• Journal of environmental and Sanitary engineering
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• v.12 no.2
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• pp.37-42
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• 1997

The toxic and detoxifying effects of cadmium and zinc on rat plasma ACTH and serum cortisol levels were investigated in rats. Rats were injected by i.p. with saline (0.9%), cadmium chloride (0.25 or 0.5mg/kg body weight) and pretreated with zinc chloride (4mg/kg body weight) before cadmium chloride treatment 1 or 2 weeks, respectively. The ACTH levels were no significant differences in cadmium 0.25mg/kg-treated group, but were significantly decreased in cadmium 0.5mg/kg-treated group compared with normal group. The ACTH levels after zinc pretreatment for 1 week were significantly increased but zinc pretreatment for 2 week were no difference. The serum cortisol levels of cadmium treated rats were significantly decreased, but were increased in zinc pretreated rats. The results showed that the zinc have some protective effect on cadmium toxicity in rats.

• BMB Reports
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• v.46 no.2
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• pp.80-85
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• 2013

We investigated the temporal alterations of adrenocorticotropic hormone (ACTH) immunoreactivity in the hippocampus after seizure onset. Expression of ACTH was observed within inter-neurons in the pre-seizure group of seizure sensitive gerbils, whereas its immunoreactivities were rarely detected in seizure resistant gerbil. Three hr after the seizure, ACTH immunoreac-tivity was significantly increased in interneurons within all hippocampal regions. On the basis of their localization and morphology through immunofluorescence staining, these cells were identified as $GABA_A$ ${\alpha}1$-containing interneurons. At the 12 hr postictal period, ACTH expression in these regions was down-regulated, in a similar manner to the pre-seizure group of gerbils. These findings support the increase in ACTH synthesis that contributes to a reduction of corticotrophin-releasing factor via the negative feedback system which in turn provides an opportunity to enhance the excitability of GABAergic interneurons. Therefore, ACTH may play an important role in the reduction of excitotoxicity in all hippocampal regions.

• The Korea Journal of Herbology
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• v.24 no.1
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• pp.141-150
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• 2009

Objectives : The investigation of the antidepressant effects of Citri Reticulatae Viride Pericarpium (CR) Methods : we performed the forced swimming test. Also the expression of Tyrosine Hydroxylase (TH) was measured with immunohistochemical method at the Ventral Tegmental area (VTA), Locus coeruleus (LC). The Adrenocorticotropic Hormone (ACTH) level was measured in plasma. Results: 1. The duration of immobility in the forced swimming test was significantly decreased in the CR 100 mg/kg, CR 400 mg/kg groups, comparing with the control group (p < 0.05, p < 0.001). 2. TH expressions in the VTA, LC were significantly reduced in the CR 100mg/kg and CR 400mg/kg treated group, comparing with the control group (p < 0.05, p < 0.001). 3. ACTH expression in plama was significantly reduced in the CR 100 mg/kg treated group, comparing with the control group (p < 0.05). Conclusions : According to the above results, it can be considered that Citri Reticulatae Viride Pericarpium has antidepressant effect through the reduction of TH expression at VTA, LC and ACTH level in plasma.

• The Journal of Korean Physical Therapy
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• v.6 no.1
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• pp.5-15
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• 1994

The purpose of this study was to examine the effect of electroanalgeia and $\beta-endorphin$ action by acupuncture-like (Lof/Hil) transcutaneous electrical nerve stimulation (TENS) applied to acupuncture points. Twelve healthy adult male aged between 19 ann 25 were randomly assigned to TENS group (n=6) and naloxone group (n=6). Subjects of both groups were strongly stimulated TENS with 4 pps and $200{\mu}s$ for 30 minutes on the LI 3 and LI 10 meridian points of dominant am. Naloxone group was injected naloxone hydrochloride before TENS application. The experimental pain threshold was measured by chronaxie meter CX-2 on the distal end of radius just before and after TENS application. The levels of plasma $\beta-endorphin$ and ACTH. serum cortisol and urinary 17-OHCS were analyzed by radioimmunoassay (RIA) kits before and after TENS application. In TENS group, there was a significant increase of experimental pain threshold (p<0.01), plasma $\beta-endorphin$ level (p<0.05), serum cortisol level (p<0,001) and urinary 17-OHCS levels (p<0.05) after TENS application. The plasma ACTH level was not significantly increased, but it showed an increasing tendency. In naloxone group, although there was a decreasing trend, ACTH and cortisol level did not show a significant change, but $\beta-endorphin$ and 17-OHCS level were significantly decreased (p<0.01). The result of this study stewed that acupuncture-like TENS induced analgesic effect, such that the levels of plasma $\beta-endorphin$, plasma ACTH, serum cortisol and urinary 17-OHCS were concomitantly increased with experimental pain threshold. It is suggested that the analgesic mechanism of the acupuncture-like TENS probably related to endogenous opioid component such as $\beta-endorphin$.

• Journal of Periodontal and Implant Science
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• v.5 no.1
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• pp.18.1-18.1
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• 1975

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.6
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• pp.806-812
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• 2003

Attempts have been made to establish relationship between the response to ACTH challenge in female calves, growth and first lactation performance. A total of 19 Holstein calves weighing 100 kg i. v. were given 0.50 IU of ACTH/kg $BW^{.75}$ (EXACTHIN inj., Richter G., Budapest) at 60 days of age. Serial blood samples were taken at times 0, 0.5, 1, 2, 3, 4 and 5 hours and analyzed for cortisol, glucose insulin and FFA levels. From challenge series the area under the curve from time of administration and the following 5 h were calculated. Negative, and mostly loose relationship between response to ACTH challenge for cortisol, insulin, or FFA and ADWG during growth have been established (p>0.05) with positive one for glucose. Bivariate coefficients of correlation varied within the range from -0.35 to 0.15. Estimations reveal negative correlation between the length of first lactation and cortisol or insulin (r=-0.80, p<0.001 and r=-0.45, p<0.10, resp.) Close association between cortisol or insulin and actual first lactation milk yield was found (r=-0.48, p<0.10; r=-0.64, p<0.01, resp.). Close relationship between the response to ACTH challenge and milk protein yield was present only for insulin (r=-0.59, p<0.05).

• 대한핵의학회:학술대회논문집
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• 1977.05a
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• pp.96.2-96.2
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• 1977

• Korean Journal of Veterinary Research
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• v.39 no.1
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• pp.77-84
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• 1999

Anesthetic agents are useful in inducing the anesthesia for surgical operations and various biological experiments, but they can disturb the body homeostasis and cause the stress in animals. Much efforts have been directed on reducing such side effects of anesthesia. In this work, we measured the serum ACTH, corticosterone and glucose concentration in rabbits to compare the degree of stress induced by two commonly-used anesthetics, ketamine, xylazine, and the combination of xylazine and ketamine. 1. The anesthesia was induced in about 10 min in the rabbits treated with xyalzine, ketamine and xylazine-ketamine. The duration of complete loss of righting reflex were 12, 13 and 115 min in the groups treated with xylazine, ketamine and xylazine-ketamine, respectively. 2. Serum ACTH concentrations in all treatment groups were higher than those in control group. At 30 min after the administration of the drugs, serum ACTH levels in ketamine-treated group were significantly higher than those in control, xylazine- and xylazine-ketamine-treated groups. However, at 1, 2, 5 and 9 hours after the drug administration, serum ACTH levels in xylazine-treated-group were higher than those in control. 3. Serum corticosterone levels in xylazine- and xylazine-ketamine-treated groups were lower than those in control or ketamine-treated groups at 0.5 and 1 hour after the administration. However, at 5 and 9 hours after the administration, serum corticosterone levels in xylazine- and xylazine-ketamine-treated groups were significantly higher than those in ketamine-treated group or control. 4. Serum glucose levels transiently increased to 3 times of the pre-injection levels at 0.5 and 1 hours after the administration in xylazine or xylazine-ketamine-treated groin, but were not changed in control and ketamine-treated group. These results indicate that xylazine-induced stress lasts longer than ketamine-induced, suggesting that the difference in stress-related hormone levels during anesthesia could be due to the differences in modes of actions of individual drugs used and the depth of anesthesia.

• Korean Journal of Veterinary Research
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• v.51 no.4
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• pp.315-318
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• 2011

A dog (5.6 kg, 4-year-old, castrated male, Miniature pinscher) with diffuse symmetric alopecia on the chest and excessive scales was referred to Veterinary Medical Teaching Hospital of Chungnam National University, Korea. On physical examination, diffuse alopecia on ventral aspect of the neck and caudomedial thighs, and severe scales were presented. Dermatologic tests of lesions revealed bacterial infections. Histopathologically, atrophy of hair bulb was observed in the severely alopecic lesion of caudomedial thighs. ACTH stimulation test was performed to differentiate the endocrine disorder related to generalized alopecia. The pre-ACTH serum testosterone and progesterone concentrations were above normal ranges and the post-ACTH serum testosterone concentration was high. The pre- and post-ACTH serum estradiol and cortisol concentrations were within normal ranges. Canine pattern alopecia was diagnosed based on history, physical examination, hormonal assay and dermatohistopathologic examination. After 3 months of melatonin administration, multifocal alopecia on the trunk was improved and general hair regrowth was identified.

• Korean Journal of Biological Psychiatry
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• v.4 no.2
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• pp.272-278
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• 1997

Many investigators are trying to elucidate the pathogenesis of psychiatric disorders on the basis of neuroendocrine responses to stimulation or perturbation. Dexamethasone(DEX) suppression has been the most widely utilized as the prototypical challenge test. Dexamethasone suppression test(DST) has proven to be valuable in diagnosing the depressive spectrum disorder. Reported specificity of diagnosis of depression is relatively high, but sensitivity is limited. Some researchers used the combination of dexamethasone and corticotropin releasing hormone(CRH) in order to improve the sensitivity. They reported that combined DEX/CRH test is more sensitive than DST alone. In this study the authors modified the DEX/CRH test, i.e., we administered the insulin instead of CRH. Total subjects were 28(7 normal controls, 10 manic patients, 11 schizophrenic patients). Subjects were taken DEX(1.5mg p.o.) at 11 p.m., insulin 16 hours later(0.1 unit/kg i.v.). Five blood samples for the determination of cortisol and ACTH were serially drawn at 15 minute interval. The results are as followings : 1) The cortisol and ACTH levels of manic subjects increased following insulin administration. Manic subjects showed higher levels of cortisol and ACTH than schizophrenic and normal control subjects. The cortisol and ACTH levels of schizophrenic and normal control subjects did not show gross changes. 2) The sensitivity of the test was lower than that of reported DEX/CRH test.