• 생명과학회지
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• 제22권11호
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• pp.1587-1594
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• 2012

3C는 진핵세포의 핵에서 크로마틴의 입체 구조/구성을 알아보는 연구 기법이다. 이 기법은 살아있는 세포를 포름알데히드로 처리하여 단백질들 사이의 결합 및 단백질과 DNA 사이의 결합을 고정시킨 후, 제한효소로 DNA를 절단하고, 그 절편들의 연결 빈도를 측정함으로써 DNA 절편 사이의 물리적 근접성을 보여준다. 이 기법을 이용하여 복합 유전자 좌위인 ${\beta}$-글로빈 좌위에서 locus control region이 전사가 활발한 유전자와 가까이 위치하고 있음이 밝혀졌으며, 이러한 결과는 크로마틴 입체 구조가 유전자 전사 조절에 관여함을 나타낸다. 또한 3C 기법은 ChIP 및 genome-wide sequencing과 결합되어 다양한 기술로 진화되었다. 본 총설은 3C의 원리 및 과정을 짚어보고, 3C 기법으로 밝혀진 ${\beta}$-글로빈 좌위의 크로마틴 입체 구조를 설명하고자 하며, 나아가 3C를 기본으로 한 다양한 응용 연구 기법도 살펴보고자 한다.

• 한국운동역학회지
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• 제14권1호
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• pp.145-160
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• 2004

The purposes of this study to provide quantitative data in necessary to advance techniques kinematic analysis of Cucarachas which is an action of Rumba. Then, this study is performed on 5 female players who have won within the third prize at a national athletic meeting. When whole foot reached to floor, Displacement of right-left hip joint (until $E1{\sim}E3$ average moved 15.15cm)is found at right-left direction since the hip joint is turned to right back. On the other side, large displacement is shown because Rumba Cucaracha Movement is expressed by maximum shift of hip joint to right and left direction. Displacement of right hip joint(E3$57.40{\pm}7.46$) is found in front and in rear direction since hip joint is moved in rear and in front to turn the hip joint. It may be stated that this is ideal displacement expressed by movement of whole body with artistic poise and presentation because role of hip joint is very important in technical and artistic side. Angle of right shoulder joint E2($105.44{\pm}9.64$) is got wider. It may be stated that player shifts up and abduct elbow joint to right since center of gravity of player is exceedingly shifted to right in this motion of Cucarachas. On the other hand, since this motion is abducted right elbow and shrunk external abdominal oblique to him center of body to left front of hip joint, the angle becomes narrow. It is shown that angle of knee in right knee joint E4($75.44{\pm}2.61$) is large since right leg and hip joint is turned by foot using reaction of ground and so center of body is shifted to left. Large angle of ankle E4($134.40{\pm}10.50$) in Cucaracha Movement is shown by the action of twist force using narrow part of foot and compression force against ground with adduction speed of arm. The various kinematic analyses associated with motions of dance sport have not been sufficiently peformed so far, and thus a number of research projects for dance sport should be proposed and performed to be continuous.

• 한국축산식품학회지
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• 제32권2호
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• pp.241-246
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• 2012

This report outlines the development of a rapid, simple, and sensitive detection system for pathogenic bacteria using a capillary electrophoresis-based, single strand conformation polymorphism (CE-SSCP) combined with PCR. We demonstrate that this method, used with primers targeting the V4 region of the16S rRNA gene, is capable of the simultaneous detection of 10 microbes that could be associated with foodborne illness, caused by animal-derived foods: Salmonella enterica, Listeria monocytogenes, Escherichia coli O157:H7, Campylobacter jejuni, Staphylococcus aureus, Bacillus cereus, Clostridium perfringens, Yersinia enterocolitica, Vibrio parahaemolyticus, and Enterobacter sakazakii. The traditional detection techniques are time-consuming and labor-intensive, due to the necessary task of separate cultivation of each target species. As such, the CE-SSCP-PCR method, that we have developed, has the potential to diagnose pathogens rapidly, unlike the traditional technique, in order to prevent foodborne illness in a much more efficient manner.

• 한국기록관리학회지
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• 제20권1호
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• pp.89-114
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• 2020

본 연구는 국·공립대학교 기록관리 담당자의 직무를 명확히 밝히고 IPA 기법을 활용하여 직종별, 경력별로 업무에 대해 인지하는 차이와 특성을 밝혀 업무의 역량강화를 위한 기초자료를 제시하는 것이 목적이다. 이를 위해 국·공립대학교 기록관리 담당자의 업무를 분석하고 설문조사를 실시하였으며, 통계분석과 IPA Matrix 분석을 실시하였다. 분석의 결과를 토대로 도출한 문제점에 대한 개선방안으로 국·공립대학교 기록연구사의 배치 의무화, 업무 수행을 위한 예산 확보, 다양한 경로를 통한 대학역사기록물 수집, 기록관리기관 평가 보완 및 수정, 경력별에 따른 직무 역량 조절 등을 제안하였다.

• 한국지구과학회지
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• 제30권4호
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• pp.513-526
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• 2009

해양 염분의 단위가 퍼밀(%o)에서 다른 단위로 바뀐 지 오랜 세월이 지났다. 하지만 우리나라 중학교, 고등학교 과학 및 지구과학 교과서는 여전히 오래 전에 정의한 염분 단위인 퍼밀(%o)을 사용하고 있다. 본 연구의 목적은 염분단위의 변화와 측정 기술에 대한 역사적 발달 과정을 개괄적으로 다루고, 염분 단위들 사이의 차이를 제시하며, 그리고 교과서에서의 염분 단위 수정에 대한 필요성과 타당성을 강조하는데 있다. 이를 위해 제 7차 교육과정에 기초한 27종의 중등 과학교과서에서 다루고 있는 염분 단위의 표현, 염분의 정의들을 조사하였다. 분석 결과는 1967년부터 2008년까지 한국해양학회지에 게재된 논문들에서 사용하고 있는 염분 단위 사용의 빈도수와 비교하였다. %o 사용의 백분율은 교과서 본문에서 96.3%, 그림과 표에서 83.8%에 달하였다. 이에 반해 과학적 논문들은 1994년부터 psu를 사용하기 시작하였으며, 2004년 이후로는 %o을 거의 사용하지 않고 있었다.

• 분석과학
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• 제7권4호
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• pp.493-504
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• 1994

Nb steel에 있어서의 석출물의 형상과 구조를 SEM, TEM과 XRD를 이용하여 연구하였고 추출물들의 정량분석을 ICP-AES를 사용하여 실시하였다. 석출물 추출 방법으로는 정전위 전해추출법을 사용하였고 전해액으로는 10% AA-methanol 용액과 15% Na-citrate 용액을 사용하여 비교하였다. 전해추출 전위는 10% AA-methanol 용액에서는 -100mV, 15% Na-citrate 용액에서는 -250mV로 선택하였고 이 전위에서 Nb 석출물을 분리하는 데 적당하였다. 추출 잔사 중의 $Fe_3C$의 크기는 $0.2{\sim}1.0{\mu}m$ 정도였고 Nb 탄질화물의 크기는 $0.003{\sim}0.1{\mu}m$ 정도였다. 0.1% C-0.007% Nb-0.004% N의 조성을 가진 강에서 Nb 석출물의 조성은 $NbC_{0.65}N_{0.2}$인 것으로 나타나 입방정계의 탄질화물인 것으로 판명되었다.

• The Korean Journal of Physiology and Pharmacology
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• 제24권6호
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• pp.441-452
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• 2020

In vivo animal models are limited in their ability to mimic the extremely complex systems of the human body, and there is increasing disquiet about the ethics of animal research. Many authorities in different geographical areas are considering implementing a ban on animal testing, including testing for cosmetics and pharmaceuticals. Therefore, there is a need for research into systems that can replicate the responses of laboratory animals and simulate environments similar to the human body in a laboratory. An in vitro two-dimensional cell culture model is widely used, because such a system is relatively inexpensive, easy to implement, and can gather considerable amounts of reference data. However, these models lack a real physiological extracellular environment. Recent advances in stem cell biology, tissue engineering, and microfabrication techniques have facilitated the development of various 3D cell culture models. These include multicellular spheroids, organoids, and organs-on-chips, each of which has its own advantages and limitations. Organoids are organ-specific cell clusters created by aggregating cells derived from pluripotent, adult, and cancer stem cells. Patient-derived organoids can be used as models of human disease in a culture dish. Biomimetic organ chips are models that replicate the physiological and mechanical functions of human organs. Many organoids and organ-on-a-chips have been developed for drug screening and testing, so competition for patents between countries is also intensifying. We analyzed the scientific and technological trends underlying these cutting-edge models, which are developed for use as non-animal models for testing safety and efficacy at the nonclinical stages of drug development.

• Macromolecular Research
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• 제16권3호
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• pp.204-211
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• 2008

A platinum-catalyzed polyelectrolyte porous membrane was prepared by solid-state compression of electrospun polystyrene (PS) fibers and in-situ metallization of counter-balanced ionic metal sources on the polymer surface. Using this ion-exchange metal-polymer composite system, fiber entangled pores were formed in the interstitial space of the fibers, which were surrounded by sulfonic acid sites ($SO_3^-$) to give a cation-selective polyelectrolyte porous bed with an ion exchange capacity ($I_{EC}$) of 3.0 meq/g and an ionic conductivity of 0.09 S/cm. The Pt loading was estimated to be 16.32 wt% from the $SO_3^-$ ions on the surface of the sulfonated PS fibers, which interact with the cationic platinum complex, $Pt(NH_3)_4^{2+}$, at a ratio of 3:1 based on steric hindrance and the arrangement of interacting ions. This is in good agreement with the Pt loading of 15.82 wt% measured by inductively coupled plasma-optical emission spectroscopy (ICP-OES). The Pt-loaded sulfonated PS media showed an ionic conductivity of 0.32 S/cm. The in-situ metallized platinum provided a nano-sized and strongly-bound catalyst in robust porous media, which highlights its potential use in various electrochemical and catalytic systems.

• Journal of Radiation Protection and Research
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• 제46권3호
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• pp.98-105
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• 2021

Background: In recent events of the coronavirus disease 2019 (COVID-19) pandemic, computed tomography (CT) scans are being globally used as a complement to the reverse-transcription polymerase chain reaction (RT-PCR) tests. It will be important to be aware of major organ dose levels, which are more relevant quantity to derive potential long-term adverse effect, for Korean pediatric and adult patients undergoing CT for COVID-19. Materials and Methods: We calculated organ dose conversion coefficients for Korean pediatric and adult CT patients directly from Korean pediatric and adult computational phantoms combined with Monte Carlo radiation transport techniques. We then estimated major organ doses delivered to the Korean child and adult patients undergoing CT for COVID-19 combining the dose conversion coefficients and the international survey data. We also compared our Korean dose conversion coefficients with those from Caucasian reference pediatric and adult phantoms. Results and Discussion: Based on the dose conversion coefficients we established in this study and the international survey data of COVID-19-related CT scans, we found that Korean 7-year-old child and adult males may receive about 4-32 mGy and 3-21 mGy of lung dose, respectively. We learned that the lung dose conversion coefficient for the Korean child phantom was up to 1.5-fold greater than that for the Korean adult phantom. We also found no substantial difference in dose conversion coefficients between Korean and Caucasian phantoms. Conclusion: We estimated radiation dose delivered to the Korean child and adult phantoms undergoing COVID-19-related CT examinations. The dose conversion coefficients derived for different CT scan types can be also used universally for other dosimetry studies concerning Korean CT scans. We also confirmed that the Caucasian-based CT organ dose calculation tools may be used for the Korean population with reasonable accuracy.

• 산업식품공학
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• 제13권4호
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• pp.335-340
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• 2009

본 연구는 초미세분쇄기술을 이용하여 옥수수전분의 입자구조파괴가 이루어졌을 때 분자구조적, 물리적 변화가 어떻게 이루어지는지를 구명하고자 하였다. 초미세 분쇄처리 후 옥수수전분의 평균직경은 약 50% 감소가 이루어졌으며 비표면적은 567% 증가하였다. 초미세분쇄처리 전 후의 옥수수전분에 대한 분자량분포를 GPC로 측정한 결과 분쇄 후 저분자량의 Peak II의 면적이 21.0%에서 86.5%로 상승하였다. 손상전분 함량은 각각 9.63%와 83.57%로 초미세분쇄처리에 의하여 크게 증가하였다. 옥수수전분의 경우는 겔(gel)을 형성하였으나 초미세분쇄처리 후에는 전분의 분쇄과정에서 전분입자파괴와 아울러 옥수수전분의 분자량이 저분자화 되면서 겔 형성능력이 크게 저하되었다.