• Journal of Microbiology and Biotechnology
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• v.30 no.7
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• pp.982-995
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• 2020

A putative multidrug efflux gene, yddA, was cloned from the Escherichia coli K-12 strain. A drug-sensitive strain of E. coli missing the main multidrug efflux pump AcrB was constructed as a host and the yddA gene was knocked out in wild-type (WT) and drug-sensitive E. coliΔacrB to study the yddA function. Sensitivity to different substrates of WT E.coli, E. coliΔyddA, E. coliΔacrB and E. coliΔacrBΔyddA strains was compared with minimal inhibitory concentration (MIC) assays and fluorescence tests. MIC assay and fluorescence test results showed that YddA protein was a multidrug efflux pump that exported multiple substrates. Three inhibitors, ortho-vanadate, carbonyl cyanide m-chlorophenylhydrazone (CCCP), and reserpine, were used in fluorescence tests. Ortho-vanadate and reserpine significantly inhibited the efflux and increased accumulation of ethidium bromide and norfloxacin, while CCCP had no significant effect on YddA-regulated efflux. The results indicated that YddA relies on energy released from ATP hydrolysis to transfer the substrates and YddA is an ABC-type multidrug exporter. Functional study of unknown ATP-binding cassette (ABC) superfamily transporters in the model organism E. coli is conducive to discovering new multidrug resistance-reversal targets and providing references for studying other ABC proteins of unknown function.

• The Korean Journal of Physiology and Pharmacology
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• v.13 no.1
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• pp.33-37
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• 2009

Anti-inflammatory factor(AIF) is a water soluble extract of three herbs, Panax notoginseng(Burk.) F. H. Chen, Rehmannia glutinosa Libosch and Eleutherococcus senticosus. The present study aimed to investigate the safety and efficacy of herb extracts, AIF, on Korean knee osteoarthritis patients for six weeks. Fifty seven patients with knee osteoarthritis, ranging from 43 to 73 years of age, who fulfilled the "American College of Rheumatology"(ACR) classification of idiopathic osteoarthritis of knee and radiographic criteria were randomly selected and enrolled for the study. After initial screening and resting period, two capsules each of AIF(Each capsule contains; 400 mg) and similar identical placebo were administered twice a day to both groups. Pain intensity at second, fourth, and sixth weeks of study as well as one week after discontinuation of drugs was assessed by using 100 mm visual analogue scale(VAS). Changes in the Korean version of the Western Ontario and McMaster Universities(K-WOMAC) index score were compared at the initiation and completion of the study. VAS assessed by patients were significantly reduced(at visit 2; $54.64{\pm}14.72$, at visit 4, $37.32{\pm}16.58$, p<0.001) after AIF administration. Results showed an improvement in the physical function of K-WOMAC scale which was significantly higher(p=0.013) in AIF than placebo group, and decreases of total K-WOMAC score were also significantly higher(p=0.030) in AIF groups than placebo group. No serious adverse effect was observed, and there was no difference in incidence of adverse effect between AIF and placebo groups. In this population of Korean patients with knee osteoarthritis, AIF was found to be safe, tolerable and effective for symptomatic improvement of pain and physical function.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.4
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• pp.1693-1697
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• 2014

Background: The Bahcesehir Breast Cancer Screening Project is the first organized population based breast cancer mammographic screening project in Turkey. The objective of this prospective observational study was to demonstrate the feasibility of a screening program in a developing country and to determine the appropriate age (40 or 50 years old) to start with screening in Turkish women. Materials and Methods: Between January 2009 to December 2010, a total of 3,758 women aged 40-69 years were recruited in this prospective study. Screening was conducted biannually, and five rounds were planned. After clinical breast examination (CBE), two-view mammograms were obtained. True positivity, false positivity, positive predictive values (PPV) according to ACR, cancer detection rate, minimal cancer detection rate, axillary node positivity and recall rate were calculated. Breast ultrasound and biopsy were performed in suspicious cases. Results: Breast biopsy was performed in 55 patients, and 18 cancers were detected in the first round. The overall cancer detection rate was 4.8 per 1,000 women. Most of the screened women (54%) and detected cancers (56%) were in women aged 40-49. Ductal carcinoma in situ (DCIS) and stage I cancer and axillary node positivity rates were 22%, 61%, and 16.6%, respectively. The positive predictivity for biopsy was 32.7%, whereas the overall recall rate was 18.4 %. Conclusions: Preliminary results of the study suggest that population based organized screening are feasible and age of onset of mammographic screening should be 40 years in Turkey.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.8
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• pp.3229-3232
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• 2015

Background: Triple-negative breast cancer (TNBC) is known to be associated with aggressive biologic features and a poor clinical outcome. Therefore, early detection of TNBC without missed diagnosis is a requirement to improve prognosis. Preoperative ultrasound features of TNBC may potentially assist in early diagnosis as characteristics of disease. Purpose: To retrospectively evaluate the sonographic features of TNBC compared to ER (+) cancers which include HER(-) and HER2 (+), and HER2 (+) cancers which are ER (-). Materials and Methods: From June 2012 through June 2014, sonographic features of 321 surgically confirmed ER (+) cancers (n=214), HER2 (+) cancers (n=66), and TNBC (n=41) were retrospectively reviewed by two ultrasound specialists in consensus. The preoperative ultrasound and clinicopathological features were compared between the three subtypes. In addition, all cases were analyzed using morphologic criteria of the ACR BI-RADS lexicon. Results: Ultrasonographically, TNBC presented as microlobulated nodules without microcalcification (p=0.034). A lower incidence of ductal carcinoma in situ (p<0.001), invasive tumor size that is>2 cm (p=0.011) and BI-RADS category 4 (p<0.001) were significantly associated with TNBC. With regard to morphologic features of 41 TNBC cases, ultrasonographically were most likely to be masses with irregular (70.7%) microlobulated shape (48.8%), be circumscribed (17.1%) or have indistinct margins (17.1%) and parallel orientation (68.9%). Especially TNBC microlobulated mass margins were more more frequent than with ER (+) (2.0%) and HER2 (+) (4.8%) cancers. Conclusions: TNBC have specific characteristic in sonograms. Ultrasonography may be useful to avoid missed diagnosis and false-negative cases of TNBC.

• Journal of KIISE:Information Networking
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• v.41 no.4
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• pp.151-166
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• 2014

The present method of measuring QoE (Quality of Experience) was to measure the whole block from the server that provides service to the terminal that the client gets the service. In this way, it was possible to simply determine whether the quality of QoE was good or not, and when the quality of QoE became bad, there was no way of presenting solutions. Also, as QoE metrics are numerous, there has been no strict regulation on how to use. This study analyzed QoE metrics in the viewpoints of network and clients, subdivided the whole service into three phases including one from the server providing the service to the router, another from the router to the terminal getting the service, and the third from the service router to the client router, and presented QoE metric frames appropriate for each phase. Through this, in the KOREN-CERNET environment, this study designed and embodied QoE network and demonstrated stability of QoE network, reduction in client complaint settlement time, and content adjustment effect according to the network change.

• Journal of Korea Water Resources Association
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• v.31 no.2
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• pp.199-208
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• 1998

This study is on the application of TOPMDEL(Topographic based hydrologic model) Which is a distributed rainfall-runoff model to the flood runoff analysis. The test area was Wichun experimental catchment site which is mountainous mid-area (Dongok, 33.63$\textrm{km}^2$ and Goro, 109,725 $\textrm{km}^2$) and being operated by the Ministry of Construction and ransporation. A three-dimensional digital elevation model(DEM) map was constructed using a physiographic map(1/25,000) and GIS software, Arc/Info, was used to the analysis of geofraphic factors. The topographic index of Dongok and Goro subcatchment was similar. As a results of the analysis, the model was validated that the simulated peak flow of a flood runoff was fit to the observed data. For the analysis of the effects of grid size, Dongok subcatchment was divided into 100,120-,240 m grid and Goro subcatchment was divided into grid and 120,200,350 m grid. It was shown that the peak flow increased in proportion to the increases of the grid size, but peak times were constant regardless of the grid size in both of the watershed.

• Journal of Korean Society for Geospatial Information Science
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• v.19 no.3
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• pp.33-40
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• 2011

This paper presents the prediction and evaluation of landslide using LRA(logistic regression analysis) and ANN (Artificial Neural Network) methods. In order to assess the landslide, we selected Sarabong, Byeoldobong area and Mt. Song-ak in Jeju Island. Five factors which affect the landslide were selected as: slope angle, elevation, porosity, dry density, permeability. So as to predict and evaluate the landslide, firstly the weight value of each factor was analyzed by LRA(logistic regression analysis) and ANN(Artificial Neural Network) methods. Then we got two prediction maps using AcrView software through GIS(Geographic Information System) method. The comparative analysis reveals that the slope angle and porosity play important roles in landslide. Prediction map generated by LRA method is more accurate than ANN method in Jeju. From the prediction map, we found that the most dangerous area is distributed around the road and path.

• Clinical and Experimental Pediatrics
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• v.63 no.1
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• pp.20-24
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• 2020

Background: Predicting the prognosis of patients admitted to the pediatric intensive care unit (PICU) is very important in determining further management and resource allocation. The prognostication of critically ill children can be challenging; hence, accurate methods for predicting outcomes are needed. Purpose: To evaluate the role of microalbuminuria at admission as a prognostic marker in comparison to standard Pediatric Risk of Mortality (PRISM) and Pediatric Logistic Organ Dysfunction (PELOD) mortality scores in children admitted to the PICU. Methods: This cross-sectional study was conducted from January 2015 to October 2016. Eighty-four patients aged 1 month to 18 years admitted to the PICU of teaching hospitals for more than 24 hours were enrolled by convenience sampling method. Microalbuminuria was estimated by spot urinary albumin-creatinine ratio. PRISM and PELOD scores were calculated using an online calculator. Outcome measures were PICU length of stay, inotrope usage, multiorgan dysfunction, and survival. ACR was compared with mortality scores for predicting survival. Results: Microalbuminuria was present in 79.8% with a median value of 85 mg/g (interquartile range, 41.5-254 mg/g). A positive correlation was found between albumin-creatinine ratio and PICU length of stay (P=0.013, r=0.271). Albumin-creatinine ratio was significantly associated with organ dysfunction (P=0.004) and need for inotropes (P=0.006). Eight deaths were observed in the PICU. The area under the curve for mortality for albumin-creatinine ratio (0.822) was comparable to that for PRISM (0.928) and PELOD (0.877). Albumin-creatinine ratio >109 mg/g predicted mortality with a sensitivity of 87.5% and specificity of 63.2%. Conclusion: Microalbuminuria is a good predictor of PICU outcomes comparable with mortality scores.

• Journal of Korean Society of Forest Science
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• v.85 no.2
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• pp.251-259
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• 1996

This study was conducted to find out whether information based on norm activation theory affects personal norm and rule-violating behavior in a recreation setting, using the data collected from the Second Campground in Chiri-Mountain National Park in 1994. Of the total 280 questionnaires distributed, 253(90.4%) were usable for data analysis. Results showed that information did not increase awareness of consequences(AC) of their actions or ascription of responsibility(AR) for acts and consequences to themselves, and did not directly decrease the quiet time rule-violating behavior. However, it was found that respondents with high ACR(combination of AC and AR) or personal norms less violated the rule. Management implications of these findings were discussed.

• Journal of Ocean Engineering and Technology
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• v.32 no.6
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• pp.485-491
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• 2018

Because of the increase in coastal erosion problems, many studies have been conducted to prevent coastline retreat by developing low-cost, highly effective countermeasures. We developed the artificial coral reefs (ACRs) method as part of this research trend. To verify its coastal protection performance, we carried out performance tests on its wave attenuation and beach sand capture ability, which are the key barometers for this newly developed technology. In this study, three different types of methods, including natural beach, TTP, and ACRs, were used to determine the coastal protection efficiency under both ordinary and storm wave conditions. Based on the results of this study, ACRs were found to have the best wave attenuation performance and captured more than 20% of the total erosion area. This means the ACR method can be applied as a reliable countermeasure to protect a coastal zone.