our comprehension of how AK10 biostimulation enhances the resilience of A. koreana to drought stress, marking a substantial advancement in the effort to conserve this endangered tree species through environmentally sustainable treatment.

A Study on dress and its Ornaments for farm-music (농악복식(農樂服飾)에 관한 연구(硏究))

  • Suh, Ok-Kyu
    • Journal of the Korean Society of Costume
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    • v.12
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    • pp.9-23
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    • 1988
  • This study is on the costumes for Korean traditional play, Nong-ak(farm music), and intends to analyze their aesthetic features, laying emphasis on Pilbong Nong-ak, Im sil, Chollanam-do. About its origin there are many kinds of theories; for example, the theory of hoping stability, the theory of it being related with Buddhism, the theory of martial music, etc. Shamanic, Buddhist, and martical fators that support these theories are expressed through flags, bells, drums, Deograe (half-coats), Cheonrips (sang-mo, felf hats), go-kkals(peaked hats), colored lines called 'ga-sa', which are used in Nong-ak. The characteristic of the costumes used in Pilbong Nong-ak is that it keeps its conservativeness and the costumes of its members are various and splendid. For example, leader groups' black half coats, Changbu's and Hwa-dong's red and bule over coats are remarkable. Particpants wear gok-kal or cheonrip, trousers and half coats which are the basic costumes of Korean Hanbok, and wear blak half coats or blue vests and put blue, red, and yellow lines around them. The colors and knotting methods of those lines in this region are the same with those of chollawoo-do and Kyongi province, but different form those of Kongwon and Kyong-sang province using green, red, and yellow colors. This comparison of colors shows each region's preference of peculiar colors and those colors coincide with colors used in flags. The research on the aesthetic characteristics of Nong-ak clothes through each region's clothes tells us that these can be linear clothes which have expressiveness as stage clothes used in Madangori, the play which is performed in the field, and modern spatial formativeness. Those characteristics are as follows; 1. The expressions of a rhythmical and daring round line by turning a long line of sang-mo. 2. Various rhythms according to the attaching methods. 3. The expressions of thick, simple, and daring color lines. 4. Natural beauty of materials. 5. The popular simplicity and non-technicality 6. The beauty of five-direction colors, Oriental ideal colors Consequently in this study our national consciousness of beauty are examined through clothes. It is suggested that the aesthetic characteristics of Nong-ak clothes and ornaments should be effectively expressed, for this purpose. interests in participants' clothes should be increased in order to prevent the confusion of each region's features. Also it is necessary to improve color lines, their length, width, and knotting methods, and beautify instrument. Finally this study intends to bring the reappraisal about the art of Nong-ak clothes and its re-establishment in view of modern aesthetic consciousness.

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Development of effective heparin extraction method from pig by-products and analysis of their bioavailability

  • Lee, Da Young;Lee, Seung Yun;Kang, Hea Jin;Park, Yeonhwa;Hur, Sun Jin
    • Journal of Animal Science and Technology
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    • v.62 no.6
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    • pp.933-947
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    • 2020
  • This study was conducted to develop an effective heparin extraction method by using low-cost and highly effective enzymes from six pig by-products (liver, lung, heart, stomach, small intestine, and large intestine), and analyze their bioavailability. Low-cost and highly effective enzymes (alkaline-AK and papain) and a common enzyme (trypsin) were used for the heparin extraction. The angiotensin I- converting enzyme (ACE) inhibitory activity and the antimicrobial activity of extracted heparin were analyzed to verify their bioavailability. The average amount of heparin extracted per kilogram of pig by-products was 439 mg from the liver, 127 mg from the lung, 398 mg from the heart, 261 mg from the stomach, 197 mg from the small intestine, and 239 mg from the large intestine. Various enzymes were used to extract heparin, and the amount of extracted heparin was similar. Based on 1 g of pig by-product, the enzymes trypsin, papain, and alkaline-AK could extract 1,718 mg, 1,697 mg, and 1,905 mg of heparin, respectively. Heparin extracted from pig by-products showed antihypertensive activity and antimicrobial activity against Staphylococcus aureus at low populations. These results indicated that heparin can be obtained from pig by-products at a low cost.

Identification of Agrobacterium tumefaciens from Soil and Transformation of Maize (토양으로부터의 Agrobacterium tumefaciens의 분리, 동정 및 옥수수의 형질전환에 이용)

  • 노광수;강봉중
    • KSBB Journal
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    • v.7 no.3
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    • pp.191-200
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    • 1992
  • Several strains of Agrobacterium tumefaciens were isolated from soil in the Taegu area and characterized to develop some useful vector systems for higher plant genetic engineering. The selected colonies had a unique form, and strains from the colonies were capable of tumor formation on the sunflower leaf surface. They had a large plasmid. The restriction analysis showed that they were another kinds of Ti plasmic compared with C58 and Ach5. The isolated strains were identified as the nopaline type and also as biovar 1 A. tumefaciens, according to their tumor morphology, blophyslcal and biochemical characteristics. One of the isolated strains, AK204 was transformed with binary vector (pGA642), having selectable marker (Kmr, Tcr). Furthermore, maize tissue cells were transformed by cocultivation with AK204/pGA642, and the transformants were selected on the selective medium and identified using PAGE patterns of their soluble proteins.

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Differences in Electric Potential of Meridian System - Comparing Electric Potentials of Patients with Arthroncus of Knee - (슬안풍 환자의 십이경맥 전위측정 연구)

  • Nam, Bong-Hyun;Choi, Hwan-Soo
    • Korean Journal of Acupuncture
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    • v.21 no.4
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    • pp.21-30
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    • 2004
  • Objectives : Assuming that the characteristic of meridian system has been similar to this of electric potentials in human body and that measurements of electric potential at well(井穴) and sea(合穴) points in branches of the twelve meridians(WSBTM) will be representative of measurements of the twelve meridians, to measure the electric potentials of 13 patients with Arthroncus of Knee(슬안풍<膝眼風>, AK), to find out the characteristic of meridian system in patients with AK. Methods : Electric potentials of well and sea points in the meridians in twenty one patients with the pain in the lion diagnosed as AK were repeatedly measured by physiograph(PowerLab). Measurements of those electric potentials were analyzed by factor analysis. Results : Their electric potentials at the left and right side were factors which are different from each side. In the left side, Factor 1 included Small Intestine, Pericardium, Spleen, Kidney meridian, and Factor 2 included Heart, Tripple Energizer, Bladder, Liver meridian. Factor 3 included Large Intestine, Stomach, Gall bladder meridian, and Factor 4 included Lung meridian. In the right side, Factor 1 included Heart, Pericardium, Tripple Energizer, Spleen, Bladder meridian and Factor 2 included Lung, Liver, Gall bladder meridian. Factor 3 included Small Intestine, Stomach, Kidney meridian and Factor 4 included Large Intestine Meridian. Conclusions : The electric potentials of AK differ from those of normal bodies as well as of bodies with other diseases-shoulder lesions, waist lesions, Lumbago due to Strain and Contusion-. Thus electric potentials of well and sea points might be the representative meridian to show their characteristics.

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The Action of Extract of Aconitum koreanum R. Raymond on Isolated Clam Heart (우리나라 백부자의 적출 조개 심장운동에 대한 작용)

  • Ha, Byoung-Kuk;Kim, Yoo-Sung;Kim, Won-Ja;Park, Chul-Hoon
    • The Korean Journal of Pharmacology
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    • v.8 no.1
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    • pp.15-25
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    • 1972
  • Korean aconitum (Aconitum koreanum R. Raymond) as one of the botanical crude drugs which pertain to helleboraceae has been extensively applied in Chinese medicine during the past decades. It has been particularly used in immortal tonic among the folk remedies in China, however, its general uses comprehend diuresis, cardiotonic, analgesia, neuralgia, gout and, furthermore, even neoplastic effect. The components of aconitum have been acknowledged as aconitine, mesaconitine, hypaconitine, aconine and so on. The main ingredient, aconitine has the advantage of causing the atrial fibrillation, but, its pharmacological research has not been fully elucidated. Although there are many reports with regard to the pharmacological effects on the motility of several animal hearts, their conclusions have not been regretfully coincided yet. The authors hereby paid attention to this point of view and made experiment to examine the relationship between the alcohol extract of Korean aconitum and the motility of the isolated clam heart, making the use of several drugs related to the heart such as serotonin, acetylcholine, pilocarpine, physostigmine, barium chloride, procaine and quinidine. The cardiac movement of the isolated clam (Meretrix lusoria) heart in the standard sea water solution was recorded with the electric kymograph according to the Magnus method. The results of the experiment are as follows. 1. The motility of the isolated clam heart represents the tendency of gradual inhibition in proportion to the concentration of AK-A $10^{-4}$, $5{\times}10^{-4}$, and $10^{-3}$. 2. The cardiac inhibitory effect of AK-A $10^{-3}$ antagonizes the motility of the isolated clam heart pretreated with serotonin $10^{-6}$. 3. The cardiac inhibitory effect of AK-A $10^{-3}$ antagonizes the systolic state appealed by barium chloride $10^{-3}$. 4. The systolic state caused by quinidine $10^{-4}$ is not inhibited by AK-A $10^{-3}$.

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